Abstract:
:Reduced cellular immune responses and altered cytokine production by cells from mice exposed to thermal injury are minimized if dehydroepiandrosterone (DHEA) is administered after experimental burn injury in mice. An analysis of similar tests of immune function developed by mice given the antiglucocorticoid, 17 beta-hydroxy-11 beta-[4-dimethylaminophenyl]17 alpha-propynyl-estra-4,5-diene-3-one (RU486), after the burn revealed no difference in immune function between the RU486-treated mice and the untreated burn group. At the levels of drug used, both DHEA and RU486 were able to completely block the effects of glucocorticoid treatment on immune function in mice, establishing a direct antiglucocorticoid activity of each steroid. Because thermal injury-mediated changes in immunity could be overcome by the administration of DHEA, but not RU486, the data suggest that the elevations in adrenal output of glucocorticoids are not responsible for the alterations in immune function after experimental thermal injury of mice. The results of this study have provided further insight into the mechanism of action of DHEA in this experimental model. The ability of DHEA to preserve immune function in severely thermally injured mice appears to extend beyond an antiglucocorticoid activity.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Araneo B,Daynes Rdoi
10.1210/endo.136.2.7835270subject
Has Abstractpub_date
1995-02-01 00:00:00pages
393-401issue
2eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Low basal GH secretion and reduced GH responsiveness to different GH secretagogues, including GHRF, have been reported in aged animals and humans. Parallel to the in vivo findings, an impaired GH responsiveness to GHRF is evident in somatotropes from old rats of either sex. We report here that in anterior pituitaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1649
更新日期:1987-11-01 00:00:00
abstract::Treatment of 5-day cyclic rats with 10 or 100 microgram estradiol benzoate on the day of estrus induced a luteal phase in all animals studied. On the other hand, an injection with 1 microgram estradiol benzoate given on the same day failed to induce pseudopregnancy. When 10 mg progesterone were injected on the day of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-1-195
更新日期:1979-07-01 00:00:00
abstract::SH2B1 is an SH2 domain-containing adaptor protein that plays a key role in the regulation of energy and glucose metabolism in both rodents and humans. Genetic deletion of SH2B1 in mice results in obesity and type 2 diabetes. Single-nucleotide polymorphisms in the SH2B1 loci and chromosomal deletions of the SH2B1 loci ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0254
更新日期:2010-08-01 00:00:00
abstract::For successful human placentation, invasion of trophoblast cells into the uterus and its associated vasculature is essential, and the regulation of this process is controlled by many factors at the fetal-maternal interface. N-acetylglucosaminyltransferase V (GnT-V) is a key enzyme that catalyzes beta1, 6-N-acetylgluco...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1005
更新日期:2009-02-01 00:00:00
abstract::We have recently identified, characterized, and cloned a luteal microsomal 32-kDa phosphoprotein that we named PRAP (for PRL-receptor associated protein), and we have demonstrated that PRAP binds to the intracellular domain of the short but not the long form of the PRL receptor. In this study, we used PRAP cDNA to exa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5336
更新日期:1997-08-01 00:00:00
abstract::The present studies were designed to evaluate the binding characteristics of arginine vasopressin (AVP), using rat anterior pituitary dispersed cells, in correlation with the biological activity of the peptide. Synthetic AVP released ACTH from dispersed anterior pituitary cells in a concentration-dependent fashion, wi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-6-2247
更新日期:1984-06-01 00:00:00
abstract::Two corticotropin-releasing factor 2 receptor (CRF2R)-selective peptides have been recently described, urocortin II (also known as stresscopin-related peptide) and urocortin III (stresscopin). We have used urocortin II to evaluate the effects of activation of the CRF2R on skeletal muscle-related physiological processe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0271
更新日期:2003-11-01 00:00:00
