Abstract:
:The atrophy and hypofunction of the adrenal cortex following long-term pharmacologic glucocorticoid therapy is a major health problem necessitating chronic glucocorticoid replacement that often prolongs the ultimate return of endogenous adrenocortical function. Underlying this functional recovery is anatomic regeneration, the cellular and molecular mechanisms of which are poorly understood. Investigating the lineage contribution of cortical Sonic hedgehog (Shh)+ progenitor cells and the SHH-responsive capsular Gli1+ cells to the regenerating adrenal cortex, we observed a spatially and temporally bimodal contribution of both cell types to adrenocortical regeneration following cessation of glucocorticoid treatment. First, an early repopulation of the cortex is defined by a marked delamination and expansion of capsular Gli1+ cells, recapitulating the establishment of the capsular-cortical homeostatic niche during embryonic development. This rapid repopulation is promptly cleared from the cortical compartment only to be supplanted by repopulating cortical cells derived from the resident long-term-retained zona glomerulosa Shh+ progenitors. Pharmacologic and genetic dissection of SHH signaling further defines an SHH-dependent activation of WNT signaling that supports regeneration of the cortex following long-term glucocorticoid therapy. We define the signaling and lineage relationships that underlie the regeneration process.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Finco I,Lerario AM,Hammer GDdoi
10.1210/en.2017-03061subject
Has Abstractpub_date
2018-02-01 00:00:00pages
579-596issue
2eissn
0013-7227issn
1945-7170pii
4677346journal_volume
159pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Mediators of lymphocyte infiltration in inflammatory thyroid disease have yet to be identified. Here we examine the ability of IL-1beta to enhance the production of chemoattractants by human thyrocytes. Primary cultures, when treated with the cytokine, release T lymphocyte chemotactic activity. The effect of IL-1beta ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0199
更新日期:2003-07-01 00:00:00
abstract::The effects of estrogen on the synthesis of pituitary PRL mRNA and PRL and the release of PRL into plasma were investigated in male and female normal and hypogonadal (hpg) mice. Because the hpg mouse is totally deficient in hypothalamic GnRH, it provides an excellent model for investigating the effects of estrogen and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2422
更新日期:1986-12-01 00:00:00
abstract::Ovarian cancer in women is a complex and deadly disease, where the molecular events that initiate and control tumor formation remain poorly defined. Therefore, mouse models provide one approach for determining the mechanisms by which specific oncogenic factors cause ovarian surface epithelial cell and granulosa cell t...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2011-2121
更新日期:2012-04-01 00:00:00
abstract::Our previous studies have identified a role for annexin 1 (also called lipocortin 1) in the regulatory actions of glucocorticoids (GCs) on the release of PRL from the rat anterior pituitary gland. In the present study we used antisense and immunoneutralization strategies to extend this work. Exposure of rat anterior p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.6.7512
更新日期:2000-06-01 00:00:00
abstract::The estrogen-induced and -dependent Syrian hamster renal tumor is the most intensively studied model in estrogen carcinogenesis. Yet, it remains confounding that the kidney of this species behaves as an estrogen target tissue. As both reproductive and urinary systems arise from the same germinal ridge, we propose that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8355
更新日期:2001-09-01 00:00:00
abstract::Amplification of insulin secretion by cAMP is mediated by protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). Using selective activators, we determined how each effector influences the cytosolic free Ca(2+) concentration ([Ca(2+)]c) and insulin secretion in mouse islets. Alone PKA activator ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1247
更新日期:2014-09-01 00:00:00
abstract::The activity of the Na+/H+ exchanger was studied by measuring the effects of intracellular pH (pHi) and extracellular Na+ [(Na+)o] on pHi recovery and 22Na uptake in rat adipocytes. The resting pHi was acidified from 7.30 +/- 0.02 to 6.99 +/- 0.01 with nigericin in the absence of (Na+)o. pHi recovery induced by 30 mM ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.7.7789340
更新日期:1995-07-01 00:00:00
