Abstract:
:VEGF is produced by osteoblasts and has been postulated to function as an angiogenic stimulus during normal skeletal development and in fracture repair. In this study, we characterized the molecular mechanisms by which experimental hypoxia increases VEGF mRNA in human MG63 osteoblast-like cells. Exposure of MG63 cells to 1% O(2) for 24 h resulted in a four-fold increase in VEGF mRNA. Immunoblotting of nuclear extracts demonstrated a time-dependent increase in the level of the Hif-2alpha protein, which preceded the rise in VEGF mRNA. To determine the effect of hypoxia on VEGF gene transcription, MG63 cells were transiently transfected with a segment of the VEGF promoter construct fused to luciferase and then exposed to 1% O(2). Hypoxia induced VEGF promoter activity five-fold by 24 h. Forced expression of Hif-2alpha, but not Hif-1alpha, increased both basal and hypoxia induced VEGF promoter activity. By contrast, the ability of the VEGF reporter to respond to hypoxia or recombinant Hif-2alpha was abolished in cells transfected with a VEGF promoter construct containing a mutation in the hypoxia response element. In summary, exposure of osteoblast-like cells to hypoxia induces VEGF expression via induction of Hif-2alpha and transcriptional activation of the VEGF promoter.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Akeno N,Czyzyk-Krzeska MF,Gross TS,Clemens TLdoi
10.1210/endo.142.2.8112subject
Has Abstractpub_date
2001-02-01 00:00:00pages
959-62issue
2eissn
0013-7227issn
1945-7170journal_volume
142pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Although GH plays a key role in postnatal growth, prenatal growth is thought to be GH independent. However, recent data has shown GH receptor/binding protein (GHR/BP) to be present in rat fetal tissues as early as fetal stage E12. The aim of the present study was to investigate tissue-specific production of the GHR/BP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664680
更新日期:1995-10-01 00:00:00
abstract::The present study was undertaken to investigate the role of angiotensin II (Ang II) in ovulation and ovarian steroidogenesis and prostaglandin (PG) production via the Ang II receptors in rabbit ovaries. In in vitro perfused rabbit ovaries, PD123319, a selective nonpeptide antagonist for AT2 receptors, reduced the Ang ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.4.8625890
更新日期:1996-04-01 00:00:00
abstract::A new perfusion system of isolated rat femora was established, and the effect of PTH or calcitonin (CT) on cAMP release from the adult bone was examined. Stimulation of cAMP release from the isolated perfused bone peaked rapidly between 5 and 10 min after human PTH (hPTH)-(1-34) or eel CT injection, respectively, and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1901
更新日期:1985-11-01 00:00:00
abstract::With a recently developed microdissection technique, four circumventricular organs were removed from the rat brain, and their contents of LHRH and TRH were measured. The subfornical organ, the organon vasculosum lamia terminalis, the subcommissural organ, the area postrema all contained significant quantities of both ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-2-311
更新日期:1976-02-01 00:00:00
abstract::Insulin and epidermal growth factor (EGF) share a number of metabolic actions, including stimulation of protein synthesis and growth in certain tissues, and activation of apparent receptor tyrosine kinase activities. We have shown that insulin and EGF promote the phosphorylation of a number of intracellular proteins i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-141
更新日期:1984-01-01 00:00:00
abstract::The effects of electrical stimulation of the brain on the ovarian venous plasma concentrations of estradiol (E2) and progesterone (P) were investigated in female rats hypophysectomized and adrenalectomized at 1000 h on the day of proestrus. Stimulation was applied during the proestrous critical period under pentobarbi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-136
更新日期:1981-07-01 00:00:00
abstract::In this study we tested the hypothesis that receptor-mediated transport of urocortin across the blood-brain barrier (BBB) undergoes developmental changes. Urocortin is a peptide produced by both selective brain regions and peripheral organs, and it is involved in feeding, memory, mood, cardiovascular functions, and im...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1039
更新日期:2010-03-01 00:00:00
abstract::IGF binding protein-2 (IGFBP-2) has been implicated in the development and spread of a number of tumor types, and its abrogation in experimental models of cancer is associated with decreased tumor growth. This suggests that targeted inhibition of IGFBP-2 expression in some cancers may have therapeutic benefit. In this...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1335
更新日期:2007-05-01 00:00:00
abstract::The localization of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the adrenal gland of the frog, Rana ridibunda, was examined by the indirect immunofluorescence technique. Using an antiserum directed against rat alpha-CGRP, the presence of a network of positive fibers was observed in the adrenal pare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013380
更新日期:1994-07-01 00:00:00
abstract::The involvement of IGF-I in mammary carcinogenesis is well established, but the role of GH, as an autocrine growth factor for breast cancers is poorly understood. The goal of our study was to investigate whether antagonists of GHRH can interfere with the effects of GH and IGF-I in MXT mouse mammary cancers. GHRH antag...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8426
更新日期:2001-10-01 00:00:00
abstract::In the present work we describe homologous desensitization of gonadotropin release from pituitary cells in response to short-term exposure to GnRH. Like the release process itself, desensitization requires receptor occupancy by an agonist. In contrast, however, inhibition of LH release by chelation of extracellular ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-1-408
更新日期:1983-01-01 00:00:00
abstract::The effect of a potent agonistic LHRH analog D-Trp-6-LHRH on the hyperprolactinemia induced by haloperidol was tested in intact and ovariectomized female rats. The administration of D-Trp-6-LHRH at two dose levels (5 and 50 micrograms/day) for 20 days blocked the increase in serum PRL induced by haloperidol in intact ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2227
