Abstract:
:Apoptosis is the process of cellular self-destruction, and genes such as bcl-2 and bax are known to inhibit and promote apoptosis, respectively. In this study, we show that apoptosis can be induced in pancreatic beta-cell lines, and we investigate the apoptotic pathways through the bcl-2 and bax genes and intracellular Ca2+. Serum deprivation induces apoptosis in the MIN6 and RINm5F pancreatic beta-cell lines, and alters the bcl-2 messenger RNA (mRNA) and protein. KCl, BayK, A23187, and ionomycin elicit an elevation of cytosolic/nuclear Ca2+, which, however, is insufficient to evoke apoptosis or to alter bcl-2 or bax mRNA expression in MIN6 cells. The extracellular Ca2+ chelators, EGTA and 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetrapotassium salt, hydrate, evoke apoptosis and also alter the ratio of bcl-2 to bax mRNA and protein concomitantly with the depletion of cytosolic/nuclear Ca2+. This indicates that there are at least two apoptotic pathways in pancreatic beta-cells: through serum deprivation and through a decrease in cytosolic/nuclear Ca2+. MIN6 cells exhibit reduced insulin secretion induced by glucose regardless of the molecular pathway of apoptosis. Apoptosis in pancreatic beta-cells, therefore, may be closely related to the impairment of insulin secretion in certain pathological conditions such as diabetes mellitus.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Mizuno N,Yoshitomi H,Ishida H,Kuromi H,Kawaki J,Seino Y,Seino Sdoi
10.1210/endo.139.3.5798subject
Has Abstractpub_date
1998-03-01 00:00:00pages
1429-39issue
3eissn
0013-7227issn
1945-7170journal_volume
139pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Previous studies have provided evidence that tumors associated with humoral hypercalcemia of malignancy produce a factor that shares certain biological properties with, but is not, native PTH. In the present study, media from Xenopus oocytes microinjected with polyadenylated RNA prepared from three human or animal tum...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1661
更新日期:1985-10-01 00:00:00
abstract::Decidual/trophoblast PRL-related protein (d/tPRP) is a member of the PRL gene family and is dually expressed in uterine and placental tissues in a highly coordinated pattern during pregnancy. In the present study, we describe the isolation and characterization of the d/tPRP gene. A lambda DASH II Wistar-Kyoto rat geno...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.6.5155
更新日期:1997-06-01 00:00:00
abstract::Animals shipped from commercial suppliers to laboratories are exposed to a wide variety of stressors. Female C57Bl6/J mice shipped during the peripubertal/adolescent period (6 wk old) display lower levels of female sexual behavior in response to estradiol and progesterone injections after ovariectomy when tested in ad...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1595
更新日期:2009-05-01 00:00:00
abstract::Nutritional programming, taking place in utero or early after birth, is closely linked with metabolic and appetite disorders in adulthood. Following the hypothesis that nutritional programming impacts hypothalamic neuronal organization, we report on discrepancies of multiple molecular and cellular early events that ta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0893
更新日期:2010-02-01 00:00:00
abstract::Clinically, approximately 30% of women who receive synthetic glucocorticoids (sGC) for risk of preterm labor carry to term. In vitro studies have shown that sGC acutely regulate the placental system A amino acid transporter, but there are no comparable data in vivo. Hence, the objective of our study was to examine the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0104
更新日期:2011-09-01 00:00:00
abstract::Impairments in the estradiol (E2)-induced surge of LH occur as aging female rodents lose reproductive cycles. These and other impairments can be substantially attenuated by ovariectomy of the young adult. In female rats, plasma PRL tends to increase with age, whereas in male rats, GH tends to decrease; PRL is regulate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-813
更新日期:1985-02-01 00:00:00
abstract::Thyroid hormone (TH) is required for frog metamorphosis, and corticosterone (CORT) increases TH signaling to accelerate metamorphic progression. However, a requirement for CORT in metamorphosis has been difficult to assess prior to the recent development of gene-editing technologies. We addressed this long-standing qu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa193
更新日期:2020-12-01 00:00:00