abstract::To study the localization of PRL in mammary epithelial cells (MEC) and to characterize PRL forms in serum, milk, and mammary tissue, two groups of lactating rats, a control group and a bromocriptine-treated group, were compared. In serum and milk from control rats, two forms of PRL (25 and 23 kDa) were detected by imm...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895370
更新日期:1996-11-01 00:00:00
abstract::We have studied the long-term effects of nicotinamide (NIC) on the synthesis of NO by insulin producing cells. NIC delays the formation of nitrite by interleukin (IL)-1 beta-(IL-1, 25 U/ml)-stimulated RINm5F cells, and previous exposure of cells to IL-1 for 15 h prevents this effect. The delay is associated with a lac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895350
更新日期:1996-11-01 00:00:00
abstract::Recent work has implicated stimulatory kisspeptin neurons in the arcuate nucleus (ARC) as important for seasonal changes in reproductive function in sheep, but earlier studies support a role for inhibitory A15 dopaminergic (DA) neurons in the suppression of GnRH (and LH) pulse frequency in the nonbreeding (anestrous) ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1611
更新日期:2012-12-01 00:00:00
abstract::A mitochondrial fraction prepared from homogenates of rat hypothalamic tissue was found by means of electron microscopy to be enriched with synaptosomes. The release of luteinizing hormone releasing hormone (LHRH) and thyrotropin releasing hormone (TRH) from this preparation was investigated. After incubation, the syn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-3-814
更新日期:1977-03-01 00:00:00
abstract::To study conversion of 3,5,3'-triiodothyroinine (T3) and 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (T2) in vitro, T3 or rT3 was incubated at pH 7.35 with homogenates of several rat tissues (liver, kidney, muscle, heart, ling, spleen, intestines, and brain) for 15 min at 37 C. The T2 generated during incub...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-4-1099
更新日期:1978-04-01 00:00:00
abstract::Within the preoptic region, nitric oxide (NO) production varies during the ovarian cycle and has the ability to impact hypothalamic reproductive function. One mechanism for the regulation of NO release mediated by estrogens during the estrous cycle includes physical association of the calcium-activated neuronal NO syn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0007
更新日期:2010-06-01 00:00:00
abstract::To elucidate the temporal profile of adaptive changes of the islets of Langerhans to the increased insulin demands of pregnancy, we have studied islet cell proliferation and insulin secretion during gestation in the rat. 5-Bromo-2'-deoxyuridine incorporation into dividing islet cells was significantly (P less than 0.0...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1537300
更新日期:1992-03-01 00:00:00
abstract::The effects of gamma-aminobutyric acid (GABA) on the spontaneous and TRH-induced TSH release were investigated in vitro on perifused rat pituitaries. The dynamic pattern of TSH release was measured in response to a 6-min pulse of TRH (10 nM) with or without GABA addition. GABA had no effect on spontaneous TSH release ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-3-980
更新日期:1987-09-01 00:00:00
abstract::Hereditary vitamin D-resistant rickets (HVDRR) is an autosomal recessive disease caused by mutations in the vitamin D receptor (VDR). We studied a young Saudi Arabian girl who exhibited the typical clinical features of HVDRR, but without alopecia. Analysis of her VDR gene revealed a homozygous T to C mutation in exon ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0080
更新日期:2004-11-01 00:00:00
abstract::Glucocorticoids and nonsteroidal antiinflammatory drugs (NSAIDs) are widely used for the treatment of inflammatory and immune diseases. Nitric oxide (NO) has a diversity of physiological functions, but its excess production has been implicated in the inflammatory process. The present study was designed to elucidate th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6718
更新日期:1999-05-01 00:00:00
abstract::Sulfonylureas are drugs widely used in the treatment of patients with type 2 diabetes mellitus. In addition to their pancreatic effect of stimulating insulin secretion, many studies suggest that sulfonylureas also have extrapancreatic actions. We have previously reported that gliclazide, a second-generation sulfonylur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0755
更新日期:2004-02-01 00:00:00
