Abstract:
:A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts as has the noncanonical behavior of IGF-1R. Here we report studies conducted in lung fibroblasts from female wild-type C57/B6 (TSHR(+/+)) mice and their littermates in which TSHR has been knocked out (TSHR(-/-)). Flow cytometric analysis revealed that cell surface IGF-1R levels are substantially lower in TSHR(-/-) fibroblasts compared with TSHR(+/+) fibroblasts. Confocal immunofluorescence microscopy revealed similar divergence with regard to both cytoplasmic and nuclear IGF-1R. Western blot analysis demonstrated both intact IGF-1R and receptor fragments in both cellular compartments. In contrast, IGF-1R mRNA levels were similar in fibroblasts from mice without and with intact TSHR expression. IGF-1 treatment of TSHR(+/+) fibroblasts resulted in reduced nuclear and cytoplasmic staining for IGF-1Rα, whereas it enhanced the nuclear signal in TSHR(-/-) cells. In contrast, IGF-1 enhanced cytoplasmic IGF-1Rβ in TSHR(-/-) fibroblasts while increasing the nuclear signal in TSHR(+/+) cells. These findings indicate the intimate relationship between TSHR and IGF-1R found earlier in human orbital fibroblasts also exists in mouse lung fibroblasts. Furthermore, the presence of TSHR in these fibroblasts influenced not only the levels of IGF-1R protein but also its subcellular distribution and response to IGF-1. They suggest that the mouse might serve as a suitable model for delineating the molecular mechanisms overarching these two receptors.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Atkins SJ,Lentz SI,Fernando R,Smith TJdoi
10.1210/en.2015-1464subject
Has Abstractpub_date
2015-12-01 00:00:00pages
4731-40issue
12eissn
0013-7227issn
1945-7170journal_volume
156pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The marked up-regulation of oxytocin (OT) receptors in rabbit amnion at term was reproduced in cultured amnion cells by raising intracellular cAMP levels. (Bu)2AMP, forskolin, or cholera toxin caused 2- to 8-fold increases in specific binding of iodinated OT antagonist. The rise in OT receptors involves activation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8380370
更新日期:1993-01-01 00:00:00
abstract::Recent studies have revealed that C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth. Nevertheless, the effect of CNP on bone turnover has not yet been well studied. To elucidate this issue, we investigated the bone phenotype of a mouse model with elevated plasma CNP concentrations (SA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1801
更新日期:2015-07-01 00:00:00
abstract::This study was undertaken to identify uterine insulin-like growth factor-I (IGF-I) receptors and examine the regulation of these receptors throughout the estrous cycle and after 17 beta-estradiol (E2) administration to immature rats. We have demonstrated type I IGF receptors in crude uterine membranes by binding and c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-597
更新日期:1989-08-01 00:00:00
abstract::Brown adipose tissue (BAT) is a major site of nonshivering thermogenesis in mammals. Rodent studies indicated that BAT thermogenic activity may protect against obesity. Recent findings using novel radiodiagnosis procedures revealed unanticipated high activity of BAT in adult humans. Moreover, complex processes of cell...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2013-1403
更新日期:2013-09-01 00:00:00
abstract::Acidosis has been implicated in the pathogenesis of various metabolic bone diseases. Experimental acid ingestion is known to stimulate the release of bone mineral in humans and increase osteoclastic resorption surfaces in animals, but the mechanism has remained unclear. We have investigated the effect of medium pH on ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-1-119
更新日期:1986-07-01 00:00:00
abstract::The evolution of the IGF binding protein (IGFBP) gene family has been difficult to resolve. Both chromosomal and serial duplications have been suggested as mechanisms for the expansion of this gene family. We have identified and annotated IGFBP sequences from a wide selection of vertebrate species as well as Branchios...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0047
更新日期:2011-06-01 00:00:00
abstract::Previous studies from this laboratory have demonstrated the presence of two populations of rat Leydig cells (I and II), which differ in their capacity for hCG- or cAMP-stimulated testosterone production. In the present study, we examined the metabolism of 25-hydroxycholesterol in primary cultures of both populations o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-1184
更新日期:1985-09-01 00:00:00
abstract::GH and IGF-I are potent regulators of muscle growth and function. Although IGF-I is known to mediate many of the effects of GH, it is not yet clear whether all effects of GH are completely dependent on the IGF-I system. To evaluate the biological effects of the GH/IGF-I axis on muscle growth, we administrated recombin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0906
更新日期:2005-04-01 00:00:00
abstract::UMR106-06 cells predominantly express the C1a isoform of the rat calcitonin (CT) receptor (CTR). We have compared the homologous regulation of the C1a CTR endogenously expressed in UMR106-06 cells with the cloned C1a CTR in transfected HEK 293 cells, in which expression is driven by a heterologous promoter. It was fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895320
