Abstract:
:To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP-B receptor or guanylate cyclase-B (GC-B), and the clearance receptor (C-receptor), we characterized the receptor preparations from human, bovine, and rat tissues and cultured cells with the aid of the binding assay, Northern blot technique, and the cGMP production method. Using these receptor preparations, we examined the binding affinities of ANP, BNP, and CNP for the C-receptor and their potencies for cGMP production via the ANP-A receptor (GC-A) and the ANP-B receptor (GC-B). These analyses revealed the presence of a marked species difference in the receptor selectivity of the natriuretic peptide family, especially among BNPs. Therefore, we investigated the receptor selectivity of the natriuretic peptide family using the homologous assay system with endogenous ligands and receptors of the same species. The rank order of binding affinity for the C-receptor was ANP greater than CNP greater than BNP in both humans and rats. The rank order of potency for cGMP production via the ANP-A receptor (GC-A) was ANP greater than or equal to BNP much greater than CNP, but that via the ANP-B receptor (GC-B) was CNP greater than ANP greater than or equal to BNP. These findings on the receptor selectivity of the natriuretic peptide family provide a new insight into the understanding of the physiological and clinical implications of the natriuretic peptide system.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Suga S,Nakao K,Hosoda K,Mukoyama M,Ogawa Y,Shirakami G,Arai H,Saito Y,Kambayashi Y,Inouye Kdoi
10.1210/endo.130.1.1309330subject
Has Abstract,Author List Incompletepub_date
1992-01-01 00:00:00pages
229-39issue
1eissn
0013-7227issn
1945-7170journal_volume
130pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::PTH-related protein (PTHrP) is expressed in a stretch-responsive manner in several types of smooth muscle. We previously demonstrated the production of PTHrP in adult rat heart muscle. In this study, we demonstrate the production of PTHrP in the cardiovascular systems of several mammalian species, including human. We ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013360
更新日期:1994-07-01 00:00:00
abstract::Obesity is associated with systemic inflammation and elevated levels of TNFα, leading to impaired glucose tolerance. In humans, obesity is also associated with reduced nutrient-stimulated secretion of the intestinal incretin hormone, glucagon-like peptide-1 (GLP-1). We hypothesized that TNFα plays a direct role in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1361
更新日期:2015-11-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) and interleukin-1 beta (IL-1) are secreted by activated monocytes and other immune cells. Since IL-1 has been shown to elevate rat plasma ACTH and both of these cytokines induce similar acute-phase responses, the present studies of TNF were undertaken to characterize the ACTH response...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3131
更新日期:1989-06-01 00:00:00
abstract::Dysregulation of the hypothalamic-pituitary-adrenocortical axis has been theoretically linked to the processes of aging for decades. To investigate the effects of age on high frequency rhythms of plasma ACTH at the time of circadian activation, integrated 2-min blood samples were collected over 4 h in 10 young and 14 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8393770
更新日期:1993-08-01 00:00:00
abstract::The ability of different LH-like hormones, such as hCG, PMSG/equine (e) CG, ovine (o) LH, eLH, and rat (r) LH, to bind to and stimulate steroidogenesis in two types of rat gonadal cells was studied under the same experimental conditions. In both Leydig and granulosa cells, the maximal steroidogenic responses elicited ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-597
更新日期:1985-02-01 00:00:00
abstract::Activin A is a dimeric protein hormone that regulates numerous cellular functions. A clear physiological role for this molecule in pregnancy is suggested by previous studies in the human, wherein activin A rises dramatically as women approach parturition. To determine whether the rodent is a suitable animal model for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5231
更新日期:1997-07-01 00:00:00
abstract::PTH-related protein (PTHrP) has been shown to be a major factor responsible for hypercalcemia of malignancy. PTHrP acts via the PTH/PTHrP receptor, and therefore, PTH antagonists might be expected to reverse the hypercalcemia in malignancy. In the present studies, the PTH antagonists [Tyr34]bovine (b) PTH-(7-34)NH2, [...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156920
