Abstract:
:The effects of secretagogue(s) from mouse decidual tissue on the release of mouse placental lactogen-II (mPL-II) were studied. Decidual tissue was obtained from 10- and 11-day-pregnant mice. The tissue was homogenized, extracted, and the tissue extract was made 50% saturated with ammonium sulfate. Both the precipitate and supernatant were tested for their ability to stimulate mPL-II release from cultured trophoblasts. The supernatant contained an activity to stimulate the release of mPL-II. This activity was further purified using column chromatography. The purification resulted in isolation of a protein with a mol wt of 20 K as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions and 6 K under reducing conditions. Further characterization of this protein showed that it binds calcium and has an amino acid sequence that is highly homologous with calcyclin expressed in mouse embryonic fibroblast cells and with calcyclin from other species. This protein was designated mouse decidual calcyclin. Antiserum was raised against the purified decidual calcyclin for development of an RIA and for immunoblots. Western blots of various mouse tissue extracts and mouse serum from different physiological stages showed that the concentration of calcyclin was highest in decidual tissue. Detectable levels were found in extracts from trophoblast, lung, and stomach, but the concentrations in these tissues were about 100 times lower than in decidua. Decidual calcyclin was not detectable in mouse serum. Cultured decidual cells released calcyclin into the medium. On average, this release was about 7.8 ng/micrograms DNA.24 h. The rate of release did not change significantly during 4 days of culture. The ratio of calcyclin in cells per calcyclin released during 24 h averaged 2.3 and did not change significantly during the culture period. The purified decidual calcyclin stimulated the release of mPL-II from cultured trophoblasts in a dose-dependent manner at concentrations from 0.01 to 1 microgram/ml. The maximum stimulation averaged about 1.5 times above control. It is concluded that decidual calcyclin may be of physiological importance for the regulation of mPL-II secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Thordarson G,Southard JN,Talamantes Fdoi
10.1210/endo-129-3-1257subject
Has Abstractpub_date
1991-09-01 00:00:00pages
1257-65issue
3eissn
0013-7227issn
1945-7170journal_volume
129pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The maximum amounts of angiotensins II and III that were confined to the kidney during intrarenal arterial administration in conscious dogs were determined to be 2 and 12 pmol/kg X min, respectively. These doses were infused chronically intrarenally to uninephrectomized dogs. A significant reduction in urinary volume ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-2160
更新日期:1985-11-01 00:00:00
abstract::The tissue-specific differential processing of proglucagon (Pg) yields glucagon in pancreatic A cells and glucagon-like peptide-1 (GLP-1), GLP-2, and glicentin in intestinal L cells. It has been suggested that the difference in Pg cleavage in A and L cells is due to the presence of distinct prohormone convertases (PC)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7068
更新日期:1999-10-01 00:00:00
abstract::PTH-related protein (PTHrP) and its receptor are expressed in vascular smooth muscle cells and are believed to participate in the local regulation of vascular tone. To explore the function of locally produced PTHrP in vascular smooth muscle in vivo, we developed transgenic mice that overexpress PTHrP in smooth muscle ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6646
更新日期:1999-04-01 00:00:00
abstract::We have recently reported that a subpopulation of galanin (GAL)-immunoreactive perikarya in the preoptic area near the organum vasculosum of the lamina terminalis (OVLT) has morphological characteristics similar to those of LHRH-containing neurons. In fact, both peptides are colocalized in those neurons in the male ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1977
更新日期:1991-10-01 00:00:00
abstract::Antisera against proopiomelanocortin (POMC)-derived peptides have previously been employed to demonstrate immunostainable materials in the male reproductive tract and in the corpus luteum of rat ovary. The present study was designed to determine how the distribution of such stainable materials varies in mouse ovary as...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-378
更新日期:1984-07-01 00:00:00
abstract::GRK2 is a member of the G protein-coupled receptor kinase family that phosphorylates the activated form of beta-adrenergic and other G protein-coupled receptors and plays an important role in their desensitization and modulation. Alterations in thyroid hormone levels have been reported to lead to important changes in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8039
更新日期:2001-03-01 00:00:00
