Abstract:
:The signals that regulate cardiac myocyte maturation in the neonatal heart are not completely understood. In our study we examined the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] on primary cultures of ventricular myocytes isolated from neonatal rat hearts. Our data show that 1,25-(OH)2D3 inhibited an increase in both the muscle-specific form of creatine kinase and V1 myosin isoenzyme levels in myocytes induced to mature by serum withdrawal. Thus, in contrast to other cell types studied to date, in the heart, 1,25-(OH)2D3 blocks cell maturation. Treating cultures with phorbol 12-myristate 13-acetate induced a decrease in the muscle-specific isoenzyme of creatine kinase similar to the effects of 1,25-(OH)2D3. Interestingly, we found that staurosporine, a protein kinase-C inhibitor, blunts the effects of 1,25-(OH)2D3 and has the opposite effect of phorbol 12-myristate 13-acetate on cultured myocytes induced to mature by serum withdrawal. Thus, our data identify a novel role for 1,25-(OH)2D3 in the regulation of myocardial development and suggest that 1,25-(OH)2D3 may be acting through a protein kinase-dependent mechanism to maintain cardiac myocytes in an immature state.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
O'Connell TD,Giacherio DA,Jarvis AK,Simpson RUdoi
10.1210/endo.136.2.7835280subject
Has Abstractpub_date
1995-02-01 00:00:00pages
482-8issue
2eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We have examined the regulation of adipogenesis of a 3T3-F442A cell variant. The variant, designated 3T3-GH-independent clone 16 (GI-16), was isolated after serum-induced adipogenic commitment. 3T3-GI-16 fibroblasts displayed a lower serum requirement for adipogenesis than the 3T3-F442A parent cell. Insulin-stimulated...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-325
更新日期:1989-01-01 00:00:00
abstract::Kisspeptin regulates reproductive events, including puberty and ovulation, primarily via GnRH neurons. Prolonged treatment of prepubertal striped bass females with kisspeptin (Kiss) 1 or Kiss2 peptides failed to enhance puberty but suggested a gnrh-independent pituitary control pathway. Kiss2 inhibited, but Kiss1 stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1894
更新日期:2014-05-01 00:00:00
abstract::Linear growth results from proliferation and differentiation of chondrocytes within the growth plates and is regulated, in part, by the insulin-like growth factors (IGFs). IGF binding proteins (IGFBPs) also appear to play a significant, but yet unclear, role. To examine IGFBP production by chondrocytes, we isolated bo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.7688290
更新日期:1993-08-01 00:00:00
abstract::Gastrin is natriuretic, but its renal molecular targets and signal transduction pathways are not fully known. In this study, we confirmed the existence of CCKBR (a gastrin receptor) in male human renal proximal tubule cells and discovered that gastrin induced S6 phosphorylation, a downstream component of the phosphati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1813
更新日期:2013-02-01 00:00:00
abstract::Previous studies have established the presence of an extrahypothalamic GnRH in a variety of tissues. GnRH receptor is known to be present in the placenta, which produces and secretes the decapeptide from the very early stages of placentation. We hypothesized that GnRH may play a role in the preimplantation development...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6899
更新日期:1999-08-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) and interleukin-1 beta (IL-1) are secreted by activated monocytes and other immune cells. Since IL-1 has been shown to elevate rat plasma ACTH and both of these cytokines induce similar acute-phase responses, the present studies of TNF were undertaken to characterize the ACTH response...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3131
更新日期:1989-06-01 00:00:00
abstract::The prevailing view of sexual differentiation of mammalian brain is that androgen synthesized in the fetal and neonatal testis and aromatized centrally during a perinatal sensitive period is the sole source of brain estradiol and the primary determinant of sex differences. Subregions of the diencephalon are among the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0607
更新日期:2011-01-01 00:00:00
abstract::The circadian rhythm of blood corticosterone was examined in rats whose feeding was restricted to a specific time of day. The peak of the corticosterone level was found just before feeding time regardless of whether, the rats were kept on a 12-h light, 12-h dark cycle or in constant light or were blinded. It took at l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-3-918
