Antisera to a synthetic peptide recognize native and denatured rat estrogen receptors.

abstract：:Hydrogen peroxide acts as electron acceptor in the oxidative reactions (iodination and coupling) by which the thyroid hormones are formed. Regulation of the generation of H2O2 was studied in monolayer cultures of the FRTL-5 rat thyroid cell line and in primary monolayer cultures of porcine thyroid cells. Both cell typ...

journal_title：Endocrinology

authors： Björkman U,Ekholm R

更新日期：1992-01-01 00:00:00

abstract：:Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of the u...

journal_title：Endocrinology

authors： Fok KL,Bose R,Sheng K,Chang CW,Katz-Egorov M,Culty M,Su S,Yang M,Ruan YC,Chan HC,Iavarone A,Lasorella A,Cencic R,Pelletier J,Nagano M,Xu W,Wing SS

更新日期：2017-11-01 00:00:00

abstract：:We have recently identified gonadotropes as target cells for ATP action via ATP receptors of the P2U subtype. The present studies have used gonadotrope-derived alpha T3-1 cells to examine the possible signaling mechanisms subserving ATP action in gonadotropes. Addition of ATP produced a biphasic intracellular Ca2+ (Ca...

journal_title：Endocrinology

authors： Chen ZP,Kratzmeier M,Poch A,Xu S,McArdle CA,Levy A,Mukhopadhyay AK,Lightman SL

更新日期：1996-01-01 00:00:00

abstract：:Steroidogenic acute regulatory protein (StAR) synthesis and steroidogenesis are stimulated by activation of divergent signaling pathways in the adrenal cortex. The two major physiological regulators of aldosterone synthesis in the adrenal zona glomerulosa are angiotensin II (AII) and extracellular K+, which both media...

journal_title：Endocrinology

authors： Clark BJ,Combs R

更新日期：1999-10-01 00:00:00

abstract：:The induction of hepatic drug metabolizing enzymes alters not only the metabolism of the xenobiotic substances that induce them but also the metabolism of various endogenous hormones. The xenobiotic receptor constitutive androstane receptor (CAR) (NR1I3) mediates the well-studied induction of CYP2B genes and other dru...

journal_title：Endocrinology

authors： Qatanani M,Zhang J,Moore DD

更新日期：2005-03-01 00:00:00

abstract：:Mature male rat liver cytosol contains a moderate affinity and capacity estrogen-binding protein in at least a 200-fold higher level than mature female or immature male rat liver cytosol. Binding of estradiol to this protein is very rapid, is stabilized by EDTA, and is inhibited by divalent cations. This is the major ...

journal_title：Endocrinology

authors： Dickson RB,Aten RF,Eisenfeld AJ

更新日期：1978-11-01 00:00:00

abstract：:Pituitary glands from ovariectomized F-344 rats bearing diethylstilbestrol-containing capsules for 70-100 days were dissociated and separated into three size classes by unit gravity sedimentation. The average percentage of large mammotrophs in fraction I was 86 +/- (SE) 2%, the percentage of intermediate-sized mammotr...

journal_title：Endocrinology

authors： Hymer WC,Motter KA

更新日期：1988-05-01 00:00:00

abstract：:Oxytocin plays a pivotal role in rat parturition, acting within the brain to facilitate its own release in the supraoptic nucleus (SON) and paraventricular nucleus, and to stimulate maternal behavior. We investigated oxytocin receptor (OTR) expression and activation perinatally. Using a (35)S-labeled riboprobe complem...

journal_title：Endocrinology

authors： Meddle SL,Bishop VR,Gkoumassi E,van Leeuwen FW,Douglas AJ

更新日期：2007-10-01 00:00:00

abstract：:We and others have recently shown that 1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] significantly inhibits cell proliferation and increases secretion of prostate-specific antigen (PSA) in LNCaP cells, an androgen-responsive human prostate cancer cell line. The present study was designed to investigate the possible int...

journal_title：Endocrinology

authors： Zhao XY,Ly LH,Peehl DM,Feldman D

更新日期：1997-08-01 00:00:00

abstract：:Leptin has been shown to activate multiple signaling molecules in cultured cells, including Janus kinase-2, STAT (signal transducer and activator of transcription) proteins, and mitogen-activated protein kinase, and to stimulate the DNA-binding activity of STAT3 in mouse hypothalamus. In this study, the activation of ...

