Age-related changes in pulsatile luteinizing hormone release precede the transition to estrous acyclicity and depend upon estrous cycle history.

abstract：:In the bovine ovary, thecal cells are the only cell type capable of expressing the CYP17 gene in response to LH. With the onset of ovulation and luteinization in the cow, there is complete loss of P450c17alpha expression. To characterize the molecular mechanisms involved in tissue-specific regulation of the CYP17 gene...

journal_title：Endocrinology

authors： Borroni R,Liu Z,Simpson ER,Hinshelwood MM

更新日期：1997-05-01 00:00:00

abstract：:Plasma chromogranin-A (CgA) concentrations correlate with severe cardiovascular diseases, whereas CgA-derived vasostatin-I and catestatin elicit cardiosuppression via an antiadrenergic/nitric oxide-cGMP mediated mechanism. Whether these phenomena are related is unknown. We here investigated whether and to what extent ...

journal_title：Endocrinology

authors： Pasqua T,Corti A,Gentile S,Pochini L,Bianco M,Metz-Boutigue MH,Cerra MC,Tota B,Angelone T

更新日期：2013-09-01 00:00:00

abstract：:A chemiluminescent dot blot assay has been developed by our laboratory for rapid determinations of IGF-I in serum-free conditioned media (CM) collected from cultured cells. In contrast to IGF-I radioimmunoassays (RIAs), the IGF binding proteins (IGFBPs) did not interfere with the dot blot assay and did not require the...

journal_title：Endocrinology

authors： De León DD,Asmerom Y

更新日期：1997-05-01 00:00:00

abstract：:Nitric oxide is a ubiquitous estrogen-regulated signaling molecule that has been implicated in the regulation of bone maturation and remodeling. To better understand the role that bone-cell-secreted nitric oxide plays in ovariectomy-induced modifications of bone turnover, we examined the expression of endothelial NO s...

journal_title：Endocrinology

authors： Grassi F,Fan X,Rahnert J,Weitzmann MN,Pacifici R,Nanes MS,Rubin J

更新日期：2006-09-01 00:00:00

abstract：:Kisspeptins are hypothalamic neuropeptides encoded by KISS1 and recently described as major regulators of GnRH release from hypothalamic neurons. Although 17beta-estradiol (E2)-induced up-regulation of KISS1 expression has been documented in anteroventral periventricular nucleus neurons, E2 down-regulates KISS1 expres...

journal_title：Endocrinology

authors： Huijbregts L,de Roux N

更新日期：2010-08-01 00:00:00

abstract：:Swine granulosa cells cultured under serum-free conditions in vitro exhibit significant responsivity to the stimulatory actions of estradiol (E2) and FSH or LH. Under these conditions, granulosa cells harvested from either immature or mature Graafian follicles synthesized significantly increased quantities of progeste...

journal_title：Endocrinology

authors： Veldhuis JD,Gwynne JT,Strauss JF 3rd,Demers LM

更新日期：1984-06-01 00:00:00

abstract：:Although polychlorinated biphenyls (PCBs) have been shown to accumulate in the adrenal, little is known about the effects of these endocrine disruptors on adrenal steroidogenesis. Our previous studies showed that high concentrations of PCB126 stimulated CYP11B1 and CYP11B2 mRNA expression and consequently raised corti...

journal_title：Endocrinology

authors： Lin TC,Chien SC,Hsu PC,Li LA

更新日期：2006-03-01 00:00:00

abstract：:CRF activity of synthetic vasopressins and pitressin was studied in an in vitro system of cultured rat adenohypophyseal cells using direct measurement of ACTH by radioimmunoassay. Pitressin (posterior pituitary extract) induced a dose-related secretion of ACTH whereas synthetic arginine or lysine vasopressin were devo...

journal_title：Endocrinology

authors： Yasuda N,Greer MA

更新日期：1976-04-01 00:00:00

abstract：:Uterine epithelial cells (UEC) isolated from 6-week-old CF-1 mice were immortalized using retroviral-mediated transfection of SV40 large T-antigen. One line, WEG-1, retained epithelial morphology and reacted with antibodies to cytokeratins 18, 19, laminin, fibronectin, and beta-catenin. In addition, WEG-1 cells displa...

journal_title：Endocrinology

authors： Wegner CC,Cherington V,Clemens JW,Jacobs AL,Julian J,Surveyor GA,Bell EC,Carson DD

更新日期：1996-01-01 00:00:00

abstract：:The DHCR24 gene encodes an enzyme catalyzing the last step of cholesterol biosynthesis, the conversion of desmosterol to cholesterol. To elucidate the physiological significance of cholesterol biosynthesis in mammalian cells, we investigated proliferation of mouse embryonic fibroblasts (MEFs) prepared from DHCR24(-/-)...

journal_title：Endocrinology

authors： Lu X,Kambe F,Cao X,Yoshida T,Ohmori S,Murakami K,Kaji T,Ishii T,Zadworny D,Seo H