abstract::Although the pituitary-grafted rat is a classic model of chronic PRL excess, the presence of somatotropes in grafted pituitary tissue indicates a potential for GH secretion. The current study was designed to investigate GH-releasing hormone (GRH)-induced GH secretion and beta-adrenergic inhibition of GH release in ani...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2273
更新日期:1989-11-01 00:00:00
abstract::To study possible mechanisms controlling diurnal changes in corticosterone (CORT) levels, we tested the CORT responses to ACTH in the morning (AM) and evening (PM) in male Wistar and Sprague-Dawley rats. Rat ACTH-(1-39) or human ACTH-(1-24) (3.75-15 ng/rat) was given as an iv bolus or an intraarterial infusion to (un)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.2.8593800
更新日期:1996-02-01 00:00:00
abstract::Hyperandrogenism is the central feature of polycystic ovary syndrome (PCOS). Due to the intricate relationship between hyperandrogenism and insulin resistance in PCOS, 50%-70% of these patients also present with hyperinsulinemia. Metformin, an insulin sensitizer, has been used to reduce insulin resistance and improve ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1733
更新日期:2016-01-01 00:00:00
abstract::cAMP is involved in the regulation of secretory activity in lactotrope, thyrotrope, and gonadotrope cells. The present study examined whether pulsatile or intermittent changes in cAMP are more effective than a continuous stimulation in increasing pituitary hormone gene expression. Pituitaries from adult female rats we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1280210
更新日期:1992-12-01 00:00:00
abstract::Mammary glands from second generation vitamin D-deficient mice and rats were examined for their ability to make the major milk proteins, casein and alpha-lactalbumin, both in vivo and in vitro. The glands from the rachitic animals were morphologically indistinguishable from those of age-matched controls. When placed i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-3-865
更新日期:1987-09-01 00:00:00
abstract::Human IGF binding protein-3 (hIGFBP-3) overexpression in mice causes hyperglycemia, but its effect on β-cell function is unknown. We compared wild-type mice with mice overexpressing hIGFBP-3 [phoshoglycerate kinase (PGK)BP3] and mutant (Gly⁵⁶/Gly⁸⁰/Gly⁸¹)hIGFBP-3 devoid of IGF binding affinity (PGKmBP3). Intraperitone...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1324
更新日期:2011-06-01 00:00:00
abstract::The purpose of this study was to examine how the physical characteristics of light influence the short photoperiod-induced atrophy of the reproductive system. In the first experiment, groups of Syrian hamsters were exposed to a 14-h light, 10-h dark (14:10) photoperiod, a 11:13 photoperiod or a 11:13 photoperiod exten...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-648
更新日期:1986-08-01 00:00:00
abstract::Studies in rodents demonstrate that the mitogen, IGF-I, stimulates intestinal peptide YY (PYY) expression. To investigate whether the stimulatory influence of IGF-I is exerted at the level of gene transcription, rat PYY 5'-upstream sequences (-2800/+37 bp, -770/+37 bp, -127/+37 bp) fused to the firefly luciferase (luc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0770
更新日期:2004-02-01 00:00:00
abstract::We have recently demonstrated that GnRH-stimulated gonadotropin secretion is augmented by coadministration with neuropeptide-Y (NPY) in anterior pituitaries removed in the afternoon from proestrous, but not metestrous, rats. To test the hypothesis that these effects of NPY are due to an interaction with progesterone (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828530
更新日期:1995-01-01 00:00:00
abstract::Exposure of the ileum to nutrients markedly inhibits several upper gastrointestinal functions. Hormonal peptides of the ileal wall, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT), are thought to play a role in this negative feedback mechanism. The present study was conducted to comparativ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.9.6202
更新日期:1998-09-01 00:00:00
abstract::Bone remodeling is a complex process of coordinated resorption and formation of bone, which is regulated by systemic hormones and by local factors. We have previously shown that the peptide hormone adrenomedullin is mitogenic to osteoblastic cells in vitro and that it promotes bone growth in vivo. The aim of the prese...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8152
更新日期:2001-05-01 00:00:00
abstract::Rat epididymal fat cells were isolated and incubated with 25 microunits insulin/ml for 15 minutes. Insulin induced a significant shift in the saturated/unsaturated fatty acid ratio of the cerebroside fraction isolated from fat cell ghosts. This change was due to a decrease of saturated fatty acids and an increase of u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-2014
