Abstract:
:Oxytocin (OT) is released by the posterior pituitary during male orgasm and is supposed to participate in the ejaculatory process. We now report evidence demonstrating the presence of an OT receptor gene (real-time RT-PCR and Northern blot) and protein (immunohistochemistry, Western blot, and binding studies) expression in the rabbit and human corpus cavernosum (CC) and its possible involvement in postorgasmic penile detumescence. OT receptor is expressed in the penis at a concentration similar to that present in other portions of the male genital tract and mediates CC contractility. OT-induced CC contractility is clearly regulated by the changing sex steroid milieu. In fact, we found that in a rabbit model of hypogonadotropic hypogonadism (induced by a single administration of the long-acting GnRH agonist triptorelin pamoate, 2.9 mg/kg), OT responsiveness was strongly reduced and was completely restored by estradiol valerate (3.3 mg/kg weekly), but not by testosterone enanthate (30 mg/kg weekly). As we found that CC expresses both subtypes of estrogen receptors and P450 aromatase, we hypothesized a physiological role for endogenous estrogens in regulating OT responsiveness. We therefore treated adult rabbits with an aromatase inhibitor (letrozole, 2.5 mg/kg) or an antiestrogen (tamoxifen, 0.25 mg/kg) for 3 wk. Both treatments significantly reduced CC responsiveness to OT stimulation. In conclusion, these findings indicate that OT might participate in inducing postorgasmic penile flaccidity and suggest a new role for estrogens in the male: regulation of CC responsiveness to OT.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Vignozzi L,Filippi S,Luconi M,Morelli A,Mancina R,Marini M,Vannelli GB,Granchi S,Orlando C,Gelmini S,Ledda F,Forti G,Maggi Mdoi
10.1210/en.2003-0962subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1823-34issue
4eissn
0013-7227issn
1945-7170pii
en.2003-0962journal_volume
145pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Testicular cytosol from Sprague-Dawley rats was shown to possess an estradiol binding component which exhibited the properties of a receptor. Specific binding of estradiol by the receptor was shown, at 4C in vitro, to reach equilibrium by 6 h. That level of binding was maintained at a plateau for 18 h. Estradiol bindi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-2-555
更新日期:1976-08-01 00:00:00
abstract::The GT1 GnRH cell lines express all three subunits of the cyclic nucleotide-gated (CNG) channels (CNG2, 4.3 and 5) expressed in olfactory neurons. We investigated using in situ hybridization and double immunofluorescence whether endogenous GnRH neurons in therat also express CNG channel subunits. Sections from male an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8951
更新日期:2002-06-01 00:00:00
abstract::We have recently reported evidence for the presence of stereospecific energy-dependent transport processes for T3 in rat tissues. These processes were responsible for maintenance of concentration differences of free L- and D-T3 across the cellular plasma and nuclear membranes. In rat liver, the free L-T3 concentration...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-6-2449
更新日期:1985-12-01 00:00:00
abstract::The pineal hormone melatonin regulates reproductive function in seasonally breeding mammals. Recent studies using 125I-labeled 2-iodomelatonin (I-MEL) reveal that the distribution of putative melatonin receptors is species-specific; only the hypophysial pars tuberalis (PT) is a consistent site of I-MEL binding in all ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2607
更新日期:1990-11-01 00:00:00
abstract::We have previously shown that plasma IGF 1 concentration is positively correlated with the rate of antler growth and have proposed that IGF 1 is stimulatory for antler growth in the red deer stag. Therefore to partly resolve the question of whether the IGF 1 was of circulating or local origin in relation to its effect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-3005
更新日期:1988-06-01 00:00:00
abstract::IGF binding proteins-3 and -4, whether given in the perfused rat heart or given iv in the intact animal, cross the microvascular endothelium of the heart and distribute in subendothelial tissues. IGF binding protein-3, like IGF-I/II, localizes in cardiac muscle, with lesser concentrations in CT elements. In contrast, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8353
更新日期:2001-09-01 00:00:00
abstract::Exposure of quiescent bovine parathyroid cells to serum or a serum substitute caused an elevation in [3H] thymidine incorporation, followed by an increase in cell number. This was preceded by a rapid transient rise in c-myc and c-fos proto-oncogene mRNA levels. Alterations in the medium calcium concentration had no ef...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-935