abstract::A detailed understanding of the mechanism of action of estrogen on bones is still lacking. The present study was designed to examine possible modulation by 17 beta-estradiol (E2) on the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and on vitamin D receptors (VDR) in the ROS 17/2.8 osteoblast-like cell line. Cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315250
更新日期:1992-05-01 00:00:00
abstract::GH secretion is markedly blunted in obesity; however, the mechanism(s) mediating this response remains to be elucidated. In the present study we examined the involvement of the two hypothalamic GH-regulatory hormones, GH-releasing factor (GRF) and somatostatin (SRIF), using the genetically obese male Zucker rat. Spont...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-6-3087
更新日期:1990-12-01 00:00:00
abstract::Oxytocin (OT) is released by the posterior pituitary during male orgasm and is supposed to participate in the ejaculatory process. We now report evidence demonstrating the presence of an OT receptor gene (real-time RT-PCR and Northern blot) and protein (immunohistochemistry, Western blot, and binding studies) expressi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0962
更新日期:2004-04-01 00:00:00
abstract::We have used immunohistochemistry to define the cellular distribution of GH receptors in the gastrointestinal tract (GIT) and its derivatives. Immunohistochemistry was performed in the adult rat GIT with a panel of characterized monoclonal antibodies to the GH receptor. The most intense and heterogeneous immunoreactiv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-299
更新日期:1990-01-01 00:00:00
abstract::Somatostatin (SRIH) inhibits cell proliferation by interacting with five distinct SRIH receptor subtypes (SSTRs) activating several pathways in many tissues. We previously demonstrated that SRIH, by activating Src homology-2-containing protein, inhibits cell proliferation of the human medullary thyroid carcinoma cell ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1479
更新日期:2006-07-01 00:00:00
abstract::QRFP, an RFamide peptide, was recently identified as an endogenous ligand of an orphan G protein-coupled receptor, GPR103. Recent investigation revealed that acute intracerebroventricular (ICV) administration of QRFP26/P518/26RFa, a constitutive part of QRFP43 (43-amino acid-residue form of QRFP), increases appetite i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1580
更新日期:2006-06-01 00:00:00
abstract::In an effort to define biochemical events relevant to the adipogenic action of GH, the effect of GH on expression of the cytoskeletal element vinculin was studied in 3T3-F442A preadipose cells. Results from Western blotting indicated that in serum-free medium 2 nM met-hGH induced an approximately 100% increase in vinc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-1-527
更新日期:1991-07-01 00:00:00
abstract::The steroid sex hormone progesterone (P) induces the expression of a variety of genes through a signal transduction pathway mediated by the P receptor, a DNA-binding regulator of transcription. To identify genes and gene networks that are P dependent in the rhesus endometrium, we used a powerful technique employing su...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119170
更新日期:1994-03-01 00:00:00
abstract::The circadian rhythm of glucocorticoids affects diverse physiological systems, including stress responses and the coordination of rhythmic functions in peripheral and central tissues. Circadian clocks are considered to be important coordinators of glucocorticoid release and loss of the core clock component Brain and m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1330
更新日期:2016-11-01 00:00:00
abstract::LA-PTH is a long-acting parathyroid hormone (PTH) peptide analogue in preclinical development for hypoparathyroidism (HP). Like native PTH, LA-PTH contains a methionine at position 8 (Met8) that is predicted to be critical for function. We assessed the impact of Met oxidation on the functional properties of LA-PTH and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa216
更新日期:2021-02-01 00:00:00
abstract::An isolated perfused rabbit ovary preparation was used to determine the effects of cyanoketone, a potent inhibitor of 3 beta-hydroxysteroid dehydrogenase, on ovulation, ovum maturation and fertilizability, and steroid production. In the first experiment, cyanoketone (10(-4) M) was added to the perfusate of one ovary. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2555
更新日期:1987-06-01 00:00:00
abstract::Estrogens have been implicated in the regulation of prolactin gene expression in man, although previous studies have not defined the molecular mechanism whereby estradiol activates the human prolactin gene promoter (hPrl). We found that estradiol induced a reproducible 1.8-fold activation of the hPrl gene promoter, us...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1066
更新日期:2008-02-01 00:00:00