更新日期:1980-03-01 00:00:00
abstract::We have established a system, the CA77 rat medullary thyroid carcinoma cell line, for studying the products of somatostatin (SS) gene expression. Based on the amino acid sequence of proSS, we developed a RIA for the amino terminus of proSS (proSS-NTP) and demonstrated in acidic cell extracts two major proSS-NTP-contai...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-218
更新日期:1986-01-01 00:00:00
abstract::Age-associated changes in the reproductive hormones-the gonadal steroid hormones and the gonadotropins-have been identified as potential risk factors for Alzheimer's disease (AD). However, levels of gonadotropins and estrogens are closely linked in vivo, and it has proven difficult to separate the effects of gonadotro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1168
更新日期:2010-11-01 00:00:00
abstract::Cholera toxin (CT) injected ip on day 1 (day of ovulation) of the 4-day hamster estrous cycle, when circulatory progesterone is high and estrogen low, induced a massive uterine decidual reaction, a progesterone-dependent growth normally triggered by the implanting blastocyst. However, CT injected ip on day 3, when cir...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-2095
更新日期:1983-06-01 00:00:00
abstract::The metabolism of cholesterol sulfate (5-cholesten-3 beta-ol sodium sulfate) and cholesterol to pregnenolone sulfate and pregnenolone, respectively, in human fetal adrenal mitochondria has been investigated. Cholesterol sulfate (50 microM) was converted efficiently to pregnenolone sulfate (5-pregnen-3 beta-ol-20-one s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-1-208
更新日期:1982-07-01 00:00:00
abstract::Diabetic cardiomyopathy is characterized by myocyte loss and myocardial fibrosis, leading to decreased elasticity and impaired contractile function. The study examines the downstream signaling whereby oxidative stress, induced by hyperglycemia, leads to myocardial fibrosis and impaired contractile function in the left...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0877
更新日期:2008-01-01 00:00:00
abstract::We have confirmed the serum calcium-raising effect of adrenalectomy in young male rats 5-6 h after parathyroidectomy that was first observed by others many years ago. (The phenomenon has also been reported in cats, dogs, and mice.) In addition, we have shown that adrenalectomy raises the serum ionized calcium as well ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2259
更新日期:1991-05-01 00:00:00
abstract::In virtually all studies with MA-10 cells, progesterone RIAs have been used to measure steroid synthesis. To test whether progesterone is a stable end product, we investigated the metabolism of added tritiated progesterone and pregnenolone in MA-10 cells over a period of 3 h. Steroids were then extracted, separated by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8545
更新日期:2001-12-01 00:00:00
abstract::Notch signaling directs cell fate during embryogenesis by influencing cell proliferation, differentiation, and apoptosis. Notch genes are expressed in the adult mouse ovary, and roles for Notch in regulating folliculogenesis are beginning to emerge from mouse genetic models. We investigated how Notch signaling might i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0213
更新日期:2009-02-01 00:00:00
abstract::The absence of TSH-stimulated radioiodide uptake in differentiated thyroid cancer is associated with a high recurrence rate and reduced survival. We studied regulation of the sodium/iodide symporter gene in human papillary thyroid cancer cell lines (BHP) and primary human thyroid cells. BHP cells expressed very low le...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8344
更新日期:2001-08-01 00:00:00
abstract::Protein kinase-C (PKC) has been suggested as a possible mediator of the antigonadotropic action of prostaglandin F2 alpha (PGF2 alpha) in luteal cells. To examine this possibility, we evaluated the effects of phorbol ester [12-O-tetradecanoylphorbol-13-acetate (TPA)] in relation to those of PGF2 alpha on cAMP accumula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1388
更新日期:1990-03-01 00:00:00
abstract::There are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium L-glutamate (MSG). In our study, newborn male pups were ip injected with MSG (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0640
