Abstract:
:Previous studies have established the presence of an extrahypothalamic GnRH in a variety of tissues. GnRH receptor is known to be present in the placenta, which produces and secretes the decapeptide from the very early stages of placentation. We hypothesized that GnRH may play a role in the preimplantation development of embryos. To examine this hypothesis, we assessed GnRH and GnRH receptor messenger RNA (mRNA; RT-PCR) and protein expression (Immunohistochemistry) in preimplantation murine embryos at various developmental stages. Furthermore, preimplantation murine embryos were cultured with GnRH agonist and antagonist in vitro to assess the influence of GnRH analogs on embryo development. GnRH is expressed in the developing mouse embryo from morula to hatching blastocyst stages at the mRNA and protein levels. GnRH receptor mRNA is also present in the developing embryos studied. Preimplantation embryonic development was significantly enhanced by incubation with increasing concentrations of GnRH agonist and is significantly decreased by GnRH antagonist compared with that in the control group. Moreover, GnRH antagonist (5 and 10 microM) was able to completely block embryo development. The deleterious effect of GnRH antagonist on embryo development was reversed by increasing concentrations of the agonist, as determined by the number of embryos reaching the blastocyst stage.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Raga F,Casañ EM,Kruessel J,Wen Y,Bonilla-Musoles F,Polan MLdoi
10.1210/endo.140.8.6899subject
Has Abstractpub_date
1999-08-01 00:00:00pages
3705-12issue
8eissn
0013-7227issn
1945-7170journal_volume
140pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::An in vitro system which uses perifused pieces from the antrum of rat stomach has been used to study the effect of dihydrosomatostatin on gastrin release. The gastrin concentration in fractions of the perfusate was measured by radioimmunoassay. Dihydrosomatostatin (2 x 10-7M) had no effect on basal gastrin levels but ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1374
更新日期:1975-06-01 00:00:00
abstract::Glucose release from the liver is mediated by hypothalamic norepinephrine (NE) neuronal activity, but glucose itself (or a metabolite of it) exerts negative feedback effects on central NE activity. The aim of the present study was to investigate a possible role for insulin in these central glucose homeostatic mechanis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1590
更新日期:1985-10-01 00:00:00
abstract::We previously found that the sustained rise in active sodium transport by the toad urinary bladder after exposure to insulin is associated with the synthesis of a membrane protein with a molecular weight of 25,000. Studying the effects of insulin on the incorporation of uridine into RNA of epithelial cells of the blad...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-2167
更新日期:1981-12-01 00:00:00
abstract::We report here 1) that GnRH and a potent GnRH agonist inhibit basal and LH stimulated ovarian androgen biosynthesis in vitro, 2) that the inhibitory effects of GnRH are dose-dependent and completely inhibited by concomitant treatment with a GnRH antagonist and 3) that the GnRH action is very rapid (t1/2 = 10 min) and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-661
更新日期:1981-08-01 00:00:00
abstract::The nuclear receptor steroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential regulator of endocrine development and function, and the expression of the corresponding gene (sf-1/ad4bp) is precisely regulated in a time- and tissue-dependent manner. We previously demonstrated that the basal promoter...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1305
更新日期:2011-05-01 00:00:00
abstract::We have investigated the production and actions of a growth regulatory protein, insulin-like growth factor binding protein (IGFBP)-3, in the androgen-responsive prostate carcinoma cell line LNCaP. Confluent monolayers of cells secreted approximately 0.7 ng/ml IGFBP-3 over 24 h. Dihydrotestosterone (DHT, 10 nM) and 1,2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.7.7534
更新日期:2000-07-01 00:00:00
abstract::p-Tyramine, an endogenous amine with sympathomimetic action, is found in the mammalian hypothalamus. When injected ip, p-tyramine reduced serum PRL without altering LH and TSH serum titers in adult orchidectomized rats and rats subjected to ether or immobilization stress. The hyperprolactinemia achieved by this last p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-5-2044
更新日期:1985-05-01 00:00:00
abstract::The pregnane X receptors (PXRs) and the constitutive androstane receptor (CAR) were initially isolated as nuclear receptors regulating xenobiotic metabolism and elimination, alleviating chemical insults. However, recent works suggest that these xenoreceptors play an endobiotic role in modulating hepatic lipid metaboli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1435
更新日期:2010-04-01 00:00:00
abstract::A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts as has the noncanonical behavior of IGF-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1464
更新日期:2015-12-01 00:00:00
abstract::Thyroid hormone entry into the thymocyte, a thyroid hormone target, was investigated by incubating the cells with tracer amounts of [125I]L-T3. At 37 C T3 uptake was linear with time up to 2 min, and then approached a plateau. The specific T3 uptake, obtained by subtracting the uptake in the presence of excess unlabel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2443
更新日期:1989-05-01 00:00:00
abstract::Bioactive peptides are usually synthesized as inactive precursor proteins that yield bioactive products only after specific biosynthetic processing events. Large dense core vesicles (LDCV) are usually the site of storage of mature peptides. Atrial myocyte LDCV are rather unique in their storage of intact prohormone, p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5629
更新日期:1997-12-01 00:00:00
abstract::Control of trabecular bone volume is mediated by local events. Recently, it has been shown that the cytokines interleukin-1 (IL-1), tumor necrosis factor alpha (TNF alpha), and TNF beta [also called lymphotoxin (LT)] produced by immune cells all stimulate osteoclast activity, whereas the lymphokine interferon-gamma (I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2494
更新日期:1987-06-01 00:00:00
abstract::The binding properties of the gamma-emitting 125I-labeled 11 beta-methoxy analog of 16 alpha-iodoestradiol, 11 beta-methoxy-16 alpha-iodoestradiol (MIE2), were characterized for its use in vivo as a ligand for the measurement and localization of estrogen-binding sites. In binding displacement studies, MIE2 bound to ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2074