abstract::Glutamate (Glu) is the most prevalent excitatory neurotransmitter in the brain and has been implicated in the regulation of GnRH secretion in several mammalian species, including the monkey. To investigate the neuroanatomical basis for Glu-GnRH interactions, we performed an immunocytochemical study at both the light a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7905410
更新日期:1994-02-01 00:00:00
abstract::Islet β-cell dysfunction that leads to impaired insulin secretion is a principal source of pathology of diabetes. In type 2 diabetes, this breakdown in β-cell health is associated with compromised islet-enriched transcription factor (TF) activity that disrupts gene expression programs essential for cell function and i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa213
更新日期:2021-02-01 00:00:00
abstract::PRL receptor (PRL-R) expression has been analyzed in human hematopoietic tissues using flow cytofluorometric analysis with a series of biotinylated monoclonal antibodies (mAbs) directed against the extracellular domain of the rat liver PRL-R. In the thymus, more than 75% of cells were labeled by the anti-PRL-R mAb. Re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156910
更新日期:1994-05-01 00:00:00
abstract::An estrogen-binding protein (EBP) has been identified and characterized in the cytosol of the pathogenic yeast Candida albicans. Binding of [3H]estradiol was found to be optimal at pH 7.4 in the presence of 0.3 M KCl and was linearly related to protein concentration. Binding was very rapid, reaching maximal levels in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-4-1965
更新日期:1989-04-01 00:00:00
abstract::Parathyroid hormone (PTH) secretion rate was measured in seven calves by using a technique which involved RIA of parathyroid venous blood collected during timed intervals and measured volumetrically. Infusion of solutions of NA2EDTA and MgCl2 into the jugular vein was used to alter plasma calcium and magnesium concent...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-6-1803
更新日期:1978-06-01 00:00:00
abstract::Chronically elevated circulating glucocorticoid levels are although to enhance vulnerability to psychopathology. Here we hypothesized that such sustained glucocorticoid levels, disturbing corticosterone pulsatility, attenuate glucocorticoid receptor signaling and target gene responsiveness to an acute challenge in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1119
更新日期:2010-03-01 00:00:00
abstract::Female and male brains differ. Differences begin early during development due to a combination of genetic and hormonal events and continue throughout the lifespan of an individual. Although researchers from a myriad of disciplines are beginning to appreciate the importance of considering sex differences in the design ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2004-1142
更新日期:2005-04-01 00:00:00
abstract::Although many studies have investigated the complex interrelationships between thyroid hormone levels and insulin-glucose secretion and action, there is at present a lack of information concerning the effects of various thyroid hormone levels on insulin receptors. In the present study, rat liver membranes were prepare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-1922
更新日期:1982-06-01 00:00:00
abstract::Pure porcine cholecystokinin-33 [the triacontatriapeptide form of cholecystokinin (CCK-33)], gastric inhibitory polypeptide (GIP), and secretin were infused in rats in doses of 1, 10 and 1000 pmol/kg . min. The peptides were administered alone or in combination with glucose (40 mg/kg . min) or arginine (50 mg/kg . min...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1268
更新日期:1982-04-01 00:00:00
abstract::Testerone (T) concentrations in the plasma, gonads, and adrenals were measured by radioimmunoassay in 188 male and 160 female rabbit fetuses. Determinations were performed daily from the 20th to the 31st day of gestation and were correlated with the maternal plasma concentration of T. The T content of both testes rema...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-5-1263
更新日期:1976-11-01 00:00:00
abstract::Although tamoxifen (TAM), which is widely used in the treatment of breast cancer, also has a beneficial effect on cisplatin-refractory ovarian cancer, the biological mechanism of this effect has remained obscure. TAM, besides its action as an antiestrogen, also inhibits cell proliferation of estrogen receptor (ER)-neg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0709
更新日期:2004-03-01 00:00:00
abstract::As we previously showed, we have synthesized a new family of 17β-estradiol-platinum(II) hybrids. Earlier studies revealed the VP-128 hybrid to show high efficiency compared with cisplatin toward hormone-dependent breast cancer cells. In the present research, we have studied the antitumor activity of VP-128 in vitro an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1083
更新日期:2013-07-01 00:00:00