更新日期：2006-06-01 00:00:00

abstract：:Dispersed Leydig cells were prepared from pig testes and purified in a discontinuous Percoll gradient. About 95% of these cells stained for 3 beta-hydroxysteroid dehydrogenase. The cells were cultured in a chemically defined medium. Testosterone production was low (2 +/- 0.4 ng/10(6) cells/day) under basal conditions,...

journal_title：Endocrinology

authors： Benahmed M,Reventos J,Saez JM

更新日期：1983-06-01 00:00:00

abstract：:The regulation of insulin-like growth factor binding protein (IGFBP)-4 proteolytic degradation by insulin-like growth factors (IGFs) has been largely studied in vitro, but not in vivo. The aim of this study was to investigate the involvement of IGFs, IGFBP-2, IGFBP-3, and IGFBP-3 proteolytic fragments in the regulatio...

journal_title：Endocrinology

authors： Mazerbourg S,Zapf J,Bar RS,Brigstock DR,Lalou C,Binoux M,Monget P

更新日期：1999-09-01 00:00:00

abstract：:Traditionally, it has been thought that the bioavailable fraction of circulating serum hormones, i.e. that which is available for cellular uptake and is physiologically active, is limited to the free (nonprotein bound) hormone. However, recent evidence, based on acute organ uptake of labeled hormone, suggests that the...

journal_title：Endocrinology

authors： Glass AR,Vigersky RA,Rajatanavin R,Pardridge W,Smallridge RC,Wartofsky L,Burman KD

更新日期：1984-05-01 00:00:00

abstract：:The SMA1-mouse is a novel ethyl-nitroso-urea (ENU)-induced mouse mutant that carries an a-->g missense mutation in exon 5 of the GH gene, which translates to a D167G amino acid exchange in the mature protein. Mice carrying the mutation are characterized by dwarfism, predominantly due to the reduction (sma1/+) or absen...

journal_title：Endocrinology

authors： Meyer CW,Korthaus D,Jagla W,Cornali E,Grosse J,Fuchs H,Klingenspor M,Roemheld S,Tschöp M,Heldmaier G,De Angelis MH,Nehls M

更新日期：2004-05-01 00:00:00

abstract：:11 beta-Hydroxysteroid dehydrogenase (11 beta HSI) is an enzyme complex responsible for the conversion of hormonally active cortisol to inactive cortisone; two isoforms of the enzyme have been cloned and characterized. Clinical observations from patients with the hypertensive syndrome apparent mineralocorticoid excess...

journal_title：Endocrinology

authors： Shimojo M,Ricketts ML,Petrelli MD,Moradi P,Johnson GD,Bradwell AR,Hewison M,Howie AJ,Stewart PM

更新日期：1997-03-01 00:00:00

abstract：:To probe the possible role of endogenous opiates in Sertoli cell proliferation during testicular development, the effect of interfering with beta-endorphin action either in vivo or in vitro was determined. The percent of Sertoli cells dividing was measured with quantitative autoradiography in [methyl 3H]-thymidine-exp...

journal_title：Endocrinology

authors： Orth JM

更新日期：1986-10-01 00:00:00

abstract：:Open pig thyroid follicles in which the apical surface of the follicle cells is in direct contact with the incubation medium were used to study the effect of stimulated exocytosis and stimulated H2O2 generation on the iodination of protein in the incubation medium. In previous studies on this system of follicles we ha...

journal_title：Endocrinology

authors： Björkman U,Ekholm R

更新日期：1988-02-01 00:00:00

abstract：:The metabolic consequences of visceral obesity have been associated with amplification of glucocorticoid action by 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in adipose tissue. This study aimed to assess in a rat model of diet-induced obesity the effects of pharmacological 11beta-HSD1 inhibition on the m...

journal_title：Endocrinology

authors： Berthiaume M,Laplante M,Festuccia W,Gélinas Y,Poulin S,Lalonde J,Joanisse DR,Thieringer R,Deshaies Y

更新日期：2007-05-01 00:00:00

abstract：:Nonalcoholic fatty liver disease (NAFLD) is the most frequent chronic liver disease in the United States and is strongly associated with hepatic insulin resistance. We examined whether the thyroid hormone receptor-α (Thra) would be a potential therapeutic target to prevent diet-induced NAFLD and insulin resistance. Fo...

journal_title：Endocrinology

authors： Jornayvaz FR,Lee HY,Jurczak MJ,Alves TC,Guebre-Egziabher F,Guigni BA,Zhang D,Samuel VT,Silva JE,Shulman GI

更新日期：2012-02-01 00:00:00

abstract：:Previous studies have shown that mice missing gp130, the common receptor subunit for many cytokines, die at or before birth with multiple skeletal abnormalities. Furthermore, interactions between PTH and gp130 signaling have suggested that gp130 signaling might influence calcium homeostasis. We, therefore, examined th...

journal_title：Endocrinology

authors： Shin HI,Divieti P,Sims NA,Kobayashi T,Miao D,Karaplis AC,Baron R,Bringhurst R,Kronenberg HM

