Abstract:
:Kisspeptin regulates reproductive events, including puberty and ovulation, primarily via GnRH neurons. Prolonged treatment of prepubertal striped bass females with kisspeptin (Kiss) 1 or Kiss2 peptides failed to enhance puberty but suggested a gnrh-independent pituitary control pathway. Kiss2 inhibited, but Kiss1 stimulated, FShβ expression and gonadal development, although hypophysiotropic gnrh1 and gnrh receptor expression remained unchanged. In situ hybridization and immunohistochemistry on brains and pituitaries revealed a differential plasticity between the 2 kisspeptin neurons. The differences were most pronounced at the prespawning phase in 2 regions along the path of gnrh1 axons: the nucleus lateralis tuberis (NLT) and the neurohypophysis. Kiss1 neurons appeared in the NLT and innervated the neurohypophysis of prespawning males and females, reaching Lh gonadotropes in the proximal pars distalis. Males, at all reproductive stages, had Kiss2 innervations in the NLT and the neurohypophysis, forming large axonal bundles in the former and intermingling with gnrh1 axons. Unlike in males, only preovulatory females had massive NLT-neurohypophysis staining of kiss2. Kiss2 neurons showed a distinct appearance in the NLT pars ventralis-equivalent region only in spawning zebrafish, indicating that this phenomenon is widespread. These results underscore the NLT as important nuclei for kisspeptin action in 2 facets: 1) kisspeptin-gnrh interaction, both kisspeptins are involved in the regulation of gnrh release, in a stage- and sex-dependent manner, especially at the prespawning phase; and 2) gnrh-independent effect of Kiss peptides on the pituitary, which together with the plastic nature of their neuronal projections to the pituitary implies that a direct gonadotropic regulation is plausible.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Zmora N,Stubblefield J,Golan M,Servili A,Levavi-Sivan B,Zohar Ydoi
10.1210/en.2013-1894subject
Has Abstractpub_date
2014-05-01 00:00:00pages
1874-86issue
5eissn
0013-7227issn
1945-7170journal_volume
155pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Sertoli cells regulate differentiation and development of the testis and are essential for maintaining adult testis function. To model the effects of dysregulating Sertoli cell number during development or aging, we have used acute diphtheria toxin-mediated cell ablation to reduce Sertoli cell population size. Results...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00196
更新日期:2017-09-01 00:00:00
abstract::Neuropeptide Y (NPY) is highly expressed in the hypothalamus, where it regulates feeding and energy homeostasis. Interestingly, NPY and its receptors are also expressed in peripheral tissues with roles in metabolism, including pancreatic islets. In islets, NPY is known to suppress insulin secretion acutely. In additio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1537
更新日期:2014-12-01 00:00:00
abstract::Axonal degeneration often leads to the death of neuronal cell bodies. Previous studies demonstrated the substantial protective role of 17β-estradiol (E2) in several types of neuron. However, most studies examined cell body protection, and the role of 17β-E2 in axonal degeneration of retinal ganglion cells (RGC) remain...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0046
更新日期:2011-07-01 00:00:00
abstract::We have compared the influence of insulin-like growth factor 1 (IGF-1) on pituitary gene expression in the rat cell lines GH4C1 and GH3. Incubation with IGF-1 increased PRL messenger RNA (mRNA) levels in GH4C1 cells by 4- to 5-fold but decreased the levels of PRL transcripts in GH3 cells. In addition, the levels of GH...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5585
更新日期:1997-12-01 00:00:00
abstract::The proliferation and differentiation of testicular progenitor stem cells into highly specialized germ cells (spermatozoa) are largely controlled by the hormonally (FSH and testosterone) regulated adjacent supporting Sertoli cells. However, the factors involved in this control remain largely unknown. In the present st...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.8.7625
更新日期:2000-08-01 00:00:00
abstract::Endocrine cells isolated from the anterior pituitary fire intracellular Ca2+ ([Ca2+]i) transients due to voltage-gated Ca2+ entry. However, the patterns of [Ca2+]i transients within the glandular parenchyma of the anterior pituitary are unknown. Here we describe, using real-time confocal laser microscopy, several spon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7363
更新日期:2000-03-01 00:00:00
abstract::Pdx-1 plays a key role in the development of the pancreas and the control of islet gene transcription and has also been proposed as a dominant regulator of the alpha- vs. beta-cell phenotype via extinction of proglucagon expression. To ascertain the relationship between Pdx-1 and proglucagon gene expression, we examin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0495
