当前位置： SCI文献检索 > ENDOCRINOLOGY期刊下所有文献 > The insulin receptors of chick embryo show tissue-specific structural differences which parallel those of the insulin-like growth factor I receptors.

The insulin receptors of chick embryo show tissue-specific structural differences which parallel those of the insulin-like growth factor I receptors.

Abstract：

:Since specific binding to receptors and biological effects of insulin and insulin-like growth factors (IGFs) are demonstrable soon after the neural tube closes and organogenesis begins in the chick embryo, in the present study we have analyzed the structural characteristics and specificity of the receptors for insulin and IGFs at this early stage of development. We show that membranes from newly differentiated chick embryo tissues (day 6 brain, day 6 heart, day 8 liver, day 12 skeletal muscle) as well as whole embryos postneurulation (day 2, stage of 27-30 somites) have two populations of receptors with distinct specificity: insulin and type I IGF (IGF-I) receptors. Both insulin and IGF-I alpha-subunits, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, had tissue-dependent heterogeneity in Mr (liver greater than heart = skeletal muscle greater than brain) ranging from 138 kilodaltons (kDa) to 129 kDa. Desialylation of the receptors by treatment with neuraminidase produced a significant change in the Mr of the alpha-subunits in liver and heart but not in brain or the whole day 2 embryo. In each tissue the pattern for insulin receptors and IGF-I receptors was strikingly similar. Our studies raise the possibility that postranslational modifications of the insulin and IGF-I receptors, characteristic of terminally differentiated tissues, are already present in early organogenesis. Further, structural heterogeneity of the binding subunit of these receptors among tissues appears to be widespread and not exclusive to the brain receptor. An insulin receptor with features similar to the neural type is the only one detected in embryos at the beginning of organogenesis (day 2). The functional implication of this developmental tissue-specific regulation of insulin and IGF-I receptors, is still speculative. Its possible importance is suggested by the fact that it occurs embryologically early and affects both insulin and IGF-I receptors in parallel.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Bassas L,de Pablo F,Lesniak MA,Roth J

doi

10.1210/endo-121-4-1468

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

1468-76

issue

4

eissn

0013-7227

issn

1945-7170

journal_volume

121

pub_type

杂志文章

相关文献

ENDOCRINOLOGY文献大全