Abstract:
:Glial cell line-derived neurotrophic factor (GDNF) is expressed in many neuronal and non-neuronal tissues during development as well as in adult animals. GDNF signaling is mediated through a two-component system consisting of the so called GDNF receptor-alfa (GFRalpha1) which binds to GDNF. Thereafter this complex binds to and activates the tyrosine kinase receptor RET. In this work, for the first time, we have characterized the expression of both GDNF and RET in the anterior pituitary. First of all, RT-PCR analysis, Western blot and immunohistochemistry of the whole anterior pituitary showed that GDNF, GFRalpha1 and RET are expressed in this gland. Following double-immunofluorescence of consecutive sections we found GDNF immunoreactivity in most cell types, and it was most abundant in corticotrophs (55%), LH (59%) and FSH-producing cells (81%). In contrast, while the majority of somatotrophs (87%) were stained for RET, no positive immunostaining could be detected in other cell types. Taken together, this data indicate that gonadotrophs and corticotrophs are the main source of GDNF synthesized in the anterior pituitary and that the somatotrophs appears to be their target cell. This study provides direct morphological evidences that GDNF may well be acting in a paracrine-like fashion in the regulation of somatotroph cell growth and/or cell function.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Urbano AG,Suárez-Peñaranda JM,Diéguez C,Alvarez CVdoi
10.1210/endo.141.5.7548subject
Has Abstractpub_date
2000-05-01 00:00:00pages
1893-6issue
5eissn
0013-7227issn
1945-7170journal_volume
141pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Our research programs required the preparation of hypophysectomized and orchidectomized rhesus monkeys. This afforded us the possibility to characterize and compare levels of the gonadotropin and inhibin subunit mRNAs in pituitaries from intact and castrate monkeys. Eighteen adult male monkeys, four of which had been ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1537290
更新日期:1992-03-01 00:00:00
abstract::Somatostatin, also known as somatotropin release-inhibiting factor (SRIF), is secreted by pancreatic delta-cells and inhibits the secretion of both insulin and glucagon. SRIF initiates its actions by binding to a family of six G protein-coupled receptors (sst1, -2A, -2B, -3, -4, and -5) encoded by five genes. Messenge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6937
更新日期:1999-08-01 00:00:00
abstract::Although it is well known that chronic treatment with glucocorticoids inhibits somatic growth, the mechanism of action of this inhibitory effect is not completely understood. It is likely that glucocorticoids act at various levels, including pituitary, hypothalamus, and peripheral organs modulating GH synthesis, secre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940340
更新日期:1996-12-01 00:00:00
abstract::Vitellogenin (Vg) is a major yolk protein precursor in numerous oviparous animals. Numerous studies in bilateral oviparous animals have shown that Vg sequences are conserved across taxa and that Vgs are synthesized by somatic-cell lineages, transported to and accumulated in oocytes, and eventually used for supporting ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1086
更新日期:2013-09-01 00:00:00
abstract::UMR106-06 cells predominantly express the C1a isoform of the rat calcitonin (CT) receptor (CTR). We have compared the homologous regulation of the C1a CTR endogenously expressed in UMR106-06 cells with the cloned C1a CTR in transfected HEK 293 cells, in which expression is driven by a heterologous promoter. It was fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895320
更新日期:1996-11-01 00:00:00
abstract::COP9 signalosome subunit 5 (CSN5) plays a key role in carcinogenesis of multiple cancers and contributes to the stabilization of target proteins through deubiquitylation. However, the underlying role of CSN5 in thyroid carcinoma has not been reported. In this research, our data showed that CSN5 was overexpressed in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa206
更新日期:2021-03-01 00:00:00
abstract::The GnRH neurosecretory system undergoes marked structural and functional changes throughout life. The initial goal of this study was to examine the neuroanatomical relationship between GnRH neurons and a glycoprotein implicated in neuroplasticity, the polysialylated form of neural cell adhesion molecule (PSA-NCAM). U...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8000
更新日期:2001-03-01 00:00:00
