Production of antisera against electrophoretically separated relaxin and immunofluorescent localization of relaxin in the porcine corpus luteum.

abstract：:Both fructose consumption and increased intracellular glucocorticoid activation have been implicated in the pathogenesis of the metabolic syndrome. Glucocorticoid activation by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) depends on hexose-6-phosphate dehydrogenase (H6PD), which physically interacts with 11β-HSD...

journal_title：Endocrinology

authors： Senesi S,Legeza B,Balázs Z,Csala M,Marcolongo P,Kereszturi E,Szelényi P,Egger C,Fulceri R,Mandl J,Giunti R,Odermatt A,Bánhegyi G,Benedetti A

更新日期：2010-10-01 00:00:00

abstract：:Analogs of the seco-steroid hormone, 1,25-dihydroxyvitamin D3 [1,25-(OH)-2D3] can preferentially stimulate genomic or nongenomic signaling pathways in osteoblasts. In this study, we used 1,25-(OH)2D3 analogs and voltage-sensitive Ca2+ channel (VSCC) ligands, including dihydropyridines (Bay K 8644 and nitrendipine), in...

journal_title：Endocrinology

authors： Khoury R,Ridall AL,Norman AW,Farach-Carson MC

更新日期：1994-12-01 00:00:00

abstract：:Stearoyl-CoA desaturase-1 (SCD-1) plays a pivotal role in an increase of triglyceride by an excess of dietary carbohydrate intake. Dietary carbohydrates increase SCD-1 gene expression in liver by sterol response element binding protein (SREBP)-1c-dependent and SREBP-1c -independent pathways. Previous report demonstrat...

journal_title：Endocrinology

authors： Hashimoto K,Ishida E,Miura A,Ozawa A,Shibusawa N,Satoh T,Okada S,Yamada M,Mori M

更新日期：2013-01-01 00:00:00

abstract：:Dehydroepiandrosterone (DHEA) fed at 0.4% in the diet is known to exert strong antihyperglycemic effects in C57BL/KsJ genetically diabetic (db/db) mice. Three of the major metabolic products of DHEA; DHEA sulfate, alpha-hydroxyetiocholanolone (alpha-ET), and beta-hydroxyetiocholanolone (beta-ET) when fed at 0.1% in th...

journal_title：Endocrinology

authors： Coleman DL,Leiter EH,Applezweig N

更新日期：1984-07-01 00:00:00

abstract：:Fibroblasts represent one of the in vivo sites of extrahepatic insulin-like growth factor I (IGF-I) production. In this study, cultured fibroblasts prepared from the skin of neonatal rats were used as a model to assess the role of serum in regulating IGF-I messenger RNA (mRNA) levels. IGF-I mRNA, as demonstrated by No...

journal_title：Endocrinology

authors： Lowe WL Jr,Kummer M,Karpen CW,Wu XD

更新日期：1990-12-01 00:00:00

abstract：:Estradiol (E) treatment of spayed macaques induces progestin receptors (PR) in pituitary gonadotropes, but not lactotropes. In contrast, levels of pituitary estrogen receptors (ER) remain constant in spayed or E-treated monkeys. In monkey pituitary cultures, the number of PR-positive cells varies depending on the dono...

journal_title：Endocrinology

authors： Sprangers SA,Fahrenbach WH,Bethea CL

更新日期：1991-04-01 00:00:00

abstract：:Differentiation of endometrial stromal cells into decidual cells is a prerequisite for successful embryo implantation. Our previous studies in the mouse have shown that bone morphogenetic protein 2 (BMP2), a morphogen belonging to the TGFβ superfamily, is essential for this differentiation process. BMP2 is markedly in...

journal_title：Endocrinology

authors： Li Q,Kannan A,Das A,Demayo FJ,Hornsby PJ,Young SL,Taylor RN,Bagchi MK,Bagchi IC

更新日期：2013-01-01 00:00:00

abstract：:Humanin (HN) is a novel 24-amino acid mitochondrial-derived peptide that has demonstrated diverse cytoprotective effects, including an emerging role in diabetes. The purpose of this study was to examine the pharmacokinetics of humanin analogues, which show great potential as therapeutic agents (HNG and the non-IGFBP-3...

journal_title：Endocrinology

authors： Chin YP,Keni J,Wan J,Mehta H,Anene F,Jia Y,Lue YH,Swerdloff R,Cobb LJ,Wang C,Cohen P

更新日期：2013-10-01 00:00:00

abstract：:Women with polycystic ovary syndrome (PCOS) and hyperandrogenism have altered hormone levels and suffer from ovarian dysfunction leading to subfertility. We have attempted to generate a model of hyperandrogenism by feeding mice chow supplemented with dehydroepiandrosterone (DHEA), an androgen precursor that is often e...