journal_title：Endocrinology

authors： McCowen KC,Chow JC,Smith RJ

更新日期：1998-11-01 00:00:00

abstract：:The subunit structure of mammalian cytoplasmic progesterone receptors (PR) has been difficult to study because these proteins are subject to in vitro proteolysis; the structure of nuclear PR is unknown. We have now developed an in situ photoaffinity labeling method for PR that permits study of their subunits with mini...

journal_title：Endocrinology

authors： Horwitz KB,Alexander PS

更新日期：1983-12-01 00:00:00

abstract：:Apoptosis is the process of cellular self-destruction, and genes such as bcl-2 and bax are known to inhibit and promote apoptosis, respectively. In this study, we show that apoptosis can be induced in pancreatic beta-cell lines, and we investigate the apoptotic pathways through the bcl-2 and bax genes and intracellula...

journal_title：Endocrinology

authors： Mizuno N,Yoshitomi H,Ishida H,Kuromi H,Kawaki J,Seino Y,Seino S

更新日期：1998-03-01 00:00:00

abstract：:Since specific binding to receptors and biological effects of insulin and insulin-like growth factors (IGFs) are demonstrable soon after the neural tube closes and organogenesis begins in the chick embryo, in the present study we have analyzed the structural characteristics and specificity of the receptors for insulin...

journal_title：Endocrinology

authors： Bassas L,de Pablo F,Lesniak MA,Roth J

更新日期：1987-10-01 00:00:00

abstract：:We previously reported that cAMP inhibits autocrine IGF-I gene expression in rat C6 glioma cells. In this study we examined the influence of cAMP on IGF-binding protein gene expression in C6 cells. cAMP potently inhibited IGF-binding protein-3 mRNA and, to a lesser extent, IGF-binding protein-4 mRNA and transiently st...

journal_title：Endocrinology

authors： Wang L,Ma X,Yeh LC,Adamo ML

更新日期：2001-09-01 00:00:00

abstract：:Thyroid papillary carcinoma is the most common type of endocrine cancer. It is frequently associated with genetic alterations leading to activation of the MAPK signaling pathway. The two most frequently affected genes, BRAF and RET, are activated by either point mutation or as a result of chromosomal rearrangement. Th...

journal_title：Endocrinology

authors： Ciampi R,Nikiforov YE

更新日期：2007-03-01 00:00:00

abstract：:Although prolactinomas can be effectively treated with dopamine agonists, about 20% of patients develop dopamine resistance or tumor recurrence after surgery, indicating a need for better understanding of underlying disease mechanisms. Although estrogen-induced rat prolactinomas have been widely used to investigate th...

journal_title：Endocrinology

authors： Tong Y,Zheng Y,Zhou J,Oyesiku NM,Koeffler HP,Melmed S

更新日期：2012-08-01 00:00:00

abstract：:The uptake of [125I]triiodothyroacetic acid ([125I]Triac) in anterior pituitary cells was investigated and compared with that of [125I]T3. Furthermore, the effects of Triac, T3, and T4 on TSH release were compared. Cells isolated from adult male Wistar rats were cultured for 3 days in medium with 10% fetal calf serum....

journal_title：Endocrinology

authors： Everts ME,Visser TJ,Moerings EP,Docter R,van Toor H,Tempelaars AM,de Jong M,Krenning EP,Hennemann G

更新日期：1994-12-01 00:00:00

abstract：:Gene expression of fibroblast growth factor-9 (FGF9) is decreased in granulosa cells (GC) of cystic follicles compared with normal dominant follicles in cattle. The objectives of this study were to investigate the effects of FGF9 on GC steroidogenesis, gene expression, and cell proliferation and to determine the hormo...

journal_title：Endocrinology

authors： Schreiber NB,Spicer LJ

更新日期：2012-09-01 00:00:00

abstract：:A 28,000 mol wt vitamin D-dependent calcium-binding protein (CaBP), first isolated from avian intestine, has recently been shown to exist in kidney and other tissues. To study the mechanism regulating the production of renal CaBP, we used primary cultures of chick kidney cells as an in vitro model. Renal cortical tubu...

journal_title：Endocrinology

authors： Craviso GL,Garrett KP,Clemens TL

更新日期：1987-03-01 00:00:00

abstract：:We recently reported that bone morphogenetic proteins (BMPs) 2 and 4 can stimulate FSHbeta-subunit (Fshb) transcription alone and in synergy with activins. We further showed that BMP2 signals via the BMP type IA receptor (or activin receptor-like kinase 3) to mediate its effects. However, the intracellular mechanisms ...