abstract::Recent data suggest that proinflammatory cytokines secreted from adipose tissue contribute to the morbidity associated with obesity. However, characterization of the cell types involved in inflammation and how these cells promote insulin resistance in human adipocytes are unclear. We simulated acute inflammation using...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0536
更新日期:2006-11-01 00:00:00
abstract::Cripto-1 (TDGF1) is a multifunctional signaling factor that stimulates cellular effects, including proliferation, migration, survival, epithelial-to-mesenchymal transition, and angiogenesis, to regulate embryogenesis, tissue homeostasis, and tumorigenesis. Those cell behaviors are also associated with implantation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03039
更新日期:2018-04-01 00:00:00
abstract::Women with type 1 diabetes are subfertile. Diabetes negatively affects pregnancy by causing early miscarriage and poor prenatal outcomes. In this study we examine consequences of maternal type 1 diabetes on early embryo development, metabolic gene expression, and the pattern of insulin receptor (IR) and IGF-I receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0187
更新日期:2010-09-01 00:00:00
abstract::Acylation-stimulating protein (ASP) is a potent lipogenic protein produced by adipocytes. In vitro studies have shown that ASP increases triglyceride synthesis and glucose transport in both murine and human adipocytes. Our initial study indicated that complement C3-deficient (-/-) mice (and, therefore, ASP deficient) ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7364
更新日期:2000-03-01 00:00:00
abstract::Previous studies have shown that the adenylyl cyclase of intact granulosa cells from immature porcine follicles has a uniquely prolonged responsiveness to FSH and an atypically poor responsiveness to cholera toxin relative to other cells. The present studies were designed to determine if these characteristics were due...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-302
更新日期:1986-01-01 00:00:00
abstract::The regulatory role of cAMP-dependent protein kinase in steroidogenesis was examined in luteal cell mitochondria prepared from heavily luteinized prepubertal rat ovaries. The cAMP-dependent protein kinase, localized in luteal mitochondria, comprised 5.5% of the total cellular protein kinase activity (cAMP-dependent). ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-315
更新日期:1985-07-01 00:00:00
abstract::The xenoestrogen bisphenol A (BPA) has been shown to mimic estrogen both in vivo and in vitro. BPA stimulates PRL secretion and the expression of a PRL regulating factor from the posterior pituitary in the estrogen-sensitive Fischer 344 rat (F344), but not in Sprague-Dawley (SD) rats. The goal of the present studies w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6027
更新日期:1998-06-01 00:00:00
abstract::An estrogen-binding protein (EBP) has been identified and characterized in the cytosol of the pathogenic yeast Candida albicans. Binding of [3H]estradiol was found to be optimal at pH 7.4 in the presence of 0.3 M KCl and was linearly related to protein concentration. Binding was very rapid, reaching maximal levels in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-1965
更新日期:1989-04-01 00:00:00
abstract::Dispersed cells from whole testes or from isolated interstitial tissue of mature rats, yielded two distinct populations of Leydig cells when subjected to centrifugation in a 0-40% metrizamide gradient. One population (I) was found in a fractions with a density of 1.085-1.117 g/cm3, and the other population (II) was fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-5-1424
更新日期:1980-05-01 00:00:00
abstract::Telomerase activity is essential for protection of cells against the telomere erosion that occurs with each round of cell replication, and thus appears to play a role in the indefinite replication potential of some, but not all, eukaryotic cells. In this regard, some tissues contain stem cells that have a long prolife...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5839
更新日期:1998-03-01 00:00:00
abstract::Follistatin (FS), which binds to the inhibin/activin beta A- or beta B-subunit is localized with and modulates the biological actions of activin in many systems. However, in contrast to the wide distribution of the activin beta-subunit proteins and messenger RNAs (mRNA) in the brain, demonstration of FS mRNA signal ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612560
更新日期:1996-05-01 00:00:00
