Abstract:
:T(4) activation into T(3) is catalyzed by type 2 deiodinase (D2) in the brain. The rapid induction of D2 in astrocytes by transient brain ischemia has prompted us to explore the effects of hypoxia on D2 in cultures of astrocytes. Hypoxia (2.5% O(2)) of cultured astrocytes increased D2 activity, alone or in association with agents stimulating the cAMP pathway. Hypoxia had no effect on D2 mRNA accumulation. Cycloheximide did not block the effect of hypoxia on D2 activity and D2 half-life was enhanced under hypoxia demonstrating a posttranslational action of hypoxia. Furthermore, the D2 activity increase by hypoxia was not additive with the increase promoted by the proteasome inhibitor carbobenzoxy-L-leucyl-L-leucyl-L-leucinal (MG132). This strongly suggests that hypoxia leads to stabilization of D2 by slowing its degradation by the proteasome pathway. Hypoxia, in contrast to MG132, did not block the T(4)-induced D2 inactivation. A contribution of prolyl hydroxylase to the hypoxia effects on D2 was also suggested on the basis of increased D2 activity after addition of different prolyl hydroxylase inhibitors (cobalt chloride, desferrioxamine, dimethyloxalylglycine, dimethylsuccinate). Specific inhibitors of ERK, p38 MAPK, or phosphatidylinositol 3-kinase pathways were without any effect on hypoxia-increased D2 activity, eliminating their role in the effects of hypoxia. Interestingly, diphenyleneiodonium, an inhibitor of nicotinamide adenine dinucleotide phosphate oxidase inhibited the hypoxia-increased D2 indicating a role for some reactive oxygen species in the mechanism of D2 increase. Further studies are required to clarify the precise molecular mechanisms involved in the D2 stabilization by hypoxia.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lamirand A,Mercier G,Ramaugé M,Pierre M,Courtin Fdoi
10.1210/en.2007-0625subject
Has Abstractpub_date
2007-10-01 00:00:00pages
4745-53issue
10eissn
0013-7227issn
1945-7170pii
en.2007-0625journal_volume
148pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::A surge of GnRH release signals the LH surge that triggers ovulation. The GnRH surge is dependent on a switch in estradiol feedback from negative to positive and, in rodents, a daily neural signal, likely from the suprachiasmatic nuclei. Vasoactive intestinal polypeptide (VIP) may be involved in suprachiasmatic nuclei...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1098
更新日期:2008-06-01 00:00:00
abstract::Insulin and epidermal growth factor (EGF) share a number of metabolic actions, including stimulation of protein synthesis and growth in certain tissues, and activation of apparent receptor tyrosine kinase activities. We have shown that insulin and EGF promote the phosphorylation of a number of intracellular proteins i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-141
更新日期:1984-01-01 00:00:00
abstract::Gene expression of fibroblast growth factor-9 (FGF9) is decreased in granulosa cells (GC) of cystic follicles compared with normal dominant follicles in cattle. The objectives of this study were to investigate the effects of FGF9 on GC steroidogenesis, gene expression, and cell proliferation and to determine the hormo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1003
更新日期:2012-09-01 00:00:00
abstract::To evaluate the relative contribution of the GH/IGF axis to the development of peak bone mineral density (BMD), we measured skeletal changes in IGF-I knockout (KO), IGF-II KO, and GH-deficient lit/lit mice and their corresponding control mice at d 23 (prepubertal), 31 (pubertal), and 56 (postpubertal) in the entire fe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220948
更新日期:2003-03-01 00:00:00
abstract::Obesity and its associated comorbidities are of major worldwide concern. It is now recognized that there are a number of metabolically distinct pathways of obesity development. The present paper investigates the effect of moderate daily exercise on the underlying mechanisms of one such pathway to obesity, through inte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1035
更新日期:2009-01-01 00:00:00
abstract::IGF-I and IGF-II play important roles in growth and development via interactions with cell-surface receptors; however, in nature, IGFs are sequestered by at least six soluble, high-affinity IGF-binding proteins (IGFBPs), namely IGFBPs 1-6. Herein, we demonstrate that the stromal cell-derived extracellular matrix-degra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0636
更新日期:2004-02-01 00:00:00
