Abstract:
:Rat sexual maturation is preceded by a reduction of the interpulse interval (IPI) of GnRH neurosecretion. This work aims at studying disruption of that neuroendocrine event in females after early exposure to a very low dose of bisphenol A (BPA), a ubiquitous endocrine disrupting chemical. Female rats were exposed to vehicle or BPA 25 ng/kg·d, 25 μg/kg·d, or 5 mg/kg·d from postnatal day (PND)1 to PND5 or PND15. Exposure to 25 ng/kg·d of BPA for 5 or 15 days was followed by a delay in developmental reduction of GnRH IPI studied ex vivo on PND20. After 15 days of exposure to that low dose of BPA, vaginal opening tended to be delayed. In contrast, exposure to BPA 5 mg/kg·d for 15 days resulted in a premature reduction in GnRH IPI and a trend toward early vaginal opening. RNA sequencing analysis on PND20 indicated that exposure to BPA resulted in opposing dose effects on the mRNA expression of hypothalamic genes involved in gamma aminobutyric acid A (GABAA) neurotransmission. The study of GnRH secretion in vitro in the presence of GABAA receptor agonist/antagonist confirmed an increased or a reduced GABAergic tone after in vivo exposure to the very low or the high dose of BPA, respectively. Overall, we show for the first time that neonatal exposure to BPA leads to opposing dose-dependent effects on the neuroendocrine control of puberty in the female rat. A very low and environmentally relevant dose of BPA delays neuroendocrine maturation related to puberty through increased inhibitory GABAergic neurotransmission.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Franssen D,Gérard A,Hennuy B,Donneau AF,Bourguignon JP,Parent ASdoi
10.1210/en.2015-1937subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1740-50issue
5eissn
0013-7227issn
1945-7170journal_volume
157pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Intact 23 kilodalton (kDa), rat PRL is enzymatically cleaved in many target tissues to a 16 kDa (16K PRL) and an 8 kDa fragment. After reduction of an internal disulfide bond the fragments are released. 16K PRL was shown to be a potent mitogen on mammary epithelial cells via PRL receptors. Since estradiol-induced prol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-896
更新日期:1991-08-01 00:00:00
abstract::Glucocorticoids (GCs) are potent regulators of energy metabolism. Chronic GC exposure suppresses brown adipose tissue (BAT) thermogenic capacity in mice, with evidence for a similar effect in humans. Intracellular GC levels are regulated by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) activity, which can amplify...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1722
更新日期:2017-06-01 00:00:00
abstract::The present work extends our previous report that the intracerebroventricular (i.c.v.) injection of interleukin-1beta(IL-1beta, 80 ng) significantly blunted the testosterone response to 1 U/kg human CG (hCG), an effect that we attributed to the stimulation of inhibitory pathways connecting the hypothalamus to the test...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.7.6090
更新日期:1998-07-01 00:00:00
abstract::We have previously established the value of 2-dimensional electrophoretic mRNA activity profiles for investigating the hepatic genomic response to several metabolic perturbations, such as thyroid hormone or GH treatment, diabetes, high carbohydrate diet, starvation, and uremia. We now report the effects of adrenalecto...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2669
更新日期:1985-06-01 00:00:00
abstract::The Caenorhabditis elegans death susceptibility gene, ced-3, has a number of homologs in vertebrate species, including interleukin-1 beta (IL-1 beta)-converting enzyme (ICE), Ich-1long, and CPP32. These genes, which encode a family of related proteases, have been shown to induce apoptosis when transfected into eukaryo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588240
更新日期:1995-11-01 00:00:00
abstract::The insulin receptor, following insulin stimulation of cells, triggers formation of various signaling complexes. In rat HTC hepatoma cells overexpressing normal human insulin receptors (HTC-IR), p85 regulatory subunit of phosphatidylinositol-3-kinase (PI3K) forms signaling complexes containing the insulin receptor, in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6019
更新日期:1998-05-01 00:00:00