abstract::Slices of normal mammary tissue from pregnant and lactating mice and slices of neoplastic (MXT) mouse mammary tissue were superfused with estradiol (E2) and estrone (E1) each labeled with a different isotope. Isotope concentrations in tissue and perfusate at the steady state were used to calculate fractions and rates ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-399
更新日期:1983-07-01 00:00:00
abstract::An ovarian thecal/interstitial cell preparation was obtained by enzymatic digestion of immature rat ovaries depleted of granulosa cells. A component in thecal/interstitial cell-conditioned medium inhibited specific binding of [125I]iodo-epidermal growth factor ([125I]EGF) to its cell surface receptor, as determined wi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1892
更新日期:1987-11-01 00:00:00
abstract::Adipokines, cytokines mainly produced by adipocytes, are active participants in the regulation of inflammation. Administration of zymosan (ZY) was used to investigate the regulation and role of adipokines during peritonitis in mice. Injection of ZY led to a significant increase in leptin levels in both serum and perit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0327
更新日期:2008-08-01 00:00:00
abstract::The effect of constant infusions of bovine TSH in different concentrations on the pattern of T4 and T3 secretion was examined in perfused canine thyroids isolated in situ. Each of the thyroid lobes was isolated and perfused in each animal. It was thus possible to examine the effect of two different concentrations of T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-656
更新日期:1977-03-01 00:00:00
abstract::The development of androgen responsiveness in the submandibular gland of normal and androgen-resistant (Tfm) mice of different ages was studied after varying hormonal treatments. Total esteroproteolytic (tamase) activity of submandibular gland homogenates was used as a marker for androgen action. Newborn mice of all f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1571
更新日期:1975-06-01 00:00:00
abstract::We have examined the effects of a new synthetic inhibitor of mammalian tissue collagenase, CI-1 (N-[3-N-(benzyloxycarbonyl)amino-1-(R)carboxypropyl]L-leucyl-O-methyl-L- tyrosine N-methylamide; G. D. Searle SC 40827), and a general metalloproteinase inhibitor, 1,10-phenanthroline, on ovulation, as judged by the observa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1715
更新日期:1988-05-01 00:00:00
abstract::To examine developmental changes in the LH response to estrogen, eight neonatally ovariectomized monkeys received repeated injections (sc) of 50 micrograms/kg estradiol benzoate (EB) at approximately 4-month intervals starting at age 8-12 months and ending at 49-52 months. Serum samples were obtained 24 before and 0, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-6-2490
更新日期:1985-12-01 00:00:00
abstract::Daily treatment with 1 mg of the GnRH antagonist [Ac-delta 3-Pro1,pC1-D-Phe2,D-Trp3,6,N alpha MeLeu7]-GnRH abolished mating behavior and disrupted spermatogenesis in male rats within 2 weeks. This was accompanied by a sharp decrease in plasma testosterone (T) levels as well as sexual organ weights while body weight re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1998
更新日期:1981-05-01 00:00:00
abstract::We have demonstrated two heat shock proteins (HSP's) in the human placenta that specifically bind calcitonin. Binding specificity was shown by ligand-affinity chromatography and by competitive binding studies. The HSP's were identified by Western analysis and by amino acid sequence. Our observations are consistent wit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-672
更新日期:1990-01-01 00:00:00
abstract::We have investigated the interaction of estrogen with GnRH on the regulation of LH subunit mRNA in female hypogonadal (hpg) mice receiving constant frequency and amplitude pulsatile GnRH treatment for up to 18 days. The level of cytosolic common alpha mRNA in female hpg mouse pituitaries was 45 +/- 6% of normal female...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-1744
更新日期:1989-04-01 00:00:00
abstract::[125I]-[D-Ser(TBU)6]LHRH-EA binds to a single class of high affinity sites in rat anterior pituitary cells in culture at an apparent dissociation constant of 0.25 nM at 0-4C. The order of potency of a representative group of LHRH agonists and antagonists to displace the labeled ligand is similar to their LH-releasing ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-350
更新日期:1981-01-01 00:00:00