更新日期:1994-05-01 00:00:00
abstract::In order to study whether changes in adenohypophysial dopamine are only related to PRL release or also to the variation of LH or FSH, dopamine (DA) in the pars distalis was measured during the estrous cycle and after castration of both male and female rats. Catecholamines were assayed using a highly sensitive radioenz...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1682
更新日期:1980-06-01 00:00:00
abstract::We investigated whether genetic selection for growth influences pituitary GH secretion in two strains of rats, LL (large) and SS (small). Animals were bled every 15 min for 6 h via an indwelling atrial Silastic catheter, and GH levels were determined by RIA. LL and SS males displayed a low frequency, high amplitude ep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-1948
更新日期:1986-11-01 00:00:00
abstract::Male rats of five different strains (Simonsen albino, Wistar, Long-Evans, Holtzman Sprague-Dawley, and Charles River Sprague-Dawley) were tested for their response to the U.S. Biochemical Corp. Remington low iodine diet containing 15-18 microgram I/kg. Measurements made after the diet had been fed for 28-30 days indic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-458
更新日期:1981-08-01 00:00:00
abstract::The effects of L-leucine, D-leucine, and L-isoleucine upon the secretion of glucagon and insulin were investigated using the isolated, perfused rat pancreas. All experiments were conducted in the presence of 5.6 mM D-glucose. Ten-minute perfusions of 2, 5, and 10 mM L-leucine induced the release of glucagon and insuli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1208
更新日期:1978-10-01 00:00:00
abstract::T4-binding globulin (TBG), the principal carrier of thyroid hormone in serum, is a glycoprotein containing 20% carbohydrate. The importance of the carbohydrate moiety has been previously studied by enzymatic deglycosylation, which showed that deglycosylated TBG retains its original immunological an T4-binding properti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1614
更新日期:1986-04-01 00:00:00
abstract::In male rats maintained on a 12 h light-dark schedule (6 AM-6 PM), there is a nyctohemeral cycle of plasma prolactin which consists of a nadir at 11:30 AM and an apogee at approximately 11:30 PM. In rats exposed to constant darkness, this rhythm persists for 7 days. Seven days of constant light, however, reverses this...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-5-1230
更新日期:1975-05-01 00:00:00
abstract::Enhanced epinephrine secretion from adrenal chromaffin cells (ACCs) is an important homeostatic response to severe systemic inflammation during sepsis. Evidence suggests that increased activation of ACCs by preganglionic sympathetic neurons and direct alterations in ACC function contribute to this response. However, t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1623
更新日期:2014-01-01 00:00:00
abstract::A novel class of targeted chemotherapeutic agents based on cytotoxic compounds linked to LHRH analogs was tested in a long term superfusion system in order to determine their effects on different types of rat pituitary cells. The compounds investigated included two cytotoxic LHRH agonists, T-98 ([D-Lys6]LHRH coupled t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.5.8477650
更新日期:1993-05-01 00:00:00
abstract::Photoaffinity-labeled androgen-binding protein (ABP), present in cytosol prepared from the caput epididymidis of intact sexually mature rabbits, was produced using 17 beta-hydroxy-[1,2-3H]4,6-androstadien-3-one (delta 6-testosterone). Photo-labeled ABP could not be distinguished from unlabeled ABP by gel filtration ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-4-1270
更新日期:1982-10-01 00:00:00
abstract::Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5275
更新日期:1997-07-01 00:00:00
abstract::Insulin receptors were measured in cultured Zajdela rat hepatoma cells (ZHC cells), a stable cell line which presents differentiated hepatic functions. The number of sites was 50,000/cell at 2 C, and the dissociation constant for high affinity binding was 1.6 x 10(-10) M. Down-regulation of receptors occurred rapidly ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-3-993
更新日期:1982-09-01 00:00:00
abstract::The most significant increase in metabolic syndrome over the previous decade occurred in women of reproductive age, which is alarming given that metabolic syndrome is associated with reproductive problems including subfertility and early pregnancy loss. Individuals with metabolic syndrome often consume excess fructose...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1618