journal_title：Endocrinology

authors： Ho CC,Bernard DJ

更新日期：2010-07-01 00:00:00

abstract：:The procession of round spermatids through stages VII and VIII of the rat spermatogenic cycle is critically dependent on testosterone (T). When intratesticular T levels are reduced, round spermatids appear to slough from the seminiferous epithelium, resulting in the disappearance of elongated spermatids. We hypothesiz...

journal_title：Endocrinology

authors： Perryman KJ,Stanton PG,Loveland KL,McLachlan RI,Robertson DM

更新日期：1996-09-01 00:00:00

abstract：:Circulating levels of insulin-like growth factor-I (IGF-I) increase during puberty, concurrent with an increase in the levels of GH and the gonadal steroids. The relationship between the changes observed in IGF-I and testosterone (T) levels are not understood. This study was designed to determine whether T has a direc...

journal_title：Endocrinology

authors： Phillip M,Palese T,Hernandez ER,Roberts CT Jr,LeRoith D,Kowarski AA

更新日期：1992-05-01 00:00:00

abstract：:New insulin-secreting cell lines (INS-1 and INS-2) were established from cells isolated from an x-ray-induced rat transplantable insulinoma. The continuous growth of these cells was found to be dependent on the reducing agent 2-mercaptoethanol. Removal of this thiol compound caused a 15-fold drop in total cellular glu...

journal_title：Endocrinology

authors： Asfari M,Janjic D,Meda P,Li G,Halban PA,Wollheim CB

更新日期：1992-01-01 00:00:00

abstract：:Hormonal induction of steroidogenesis in the adrenal and gonads is dependent on the synthesis and function of the steroidogenic acute regulatory protein (StAR). As a first approach to investigate the role of translation in the control of StAR expression, we examined StAR protein synthesis and steroid production in MA-...

journal_title：Endocrinology

authors： Clark BJ,Combs R,Hales KH,Hales DB,Stocco DM

更新日期：1997-11-01 00:00:00

abstract：:The dynamics of dopamine (DA) action on PRL release was studied in superfused rat anterior pituitary cell aggregates, cultured for for 5 days either in conventional or in serum-free defined medium. In aggregates cultured in conventional medium 0.1-1 nM DA applied for 20 min provoked a rapid and concentration-dependent...

journal_title：Endocrinology

authors： Denef C,Baes M,Schramme C

更新日期：1984-04-01 00:00:00

abstract：:It is unclear whether the maturation of corticotrophs from the fetal to the adult type in the fetal sheep pituitary in late gestation is associated with changes in the sensitivity of the fetal pituitary to corticotrophic secretagogues and in the form of ACTH-containing peptides (IR-ACTH) secreted into the circulation....

journal_title：Endocrinology

authors： Ozolins IZ,Antolovich GC,Browne CA,Perry RA,Robinson PM,Silver M,McMillen IC

更新日期：1991-10-01 00:00:00

abstract：:Various neuropeptides have recently been isolated from the thymus, suggesting an interaction between the neuroendocrine and immune systems. We report here that the human thymus synthesizes a further peptide hormone, namely atrial natriuretic peptide (ANP). This is documented by the following findings. ANP and its proh...

journal_title：Endocrinology

authors： Vollmar AM,Schulz R

更新日期：1990-05-01 00:00:00

abstract：:Prenatal stress is associated with altered behavior and hypothalamic-pituitary-adrenal (HPA) axis function postnatally. Recent studies suggest that these outcomes are dependent on the timing of the prenatal stress. The majority of these studies have been carried out in male offspring. We hypothesized that a short peri...

journal_title：Endocrinology

authors： Kapoor A,Matthews SG

更新日期：2008-12-01 00:00:00

abstract：:The present study was designed to characterize the interaction of calcium and PTH in the control of renin release in isolated rat kidneys perfused in a closed circuit at constant flow. Kidneys were rendered nonfiltering using low perfusion pressures (70 mm Hg) and a hyperoncotic perfusate (100 g/liter BSA). Under thes...

journal_title：Endocrinology

authors： Helwig JJ,Musso MJ,Judes C,Nickols GA

更新日期：1991-09-01 00:00:00

abstract：:We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as asses...

journal_title：Endocrinology

authors： Ueta Y,Levy A,Chowdrey HS,Lightman SL

更新日期：1995-10-01 00:00:00