更新日期:1989-08-01 00:00:00
abstract::Our research programs required the preparation of hypophysectomized and orchidectomized rhesus monkeys. This afforded us the possibility to characterize and compare levels of the gonadotropin and inhibin subunit mRNAs in pituitaries from intact and castrate monkeys. Eighteen adult male monkeys, four of which had been ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1537290
更新日期:1992-03-01 00:00:00
abstract::Trophoblast hypoxia and injury, key components of placental dysfunction, are associated with fetal growth restriction and other complications of pregnancy. Accumulation of lipid droplets has been found in hypoxic nonplacental cells. Unique to pregnancy, lipid accumulation in the placenta might perturb lipid transport ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00752
更新日期:2018-12-01 00:00:00
abstract::By acting in the brain, insulin suppresses food intake, whereas neuropeptide Y (NPY) has the opposite effect. Since fasting increases NPY gene expression in the hypothalamic arcuate nucleus (ARC) and also lowers circulating insulin levels, we hypothesized that the anorexiant effect of insulin could result from insulin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2645
更新日期:1991-05-01 00:00:00
abstract::We previously reported the existence of a descending multisynaptic, pituitary-independent, neural pathway between the hypothalamus and the testes in the male rat. Stimulation of this pathway by the intracerebroventricular (icv) injection of IL-1beta or corticotropin-releasing factor blunts the testosterone (T) respons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1441
更新日期:2004-04-01 00:00:00
abstract::Pulsatile release of GnRH-1 is critical to stimulate gonadotropes of the anterior pituitary. This secretory pattern seems to be inherent to GnRH-1 neurons, however, the mechanisms underlying such episodical release remain unknown. In monkey nasal explants, the GnRH-1 population exhibits synchronized calcium events wit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1711
更新日期:2009-07-01 00:00:00
abstract::The ability of a monoclonal antiidiotypic antibody (clone 1D5) directed against the binding site of a monoclonal antiestradiol antibody to interact with the estrogen receptor (ER) was investigated. The following lines of evidence indicate that clone 1D5 has the capacity of mimicking the actions of estradiol, and recog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597160
更新日期:1992-06-01 00:00:00
abstract::Women with polycystic ovary syndrome (PCOS) and hyperandrogenism have altered hormone levels and suffer from ovarian dysfunction leading to subfertility. We have attempted to generate a model of hyperandrogenism by feeding mice chow supplemented with dehydroepiandrosterone (DHEA), an androgen precursor that is often e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2140
更新日期:2013-12-01 00:00:00
abstract::Although estrogens and estrogen receptors (ERs) are known to function in the male brain and reproductive tract, few studies have evaluated their involvement in the male hematopoietic and immune systems. This study was undertaken to determine the role of ERalpha in hematopoietic progenitor and B lymphocyte maturation. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.7.7560
更新日期:2000-07-01 00:00:00
abstract::Previously, a human CYP3A4-transgenic (Tg-CYP3A4) mouse line was reported to exhibit enhanced metabolism of midazolam by cytochrome P450 3A4 (CYP3A4) expressed in small intestine. Here we show that expression of CYP3A4 and murine cyp3a and cyp2b was both age and sex dependent. CYP3A4 was expressed in the livers of mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1248
更新日期:2005-07-01 00:00:00
abstract::In vivo, fibroblast growth factor-2 (FGF-2) inhibits longitudinal bone growth. Similarly, activating FGF receptor 3 mutations impair growth in achondroplasia and thanatophoric dysplasia. To investigate the underlying mechanisms, we chose a fetal rat metatarsal organ culture system that would maintain growth plate hist...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6032
更新日期:1998-06-01 00:00:00
abstract::Islet β-cell dysfunction that leads to impaired insulin secretion is a principal source of pathology of diabetes. In type 2 diabetes, this breakdown in β-cell health is associated with compromised islet-enriched transcription factor (TF) activity that disrupts gene expression programs essential for cell function and i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa213
更新日期:2021-02-01 00:00:00