更新日期:2005-01-01 00:00:00
abstract::The LHX3 transcription factor is required for pituitary and nervous system development in mammals. Mutations in the human gene are associated with hormone-deficiency diseases. The gene generates two mRNAs, hLHX3a and hLHX3b, which encode three proteins with different properties. Here, the cis elements and trans-acting...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0970
更新日期:2006-01-01 00:00:00
abstract::The maternal plasma concentrations of GH and PRL increase dramatically upon the initiation of lactation in the rat. In light of the fact that these two hormones have evolved from one common precursor, we sought to determine if the neuroendocrine mechanisms regulating their concomitant increase during lactation are com...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-464
更新日期:1989-01-01 00:00:00
abstract::We examined the effects of bombesin on rat pancreatic digestive enzyme gene expression using cloned complementary DNA probes for amylase, trypsinogen I, chymotrypsinogen B, and lysophospholipase. Rats were injected sc three times daily with 5 nmol/kg body wt bombesin. Pancreata were investigated after 6, 12, 24, 48, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1371450
更新日期:1992-03-01 00:00:00
abstract::Leukemia inhibitory factor (LIF) is a pleiotropic cytokine known to control the proliferation and survival of stem cells including primordial germ cells and gonocytes. This led us to study the origin and regulation of testicular LIF. The LIF transcript was detected in the rat testis by RT-PCR from 13.5 days postcoitum...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.3.7399
更新日期:2000-03-01 00:00:00
abstract::Insulin resistance and type 2 diabetes mellitus are associated with impaired postprandial secretion of glucagon-like peptide-1 (GLP-1), a potent insulinotropic hormone. The direct effects of insulin and insulin resistance on the L cell are unknown. We therefore hypothesized that the L cell is responsive to insulin and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0726
更新日期:2009-02-01 00:00:00
abstract::The metabolic consequences of visceral obesity have been associated with amplification of glucocorticoid action by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in adipose tissue. This study aimed to assess in a rat model of diet-induced obesity the effects of pharmacological 11beta-HSD1 inhibition on the m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1199
更新日期:2007-05-01 00:00:00
abstract::A biologically active form of the epidermal growth factor (EGF) precursor has been detected in human fluids and secretions. The secreted protein identified in human urine and milk has an apparent molecular mass of 160-170 kilodaltons and exhibits an affinity for the glycosaminoglycan heparin. More importantly, the sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419140
更新日期:1993-01-01 00:00:00
abstract::We report for the first time the expression of estrogen receptor-related receptor (ERR)-alpha in fetal and adult rat chondrocytes in growth plate and articular cartilage and the rat chondrogenic cell line C5.18 cells in vitro. ERRalpha mRNA and protein were expressed from proliferating chondrocyte to mature chondrocyt...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0962
更新日期:2007-03-01 00:00:00
abstract::An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77,000, its N-terminal amino acid sequence is the same as prothrombin's for 15 residues, it contains gamma-carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-rea...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-167
更新日期:1990-01-01 00:00:00
abstract::Despite understanding the molecular basis of activin/TGF beta and bone morphogenetic protein (BMP) signaling, this study is the first to characterize multiple, sequential elements of these pathways in the ovary concurrently. The expression of activin/BMP receptor, Smad, and beta glycan mRNAs by postnatal rat ovaries w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.4.8728
更新日期:2002-04-01 00:00:00
abstract::Selenoproteins contain the essential trace element selenium whose deficiency leads to major disorders including cancer, male reproductive system failure, or autoimmune thyroid disease. Up to now, 25 selenoprotein-encoding genes were identified in mammals, but the spatiotemporal distribution, regulation, and function o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1246
更新日期:2011-11-01 00:00:00