abstract::We studied the effects of 17 beta-estradiol (E2) and PTH on alkaline phosphatase activity in SaOS-2 cells that had been passaged and grown continually in the presence (SaOS + Dex) or absence (SaOS - Dex) of 10(-8) M dexamethasone (Dex). We showed that the more differentiated SaOS + Dex cells had higher alkaline phosph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299560
更新日期:1994-02-01 00:00:00
abstract::Specific serum binding of 25-hydroxy-cholecalciferol (25-OHD3) was measured by saturation analysis in rats of various ages, and during vitamin D deprivation. The serum binding capacity for 25-OHD3 was observed to increase until age 6-8 weeks, then decline and remain stable thereafter at 3.7 x 10(-6) M. The 25-hydroxyv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-642
更新日期:1977-03-01 00:00:00
abstract::The possibility of direct pituitary effects of sex steroids on gonadotropin gene expression and synthesis was studied in male rats. The animals were treated with a potent GnRH antagonist, Ac-D-pClPhe-D-pClPhe-D-Trp-Ser-Tyr-D-Arg-Leu-Arg-Pro-D-Ala-+ ++NH2CH3COOH (Org 30276; 0.5 mg/kg BW, sc, twice daily) for 10 days. G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-3204
更新日期:1990-06-01 00:00:00
abstract::To study conversion of 3,5,3'-triiodothyroinine (T3) and 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (T2) in vitro, T3 or rT3 was incubated at pH 7.35 with homogenates of several rat tissues (liver, kidney, muscle, heart, ling, spleen, intestines, and brain) for 15 min at 37 C. The T2 generated during incub...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-4-1099
更新日期:1978-04-01 00:00:00
abstract::The ability of FSH to stimulate the activity of ornithine decarboxylase (ODC) in the ovary of the immature rat and cycling hamster has been examined using specific antisera to gonadotropins. The stimulatory effect of FSH on ODC activity in the ovary of the immature rat was abolished when LH antiserum was administered ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-5-1480
更新日期:1979-05-01 00:00:00
abstract::Homogenates from male ddy mouse sublingual glands were incubated with 100 nM [125I]rT3 in the presence of 2 mM dithiothreitol (DTT). Metabolites were analyzed by HPLC or Dowex-50 minicolumn assay. 3,3'-Diiodothyronine and I- were the only appreciable products. Iodide release from rT3 was compatible with the enzymatic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.8440180
更新日期:1993-03-01 00:00:00
abstract::A detailed understanding of the mechanism of action of estrogen on bones is still lacking. The present study was designed to examine possible modulation by 17 beta-estradiol (E2) on the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and on vitamin D receptors (VDR) in the ROS 17/2.8 osteoblast-like cell line. Cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315250
更新日期:1992-05-01 00:00:00
abstract::Fasting increases hypothalamic neuropeptide Y (NPY) and agouti-related peptide (AGRP) messenger RNA (mRNA) and reduces hypothalamic POMC mRNA, and is also characterized by a reduction in plasma leptin, insulin, and glucose, each of which has been implicated in the regulation of hypothalamic gene expression. To further...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.6966
更新日期:1999-10-01 00:00:00
abstract::Obesity induced by high-fat (HF) feeding is associated with low-grade inflammation in peripheral tissues that predisposes to insulin resistance. Recent evidence suggests the occurrence of a similar process in the hypothalamus, which favors weight gain through impairment of leptin and insulin signaling. In addition to ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2010-0336
更新日期:2010-09-01 00:00:00
abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588270
更新日期:1995-12-01 00:00:00
abstract::Oxytocin (OT) is a hypothalamic nonapeptide that is synthesized as part of a larger precursor protein that also contains an approximately 10-kDa protein called neurophysin at its C-terminus. This precursor protein is trafficked through the regulated secretory pathway into secretory granules and then axonally transport...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8700
更新日期:2002-03-01 00:00:00
abstract::Bilateral destruction of the hypothalamic paraventricular nuclei (PVN) produced a profound depression of plasma TSH and the median eminence TRH concentration in hypothyroid rats. Anterior pituitary type II iodothyronine 5'-deiodinase (5'-D) activity was consistently lower but not significantly different in sham- and P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1676
更新日期:1988-09-01 00:00:00