Abstract:
:Hyp mice, the murine homolog of human X-linked hypophosphatemia (XLH), are characterized in part by elevated renal loss of phosphate (Pi) and hypophosphatemia. The lesion responsible for this syndrome is believed to be defective Na+ gradient-dependent phosphate transport across renal brush border membranes (BBM). It has been demonstrated that thyroid hormones can stimulate Pi uptake by rat BBM vesicles (BBMV). The purpose of this current study was to determine whether thyroid hormones could increase renal BBMV transport of Pi and improve the renal conservation of Pi in Hyp mice. Hyp mice were treated with either vehicle (controls) or T4 (0.2 mg/100 g BW, ip, once daily) for 6 days. At the end of the treatment period, the uptake of 32Pi (at 30 sec), in the presence of a Na+ gradient (Na+o greater than Na+i) by BBMV prepared from renal cortex of T4-treated Hyp mice, was significantly increased (+25%) compared to that in vehicle-treated Hyp controls. The uptake of L-[3H]proline (at 10 sec) by the same BBMV preparation was unchanged. T4-treated Hyp mice displayed a significant decrease in urinary Pi excretion (-31%), and a restoration of plasma Pi levels (+52%) to the normal range. Plasma calcium, creatinine, and magnesium as well as urinary excretion of cAMP did not change with T4 treatment. Another group of Hyp mice was given T3 (0.2 mg/100 g BW, ip) at three 12-h intervals. After 36 h, the rate of 32Pi uptake by renal BBM was significantly increased (+40%). Plasma Pi also increased (+26%). Our findings indicate that parenteral administration of thyroid hormones specifically increased (compared to transport of L-proline) the capacity of Na+ gradient-dependent renal BBM Pi uptake in Hyp mice, decreased renal Pi excretion, and increased plasma Pi. These results suggest that treatment with T4 or T3 can enhance the defective proximal tubular reabsorption of Pi and improve renal Pi retention in murine X-linked hypophosphatemia.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Kiebzak GM,Dousa TPdoi
10.1210/endo-117-2-613subject
Has Abstractpub_date
1985-08-01 00:00:00pages
613-9issue
2eissn
0013-7227issn
1945-7170journal_volume
117pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The primate brain was thought to contain only the GnRH known as mammalian GnRH (mGnRH). This study investigates whether a second form of GnRH exists within the primate brain. We found that brain extracts from adult stumptail and rhesus monkeys contained two forms of GnRH that were similar to mGnRH and chicken GnRH-II ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5592
更新日期:1997-12-01 00:00:00
abstract::We previously reported the existence of a descending multisynaptic, pituitary-independent, neural pathway between the hypothalamus and the testes in the male rat. Stimulation of this pathway by the intracerebroventricular (icv) injection of IL-1beta or corticotropin-releasing factor blunts the testosterone (T) respons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1441
更新日期:2004-04-01 00:00:00
abstract::The Djungarian hamster displays photoperiodic variations in gonadal size synchronized to the seasons by the nightly secretion of the pineal hormone melatonin. In short photoperiod (SP), the gonads regress in size, and circulating sex steroids levels decline. Thus, the brain is subject to seasonal variations of both me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1039
更新日期:2011-10-01 00:00:00
abstract::During the preovulatory and estradiol-progesterone-induced GnRH-LH surge, a subpopulation of GnRH neurons transiently expresses the transcription factor c-fos, which is a useful marker of cell activation. To further characterize this subpopulation of GnRH neurons, multiple immunohistochemical procedures were applied t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7299
更新日期:2000-02-01 00:00:00
abstract::Light and electron microscopic triple immunostaining was performed on coronal vibratome sections prepared from the hypothalamus of ovariectomized (OVX) and OVX plus estrogen-treated African green monkeys (Cercopithecus aethiops). Immunoreactivity for progesterone receptors (PRs) and neuropeptide-Y (NPY) was visualized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8100520
更新日期:1993-07-01 00:00:00
abstract::The induction of long-term responses in the uterus following estrogen treatment is discussed, with special reference to DNA synthesis. Immature female rats injected daily with estradiol-17beta or estriol (0.01 to 1 mug) or a combination of the two steroids for one, two or three consecutive days were sacrificed at inte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1501
更新日期:1976-12-01 00:00:00