更新日期:1996-11-01 00:00:00
abstract::In adult male rhesus monkeys, 1 day of fasting leads to a profound suppression of LH secretion that is rapidly reversed with refeeding. To test the hypothesis that changes in insulin secretion during fasting and refeeding mediate the changes in LH secretion, blood samples for LH measurement were obtained between 0900-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641210
更新日期:1996-06-01 00:00:00
abstract::The physicochemical and immunological properties of androgen receptors from kidney and brain of testicular-feminized (Tfm) mutant mice and wild-type mice were compared. Analysis by gel filtration and sucrose density gradients revealed that the mol wt of the mutant receptor was 66K (38A; 3.8S) which was significantly s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-771
更新日期:1989-02-01 00:00:00
abstract::Tuberoinfundibular peptide is a recently discovered agonist for the PTH receptor-2; the latter has a wide distribution including the external zone of the median eminence of the hypothalamus, suggesting a role in neuroendocrine function. We have investigated the effects of tuberoinfundibular peptide on the hypothalamo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8308
更新日期:2001-08-01 00:00:00
abstract::Small amounts of TRH are present in the thyroid, and TRH has been shown in several studies to have a moderate direct inhibitory effect on TSH-stimulated thyroid hormone secretion. In a recent publication, however, data were presented to demonstrate that very low doses of TRH given in short pulses induce immediate incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-385
更新日期:1988-02-01 00:00:00
abstract::Estrogen receptor alpha and beta proteins (ERalpha and ERbeta) at various stages of development of the rat mammary gland were quantified by Western blotting. ERalpha and ERbeta recombinant proteins were used as standards, and their molar concentrations were measured by ligand binding assays. In 3-week-old pregnant, la...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8260
更新日期:2001-07-01 00:00:00
abstract::Regulation of MUC1 expression and removal is a salient feature of embryo implantation, bacterial clearance, and tumor progression. In some species, embryo implantation is accompanied by a transcriptional decline in uterine epithelial expression of MUC1. In other species, MUC1 is locally removed at blastocyst attachmen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0399
更新日期:2004-09-01 00:00:00
abstract::To understand the physiological role of ghrelin, it is crucial to study both the actions of ghrelin and the regulation of ghrelin secretion. Although ghrelin actions have been extensively revealed, the direct factors regulating ghrelin secretion by ghrelin-producing cells (X/A-like cells), however, is not fully unders...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1455
更新日期:2011-07-01 00:00:00
abstract::The health impacts of endocrine-disrupting chemicals (EDCs) remain debated, and their tissue and molecular targets are poorly understood. In this study, we leveraged systems biology approaches to assess the target tissues, molecular pathways, and gene regulatory networks associated with prenatal exposure to the model ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00817
更新日期:2019-02-01 00:00:00
abstract::A variety of human malignancies overexpresses isoform A of the insulin receptor (IR-A) and produces IGFs (IGF-I and/or IGF-II). IR-A binds IGF-II with high affinity (although 4-fold lower than that for insulin), whereas it binds IGF-I with low affinity (approximately 30-fold lower than that for insulin). However, in e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0377
更新日期:2009-08-01 00:00:00
abstract::1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] and 24,25-(OH)2D3 differentially affect the specific activity of alkaline phosphatase (ALPase) and phospholipase-A2 (PLA2) of plasma membranes and extracellular matrix vesicles produced by costochondral reserve zone and growth zone cartilage chondrocytes in culture. In the prese...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2878
更新日期:1988-12-01 00:00:00
abstract::Endocannabinoids act as a major neuromodulatory system in a variety of physiological and behavioral functions. Three major lines of evidence suggest that the endocannabinoid system interacts with gonadal hormones. First, the endocannabinoid system is implicated in behaviors and physiological functions that are known t...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2011-1643
更新日期:2012-03-01 00:00:00
abstract::IGF-II has been reported to decrease neonatal islet cell apoptosis and in vitro adult islet cell necrosis and apoptosis, but the usefulness of IGF-II in a transplantation setting is unknown. We evaluated the effect of in vitro IGF-II incubations on microencapsulated rat islet survival both in vitro and in minimal mass...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0185
更新日期:2003-07-01 00:00:00
abstract::This is an obituary for Gregory Goodwin Pincus whose death in August, 1967, terminated a distinguished career in the biological sciences. His major work was in reproductive biology. This work, in collaboration with Dr. John Rock, led to the development and field testing in Puerto Rico of the 1st OC (oral contracepti...