更新日期:2006-03-01 00:00:00
abstract::The purpose of these experiments was to test the hypothesis that estrogen has an anabolic effect on cancellous bone formation in long bones of growing rats. In the present studies, estrogen decreased measurements related to cancellous bone formation, including osteoblast number, fluorochrome-labeled bone perimeter, an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243320
更新日期:1993-12-01 00:00:00
abstract::The importance of locally produced insulin-like growth factor-I (IGF-I) in connective tissues has recently been recognized. It has been postulated that the action of anabolic hormones on bone may be mediated through local IGF-I release. However, whether IGF-I can also be modulated by other locally acting cytokines has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-4-1635
更新日期:1990-10-01 00:00:00
abstract::TSH is the most important biomarker in the interpretation of thyroid function in man. Its levels are determined by circulating thyroid hormone (TH) levels that feed back centrally to regulate the expression of the subunits that comprise TSH from the pituitary. The nuclear corepressor 1 (NCoR1), is a critical coregulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1504
更新日期:2012-10-01 00:00:00
abstract::17 beta-Estradiol (E2; 100 micrograms/kg X day) was infused iv for 6 days into three chronically catheterized ovine fetuses beginning at 105-106 days of gestation (term, 147 days) and into four younger fetuses for 3 days commencing at 87-92 days. Control studies were performed on three fetuses at each age. At 90 days,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-5-1618
更新日期:1983-05-01 00:00:00
abstract::The effect of diabetes mellitus on the synthesis and secretion of thyroid hormone ws investigated in mice with streptozotocin-induced diabetes. Thyroid glands were labeled in vivo with 131I for 2 h. In control animals, TSH stimulated the synthesis of PB127I and 131I-labeled iodothyronines and simultaneously decreased ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-5-1428
更新日期:1981-11-01 00:00:00
abstract::The present investigation was undertaken to examine the possible role of cAMP in PTH-stimulated prostaglandin (PG) production in organ cultures of neonatal mouse parietal bones. Cultures were treated with PTH, forskolin, isobutylmethylxanthine (IBMX), and 8-bromo-cAMP (8BrcAMP). We found that similar concentrations of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-1070
更新日期:1990-02-01 00:00:00
abstract::Follistatin (FS), which binds to the inhibin/activin beta A- or beta B-subunit is localized with and modulates the biological actions of activin in many systems. However, in contrast to the wide distribution of the activin beta-subunit proteins and messenger RNAs (mRNA) in the brain, demonstration of FS mRNA signal ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612560
更新日期:1996-05-01 00:00:00
abstract::The TSH receptor (TSHR) is constitutively active and is further enhanced by TSH ligand binding or by stimulating TSHR antibodies (TSHR-Abs) as seen in Graves' disease. TSH is known to activate the thyroid epithelial cell via both Galphas-cAMP/protein kinase A/ERK and Galphaq-Akt/protein kinase C coupled signaling netw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0878
更新日期:2009-01-01 00:00:00
abstract::Adipokines, cytokines mainly produced by adipocytes, are active participants in the regulation of inflammation. Administration of zymosan (ZY) was used to investigate the regulation and role of adipokines during peritonitis in mice. Injection of ZY led to a significant increase in leptin levels in both serum and perit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0327
更新日期:2008-08-01 00:00:00
abstract::Diets lacking soy and alpha-alpha derivatives that are considered to be estrogen-free by standard bioassays (uterotrophic assay and vaginal opening) have been revealed to contain considerable amounts of compounds able to transcriptionally activate the estrogen receptors (ERs) and stimulate luciferase expression in sev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0660
更新日期:2005-12-01 00:00:00
abstract::The metabolism of 25-hydroxycholesterol (25-OH-cholesterol) to progestins by mitochondria and dispersed cells prepared from ovaries of PMSG-hCG-primed rats was studied. Mitochondria converted [3H]25-OH-cholesterol into [3H]pregnenolone and [3H]progesterone. Unlabeled 25-OH-cholesterol also stimulated mitochondrial ste...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1785
更新日期:1982-12-01 00:00:00