Parathyroid hormone increases activating transcription factor 4 expression and activity in osteoblasts: requirement for osteocalcin gene expression.


:PTH is an important peptide hormone regulator of calcium homeostasis and osteoblast function. However, its mechanism of action in osteoblasts is poorly understood. Our previous study demonstrated that PTH activates mouse osteocalcin (Ocn) gene 2 promoter through the osteoblast-specific element 1 site, a recently identified activating transcription factor-4 (ATF4) -binding element. In the present study, we examined effects of PTH on ATF4 expression and activity as well as the requirement for ATF4 in the regulation of Ocn by PTH. Results show that PTH elevated levels of ATF4 mRNA and protein in a dose- and time-dependent manner. This PTH regulation requires transcriptional activity but not de novo protein synthesis. PTH also increased binding of nuclear extracts to osteoblast-specific element 1 DNA. PTH stimulated ATF4-dependent transcriptional activity mainly through protein kinase A with a lesser requirement for protein kinase C and MAPK/ERK pathways. Lastly, PTH stimulation of Ocn expression was lost by small interfering RNA down-regulation of ATF4 in MC-4 cells and Atf4(-/-) bone marrow stromal cells. Collectively, these studies for the first time demonstrate that PTH increases ATF4 expression and activity and that ATF4 is required for PTH induction of Ocn expression in osteoblasts.






Yu S,Franceschi RT,Luo M,Zhang X,Jiang D,Lai Y,Jiang Y,Zhang J,Xiao G




Has Abstract


2008-04-01 00:00:00














  • Hypothalamic interleukin-1 beta and tumor necrosis factor-alpha, but not interleukin-6, mediate the endotoxin-induced suppression of the reproductive axis in rats.

    abstract::It is well established that endotoxemia disrupts reproductive capability, and several proinflammatory cytokines, especially IL-1 beta, IL-6, and TNF-alpha in the brain, have been implicated in this endocrine aberration. However, no previous study has directly compared the effects of the three major proinflammatory cyt...


    pub_type: 杂志文章


    authors: Watanobe H,Hayakawa Y

    更新日期:2003-11-01 00:00:00

  • Evidence that alpha-calcitonin gene-related peptide is a neurohormone that controls systemic lipid availability and utilization.

    abstract::Alpha-calcitonin gene-related peptide (alphaCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary physiological role has thus far remained obscure. Increased lipid content, particularly in skeletal muscle and liver, is ...


    pub_type: 杂志文章


    authors: Danaher RN,Loomes KM,Leonard BL,Whiting L,Hay DL,Xu LY,Kraegen EW,Phillips AR,Cooper GJ

    更新日期:2008-01-01 00:00:00

  • Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures.

    abstract::Insulin-like growth factors I and II (IGF-I and -II) are polypeptides secreted by skeletal cells and are considered regulators of bone formation. IGF-I and -II were studied for their effects on collagen synthesis and degradation in cultures of intact fetal rat calvariae and on type I collagen transcript levels in oste...


    pub_type: 杂志文章


    authors: McCarthy TL,Centrella M,Canalis E

    更新日期:1989-01-01 00:00:00

  • Growth factors regulate the synthesis of insulin-like growth factor-I in bone cell cultures.

    abstract::Insulin-like growth factor-I (IGF-I), a prevalent growth factor secreted by bone cells, has important effects on bone remodeling. Hormones are known to regulate the synthesis of skeletal IGF-I, but there is limited information about the actions of growth factors on IGF-I synthesis. We tested the effects of basic fibro...


    pub_type: 杂志文章


    authors: Canalis E,Pash J,Gabbitas B,Rydziel S,Varghese S

    更新日期:1993-07-01 00:00:00

  • Expression of messenger ribonucleic acid encoding tissue inhibitor of metalloproteinases-2 within ovine follicles and corpora lutea.

    abstract::Metalloproteinase inhibitors, such as tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and -2), play a key role in the regulation of metalloproteinases that modify extracellular matrix composition. Although expression of TIMP-1 within ovarian tissues has been well characterized, little information is available ...


    pub_type: 杂志文章


    authors: Smith GW,McCrone S,Petersen SL,Smith MF

    更新日期:1995-02-01 00:00:00

  • Characterization of thyrotropin-induced increase in iodothyronine monodeiodinating activity in mice.

    abstract::To further characterize the effect of TSH administration on thyroid iodothyronine monodeiodinating activity, we have evaluated the in vitro conversion of T4 to T3 (outer ring deiodination) and T3 to 3,3'-diiodothyronine (T2; inner ring deiodination) by mouse thyroid, liver, and kidney homogenates, comparing tissues fr...