更新日期：2004-03-01 00:00:00

abstract：:Immunoreactive neurophysin (Np) is demonstrated by the immunoperoxidase technique not only within neurones of the classical hypothalamo-neurohypophyseal system, but also in extrahypothalamic perikarya and fibers. Np-positive perikarya are found in the triangular nucleus of the septum. Np fibers join the stria terminal...

journal_title：Endocrinology

authors： Sofroniew MV,Weindl A

更新日期：1978-01-01 00:00:00

abstract：:The central characteristic of uterine fibroids is excessive deposition of extracellular matrix (ECM), which contributes to fibroid growth and bulk-type symptoms. Despite this, very little is known about patterns of ECM protein expression in fibroids and whether these are influenced by the most common genetic anomalies...

journal_title：Endocrinology

authors： Jamaluddin MFB,Ko YA,Kumar M,Brown Y,Bajwa P,Nagendra PB,Skerrett-Byrne DA,Hondermarck H,Baker MA,Dun MD,Scott RJ,Nahar P,Tanwar PS

更新日期：2018-02-01 00:00:00

abstract：:Neurons that produce kisspeptin play a critical role in reproduction. However, understanding the molecular physiology of kisspeptin neurons has been limited by the lack of an in vivo marker for those cells. Here, we report the development of a Kiss1-CreGFP knockin mouse, wherein the endogenous Kiss1 promoter directs t...

journal_title：Endocrinology

authors： Gottsch ML,Popa SM,Lawhorn JK,Qiu J,Tonsfeldt KJ,Bosch MA,Kelly MJ,Rønnekleiv OK,Sanz E,McKnight GS,Clifton DK,Palmiter RD,Steiner RA

更新日期：2011-11-01 00:00:00

abstract：:Estrogens are essential hormones for the regulation of fertility. Cellular responses to estrogens are mediated by estrogen receptor α (ESR1) and estrogen receptor β (ESR2). In mouse and rat models, disruption of Esr1 causes infertility in both males and females. However, the role of ESR2 in reproductive function remai...

journal_title：Endocrinology

authors： Rumi MAK,Singh P,Roby KF,Zhao X,Iqbal K,Ratri A,Lei T,Cui W,Borosha S,Dhakal P,Kubota K,Chakraborty D,Vivian JL,Wolfe MW,Soares MJ

更新日期：2017-07-01 00:00:00

abstract：:Gamma-aminobutyric acid (GABA) is one of the most important neurotransmitters that regulate the excitability of GnRH neurons. Numerous studies have shown that GABA activates Cl(-) currents in GnRH neurons, and these effects are antagonized by GABA(A) receptor antagonists. The GABA(B) receptor is a heterodimer composed...

journal_title：Endocrinology

authors： Zhang C,Bosch MA,Rønnekleiv OK,Kelly MJ

更新日期：2009-05-01 00:00:00

abstract：:We have previously described FSH receptor-mediated influx of 45Ca++ in cultured Sertoli cells from immature rats and receptor-enriched proteoliposomes via activation of voltage-sensitive and voltage-independent calcium channels. We have further shown that this effect of FSH does not require cholera toxin- or pertussis...

journal_title：Endocrinology

authors： Grasso P,Santa-Coloma TA,Reichert LE Jr

更新日期：1991-06-01 00:00:00

abstract：:The antiestrogen ICI 164,384 (ICI) binds the estrogen receptor (ER) with approximately 20% the affinity of estradiol, but without the partial agonistic effects caused by tamoxifen. Investigations into the mechanism of ICI action have used ER molecules expressed in vitro to examine the binding of ER to ICI and the capa...

journal_title：Endocrinology

authors： Gibson MK,Nemmers LA,Beckman WC Jr,Davis VL,Curtis SW,Korach KS

更新日期：1991-10-01 00:00:00

abstract：:Adrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal...

journal_title：Endocrinology

authors： Wong LY,Cheung BM,Li YY,Tang F

更新日期：2005-03-01 00:00:00

abstract：:In the rat a GH-binding protein (GHBP) exists that is derived from the GH receptor gene by an alternative messenger RNA splicing mechanism such that the transmembrane and intracellular domains of the GH receptor are replaced by a hydrophilic carboxy terminus. Previous immunohistochemical studies detailing the localiza...

journal_title：Endocrinology

authors： Lobie PE,García-Aragón J,Wang BS,Baumbach WR,Waters MJ

更新日期：1992-05-01 00:00:00

abstract：:From the MtTF4 tumor of rat pituitary origin we established the F4Z2 cell line whose growth is stimulated by 17 beta-estradiol (E2). Taking E2 actions as references we investigated actions of other effectors on the proliferation, protein, and insulin growth factor-I (IGF-I) secretions of F4F2 cells. Dexamethasone (Dex...

journal_title：Endocrinology

authors： Zhou-Li F,Joly-Pharaboz MO,Bouillard B,Albaladejo V,Nicolas B,Andre J

更新日期：1991-06-01 00:00:00