abstract::We have investigated the characteristics and properties of cholesterol esterase (CEase) in the rat adrenal to clarify the mechanism of synthesis of free cholesterol from esterified cholesterol. CEase activity was estimated using substrate vesicles which were sonicated mixtures of cholesteryl oleate and phosphatidylcho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-932
更新日期:1981-03-01 00:00:00
abstract::The monocarboxylate transporter Mct10 (Slc16a10; T-type amino acid transporter) facilitates the cellular transport of thyroid hormone (TH) and shows an overlapping expression with the well-established TH transporter Mct8. Because Mct8 deficiency is associated with distinct tissue-specific alterations in TH transport a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1800
更新日期:2014-01-01 00:00:00
abstract::Benign prostatic hyperplasia (BPH) is an age-related debilitating prostatic disease that is frequently associated with prostatic inflammation and bothersome lower urinary tract symptoms (LUTS). Animal models have shown that formalin- and bacterial-induced prostatic inflammation can induce bladder dysfunction; however,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa212
更新日期:2021-01-01 00:00:00
abstract::The effects of short term (1-month) and life-long 60% ad libitum food restriction on the adrenocortical response to restraint stress were compared in young and aged Fischer 344 rats. In rats restricted for 1 month (study 1), the adrenocortical response differed as a function of age. In 8-month-old animals, the initial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-4-1934
更新日期:1988-10-01 00:00:00
abstract::The adrenal zona glomerulosa (ZG) secretes aldosterone to regulate sodium balance. Chronic sodium restriction increases aldosterone accompanied by ZG expansion. The ZG is innervated by sympathetic, vasoactive intestinal polypeptide (VIP) and neuropeptide tyrosine (NPY), and sensory, calcitonin gene-related peptide, ne...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0487
更新日期:2010-10-01 00:00:00
abstract::Although polychlorinated biphenyls (PCBs) have been shown to accumulate in the adrenal, little is known about the effects of these endocrine disruptors on adrenal steroidogenesis. Our previous studies showed that high concentrations of PCB126 stimulated CYP11B1 and CYP11B2 mRNA expression and consequently raised corti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0823
更新日期:2006-03-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta) is a multifunctional growth factor in bone that is secreted as a latent complex and must be activated in order to influence cellular activity. We have investigated the influence of dexamethasone (Dex; a potent glucocorticoid) on the secretion and activation of latent TGF beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404670
更新日期:1993-11-01 00:00:00
abstract::Recent in vitro and in vivo evidence shows that secretion of parathyroid hormone (PTH) and calcitonin (CT) is stimulated by beta-adrenergic agonists and inhibited by beta-adrenergic antagonists. To assess the possible roles of adrenal medullary or adrenergic nerve terminal catecholamines in calcium homeostasis, we hav...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-4-977
更新日期:1980-10-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at a high level in the fetal pituitary and decreases profoundly between embryonic day 19 and postnatal day 1 (PN1), with a further decrease from PN1 to PN4. In this series of experiments, we investigated the hypothesis that dopamine 2 receptor (Dr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2147
更新日期:2014-07-01 00:00:00
abstract::Actions of protein products resulting from alternative splicing of the Igf1 gene have received increasing attention in recent years. However, the significance and functional relevance of these observations remain poorly defined. To address functions of IGF-I splice variants, we examined the impact of loss of IGF-IEa a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1279
更新日期:2011-05-01 00:00:00
abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyro...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2004-0959
更新日期:2004-12-01 00:00:00
abstract::To date, the role of pancreatic hormones in pancreatic islet growth and differentiation is poorly understood. To address this issue, we examined mice with a disruption in the gene encoding prohormone convertase 2 (PC2). These mice are unable to process proglucagon, prosomatostatin, and other neuroendocrine precursors ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0088
更新日期:2003-09-01 00:00:00