abstract::Centrally administered histamine (HA) stimulates the secretion of adenohypophysial POMC-derived peptides, which subsequently cause release of corticosterone. The effect of HA on POMC-derived peptide release is indirect, and it is possible that hypothalamic neurons containing corticotropin-releasing hormone (CRH), argi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070360
更新日期:1994-09-01 00:00:00
abstract::ACTH and cAMP stimulate steroidogenesis and the mitochondrial electron transport system for steroid hydroxylation in cultured mouse adrenal cortex tumor cells. During this stimulation, the biosynthesis of adrenodoxin, a non-heme iron protein which is one of the electron transport enzymes, was examined. 14C-labeled adr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-4-977
更新日期:1976-10-01 00:00:00
abstract::Periconceptional undernutrition (PCUN) results in an earlier prepartum activation of the pituitary-adrenal axis in twin compared with singleton fetuses. We have tested the hypotheses that the functional development of the fetal sheep adrenal is delayed in twins compared with singletons in early gestation and that PCUN...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0761
更新日期:2007-04-01 00:00:00
abstract::Localized expression of cytochromes P450 17 alpha-hydroxylase (P450c17) and aromatase (P450arom) was investigated in embryonic cell layers of elongating porcine blastocysts by immunocytochemistry. Blastocysts were flushed from the uterus on day 12 of pregnancy, fixed in paraformaldehyde, embedded in paraffin, sectione...
journal_title:Endocrinology
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1210/endo.135.6.7988410
更新日期:1994-12-01 00:00:00
abstract::The feedback sites of estrogen within the hypothalamus and lower brain stem involved in the suppression of pulsatile LH secretion during 48-h fasting were examined in ovariectomized rats with local estradiol (E2) implants. The animals were ovariectomized and immediately implanted stereotaxically with stainless steel c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070380
更新日期:1994-09-01 00:00:00
abstract::Local release of TGF-beta during times of high bone turnover leads to elevated levels within the bone microenvironment, and we have shown that TGF-beta suppresses osteoclast apoptosis. Therefore, understanding the influences of TGF-beta on bone resorbing osteoclasts is critical to the design of therapies to reduce exc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0813
更新日期:2010-04-01 00:00:00
abstract::Human myometrium contains hCG/LH receptors. There are fewer of these receptors during labor compared to no labor at preterm or term deliveries. Exogenous hCG can directly inhibit oxytocin-stimulated human myometrial contractions. These findings suggest that hCG may directly maintain myometrial quiescence during pregna...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988470
更新日期:1994-12-01 00:00:00
abstract::The two phases of the ovulatory menstrual cycle of the primate are characterized by divergent activities of the GnRH pulse generator. During the luteal phase, LH pulse frequency is significantly reduced below that observed during the follicular phase. In this report we investigate whether the decrease in pulse frequen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-2044
更新日期:1987-05-01 00:00:00
abstract::Immunoreactive beta-endorphin (ir-beta EP) and immunoreactive N-acetyl-endorphin (ir-NacEP) have been demonstrated in the rat thyroid by specific RIA and characterized by reverse phase HPLC. In addition, pituitary and thyroid ir-beta EP and ir-NacEP levels have been determined after manipulation of the pituitary-thyro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-642
更新日期:1986-08-01 00:00:00
abstract::Adrenals express a high level of neutral cholesteryl ester hydrolase (CEH) activity, and male rats have greater activity than females; however, the identity of the enzyme(s) responsible for this activity and the basis for the sex differences are unknown. Using mice in which hormone-sensitive lipase (HSL) was inactivat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8693
更新日期:2002-03-01 00:00:00
abstract::IGF-I mediates growth-promoting actions of GH. In the present study we investigated whether the somatostatin analog octreotide blunts the stimulatory effects of GH and/or IGF-I on bone growth in hypophysectomized rats infused for 6 d with vehicle, GH, or IGF-I. We found that octreotide significantly suppressed the GH-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.8.8970
更新日期:2002-08-01 00:00:00