abstract::Recent studies from our laboratory (Endocrinology 136:4762-4768, 1995) demonstrating that the expression of cAMP-dependent nuclear transcription factor CREB (cAMP response element binding protein) is lost following ovulation in macaques has revealed a novel mechanism by which the cytoplasmic and nuclear actions the cA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5346
更新日期:1997-08-01 00:00:00
abstract::There are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium L-glutamate (MSG). In our study, newborn male pups were ip injected with MSG (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0640
更新日期:2006-03-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is expressed in many neuronal and non-neuronal tissues during development as well as in adult animals. GDNF signaling is mediated through a two-component system consisting of the so called GDNF receptor-alfa (GFRalpha1) which binds to GDNF. Thereafter this complex bin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7548
更新日期:2000-05-01 00:00:00
abstract::It is still controversial whether the bone-derived hormone fibroblast growth factor-23 (FGF23) has additional physiological functions apart from its well-known suppressive actions on renal phosphate reabsorption and vitamin D hormone synthesis. Here we analyzed premature aging, mineral homeostasis, carbohydrate metabo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1878
更新日期:2012-04-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) is a newly discovered neuropeptide that is present in high amounts in hypothalamic neuroendocrine neurons and potently stimulates the accumulation of cAMP within cells of the anterior pituitary. We have employed several specific antisera recognizing different ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.9.7649120
更新日期:1995-09-01 00:00:00
abstract::The PRL-stimulating effect of metoclopramide (2-methoxy-5-chloroprocainamide) was examined in normal and adrenalectomized male rats pretreated with DL-p-chlorophenylalanine methyl ester (PCPA), an inhibitor of serotonin biosynthesis. PCPA significantly reduced the hypothalamic content of serotonin, increased basal PRL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1622
更新日期:1981-05-01 00:00:00
abstract::VEGF is produced by osteoblasts and has been postulated to function as an angiogenic stimulus during normal skeletal development and in fracture repair. In this study, we characterized the molecular mechanisms by which experimental hypoxia increases VEGF mRNA in human MG63 osteoblast-like cells. Exposure of MG63 cells...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.8112
更新日期:2001-02-01 00:00:00
abstract::Female Wistar-Furth rats were implanted sc with GH3 rat pituitary tumor cells. Tumors were palpable by 4 weeks, and animals were killed periodically from 5-9 weeks. Tumor-bearing rats (n = 10) were heavier than their respective controls, reaching a weight of 372 +/- 3 by 9 weeks vs. 195 +/- 5 g in controls (mean +/- S...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-915
更新日期:1986-03-01 00:00:00
abstract::The TSH receptor (TSHR) hinge region, the least well understood component, bridges the leucine-rich repeat and transmembrane domains. We report data on clusters of hinge charged residues the mutation of which to Ala is compatible with cell surface expression and normal, or near normal, TSH binding affinity yet with a ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1376
更新日期:2012-10-01 00:00:00
abstract::MCRs of testosterone (T) and 5 alpha-dihydrotestosterone (DHT) were determined in the morning and evening in four chronically chaired adult rhesus monkeys (Macaca mulatta) by means of steady state infusion of [4-14C]testosterone and [1,2-3H]dihydrotestosterone. Morning and evening MCRs were comparable for both T and D...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-2-402
更新日期:1979-08-01 00:00:00
abstract::The nuclear receptor peroxisome proliferator-activated receptor (PPAR)gamma modulates the expression of numerous genes involved in glucose and lipid homeostasis and plays a critical role in adipocyte differentiation. Expression of uncoupling protein (UCP)1, which is necessary for thermogenesis, is strongly stimulated ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0321
更新日期:2004-08-01 00:00:00
abstract::Previous studies showed that epidermal growth factor (EGF) and TGFalpha mimic the action of LH on the resumption of oocyte maturation. We tested whether EGF-like agents, such as amphiregulin (AR), epiregulin (ER), and betacellulin (BTC), also mediate the LH stimulation of the ovulatory response in the rat. LH induced ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0588
更新日期:2005-01-01 00:00:00
