Abstract:
:Steroid receptors, including estrogen receptor (ER) and progesterone receptors (PR), form associations with heat shock proteins (Hsps). Dissociation of Hsps activates PR, whereas retention of Hsp90 in vitro stimulates ER. Progesterone and estrogen, interacting with their receptors, regulate myometrial contractility throughout pregnancy and during parturition. We hypothesize that uterine ER and PR changes concurrent with changes in Hsp90 and -70 abundance could alter uterine function. We quantified changes in Hsp90 and -70 messenger RNA (mRNA) abundance in pregnant sheep myometrium, endometrium, and fetal placenta during glucocorticoid-induced preterm and spontaneous labor. The effects pf estradiol and progesterone on Hsp90 and -70 mRNA in myometrium and endometrium were examined in ovariectomized nonpregnant ewes. Hsp90 and -70 mRNA distribution was evaluated by in situ hybridization in myometrium and endometrium. Dramatic tissue-specific increases in Hsp90 and -70 mRNA were observed in myometrium and endometrium (P < 0.05) during spontaneous and glucocorticoid-induced labor. Hsp90 and -70 mRNA localized in myometrial, arterial smooth muscle, and endometrial gland epithelial cells. Estradiol increased Hsp90 and -70 mRNA in myometrium and endometrium of nonpregnant ewes. Progesterone did not affect Hsp90 and -70 mRNA abundance, but inhibited the estradiol-stimulated increase. These data support our hypothesis that at term, increased abundance of Hsp90 and -70 may inhibit uterine PR and stimulate ER function in uterine tissues. Similar changes, if present, would be of importance in species showing no progesterone withdrawal before labor, such as primates, including pregnant women.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Wu WX,Derks JB,Zhang Q,Nathanielsz PWdoi
10.1210/endo.137.12.8940400subject
Has Abstractpub_date
1996-12-01 00:00:00pages
5685-93issue
12eissn
0013-7227issn
1945-7170journal_volume
137pub_type
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