abstract::To ascertain if reductions in fetal plasma cortisol cause increases in fetal plasma ACTH, we treated pregnant ewes or their fetuses with aminoglutethimide (10 mg/kg BW) and metyrapone (20 mg/kg BW) and measured the hormonal responses with RIAs. When given to fetuses (n = 9) at 0.90 +/- 0.01 gestation (term-145 days), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1307
更新日期:1988-09-01 00:00:00
abstract::We have studied calmodulin (CaM)-like activity and calcium (Ca++)-regulated phosphodiesterase (PDE) activity in cells from the rat adrenal zona glomerulosa (ZG) and zona fasciculata (ZF). Boiled cell sonicates from the ZG and ZF activated CaM-deficient PDE in a dose-dependent fashion by 2.0- to 2.3-fold. The propertie...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-2-485
更新日期:1983-08-01 00:00:00
abstract::Exposure to environmental toxicants during fetal development alters gene expression and promotes disease later in life. Di-(2-ethylhexyl) phthalate (DEHP) is a plasticizer widely used for the manufacturing of consumer products. Exposure to DEHP has been associated with obesity, asthma, and low T levels. In utero expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1436
更新日期:2015-01-01 00:00:00
abstract::Neuropeptide Y (NPY) readily stimulates the release of hypothalamic LHRH and pituitary LH release in intact and gonadal steroid-primed gonadectomized rats. We have now tested the hypothesis that the release and synthesis of hypothalamic NPY may be regulated by gonadal steroids. To measure the effects of gonadal hormon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1375900
更新日期:1992-06-01 00:00:00
abstract::Metastasis of various malignant cells is inversely related to the abundance of the Nm23-H1 protein. The role of estrogens in tumor metastasis has now been investigated by examining the effect of E2 on the expression of the Nm23-H1 gene. Three human breast carcinoma cell lines, in which endogenous ERalpha is expressed ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.2.8620
更新日期:2002-02-01 00:00:00
abstract::The relationship between serum levels of rat GH (rGH), rat PRL (rPRL), corticosterone, estrogen, and testosterone and the liver-binding sites specific to [125I]iodo-human GH (hGH), -human PRL (hPRL), and -rPRL were investigated in normal rats, in rats bearing the GH- and PRL-secreting tumor (GH3), and in rats 14 days ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2053
更新日期:1978-12-01 00:00:00
abstract::Regularly menstruating female rhesus monkeys were implanted sc with Silastic tubing packed with estrone. The implants were replaced every 6-8 months to maintain the serum estrone and estradiol concentrations constantly elevated 1.5-2.5-fold, i.e. in the midfollicular phase range, for 4 yr. Increasing the estrone and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2373
更新日期:1989-05-01 00:00:00
abstract::We performed an electrophysiological study to investigate ion transport of pendrin and thereby understand the pathogenesis of Pendred syndrome. Using pendrin-transfected COS-7 cells, we could show that pendrin transports both iodide and chloride measured as voltage-dependent inward and outward membrane currents. Chlor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0048
更新日期:2004-09-01 00:00:00
abstract::The effects of nerve activation and of the catecholamines epinephrine and norepinephrine on adrenal corticosteroid release were investigated in intact isolated perfused pig adrenals with preserved nerve supply. To study the contact zones of medullary and cortical tissues, porcine adrenals were examined on the histolog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-900
更新日期:1990-08-01 00:00:00
abstract::The effects of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF alpha) on basal and TRH-induced TSH release, and the effects of IL-1 beta on the uptake of [125I]T3 and [125I]T4 and on nuclear binding of [125I]T3 were examined. Furthermore, the release of other anterior pituitary hormones in the pres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612490
更新日期:1996-05-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::Many human breast tumors are driven by high intratumor concentrations of 17beta-estradiol that appear to be locally synthesized. The role of aromatase is well established, but the possible contribution of the steroid sulfatase (STS), which liberates estrogens from their biologically inactive sulfates, has been inadequ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.4.8091
更新日期:2001-04-01 00:00:00