Androgens decrease LHRH binding sites in rat anterior pituitary cells in culture.

Abstract:

:[125I]-[D-Ser(TBU)6]LHRH-EA binds to a single class of high affinity sites in rat anterior pituitary cells in culture at an apparent dissociation constant of 0.25 nM at 0-4C. The order of potency of a representative group of LHRH agonists and antagonists to displace the labeled ligand is similar to their LH-releasing activity. Treatment of pituitary cells for 48 h with 100 nM 5 alpha-dihydrotestosterone leads to a 40% decrease of the number of LHRH receptors with no change of binding affinity. This loss of LHRH receptors is accompanied by a similar decrease of the LH responsiveness to LHRH, thus providing the first evidence for a direct effect of sex steroids on pituitary LHRH receptors as a possible mechanism of feedback action.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Giguere V,Lefebvre FA,Labrie F

doi

10.1210/endo-108-1-350

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

350-2

issue

1

eissn

0013-7227

issn

1945-7170

journal_volume

108

pub_type

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