Abstract:
:We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as assessed by quantitative in situ hybridization histochemistry with a specific oligodeoxynucleotide probe. The addition of T3 (40 micrograms/kg) to the PTU-containing diet completely prevented the reduction in NOS transcripts. Hyperthyroidism, induced by adding 160 micrograms/kg T3 to the food, more than doubled the prevalence of NOS transcripts in the PVN and SON after a similar time. Up-regulation of NOS gene transcripts induced by the osmotic stimulus of chronic salt loading was markedly attenuated by PTU-induced hypothyroidism. These results demonstrate a major effect of thyroid status on regulation of NOS gene expression in the hypothalamus.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Ueta Y,Levy A,Chowdrey HS,Lightman SLdoi
10.1210/endo.136.10.7545100subject
Has Abstractpub_date
1995-10-01 00:00:00pages
4182-7issue
10eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Immunoreactive neurophysin (Np) is demonstrated by the immunoperoxidase technique not only within neurones of the classical hypothalamo-neurohypophyseal system, but also in extrahypothalamic perikarya and fibers. Np-positive perikarya are found in the triangular nucleus of the septum. Np fibers join the stria terminal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-1-334
更新日期:1978-01-01 00:00:00
abstract::Many aspects of reproductive function are corrected in hypogonadal mice with preoptic area grafts (HPG/POA). Gonadotropin release and gonadal development are dependent on the presence of GnRH cells within the grafts and GnRH innervation of the median eminence. This study examined the effect of a known modulator of GnR...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2432
更新日期:1991-05-01 00:00:00
abstract::To define further the mechanism by which metoclopramide, a dopamine antagonist, stimulates aldosterone secretion, seven lambs were injected iv with metoclopramide during a constant infusion of 5% dextrose in water and again during a constant infusion of trimethapan, a ganglionic blocker. In addition, suspensions of ad...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-3-887
更新日期:1983-09-01 00:00:00
abstract::The rat S14 gene is synergistically regulated by thyroid hormone and carbohydrates. The 5'-flanking region of this gene contains both carbohydrate and thyroid hormone response elements (TREs). We recently located the carbohydrate response element (CHORE) between -1583 and -1069 bases from the start site of transcripti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8319560
更新日期:1993-07-01 00:00:00
abstract::Testosterone propionate causes a striking increase in in vivo uptake of 2-deoxyglucose by the levator ani muscle of immature male rats. Autoradiography showed the labeled 2-deoxyglucose to be uniformly distributed over the entire muscle. Liquid scintillation counting of whole muscles allowed quantification of 2-deoxyg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-119
更新日期:1983-07-01 00:00:00
abstract::To determine whether glucagon plays a significant role in the restitution of blood volume after hemorrhage, pentobarbital-anesthetized dogs were treated with somatostatin (SRIF). The administration of SRIF (14 micrograms/kg.h) prevented the increase in osmolality and the complete restitution of plasma protein and bloo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-1945
更新日期:1982-06-01 00:00:00
abstract::During in vitro maturation of porcine cumulus cell-oocyte complexes and in vitro luteinization of porcine granulosa cells, FSH induces the expression of the protease TNFα-converting enzyme/A disintegrin and metalloproteinase domain 17 (TACE/ADAM17) and the epidermal growth factor (EGF)-like factors, which activate the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1655
更新日期:2014-03-01 00:00:00
abstract::Myocardial fibrosis is a key pathological change in a variety of heart diseases contributing to the development of heart failure, arrhythmias, and sudden death. Recent studies have shown that relaxin prevents and reverses cardiac fibrosis. Endogenous expression of relaxin was elevated in the setting of heart disease; ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1114
更新日期:2012-08-01 00:00:00
abstract::The adrenal zona glomerulosa (ZG) secretes aldosterone to regulate sodium balance. Chronic sodium restriction increases aldosterone accompanied by ZG expansion. The ZG is innervated by sympathetic, vasoactive intestinal polypeptide (VIP) and neuropeptide tyrosine (NPY), and sensory, calcitonin gene-related peptide, ne...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0487
更新日期:2010-10-01 00:00:00
abstract::In this study, site-directed mutagenesis and biochemical strategies have been used to establish whether disulfide bonding between extracellular Cys residues contributes to the structural integrity of the GnRH receptor (GnRH-R) and, if so, to delineate the nature of the bonding patterns involved. The majority of G prot...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5233
更新日期:1997-07-01 00:00:00
abstract::The action of excitatory amino acid agonists on endogenous somatostatin release was examined in primary cultures of rat diencephalic neurons. Increasing concentrations of glutamate stimulated somatostatin release in a dose-dependent manner. Since this effect was decreased by Mg2+, all experiments were performed in Mg2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2360
更新日期:1988-11-01 00:00:00
abstract::Both Snell (Pit-1(dw) or (dwj), dw/dw) and Ames (Prophet of Pit-1(df), df/df) dwarf mice fail to produce prolactin (PRL) as well as GH due to deficient transcription factor Pit-1 activity and have reduced numbers of hypothalamic PRL-inhibiting area A12 tuberoinfundibular dopaminergic (TIDA) neurons. It has been report...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0931
更新日期:2004-12-01 00:00:00
abstract::We have examined the regulation of adipogenesis of a 3T3-F442A cell variant. The variant, designated 3T3-GH-independent clone 16 (GI-16), was isolated after serum-induced adipogenic commitment. 3T3-GI-16 fibroblasts displayed a lower serum requirement for adipogenesis than the 3T3-F442A parent cell. Insulin-stimulated...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-325
更新日期:1989-01-01 00:00:00
abstract::The production of hydrogen peroxide (H2O2) as an essential process for iodide organification is a key reaction in TSH-induced thyroid hormone synthesis. Here we characterize the signal transduction pathway involved in TSH-induced H2O2 production in FRTL-5 thyroid cells. At higher than 1 nM TSH, N6-(L-2-phenylisopropyl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828520