journal_title：Endocrinology

authors： Jimenez PT,Frolova AI,Chi MM,Grindler NM,Willcockson AR,Reynolds KA,Zhao Q,Moley KH

更新日期：2013-12-01 00:00:00

abstract：:FSH and IGF-I are both important determinants of testicular development and Sertoli cell function. The present studies were performed to determine the actions of FSH and IGF-I on PI3K/AKT protein kinase signaling in immature rat Sertoli cells. Primary cultures of rat Sertoli cells were prepared from 10-d-old rats. Aft...

journal_title：Endocrinology

authors： Khan SA,Ndjountche L,Pratchard L,Spicer LJ,Davis JS

更新日期：2002-06-01 00:00:00

abstract：:Insulin receptor substrate-1 (IRS-1), the primary substrate for the insulin receptor tyrosine kinase in most cells and tissues, is a key component of the insulin signaling network. Numerous studies have documented the trafficking of IRS-1 from the cell membrane to intracellular, extranuclear compartments. During the c...

journal_title：Endocrinology

authors： Boylan JM,Gruppuso PA

更新日期：2002-11-01 00:00:00

abstract：:Adult rats which have received monosodium-L-glutamate (MSG, 4 mg/g BW) on alternate days for the first 10 days of life manifest central nervous system lesions largely restricted to the retina and the arcuate nucleus of the hypothalamus in which nearly 90% of the perikarya are destroyed, leaving axons in passage intact...

journal_title：Endocrinology

authors： Greeley GH Jr,Nicholson GF,Nemeroff CB,Youngblood WW,Kizer JS

更新日期：1978-07-01 00:00:00

abstract：:In osteoblasts, retinoic acid (RA) modulates the synthesis of various proteins, including collagen. However, little is known about the effects of RA on the regulation of interstitial collagenase synthesis and collagen degradation. After treatment of primary osteoblast-enriched (Ob) cells from fetal rat calvariae with ...

journal_title：Endocrinology

authors： Varghese S,Rydziel S,Jeffrey JJ,Canalis E

更新日期：1994-06-01 00:00:00

abstract：:To study the physiological regulation of the iodothyronine 5'-deiodinases (I-5'D), we have examined the effects of some thiol blockers and of thyroid status on I-5'D activities both in vitro and in vivo. At low (less than 5 mM) concentrations of dithiothreitol, propylthiouracil (PTU) inhibited I-5'D in the brain, pitu...

journal_title：Endocrinology

authors： Goswami A,Rosenberg IN

更新日期：1990-05-01 00:00:00

abstract：:Subcellular preparations from rat liver, brain, and kidney and from human liver were tested for their ability to desulfate T3 sulfate (T3SO4). Activity was found associated with the microsomal fraction: rat liver was the most active, hydrolyzing 76 pmol/min.mg protein of T3SO4 while preparations from rat kidney and br...

journal_title：Endocrinology

authors： Kung MP,Spaulding SW,Roth JA

更新日期：1988-04-01 00:00:00

abstract：:We and others have recently shown that 1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] significantly inhibits cell proliferation and increases secretion of prostate-specific antigen (PSA) in LNCaP cells, an androgen-responsive human prostate cancer cell line. The present study was designed to investigate the possible int...

journal_title：Endocrinology

authors： Zhao XY,Ly LH,Peehl DM,Feldman D

更新日期：1997-08-01 00:00:00

abstract：:We hypothesized the existence of vagal arginine sensors in the liver which modulate arginine-induced pancreatic hormone secretion. The present study was carried out to examine the efferent pathways and receptor mechanisms from arginine sensors using selective vagotomies and autonomic drugs on the secretion of insulin ...

journal_title：Endocrinology

authors： Tanaka K,Inoue S,Nagase H,Takamura Y

更新日期：1990-10-01 00:00:00

abstract：:When colonic crypt cells isolated from intact rats are incubated with [3H]corticosterone specific nuclear binding is displaced by neither aldosterone nor the antiglucocorticoid RU38486, suggesting that [3H]corticosterone is binding to a site distinct from classical mineralocorticoid and glucocorticoid receptors. TLC r...

journal_title：Endocrinology

authors： Sheppard KE,Funder JW

更新日期：1996-08-01 00:00:00

abstract：:A substantial body of experimental evidence indicates that serotonin (5-HT) and several synthetic 5-HT receptor agonists activate the hypothalamic-pituitary-adrenal (HPA) axis. To explore the mechanism(s) by which 5-HT or 5-HT agonists enhance the activity of the HPA axis in vitro, we examined the stimulatory effects ...