abstract::In the present studies we examined the regulation of insulin-like growth factor I (IGF-I) expression in porcine granulosa cells in vitro. Using Northern analysis and ribonuclease protection assays with exon-specific probes, we identified the IGF-I messenger RNA (mRNA) transcripts present in these cells under basal and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895330
更新日期:1996-11-01 00:00:00
abstract::The pattern of glucagon gene expression and the posttranslational processing of proglucagon was studied in the fetal and neonatal rat. Pancreatic immunoreactive glucagon (IRG) and glucagon-like immunoreactivity (GLI) were low in both fetal pancreas and intestine, respectively. Immediately after birth, pancreatic IRG r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2217
更新日期:1990-11-01 00:00:00
abstract::We studied the effects of vitamin D deficiency and its correction by vitamin D or calcium-lactose supplementation on vitamin D receptor (VDR) expression in skin keratinocytes, kidney, and duodenum of adult rats. VDR messenger RNA (mRNA) was assayed by Northern blot, and VDR protein was determined immunocytochemically....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5903
更新日期:1998-04-01 00:00:00
abstract::This study examined the requirement for 17 beta-estradiol (E) and relaxin (R) during the preparturient period (approximately the last 30 h of gestation) for normal parturition and pup survival in the rat. On the evening of day 21 of pregnancy, primiparous Sprague-Dawley rats were bilaterally ovariectomized or sham ope...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1958
更新日期:1988-05-01 00:00:00
abstract::Acidosis has been implicated in the pathogenesis of various metabolic bone diseases. Experimental acid ingestion is known to stimulate the release of bone mineral in humans and increase osteoclastic resorption surfaces in animals, but the mechanism has remained unclear. We have investigated the effect of medium pH on ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-1-119
更新日期:1986-07-01 00:00:00
abstract::Female rats were ovariectomized and either sham-pinealectomized or pinealectomized on day 24 of age and exposed to a photoperiod of 12 h light: 12 h dark. In vivo and in vitro measures of adrenocortical function were made at 6, 7, and 8 weeks of age (18, 25, and 32 days post-pinealectomy, respectively). Pinealectomy d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-1-20
更新日期:1976-01-01 00:00:00
abstract::T4-binding globulin (TBG), the principal carrier of thyroid hormone in serum, is a glycoprotein containing 20% carbohydrate. The importance of the carbohydrate moiety has been previously studied by enzymatic deglycosylation, which showed that deglycosylated TBG retains its original immunological an T4-binding properti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1614
更新日期:1986-04-01 00:00:00
abstract::Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of anterior pituitary hormones. The little mutation in mice is analogous to autosomal recessive, isolated, partial deficiency...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-5-1672
更新日期:1982-05-01 00:00:00
abstract::The importance of thyroid hormone (TH) in wound healing is not well understood. To gain insight, we evaluated the impact of TH deficiency on wound-healing genes in cultured keratinocytes. By RT-PCR, keratin 6a (K6a) and 16 (K16) gene expression in TH replete cells was 3.8- (P < 0.005) and 1.9-fold (P < 0.05) greater, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1696
更新日期:2004-05-01 00:00:00
abstract::Diabetes is associated with increased generation of cytokines and tissue inflammation, but it is unclear how increased cytokine synthesis is causally related to the development of diabetic complications. Here, we report that exposure to high (25 mm) glucose, but not iso-osmotic concentrations of mannitol or 3-methyl g...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0512
更新日期:2007-09-01 00:00:00
abstract::Glucocorticoid (Gc) excess, from endogenous overproduction in disorders of the hypothalamic-pituitary-adrenal axis or exogenous medical therapy, is recognized to cause adverse metabolic side effects. The Gc receptor (GR) is widely expressed throughout the body, including brain regions such as the hypothalamus. However...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1571
更新日期:2016-11-01 00:00:00
abstract::Uterine estrogen receptor (ER) and ER mRNA were measured in cycling and ovariectomized (OVX) estrogen-treated mice to probe the physiological regulation of the intracellular distribution and biosynthesis of ER. On proestrus, when plasma estradiol (E2) levels are highest, the cell nuclear ER concentration was 2.4-fold ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547720
更新日期:1992-04-01 00:00:00