更新日期:1995-01-01 00:00:00
abstract::Experiments were conducted to obtain information on the possible transfer of TSH and thyroid hormones from mother rats to their infants via milk. The mothers were thyroidectomized by surgical or chemical (tapazole) means to eliminate maternal thyroid hormones from milk while increasing its content of TSH. Their pups w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-800
更新日期:1983-03-01 00:00:00
abstract::There are now robust data supporting the Developmental Origins of Health and Disease (DOHaD) paradigm. This includes human and animal data focusing on nutrition or environmental chemicals during development. However, the term DOHaD has not been generally accepted as the official term to be used when one is concerned w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/EN.2015-1394
更新日期:2015-10-01 00:00:00
abstract::The scaffolding protein, protein targeting to glycogen (PTG), orchestrates the signaling of several metabolic enzymes involved in glycogen synthesis. However, little is known concerning the regulation of PTG itself. In this study, we have cloned and characterized the mouse promoter of PTG. We identified multiple FoxA2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1513
更新日期:2006-07-01 00:00:00
abstract::L-Asparaginase (ASNase), a drug widely used in the treatment of acute lymphoblastic leukemia, has been reported to decrease serum T4-binding globulin (TBG) levels, while results of serum albumin determinations were conflicting. This effect in vivo has been attributed to depressed liver protein synthesis, but this hypo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-3-1185
更新日期:1986-09-01 00:00:00
abstract::Seven adult female rhesus monkeys were laparotomized at days 22, 42, and 157 of pregnancy and blood was collected from the uterine vein and peripheral circulation. Plasma samples were analyzed for monkey chorionic gonadotropin (mCG), monkey chorionic somatomammotropin (mCS), and prolactin by radioimmunoassay. Levels o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-851
更新日期:1977-03-01 00:00:00
abstract::We investigated the effect of epidermal growth factor (EGF) on collagen and protein synthesis in clone MC3T30-E1, a cell line which retains osteoblast-like characteristics. EGF at concentrations of 2-50 ng/ml significantly the hydroxyproline content of the cell layer. These effects were completely abolished by the add...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1810
更新日期:1982-12-01 00:00:00
abstract::Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2421
更新日期:1987-06-01 00:00:00
abstract::In the present report, we show that prostaglandin (PG) E2 is the most potent factor which stimulates aromatase expression via cyclic AMP and promoter II. PGE2 acts via EP1 and EP2 receptor subtypes to stimulate both the PKC and PKA pathways. The combined stimulation of both of these pathways results in maximal express...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940410
更新日期:1996-12-01 00:00:00
abstract::Estradiol imprinting plays an important role in the regulation of galanin (GAL) gene expression in the rat. In the anterior pituitary gland, GAL gene expression is greatly induced by estrogen. The relative involvement that the two estrogen receptor subtypes, alpha and beta, have in regulating this induction is not kno...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.6.6932
更新日期:1999-06-01 00:00:00
abstract::In rodents, kisspeptin-expressing neurons are localized in 2 hypothalamic brain nuclei (anteroventral periventricular nucleus/periventricular nucleus continuum [AVPv/PeN] and arcuate nucleus [ARC]) and modulated by sex steroids. By using wild-type (WT) and aromatase knockout (ArKO) mice (which cannot convert testoster...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1120
更新日期:2013-08-01 00:00:00
abstract::GnRH neurons form the final common pathway regulating the secretion of gonadotropins from the anterior pituitary. Since the patterns of gonadotropin release display profound sexual dimorphism among mammals including the rodent, we undertook an ultrastructural analysis to determine whether these neurosecretory cells we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-695
更新日期:1990-02-01 00:00:00
abstract::Epidermal growth factor (EGF) and TGFalpha are well known for their activities in the ovary. Both factors initiate signal transduction by binding to the common EGF receptor that has been demonstrated in the ovary across vertebrates from fish to humans. Using zebrafish as the model, we demonstrated in the present study...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8579
更新日期:2002-01-01 00:00:00
abstract::Differences in the catalytic activity of the placental and gonadal isozymes of porcine aromatase cytochrome P450 (P450arom) were examined in cell lines exhibiting stable expression of recombinant enzyme. Cell lines were selected that expressed high, but similar, immunodetectable levels of each isozyme based on Western...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7140
更新日期:1999-11-01 00:00:00
abstract::We have recently reported evidence for the presence of stereospecific energy-dependent transport processes for T3 in rat tissues. These processes were responsible for maintenance of concentration differences of free L- and D-T3 across the cellular plasma and nuclear membranes. In rat liver, the free L-T3 concentration...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-6-2449
更新日期:1985-12-01 00:00:00
abstract::The marked up-regulation of oxytocin (OT) receptors in rabbit amnion at term was reproduced in cultured amnion cells by raising intracellular cAMP levels. (Bu)2AMP, forskolin, or cholera toxin caused 2- to 8-fold increases in specific binding of iodinated OT antagonist. The rise in OT receptors involves activation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8380370
更新日期:1993-01-01 00:00:00
abstract::Consumption of a low-protein, high-carbohydrate diet induces a striking increase in circulating fibroblast growth factor-21 (FGF21), which is associated with improved cardiometabolic health and increased longevity. Increased lifespan during this dietary protein "dilution" has been explained by resource-mediated trade-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00331
更新日期:2017-10-01 00:00:00