更新日期:1985-06-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of the u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00396
更新日期:2017-11-01 00:00:00
abstract::The Leydig cells of the testis are known to possess high affinity receptors for luteinizing hormone and human chorionic gonadotropin (hCG), but no information concerning the synthesis of these receptors is available yet. In order to investigate this question, we have purified crude rat interstitial cell preparations o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2129
更新日期:1982-12-01 00:00:00
abstract::Active immunization of male rabbits results in a marked increase in plasma testosterone (T) and in the fraction of T bound to circulating proteins. In order to identify the cause of the increased T levels, the half-life of T after castration, and the metabolic clearance rate (MCR) and production rate (PR) after a sing...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-5-1142
更新日期:1976-05-01 00:00:00
abstract::Recent evidence suggests that growth hormone-releasing peptides (GHRPs) mimic an unidentified native GH-releasing hormone (GHRH)-amplifying hormone. GHRH has been shown to stimulate food intake acting on the central nervous system. The present studies were conducted to test the hypothesis that GHRPs may also potentiat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895390
更新日期:1996-11-01 00:00:00
abstract::This study aimed to delineate the hypothalamic and/or pituitary actions of testosterone and its primary metabolites 5 alpha-dihydrotestosterone and estradiol (E) in adult castrated rams (wethers) during the breeding season. In Exp 1, wethers were treated for a week with twice daily injections (im) of peanut oil, 8, 16...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-6-3080
更新日期:1991-12-01 00:00:00
abstract::Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains cha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0341
更新日期:2010-09-01 00:00:00
abstract::Thyroid hormones play a major role in body homeostasis, regulating energy expenditure and cardiovascular function. Given that obese people or athletes might consider rapid weight loss as beneficial, voluntary intoxication with T4 preparations is a growing cause for thyrotoxicosis. However, the long-lasting effects of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1095
更新日期:2016-07-01 00:00:00
abstract::The hypoxic growth plate cartilage requires hypoxia-inducible factor (HIF)-mediated pathways to maintain chondrocyte survival and differentiation. HIF proteins are tightly regulated by prolyl hydroxylase domain-containing protein 2 (Phd2)-mediated proteosomal degradation. We conditionally disrupted the Phd2 gene in ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1473
更新日期:2016-01-01 00:00:00
abstract::In the pituitary, GABA regulates the release of several hormones via different receptors. GABA(C) receptors are heterooligomers that differ from GABA(A) receptors in that they contain p-subunits and are insensitive to bicuculline. However, molecular and functional evidence for the presence of GABA(C) receptors outside...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7476
更新日期:2000-05-01 00:00:00
abstract::We sequenced the complementary DNA (cDNA) encoding guinea pig LHRH from an expression library derived from the preoptic area-anterior hypothalamus. Data from in situ hybridization and RNase protection assays verified that the cloned cDNA was complementary to guinea pig LHRH messenger RNA. The architecture of the deduc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5454
更新日期:1997-10-01 00:00:00
abstract::To determine if the inhibitory effects of glucocorticoids on GnRH-stimulated secretion of LH observed in male rats in vivo are exerted directly on the pituitary, dispersed pituitary cells from adult male rats were treated with 60 or 600 ng/ml cortisol (F) or corticosterone (B) during one or two 48-h incubations. Contr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-855
更新日期:1985-09-01 00:00:00
abstract::The mechanism by which the proto-oncogene, c-fos, is up-regulated in response to PGE2 in the mouse osteoblastic (MC3T3-E1) cell line was investigated using RT-PCR. c-fos messenger RNA up-regulation by dmPGE2 is rapid, starting 10 min post stimulation, and transient. The specific protein kinase A (PKA) inhibitor, H89, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7271
更新日期:2000-01-01 00:00:00
abstract::Thyroid hormones (TH) cause an increase in spontaneous electrical activity of cultured rat skeletal myotubes. This activity is associated with tetrodotoxin (TTX)-sensitive Na channels. In addition, the initial effect of TH on Na-K pump synthesis has been shown to be TTX dependent. Accordingly, we have studied effects ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-842
更新日期:1989-08-01 00:00:00
abstract::Chronic exposure to stressors impairs the function of multiple organ systems and has been implicated in increased disease risk. In the rodent, the chronic variable stress (CVS) paradigm has successfully modeled several stress-related illnesses. Despite striking disparities between men and women in the prevalence and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00217
更新日期:2018-07-01 00:00:00
abstract::Elevated activin A levels in inhibin-deficient mice promote the development of gonadal tumors and induce cachexia by reducing muscle, liver, stomach, and fat mass. Because activin A is an important regulator of tissue growth, inhibiting the actions of this TGFβ family ligand may halt or reverse pathology in diseased t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1052
更新日期:2011-10-01 00:00:00
abstract::Vitamin D receptor (VDR) null mice develop hypocalcemia, hyperparathyroidism, rickets, osteomalacia and alopecia. Normalization of mineral ion homeostasis prevents all of these abnormalities except alopecia. Hair reconstitution assays, performed in athymic nude mice, demonstrate that the lack of VDR in keratinocytes l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8650
更新日期:2001-12-01 00:00:00
abstract::L-Asparaginase (ASNase), a drug widely used in the treatment of acute lymphoblastic leukemia, has been reported to decrease serum T4-binding globulin (TBG) levels, while results of serum albumin determinations were conflicting. This effect in vivo has been attributed to depressed liver protein synthesis, but this hypo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-3-1185
更新日期:1986-09-01 00:00:00