更新日期:1989-05-01 00:00:00
abstract::The relationship between serum levels of rat GH (rGH), rat PRL (rPRL), corticosterone, estrogen, and testosterone and the liver-binding sites specific to [125I]iodo-human GH (hGH), -human PRL (hPRL), and -rPRL were investigated in normal rats, in rats bearing the GH- and PRL-secreting tumor (GH3), and in rats 14 days ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-2053
更新日期:1978-12-01 00:00:00
abstract::PTH-related protein (PTHrP) has been shown to be a major factor responsible for hypercalcemia of malignancy. PTHrP acts via the PTH/PTHrP receptor, and therefore, PTH antagonists might be expected to reverse the hypercalcemia in malignancy. In the present studies, the PTH antagonists [Tyr34]bovine (b) PTH-(7-34)NH2, [...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156920
更新日期:1994-05-01 00:00:00
abstract::Orexins/hypocretins heavily innervate the posterior division of the paraventricular nucleus of the thalamus (pPVT), which expresses both orexin receptor types. The pPVT is important for adaptations to repeated stress, particularly the ability to facilitate to novel stress after repeated stress exposure. Here, we exami...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1652
更新日期:2011-12-01 00:00:00
abstract::A competitive protein-binding (CPB) assay, suitable for measuring corticosterone levels in 20 mul of mouse plasma or 100 mg of brain, is described. The postnatal development of adrenocortical function was determined in C57BL/10 and DBA/1 mice by CPB assay of basal and stressinduced levels of plasma corticosterone and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-6-1434
更新日期:1976-06-01 00:00:00
abstract::1Alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) is a potent mediator of differentiation and maintenance of specific functions of osteoblasts. To detect novel targets for 1,25-(OH)2D3 action, we applied differential display PCR to human fetal osteoblast-like cells and identified the human analog of murine cystein rich pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5954
更新日期:1998-04-01 00:00:00
abstract::To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP-B r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1309330
更新日期:1992-01-01 00:00:00
abstract::The cell of origin of human null cell pituitary adenomas is disputed. Although these tumors, by definition, do not produce any of the anterior pituitary hormones in vivo, they have been shown to express gonadotropin subunit genes, release gonadotropin hormones in vitro, and express the gonadotroph-associated transcrip...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.7.6100
更新日期:1998-07-01 00:00:00
abstract::ACTH produced a 75% increase in pregnenolone biosynthesis from endogenous precursors in isolated cells prepared from the rat Snell adrenocortical carcinoma 494. On the addition of 24- and 25-hydroxycholesterol to the tumor cells, the rate of pregnenolone synthesis increased 10-fold but was insensitive to the presence ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-5-1230
更新日期:1979-11-01 00:00:00
abstract::The effects of rat CRF, arginine vasopressin (VP), oxytocin (OXY), and isoproterenol (ISO) on the biosynthesis and release of pro-ACTH/endorphin-derived peptides by monolayer cultures of rat anterior pituitary cells in complete serum-free medium (CSFM) were studied. When cells were exposed to hormone for 3 h, CRF, VP,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-953
更新日期:1987-03-01 00:00:00
abstract::The purpose of this study was to examine how the physical characteristics of light influence the short photoperiod-induced atrophy of the reproductive system. In the first experiment, groups of Syrian hamsters were exposed to a 14-h light, 10-h dark (14:10) photoperiod, a 11:13 photoperiod or a 11:13 photoperiod exten...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-648
更新日期:1986-08-01 00:00:00
abstract::We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as asses...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7545100
更新日期:1995-10-01 00:00:00
abstract::Congenital hypothyroidism with thyroid dysgenesis (TD) is a frequent human condition characterized by elevated levels of TSH in response to reduced thyroid hormone levels. Congenital hypothyroidism is a genetically heterogeneous disease. In the majority of cases studied, no causative mutations have been identified and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0882
更新日期:2005-12-01 00:00:00
abstract::Canine renal cortical PTH receptors were solubilized after occupancy of membrane-associated receptors with the agonist ligand [125I]bovine (b) PTH-(1-34). Stabilization of binding during solubilization required the use of high concentrations of BSA (optimally 5%) and appropriate detergents (0.5% 3-[(3-cholamidopropyl)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-932
更新日期:1986-03-01 00:00:00
abstract::Glucagon-like peptide 1 (7-36)amide (GLP-1) is an insulinotropic intestinal peptide hormone with a potential role as antidiabetogenic therapeutic agent. It mediates a potentiation of glucose-induced insulin secretion, by activation of adenylate cyclase and subsequent elevation of cytosolic free calcium, [Ca2+]cyt. We ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6947
更新日期:1999-09-01 00:00:00
abstract::The in vitro production of total corticosteroids, corticosterone (B), and cortisol (F) by cultured adrenocortical cells from fetuses (115-120 days old) and newborn lambs exposed to ACTH-(1-24) was investigated in relation to the time of culture. Corticosteroids were measured by using an antibody against F, whereas B a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1128
更新日期:1984-04-01 00:00:00
abstract::Biological actions of natriuretic peptide (NP) are determined by the condition of the receptor as well as that of the hormone. Although we previously demonstrated in hypertensive rats the up-regulation of NP-A receptor that mediates various biological actions of NPs, the pathophysiologic significance of NP-C receptor,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.3.8603580
更新日期:1996-03-01 00:00:00
abstract::Most studies of lipoprotein requirements for steroid secretion by the adrenal have examined the mixed cell population of the whole gland; none have examined lipoprotein requirements of guinea pig adrenocortical cells. In this study the effect of exogenous lipoprotein on the ability of cells from each of the different ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-640
更新日期:1987-02-01 00:00:00