Abstract:
:Basal or hypothalamic extract-induced ACTH or TSH secretory capacity as well as intracellular ACTH content were assessed in cultured rat adenohypophyseal cells after various pretreatments of the donor animals. Medium and intracellular ACTH or TSH were measured by radioimmunoassay. Ten-day to 2-month adrenalectomy enhanced, whereas dexamethasone pretreatment suppressed, both synthesis and secretion of ACTH in the adenohypophyseal cells. Dexamethasone may also impair the replication of the adenohypophyseal cells in vivo, as indicated by the smaller number of cells obtained per animal in the dexamethasone-treated group. No significant increase in TSH secretion was observed when adenohypophyseal cells were derived from rats fed propyl-thiouracil (PTU) for 3 weeks instead of from intact rats.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Yasuda N,Takebe K,Greer MAdoi
10.1210/endo-98-3-717subject
Has Abstractpub_date
1976-03-01 00:00:00pages
717-21issue
3eissn
0013-7227issn
1945-7170journal_volume
98pub_type
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