    pub_type: 杂志文章


    authors: Wu SY,Reggio R,Florsheim WH

    更新日期:1985-03-01 00:00:00

  • Progesterone antagonizes the permissive action of estradiol on tumor necrosis factor-alpha-induced apoptosis of anterior pituitary cells.

    abstract::We previously reported that TNF-alpha-induced apoptosis of lactotropes is estrogen dependent and predominant at proestrus. Here we observed that TNF-alpha (50 ng/ml) failed to induce apoptosis of anterior pituitary cells from ovariectomized rats cultured in the presence of progesterone (10(-6) m). However, progesteron...


    pub_type: 杂志文章


    authors: Candolfi M,Jaita G,Zaldivar V,Zárate S,Ferrari L,Pisera D,Castro MG,Seilicovich A

    更新日期:2005-02-01 00:00:00

  • Nuclear factor-kappaB activates transcription of the androgen receptor gene in Sertoli cells isolated from testes of adult rats.

    abstract::The androgen receptor (AR) in Sertoli cells mediates the actions of testosterone on spermatogenesis. However, the transcription factors responsible for AR gene regulation in Sertoli cells remain unknown. In this study, we determined that nuclear factor-kappaB (NF-kappaB) regulates transcription of AR in primary cultur...


    pub_type: 杂志文章


    authors: Zhang L,Charron M,Wright WW,Chatterjee B,Song CS,Roy AK,Brown TR

    更新日期:2004-02-01 00:00:00

  • Moderate daily exercise activates metabolic flexibility to prevent prenatally induced obesity.

    abstract::Obesity and its associated comorbidities are of major worldwide concern. It is now recognized that there are a number of metabolically distinct pathways of obesity development. The present paper investigates the effect of moderate daily exercise on the underlying mechanisms of one such pathway to obesity, through inte...


    pub_type: 杂志文章


    authors: Miles JL,Huber K,Thompson NM,Davison M,Breier BH

    更新日期:2009-01-01 00:00:00

  • Endogenous peroxisome proliferator-activated receptor-gamma augments fatty acid uptake in oxidative muscle.

    abstract::In the setting of insulin resistance, agonists of peroxisome proliferator-activated receptor (PPAR)-gamma restore insulin action in muscle and promote lipid redistribution. Mice with muscle-specific knockout of PPARgamma (MuPPARgammaKO) develop excess adiposity, despite reduced food intake and normal glucose disposal ...


    pub_type: 杂志文章


    authors: Norris AW,Hirshman MF,Yao J,Jessen N,Musi N,Chen L,Sivitz WI,Goodyear LJ,Kahn CR

    更新日期:2008-11-01 00:00:00

  • S14: insights from knockout mice.

    abstract::Spot 14 (S14) is a protein whose mRNA is rapidly up-regulated by lipogenic stimuli including thyroid hormone and a high-carbohydrate diet. Previous investigation into the role of S14 suggested that it is involved in de novo lipogenesis. Knockout of the gene in mice has given further support to this hypothesis. The lac...


    pub_type: 杂志文章,评审


    authors: LaFave LT,Augustin LB,Mariash CN

    更新日期:2006-09-01 00:00:00

  • Estrogen receptor-immunoreactive glia, endothelia, and ependyma in guinea pig preoptic area and median eminence: electron microscopy.

    abstract::The presence of estrogen receptors (ERs) in nonneural cells in brain, including glia, ependyma, and endothelia, has not previously been documented with electron microscopy. This study employed immunocytochemistry to investigate whether ER immunoreactivity (ER-ir) is present in glial, ependymal, or endothelial cells in...


    pub_type: 杂志文章


    authors: Langub MC Jr,Watson RE Jr

    更新日期:1992-01-01 00:00:00

  • Glucocorticoid stimulation of growth hormone messenger ribonucleic acid levels in GH3 cells is inhibited by calcium but not by somatostatin.

    abstract::Aside from studies of a possible synergism between T3 and glucocorticoids in regulating GH mRNA, there have been few previous studies of the modulation by hormones and other factors of glucocorticoid hormone regulation of pituitary cell GH gene expression. We have employed a serum-free medium containing no exogenously...


    pub_type: 杂志文章


    authors: Gick GG,Bancroft C

    更新日期:1987-05-01 00:00:00

  • Mouse placental lactogen-I: RIA and gestational profile in maternal serum.

    abstract::A RIA for mouse placental lactogen-I (mPL-I) was developed using recombinant mPL-I as the standard, radioligand, and antigen for antiserum production. Displacement curves for dilutions of serum and placental extracts from pregnant mice were parallel to the recombinant mPL-I standard curve. Serum from male and nonpregn...


    pub_type: 杂志文章


    authors: Ogren L,Southard JN,Colosi P,Linzer DI,Talamantes F

    更新日期:1989-11-01 00:00:00

  • Annexin 1 (lipocortin 1) mediates the glucocorticoid inhibition of cyclic adenosine 3',5'-monophosphate-stimulated prolactin secretion.