abstract::Orally delivered salt stimulates renal salt excretion more effectively than does iv delivered salt. Although the mechanisms that underlie this "postprandial natriuresis" are poorly understood, the peptide uroguanylin (UGn) is thought to be a key mediator. However, the lack of selective assays for UGn gene products has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1725
更新日期:2008-09-01 00:00:00
abstract::Mouse β-cell-specific reporter lines have played a key role in diabetes research. Although the rat provides several advantages, its use has lagged behind the mouse due to the relative paucity of genetic models. In this report we describe the generation and characterization of transgenic rats expressing a Renilla lucif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1550
更新日期:2016-03-01 00:00:00
abstract::Female infertility is often associated with deregulation of hormonal networks, and hyperprolactinemia is one of the most common endocrine disorders of the hypothalamic-pituitary axis affecting the reproductive functions. We have shown previously that transgenic female mice overexpressing human chorionic gonadotropin β...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1393
更新日期:2012-12-01 00:00:00
abstract::There is a growing body of evidence that subtle decreases in maternal thyroid hormone during gestation can impact fetal brain development. The present study examined the impact of graded levels of thyroid hormone insufficiency on brain development in rodents. Maternal thyroid hormone insufficiency was induced by expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1276
更新日期:2007-06-01 00:00:00
abstract::Hyperandrogenism is the central feature of polycystic ovary syndrome (PCOS). Due to the intricate relationship between hyperandrogenism and insulin resistance in PCOS, 50%-70% of these patients also present with hyperinsulinemia. Metformin, an insulin sensitizer, has been used to reduce insulin resistance and improve ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1733
更新日期:2016-01-01 00:00:00
abstract::The effect of amino acid substitutions introduced to the third alpha-helix in bovine GH (bGH) was investigated. A GH analog (bGH-M8), in which three amino acids were substituted to form an idealized amphiphilic alpha-helix, possessed the same specific binding affinity as wild-type bGH to cell membranes prepared from 3...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547740
更新日期:1992-04-01 00:00:00
abstract::Insulin radioreceptor assays were developed using splenic leukocytes and peripheral erythrocytes from individual mice. Splenic leukocytes were prepared using an NH4Cl buffer which did not alter insulin binding, but gave much higher yields than density gradient methods. Mouse erythrocytes were isolated from heparinized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-2-474
更新日期:1982-02-01 00:00:00
abstract::Although prolactinomas can be effectively treated with dopamine agonists, about 20% of patients develop dopamine resistance or tumor recurrence after surgery, indicating a need for better understanding of underlying disease mechanisms. Although estrogen-induced rat prolactinomas have been widely used to investigate th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1056
更新日期:2012-08-01 00:00:00
abstract::The actions and the mechanisms of action of epidermal growth factor (EGF) in testicular steroidogenesis were investigated using a model of primary culture of purified porcine Leydig cells from immature intact animals. EGF decreased (1.7-fold) human CG (hCG)-induced dehydroepiandrosterone (DHEA) accumulation in the med...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-4-2160
更新日期:1991-04-01 00:00:00
abstract::The physicochemical and immunological properties of androgen receptors from kidney and brain of testicular-feminized (Tfm) mutant mice and wild-type mice were compared. Analysis by gel filtration and sucrose density gradients revealed that the mol wt of the mutant receptor was 66K (38A; 3.8S) which was significantly s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-771
更新日期:1989-02-01 00:00:00
abstract::To test the hypothesis that leptin secreted from adipose tissue is a mediator linking obesity and pancreatic islet hypertrophy, we examined the effects of leptin on proliferative and apoptotic responses in rat islet cells. Rat pancreatic islets were isolated and incubated with 0, 1, 5, or 75 nM leptin for 24 h under s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.11.8494
更新日期:2001-11-01 00:00:00
abstract::Hereditary vitamin D-resistant rickets (HVDRR) is an autosomal recessive disease caused by mutations in the vitamin D receptor (VDR). We studied a young Saudi Arabian girl who exhibited the typical clinical features of HVDRR, but without alopecia. Analysis of her VDR gene revealed a homozygous T to C mutation in exon ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0080
更新日期:2004-11-01 00:00:00