更新日期:1981-05-01 00:00:00
abstract::Concern that some chemicals in our environment may affect human health by disrupting normal endocrine function has prompted research on interactions of environmental contaminants with steroid hormone receptors. We compared the activity of 2,2-bis-(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), an estrogenic metabolite...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7191
更新日期:1999-12-01 00:00:00
abstract::Steroidogenic acute regulatory protein (StAR) synthesis and steroidogenesis are stimulated by activation of divergent signaling pathways in the adrenal cortex. The two major physiological regulators of aldosterone synthesis in the adrenal zona glomerulosa are angiotensin II (AII) and extracellular K+, which both media...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7085
更新日期:1999-10-01 00:00:00
abstract::Using a Leydig tumor cell line (MA-10) transiently transfected with the human lutropin receptor (hLHR) and mutants thereof, we examined the identity of the G proteins activated by the agonist-engaged hLHR-wild type (wt) and by three of its naturally occurring constitutively active mutants. Two of the mutants examined,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0365
更新日期:2003-09-01 00:00:00
abstract::The DHCR24 gene encodes an enzyme catalyzing the last step of cholesterol biosynthesis, the conversion of desmosterol to cholesterol. To elucidate the physiological significance of cholesterol biosynthesis in mammalian cells, we investigated proliferation of mouse embryonic fibroblasts (MEFs) prepared from DHCR24(-/-)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1426
更新日期:2006-06-01 00:00:00
abstract::The GT1 GnRH cell lines express all three subunits of the cyclic nucleotide-gated (CNG) channels (CNG2, 4.3 and 5) expressed in olfactory neurons. We investigated using in situ hybridization and double immunofluorescence whether endogenous GnRH neurons in therat also express CNG channel subunits. Sections from male an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8951
更新日期:2002-06-01 00:00:00
abstract::The influence of the catecholamine hormones and some of their metabolites on the enzymatic degradation of met-enkephalin by rat brain homogenates has been evaluated. The enzyme activity was determined by the release of [125I]-tyrosine from radioiodinated met-enkephalin following Porapak Q column chromatography. Each o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-1-291
更新日期:1982-01-01 00:00:00
abstract::We have recently reported that pretreatment with electroacupuncture (EA) at stomach meridian point 36 (St36) prevents the chronic cold-stress increase in the hypothalamus-pituitary-adrenal axis (HPA), an action that may be under central control. Given that treatment for stress-related symptoms usually begins after ons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/EN.2015-1018
更新日期:2015-10-01 00:00:00
abstract::Characterization of glycoconjugates synthesized by polarized immature rabbit uterine epithelial (UE) cells in vitro, their vectorial patterns of secretion, and regulation by ovarian steroid hormones are reported. Large (mol wt, greater than 230 kDa) sialomucoglycoproteins and hyaluronate were primarily (86-96%) secret...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1727700
更新日期:1992-01-01 00:00:00
abstract::Orally delivered salt stimulates renal salt excretion more effectively than does iv delivered salt. Although the mechanisms that underlie this "postprandial natriuresis" are poorly understood, the peptide uroguanylin (UGn) is thought to be a key mediator. However, the lack of selective assays for UGn gene products has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1725
更新日期:2008-09-01 00:00:00
abstract::Equine CG (eCG) both radioiodinated and unlabeled, was injected iv into male rats, and the disappearance of the hormone from the circulation was studied. The uptake and inactivation of the hormone by various tissues was also examined. The disappearance curve of eCG consisted of two exponential components with apparent...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-4-1242
更新日期:1981-10-01 00:00:00
abstract::The present studies were conducted to further evaluate inositol phosphate formation and metabolism in prostaglandin F2 alpha (PGF2 alpha)-stimulated bovine luteal cells. Corpora lutea were dispersed with collagenase, and luteal cells were prelabeled for 3 h with [3H]inositol. Inositol phosphates produced in response t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1519
更新日期:1991-03-01 00:00:00