    abstract::Our previous studies have identified a role for annexin 1 (also called lipocortin 1) in the regulatory actions of glucocorticoids (GCs) on the release of PRL from the rat anterior pituitary gland. In the present study we used antisense and immunoneutralization strategies to extend this work. Exposure of rat anterior p...


    pub_type: 杂志文章


    authors: Taylor AD,Philip JG,John CD,Cover PO,Morris JF,Flower RJ,Buckingham JC

    更新日期:2000-06-01 00:00:00

  • The mechanism of ICI 164,384 antiestrogenicity involves rapid loss of estrogen receptor in uterine tissue.

    abstract::The antiestrogen ICI 164,384 (ICI) binds the estrogen receptor (ER) with approximately 20% the affinity of estradiol, but without the partial agonistic effects caused by tamoxifen. Investigations into the mechanism of ICI action have used ER molecules expressed in vitro to examine the binding of ER to ICI and the capa...


    pub_type: 杂志文章


    authors: Gibson MK,Nemmers LA,Beckman WC Jr,Davis VL,Curtis SW,Korach KS

    更新日期:1991-10-01 00:00:00

  • Cytokine-activated endothelium recruits osteoclast precursors.

    abstract::Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recruitment to sites of ...


    pub_type: 杂志文章


    authors: McGowan NW,Walker EJ,Macpherson H,Ralston SH,Helfrich MH

    更新日期:2001-04-01 00:00:00

  • Induction of CXCL1 by extracellular matrix and autocrine enhancement by interleukin-1 in rat pancreatic beta-cells.

    abstract::As we showed previously, the extracellular matrix (ECM) derived from rat bladder carcinoma cells (804G-ECM) has positive effects on rat primary beta-cell function and survival in vitro. The aim of this study was to define beta-cell genes induced by this ECM with a specific focus on cytokines. Analysis of differential ...


    pub_type: 杂志文章


    authors: Ribaux P,Ehses JA,Lin-Marq N,Carrozzino F,Böni-Schnetzler M,Hammar E,Irminger JC,Donath MY,Halban PA

    更新日期:2007-11-01 00:00:00

  • Intermittent Fasting Promotes Fat Loss With Lean Mass Retention, Increased Hypothalamic Norepinephrine Content, and Increased Neuropeptide Y Gene Expression in Diet-Induced Obese Male Mice.

    abstract::Clinical studies indicate alternate-day, intermittent fasting (IMF) protocols result in meaningful weight loss in obese individuals. To further understand the mechanisms sustaining weight loss by IMF, we investigated the metabolic and neural alterations of IMF in obese mice. Male C57/BL6 mice were fed a high-fat diet ...


    pub_type: 杂志文章


    authors: Gotthardt JD,Verpeut JL,Yeomans BL,Yang JA,Yasrebi A,Roepke TA,Bello NT

    更新日期:2016-02-01 00:00:00

  • Impaired beta-cell function and inadequate compensatory increases in beta-cell mass after intrauterine growth restriction in sheep.

    abstract::Poor growth before birth increases the risk of non-insulin-dependent diabetes mellitus (NIDDM) and impairs insulin secretion relative to sensitivity. We investigated the effects of intrauterine growth restriction in sheep on insulin secretion, beta-cell mass, and function from before birth to young adulthood and its m...


    pub_type: 杂志文章


    authors: Gatford KL,Mohammad SN,Harland ML,De Blasio MJ,Fowden AL,Robinson JS,Owens JA

    更新日期:2008-10-01 00:00:00

  • Genes associated with membrane-initiated signaling of estrogen and energy homeostasis.

    abstract::During the reproductive cycle, fluctuations in circulating estrogens affect multiple homeostatic systems controlled by hypothalamic neurons. Two of these neuronal populations are arcuate proopiomelanocortin and neuropeptide Y neurons, which control energy homeostasis and feeding. Estradiol modulates these neurons eith...


    pub_type: 杂志文章


    authors: Roepke TA,Xue C,Bosch MA,Scanlan TS,Kelly MJ,Rønnekleiv OK

    更新日期:2008-12-01 00:00:00

  • Effect of thyroid status and paraventricular area lesions on the release of thyrotropin-releasing hormone and catecholamines into hypophysial portal blood.

    abstract::TRH is a potent stimulator of pituitary TSH release, but its function in the physiological regulation of thyroid activity is still controversial. The purpose of the present study was to investigate TRH and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing p...


    pub_type: 杂志文章


    authors: Rondeel JM,de Greef WJ,van der Schoot P,Karels B,Klootwijk W,Visser TJ

    更新日期:1988-07-01 00:00:00

  • Phenotypic plasticity of the ovarian surface epithelium: TGF-beta 1 induction of epithelial to mesenchymal transition (EMT) in vitro.