abstract::Little is known about the mechanisms of action of polypeptide hormones on chromatin structure and nuclear function. We have employed micrococcal nuclease to examine the effect of TSH on the accessibility of DNA in thyroid nuclei. Brief digestion of nuclear suspensions with 0.05-0.2 U/ml micrococcal nuclease at 26-28 C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-2118
更新日期:1982-06-01 00:00:00
abstract::Glucocorticoids and nonsteroidal antiinflammatory drugs (NSAIDs) are widely used for the treatment of inflammatory and immune diseases. Nitric oxide (NO) has a diversity of physiological functions, but its excess production has been implicated in the inflammatory process. The present study was designed to elucidate th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6718
更新日期:1999-05-01 00:00:00
abstract::Gastrin is natriuretic, but its renal molecular targets and signal transduction pathways are not fully known. In this study, we confirmed the existence of CCKBR (a gastrin receptor) in male human renal proximal tubule cells and discovered that gastrin induced S6 phosphorylation, a downstream component of the phosphati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1813
更新日期:2013-02-01 00:00:00
abstract::Prolactin (PRL) shares several characteristics with growth factors and cytokines, many of which are known to bind to heparan sulfate proteoglycans. In this study we examined the heparin-binding properties of selected members of the PRL/GH family, using heparin affinity columns followed by gel electrophoresis/Western b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6677
更新日期:1999-02-01 00:00:00
abstract::The structural requirements for the inhibition of net bone collagen synthesis by parathyroid hormone (PTH) in vitro have been examined by study of the effects of selected fragments and analogs of bovine PTH (bPTH) upon the incorporation of [3H]proline into collagenase-digestible and -nondigestible proteins by neonatal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-103
更新日期:1981-01-01 00:00:00
abstract::Basal or hypothalamic extract-induced ACTH or TSH secretory capacity as well as intracellular ACTH content were assessed in cultured rat adenohypophyseal cells after various pretreatments of the donor animals. Medium and intracellular ACTH or TSH were measured by radioimmunoassay. Ten-day to 2-month adrenalectomy enha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-3-717
更新日期:1976-03-01 00:00:00
abstract::The effect of diabetes mellitus on the synthesis and secretion of thyroid hormone ws investigated in mice with streptozotocin-induced diabetes. Thyroid glands were labeled in vivo with 131I for 2 h. In control animals, TSH stimulated the synthesis of PB127I and 131I-labeled iodothyronines and simultaneously decreased ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-5-1428
更新日期:1981-11-01 00:00:00
abstract::GnRH is detectable in the cerebrospinal fluid (CSF), but its source remains unidentified. Previous studies have harvested CSF for GnRH analysis from the median eminence region, but it is unknown whether GnRH in CSF is restricted to this region. If CSF-GnRH plays a physiological role, through volume transmission, to co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1636
更新日期:2008-10-01 00:00:00
abstract::Immunohistochemical (immunoperoxidase) studies were performed on 478 sections from 97 rat pituitary glands with rabbit antisera to unconjugated human synthetic calcitonin beta-endorphin, and/or ACTH-(17--39). Calcitonin-positive cells were present in a majority of the anterior lobes studied, whereas they were present ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1966
更新日期:1980-06-01 00:00:00
abstract::Recent studies from our laboratory (Endocrinology 136:4762-4768, 1995) demonstrating that the expression of cAMP-dependent nuclear transcription factor CREB (cAMP response element binding protein) is lost following ovulation in macaques has revealed a novel mechanism by which the cytoplasmic and nuclear actions the cA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5346
更新日期:1997-08-01 00:00:00
abstract::Recent observations suggest a role for interleukin-1 (IL-1), a polypeptide product of macrophage/monocytic cells, in the immune-mediated destruction of pancreatic islet beta-cells observed in type 1 diabetes. In this study, we investigated the effects of IL-1 on both alpha- and beta-cell secretory functions in rat isl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2393
更新日期:1988-06-01 00:00:00
abstract::Studies in the rat and rabbit indicate that facilitatory effects of neuropeptide Y (NPY) as well as norepinephrine (NE) on LH and LHRH release are dependent on the presence of the ovarian steroid estrogen. However, we have previously found the NE and an alpha-1-adrenergic agonist are both stimulatory to pulsatile LHRH...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-1144
更新日期:1991-02-01 00:00:00