journal_title:Endocrinology
pub_type: 传,历史文章,杂志文章
doi:10.1210/endo-82-4-651
更新日期:1968-04-01 00:00:00
abstract::Folliculostellate (FS) cells are known to communicate with each other and with endocrine cells via gap junctions in the anterior pituitary. We investigated whether TGFbeta3 and estradiol, known to regulate FS cell production and secretion of basic fibroblast growth factor (bFGF), increases gap junctional communication...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0122
更新日期:2005-09-01 00:00:00
abstract::The Rice H500 rat Leydig cell tumor is a well characterized model of humoral hypercalcemia of malignancy (HHM). Circulating concentrations of PTH-related protein (PTHRP) have not been reported in this or any other animal model of HHM. Taking advantage of the marked N-terminal amino acid homology between rodent and hum...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-3-1444
更新日期:1990-09-01 00:00:00
abstract::MCF-7 cells migrate through vitronectin-coated filters in response to insulin-like growth factor I (IGF-I); migration is inhibited by the matrix metalloproteinase (MMP) inhibitor BB-94, but not by the serine proteinase inhibitor aprotinin. MMP-9 was identified in the conditioned medium of MCF-7 cells; in addition, flu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6623
更新日期:1999-04-01 00:00:00
abstract::Plasma concentrations of IGF-1 decrease markedly during starvation secondary to a reduction in somatotropic receptors in the liver. We investigated whether IGF-1 administration during starvation in mice inhibits the catabolic state normally observed. Plasma concentrations of IGF-1 in starved mice receiving IGF-1 thera...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2793
更新日期:1989-11-01 00:00:00
abstract::The present study was designed to evaluate the relative contribution of endogenous excitatory amino acids to the control of the estradiol-induced LH surge using specific blockers for N-methyl-D-aspartic acid (NMDA) and non-NMDA receptor types. Adult female rats ovariectomized for 2-3 weeks were implanted with third ve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1771
更新日期:1990-03-01 00:00:00
abstract::The secretory pattern of GH secretion is markedly sexually dimorphic in the adult rat. The patterning of GH secretion is determined by the coordinated activity of somatostatin (SS)- and GH-releasing hormone (GHRH)-containing neurosecretory cells located in the hypothalamus. In this study we examined whether there is s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2369
更新日期:1991-05-01 00:00:00
abstract::The rat prostate is dependent on androgen for normal growth and differentiation. In addition, the organ undergoes rapid cell death upon withdrawal of androgen on castration, and the atrophied tissue is capable of regrowth after androgen replacement in adult animals. In our search for novel factor(s) that participate i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.9.7685
更新日期:2000-09-01 00:00:00
abstract::Members of the winged helix transcription factor family are known to regulate epithelial cell differentiation by regulating cell-specific gene expression. rWIN is a newly discovered member of the winged helix family shown to be present in the adult rat testis. In the testis the human homolog of rWIN, HFH-11, was local...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.8.7599
更新日期:2000-08-01 00:00:00