更新日期:2016-02-01 00:00:00
abstract::Anterior pituitary cells from adult male rats were cultured on a natural extracellular matrix (ECM). From the 5th day, spindle-shaped cells, fusing to form elongated fibers, were observed among the epithelial cells. These fibers later increased in size and number and were identified as striated muscle fibers, based on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-3002
更新日期:1988-06-01 00:00:00
abstract::Species-specific differences in genes encoding type II GnRH receptor indicate that a functional hepta-helical receptor is produced in monkeys but not in rodents, cows, chimpanzees, or humans. To further investigate the extent of evolutionary differences, we sequenced the type II GnRH receptor gene from wild-type Soay ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1625
更新日期:2004-05-01 00:00:00
abstract::This review describes formation of the islet basement membrane and the function of extracellular matrix (ECM) components in β-cell proliferation and survival. Implications for islet transplantation are discussed. The insulin-producing β-cell is key for maintaining glucose homeostasis. The islet microenvironment greatl...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2019-00206
更新日期:2019-08-01 00:00:00
abstract::Protein iodination in the thyroid is largely confined to the surface of the epithelium. Intracellular iodine binding is insignificant. We have tested our hypothesis that the key mechanism in the control of intracellular iodination is the control of the intracellular availability of H2O2. The sites of iodination were i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5222
更新日期:1997-07-01 00:00:00
abstract::The PRL-stimulating effect of metoclopramide (2-methoxy-5-chloroprocainamide) was examined in normal and adrenalectomized male rats pretreated with DL-p-chlorophenylalanine methyl ester (PCPA), an inhibitor of serotonin biosynthesis. PCPA significantly reduced the hypothalamic content of serotonin, increased basal PRL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1622
更新日期:1981-05-01 00:00:00
abstract::Prolonged exposure to elevated glucocorticoid levels is known to produce insulin resistance (IR), a hallmark of diabetes mellitus. Although not fully elucidated, the underlying molecular mechanisms by which glucocorticoids induce IR may provide potential targets for pharmacological interventions. Here we characterized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1214
更新日期:2008-02-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) blocks LH-stimulated androstenedione production by immature rat theca-interstitial cells (TIC) in vitro. The mechanism for TNF inhibition of LH-induced androstenedione is unknown and was investigated. LH stimulation of androstenedione synthesis in TIC is mediated via a cAMP-dependent ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404680
更新日期:1993-11-01 00:00:00
abstract::Increased expression of bone morphogenetic proteins (BMPs) in several tissues is associated with inflammation and type 2 diabetes mellitus. BMP2 and BMP4 mRNA expression is increased in pancreatic islets from db/db mice and β-cell proliferation and function are inhibited by BMP4. The effect of BMPs on α-cells is curre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1163
更新日期:2016-10-01 00:00:00
abstract::Relaxin is a naturally occurring regulator of collagen turnover. In this study, we determined the role of endogenous relaxin in the pathogenesis of primary tubulointerstitial fibrosis after unilateral ureteric obstruction (UUO). Four- to 6-wk-old relaxin (RLX) gene-knockout (RLX(-/-)) and age-matched wild-type (RLX(+/...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0814
更新日期:2007-02-01 00:00:00
abstract::Endocrine cells isolated from the anterior pituitary fire intracellular Ca2+ ([Ca2+]i) transients due to voltage-gated Ca2+ entry. However, the patterns of [Ca2+]i transients within the glandular parenchyma of the anterior pituitary are unknown. Here we describe, using real-time confocal laser microscopy, several spon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7363
更新日期:2000-03-01 00:00:00
abstract::Intraluteal infusion of the prostaglandin (PG) synthesis inhibitor, sodium meclofenamate (Mec) causes premature luteolysis in rhesus monkeys. To evaluate further the actions of PG synthesis inhibitors in primate luteal function, we examined the in vitro effects of Mec and another inhibitor, flurbiprofen (Flur), on PG,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1380
更新日期:1990-03-01 00:00:00