Abstract:
:[125I]-[D-Ser(TBU)6]LHRH-EA binds to a single class of high affinity sites in rat anterior pituitary cells in culture at an apparent dissociation constant of 0.25 nM at 0-4C. The order of potency of a representative group of LHRH agonists and antagonists to displace the labeled ligand is similar to their LH-releasing activity. Treatment of pituitary cells for 48 h with 100 nM 5 alpha-dihydrotestosterone leads to a 40% decrease of the number of LHRH receptors with no change of binding affinity. This loss of LHRH receptors is accompanied by a similar decrease of the LH responsiveness to LHRH, thus providing the first evidence for a direct effect of sex steroids on pituitary LHRH receptors as a possible mechanism of feedback action.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Giguere V,Lefebvre FA,Labrie Fdoi
10.1210/endo-108-1-350subject
Has Abstractpub_date
1981-01-01 00:00:00pages
350-2issue
1eissn
0013-7227issn
1945-7170journal_volume
108pub_type
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