    abstract::Ovarian surface epithelium (OSE) is the most conceivable cell origin of epithelial ovarian carcinomas. Unlike many other epithelial tumors, the precancerous lesion acquires expression of epithelial markers, e.g. E-cadherin and claudins, suggesting that OSE cells undergo mesenchymal to epithelial transition (MET) durin...


    pub_type: 杂志文章


    authors: Zhu Y,Nilsson M,Sundfeldt K

    更新日期:2010-11-01 00:00:00

  • Differential regulation of IGF-binding protein gene expression by cAMP in rat C6 glioma cells.

    abstract::We previously reported that cAMP inhibits autocrine IGF-I gene expression in rat C6 glioma cells. In this study we examined the influence of cAMP on IGF-binding protein gene expression in C6 cells. cAMP potently inhibited IGF-binding protein-3 mRNA and, to a lesser extent, IGF-binding protein-4 mRNA and transiently st...


    pub_type: 杂志文章


    authors: Wang L,Ma X,Yeh LC,Adamo ML

    更新日期:2001-09-01 00:00:00

  • Delivering the code: polyplex carriers for deoxyribonucleic acid and ribonucleic acid interference therapies.

    abstract::Nucleic acid-based therapies offer great potential for treatment of a variety of diseases including cancer by modulating protein expression with DNA or small interfering RNA. However, realization of their full therapeutic potential is currently limited due to an inability to reach the target site in an active form. Id...


    pub_type: 杂志文章,评审


    authors: Christie RJ,Nishiyama N,Kataoka K

    更新日期:2010-02-01 00:00:00

  • Renal metabolism of gut glucagon-like immunoreactivity.

    abstract::To examine the role of the kidney in the catabolism of gut glucagon-like immunoreactivity (GLI), we compared the plasma GLI responses of normal and nephrectomized dogs given intraduodenal glucose loads and studied the clearance of gut GLI by the isolated perfused rat kidney. Both basal and postload plasma samples were...


    pub_type: 杂志文章


    authors: Rovira A,López-Novoa JM,Zubiaur M,Matesanz R,Ghiglione M,Pascual JM,Valverde I

    更新日期:1982-06-01 00:00:00

  • Glucocorticoids differentially modulate insulin-mediated protein and glycogen synthetic signaling downstream of protein kinase B in rat myocardium.

    abstract::Insulin and protein kinase B (or Akt) play critical roles in cardiomyocytic growth and survival. High concentrations of glucocorticoids antagonize insulin's action. To examine whether endogenous glucocorticoids modulate insulin's effect on Akt signaling in the protein and glycogen synthetic pathways in myocardium, we ...


    pub_type: 杂志文章


    authors: Wu Y,Barrett EJ,Long W,Liu Z

    更新日期:2004-03-01 00:00:00

  • Long-Term Neonatal Estrogen Exposure Causes Irreversible Inhibition of LH Pulses by Suppressing Arcuate Kisspeptin Expression via Estrogen Receptors α and β in Female Rodents.

    abstract::Exposure to estrogen during the developmental period causes reproductive dysfunction in mammals, because the developing brain is highly sensitive to estrogens. In the present study, we report that long-term exposure to supraphysiological doses of estrogen during the neonatal critical period causes irreversible suppres...


    pub_type: 杂志文章


    authors: Minabe S,Ieda N,Watanabe Y,Inoue N,Uenoyama Y,Maeda KI,Tsukamura H

    更新日期:2017-09-01 00:00:00

  • Insulin/insulin-like growth factor-I hybrid receptors with high affinity for insulin are developmentally regulated during neurogenesis.

    abstract::The extensive colocalization of insulin receptor (IR) and insulin-like growth factor-I receptor (IGFR) messenger RNAs during central nervous system development, together with the effects of insulin and IGF-I in neurogenesis, raises the question of how stage- and factor-specific signaling occurs. Thus, it is necessary ...


    pub_type: 杂志文章


    authors: García-de Lacoba M,Alarcón C,de la Rosa EJ,de Pablo F

    更新日期:1999-01-01 00:00:00

  • Age-associated changes in nuclear binding of rat uterine estradiol receptor complexes.

    abstract::Nuclear binding of cytoplasmic estrogen receptors was measured in an in vitro cell-free system, using various mixtures of cytosols and nuclei from uteri of mature (6-9 month old) and senescent (24-25 month old) Wistar rats. Both nuclei and cytoplasmic receptors from senescent uteri were 25-35% less efficient in suppor...


    pub_type: 杂志文章


    authors: Chuknyiska RS,Haji M,Foote RH,Roth GS

    更新日期:1985-02-01 00:00:00