journal_title：Endocrinology

authors： Calogero AE,Bagdy G,Szemeredi K,Tartaglia ME,Gold PW,Chrousos GP

更新日期：1990-04-01 00:00:00

abstract：:It has been suggested that ewes carrying the Booroola gene (Fec(B)) consistently ovulate more follicles because they recruit more primordial follicles and/or have a lower rate of atresia. If the former is correct, the pool of follicles would be depleted sooner in Fec(B) animals. We have studied follicular dynamics and...

journal_title：Endocrinology

authors： Gonzalez-Bulnes A,Souza CJ,Campbell BK,Baird DT

更新日期：2004-06-01 00:00:00

abstract：:Three distinct receptor types for natriuretic peptides (NP) have been identified in human tissue. "A" and "B" receptors initiate biological actions, whereas the "C" receptor has a clearance function. It has been proposed that the natural ligand for the B receptor is c-type natriuretic peptide (CNP), rather than atrial...

journal_title：Endocrinology

authors： Canaan-Kühl S,Jamison RL,Myers BD,Pratt RE

更新日期：1992-01-01 00:00:00

abstract：:Epididymal adipocytes were isolated from Fischer 344 rats aged 3, 6, 12, and 24 months, to study the mechanisms responsible for age-dependent diminution in cellular adrenergic responsiveness. Messenger RNA (mRNA) levels for the beta 1-, beta 2-, and beta 3-adrenergic receptors (ARs) were compared across age groups and...

journal_title：Endocrinology

authors： Gettys TW,Rohlfs EM,Prpic V,Daniel KW,Taylor IL,Collins S

更新日期：1995-05-01 00:00:00

abstract：:In muscle, physiologic hyperinsulinemia, presumably acting on endothelial cells (ECs), dilates arterioles and regulates both total blood flow and capillary recruitment, which in turn influences glucose disposal. In cultured ECs, however, supraphysiological (e.g. >or=10 nM) insulin concentrations are typically used to ...

journal_title：Endocrinology

authors： Li G,Barrett EJ,Wang H,Chai W,Liu Z

更新日期：2005-11-01 00:00:00

abstract：:Germ cells were isolated from 30-day-old rat testes and purified by a Percoll gradient. Germ cells were cultured for 24 h to eliminate contaminant cells by utilizing the property of many nongerm cells of attaching to plastic surfaces. Germ cells were subsequently seeded on top of 7-day-old Sertoli cell monolayers. RNA...

journal_title：Endocrinology

authors： Rivarola MA,Sanchez P,Saez JM

更新日期：1985-11-01 00:00:00

abstract：:Topiramate (TPM) treatment has been shown to reduce adiposity in humans and rodents. The reduction in adiposity is related to decreased food intake and increased energy expenditure. However, the molecular mechanisms through which TPM induces weight loss are contradictory and remain to be clarified. Whether TPM treatme...

journal_title：Endocrinology

authors： Caricilli AM,Penteado E,de Abreu LL,Quaresma PG,Santos AC,Guadagnini D,Razolli D,Mittestainer FC,Carvalheira JB,Velloso LA,Saad MJ,Prada PO

更新日期：2012-09-01 00:00:00

abstract：:The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into ...

journal_title：Endocrinology

authors： Clevenger CV,Sillman AL,Prystowsky MB

更新日期：1990-12-01 00:00:00

abstract：:Although Na+/H+ exchange is important to maintenance of pH and volume and thus placental-fetal homeostasis, regulation of the Na+/H+ exchange system is incompletely understood. We previously showed that Na+/H+ exchanger (NHE)1 and -3 and their regulatory factors NHERF1 and -2 were expressed in human and nonhuman prima...

journal_title：Endocrinology

authors： Pepe GJ,Burch MG,Albrecht ED

更新日期：2006-06-01 00:00:00

abstract：:High plasma levels of estradiol (E2) are associated with use of a place memory system over a response memory system. We examined whether infusing estradiol into the medial prefrontal cortex (mPFC) or anterior cingulate cortex (AC) could affect memory system bias in female rats. We also examined the ultrastructural dis...

journal_title：Endocrinology

authors： Almey A,Cannell E,Bertram K,Filardo E,Milner TA,Brake WG

更新日期：2014-11-01 00:00:00

abstract：:The disappearance of 125-I-ACTH from the circulation of 4 pregnant and fetal sheep has been followed after a single injection to the ewe or fetus. The mean metabolic clearance rate for the fetus and the ewe was 55 and 34 ml/min/kg respectively, giving a half-life in each case of about 1 min. The higher fetal than mate...

journal_title：Endocrinology

authors： Jones CT,Luther E,Ritchie JW,Worthington D

更新日期：1975-01-01 00:00:00

abstract：:Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize ...

journal_title：Endocrinology

authors： Furlow JD,Ahrens H,Mueller GC,Gorski J

更新日期：1990-09-01 00:00:00