abstract::Prolactin (PRL) is a vertebrate hormone with diverse actions in osmoregulation, metabolism, reproduction, and in growth and development. Osmoregulation is fundamental to maintaining the functional structure of the macromolecules that conduct the business of life. In teleost fish, PRL plays a critical role in osmoregul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1969
更新日期:2016-09-01 00:00:00
abstract::We recently identified a novel hypothalamic neuropeptide stimulating GH release in bullfrogs and termed it frog GH-releasing peptide (fGRP). The fGRP precursor encodes fGRP and its related peptides (fGRP-RP-1, -RP-2, and -RP-3), and fGRP-RP-2 also stimulates GH and prolactin (PRL) release. Cell bodies and terminals co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1427
更新日期:2008-03-01 00:00:00
abstract::We have studied calmodulin (CaM)-like activity and calcium (Ca++)-regulated phosphodiesterase (PDE) activity in cells from the rat adrenal zona glomerulosa (ZG) and zona fasciculata (ZF). Boiled cell sonicates from the ZG and ZF activated CaM-deficient PDE in a dose-dependent fashion by 2.0- to 2.3-fold. The propertie...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-2-485
更新日期:1983-08-01 00:00:00
abstract::IGF1-a small, single-chain, secreted peptide in mammals-is essential for normal somatic growth and is involved in a variety of other physiological and pathophysiological processes. IGF1 expression appears to be controlled by several different signaling mechanisms in mammals, with GH playing a key role by activating an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00259
更新日期:2018-06-01 00:00:00
abstract::Serum and pituitary thyroid-stimulating hormone (TSH) was measured by radioimmunoassay in male and female Sprague-Dawley rats from 21 through 80 days of age. In males, serum TSH levels increased progressively from days 30 through 50, and were found to be lower on days 60, 70, and 80. In females, serum TSH levels were ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-6-1365
更新日期:1976-06-01 00:00:00
abstract::Mast cells are involved in atopic disorders, often exacerbated by stress, and are located perivascularly close to sympathetic and sensory nerve endings. Mast cells are activated by electrical nerve stimulation and millimolar concentrations of neuropeptides, such as substance P (SP). Moreover, acute psychological stres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5660
更新日期:1998-01-01 00:00:00
abstract::Congenital hypothyroidism with thyroid dysgenesis (TD) is a frequent human condition characterized by elevated levels of TSH in response to reduced thyroid hormone levels. Congenital hypothyroidism is a genetically heterogeneous disease. In the majority of cases studied, no causative mutations have been identified and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0882
更新日期:2005-12-01 00:00:00
abstract::A model for the study of testicular function in experimental uremia induced by subtotal nephrectomy in mature male rats is described. Glomerular filtration rate was reduced by 87%, resulting in stable uremia for up to 13 weeks with characteristic biochemical changes and reduction in weight of testes, prostate, seminal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1974
更新日期:1985-11-01 00:00:00
abstract::Rat calvariae are known to secrete a bone-derived growth factor (BDGF) that stimulates bone DNA and collagen synthesis. BDGF was purified from calvarial culture medium with dialysis, gel filtration and HPLC. Amino acid composition of BDGF was compatible with murine beta 2 microglobulin (beta 2 m), and amino terminal s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-3-1198
更新日期:1987-09-01 00:00:00
abstract::Remodeling of maternal spiral arteries during pregnancy requires a subpopulation of extravillous cytotrophoblasts (EVTs) to differentiate into endovascular EVTs. Activin A, which is abundantly expressed at the maternal-fetal interface, has been shown to promote trophoblast invasion, but its role in endovascular differ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1228
更新日期:2015-11-01 00:00:00
abstract::The testis contains many peptides originally described as originating in the central nervous system. The physiological function of these factors in the testis is generally unknown. We previously reported that the rat testis contains both a peptide with GH-releasing hormone-like immunoactivity (tGHRH-LI) and a mRNA spe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2336
更新日期:1990-11-01 00:00:00
abstract::To determine if the inhibitory effects of glucocorticoids on GnRH-stimulated secretion of LH observed in male rats in vivo are exerted directly on the pituitary, dispersed pituitary cells from adult male rats were treated with 60 or 600 ng/ml cortisol (F) or corticosterone (B) during one or two 48-h incubations. Contr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-855
更新日期:1985-09-01 00:00:00