abstract::Reduced cellular immune responses and altered cytokine production by cells from mice exposed to thermal injury are minimized if dehydroepiandrosterone (DHEA) is administered after experimental burn injury in mice. An analysis of similar tests of immune function developed by mice given the antiglucocorticoid, 17 beta-h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.2.7835270
更新日期:1995-02-01 00:00:00
abstract::Humans are routinely exposed to bisphenol A (BPA), an estrogenic chemical present in food and beverage containers, dental composites, and many products in the home and workplace. BPA binds both classical nuclear estrogen receptors and facilitates membrane-initiated estrogenic effects. Here we explore the ability of en...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0189
更新日期:2006-08-01 00:00:00
abstract::PRL has been reported to exist as a number of mol wt and charge variants. We have attempted to determine the relationships among the charge and lower mol wt variants by two-dimensional gel analysis of pituitary and pituitary cell extracts. Silver-stained gels of the extracts showed three major charge isoforms of 24,00...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1565
更新日期:1985-10-01 00:00:00
abstract::We reported previously that chromogranin A (Chga) knockout (KO) mice are hypertensive and hyperadrenergic. Here we sought to determine the basis of such alterations by probing physiological, biochemical, and pharmacological responses to perturbations of the autonomic nervous system. In the conscious state, KO mice had...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0429
更新日期:2009-11-01 00:00:00
abstract::A novel transcript of the GH gene has been identified in ocular tissues of chick embryos. It is, however, unknown whether this transcript (small chicken GH, scGH) is translated. This possibility was therefore assessed. The expression of scGH mRNA was confirmed by RT-PCR, using primers that amplified a 426-bp cDNA of i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1070
更新日期:2007-01-01 00:00:00
abstract::One day of fasting suppresses pulsatile LH secretion in adult male rhesus monkeys. In the present study, we determined whether the hypothalamic-pituitary-adrenal (HPA) axis is activated by 1 day of fasting, and if there is any evidence that an increase in the activity of the HPA axis causes the fasting-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8389280
更新日期:1993-06-01 00:00:00
abstract::The influence of the catecholamine hormones and some of their metabolites on the enzymatic degradation of met-enkephalin by rat brain homogenates has been evaluated. The enzyme activity was determined by the release of [125I]-tyrosine from radioiodinated met-enkephalin following Porapak Q column chromatography. Each o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-1-291
更新日期:1982-01-01 00:00:00
abstract::The aim of this study was to investigate the direct effect of leptin on GH gene expression and secretion and the role of nitric oxide as a possible mediator in pig anterior pituitary cells. Pituitary cells from adult sows were treated for 4 or 24 h with rhleptin (from 0.1 nM to 1 microM) alone or in association with G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.2.8653
更新日期:2002-02-01 00:00:00
abstract::Axonal degeneration often leads to the death of neuronal cell bodies. Previous studies demonstrated the substantial protective role of 17β-estradiol (E2) in several types of neuron. However, most studies examined cell body protection, and the role of 17β-E2 in axonal degeneration of retinal ganglion cells (RGC) remain...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0046
更新日期:2011-07-01 00:00:00
abstract::Oxyntomodulin is derived from proglucagon processing in the intestine and the central nervous system. To date, no role in the central nervous system has been demonstrated. We report here that oxyntomodulin inhibits refeeding when injected intracerebroventricularly and into the hypothalamic paraventricular nucleus of 2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8430
更新日期:2001-10-01 00:00:00
abstract::Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1028
更新日期:1990-09-01 00:00:00
abstract::The maternal skeleton resorbs during lactation to provide calcium to milk and the lost mineral content is restored after weaning. The changes are particularly marked in Ctcgrp null mice, which lose 50% of spine mineral content during lactation but restore it fully. The known calciotropic hormones are not required for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1931
更新日期:2013-04-01 00:00:00