abstract::Cultures of Sertoli cells were treated with freshly isolated, intact germ cells to determine if germ cells were capable of influencing Sertoli cell function. By using two-dimensional polyacrylamide gel electrophoresis and autoradiography, germ cells were found to increase several-fold the incorporation of [32P]orthoph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1317
更新日期:1987-04-01 00:00:00
abstract::Impaired trophoblast invasion is associated with pregnancy disorders such as early pregnancy loss and preeclampsia. There is evidence to suggest that the consumption of caffeine during pregnancy may increase the risk of pregnancy loss; however, little is known about the direct effect of caffeine on normal trophoblast ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1930
更新日期:2012-09-01 00:00:00
abstract::To address the neural mediation of the eating-inhibitory effect of circulating glucagon-like peptide-1 (GLP-1), we investigated the effects of 1) intra-fourth ventricular infusion of the GLP-1 receptor antagonist exendin-9 or 2) area postrema lesion on the eating-inhibitory effect of intrameal hepatic portal vein (HPV...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1447
更新日期:2014-05-01 00:00:00
abstract::The effects of pentobarbital (Pnt) treatment on the progesterone (P)-induced afternoon increase in the medial basal hypothalamic (MBH) LHRH and serum LH and FSH levels in ovariectomized estradiol benzoate-primed rats were studied. Pnt injection before P blocked the afternoon rise in serum gonadotropins but failed to a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-4-1299
更新日期:1981-04-01 00:00:00
abstract::We recently reported that long-term treatment with pioglitazone restored cardiac Akt phosphorylation in response to hyperthermia (HT) and subsequent cardiac heat-shock protein 72 (HSP72) expression, in heredity insulin resistance rats via improvement of insulin sensitivity. Because adrenomedullin (AM) promotes Akt pho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1052
更新日期:2009-03-01 00:00:00
abstract::The brain has traditionally been considered to be a target site of peripheral steroid hormones. By contrast, new findings over the past decade have shown that the brain itself also has the capability of forming steroids de novo, the so-called neurosteroids. De novo neurosteroidogenesis in the brain from cholesterol is...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2007-1592
更新日期:2008-06-01 00:00:00
abstract::The expression of the lutropin receptor (LHR) in granulosa cells is a complex phenomenon under the hormonal control of FSH and estradiol. Using primary cultures of granulosa cells from immature female rats pretreated with diethylstilbestrol (a compound with estrogen-like activity), the role of FSH in LHR induction was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6722
更新日期:1999-05-01 00:00:00
abstract::Reduced and S-carbamidomethylated human GH (RCAM-hGH) was digested with human plasmin, yielding a mixture of products. These were partially separated by chromatography on DEAE-cellulose, yielding three major fractions: Da and Db, which were equipotent with native hGH in the weight gain test; and Dc, which was about ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-5-1366
更新日期:1978-05-01 00:00:00
abstract::Hypothalamic-pituitary-thyroid (HPT) axis activity is important for energy homeostasis, and is modified by stress. Maternal separation (MS) alters the stress response and predisposes to metabolic disturbances in the adult. We therefore studied the effect of MS on adult HPT axis activity. Wistar male and female pups we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1239
更新日期:2016-08-01 00:00:00
abstract::The beta-subunit of hCG (hCG beta) displays immunological cross-reactivity with human LH (hLH). A detailed study was undertaken to investigate the effect of chemical and enzymatic modifications on the immunological behavior of hCG beta, particularly on its cross-reactivity with hLH, with a view to obtaining a highly h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-5-1556
更新日期:1980-11-01 00:00:00
abstract::Antisera were raised against synthetic replicates of the carboxyl-terminal (C-terminal) fragment of the precursor to human GH-releasing factor (GRF) (pre-proGRF) whose structure was predicted from the complementary DNA cloned from a pancreatic tumor. These antisera were used along with antisera to human GRF itself to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-156
更新日期:1986-01-01 00:00:00
abstract::Insulin-like growth factors (IGFs), when isolated from serum or tissue fluids, are usually found as part of a protein complex which also contains one of several IGF binding proteins (IGFBPs). Although some IGFBPs have been shown to alter interactions of IGFs with their receptors in vitro and can modify the responses o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7507840
更新日期:1994-02-01 00:00:00