Milk protein synthesis by mammary glands of vitamin D-deficient mice.

Abstract:

:Mammary glands from second generation vitamin D-deficient mice and rats were examined for their ability to make the major milk proteins, casein and alpha-lactalbumin, both in vivo and in vitro. The glands from the rachitic animals were morphologically indistinguishable from those of age-matched controls. When placed in explant culture, glands from vitamin D-deficient mice and rats underwent DNA synthesis at a rate comparable to that of glands from the vitamin D-replete controls. However, the hormonally induced synthesis of casein and alpha-lactalbumin was significantly reduced in explants of glands from rachitic vs. control animals. The reduction in casein-synthesizing ability by mouse mammary gland explants was not reversed by the addition of 10(-8) or 10(-6) M 1,25-dihydroxycholecalciferol to the culture medium, but could be reversed by pretreating the vitamin D-deficient mice with the D metabolite in vivo for 10 days before the onset of culture. The decrease in milk protein synthesis in culture is paralleled in vivo by a decrease in milk protein content in the milk and lactating mammary glands of vitamin D-deficient mice and rats. Both in vivo and in vitro, it is the two highest mol wt caseins that are most affected by the lack of vitamin D in the diet. These data suggest that vitamin D does not play a fundamental role in growth and morphological development of the normal mammary gland, but, rather, it is important in maintenance of full hormonally induced functional differentiation of the mature gland.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Bhattacharjee M,Wientroub S,Vonderhaar BK

doi

10.1210/endo-121-3-865

subject

Has Abstract

pub_date

1987-09-01 00:00:00

pages

865-74

issue

3

eissn

0013-7227

issn

1945-7170

journal_volume

121

pub_type

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