Abstract:
:Light and electron microscopic triple immunostaining was performed on coronal vibratome sections prepared from the hypothalamus of ovariectomized (OVX) and OVX plus estrogen-treated African green monkeys (Cercopithecus aethiops). Immunoreactivity for progesterone receptors (PRs) and neuropeptide-Y (NPY) was visualized by a dark blue to black nickel diaminobenzidine reaction, while the tyrosine hydroxylase-containing perikarya were labeled with a light brown diaminobenzidine reaction. In the OVX plus estrogen-treated material, 30% of the tyrosine hydroxylase-immunoreactive neurons contained PR-immunopositive nuclei. The majority of these cells were found in the central portion of the periventricular area, and a few could be observed in the anterior hypothalamus and the arcuate and dorsomedial hypothalamic nuclei. These tyrosine hydroxylase-immunoreactive PR-containing cells were surrounded with NPY-immunoreactive axon terminals. A correlated electron microscopic analysis of the same sections revealed synaptic contacts between these NPY-immunoreactive boutons and the PR-containing tyrosine hydroxylase-immunoreactive neurons. In contrast, in the OVX animals, no PR-containing tyrosine hydroxylase-immunoreactive neurons could be detected. In these monkeys, the frequency of synaptic contacts between the NPY-immunoreactive axon terminals and tyrosine hydroxylase-immunopositive cells was similar to that in the OVX plus estrogen-treated monkeys. These observations indicate that in a population of hypothalamic dopamine cells, the presence of nuclear PRs is estrogen dependent, show that these cells are innervated by NPY axons, and suggest that these estrogen-induced PR-containing dopamine neurons are involved in mediation of the effect of NPY on hypophyseal hormone secretion, including ovarian steroid hormone-dependent LH and PRL release.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Horvath TL,Shanabrough M,Naftolin F,Leranth Cdoi
10.1210/endo.133.1.8100520subject
Has Abstractpub_date
1993-07-01 00:00:00pages
405-14issue
1eissn
0013-7227issn
1945-7170journal_volume
133pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Endocrine regulation of uterine biology is critical for embryo receptivity and human reproduction. Uterine endometrium depends on extrinsic sex steroid input and hence likely has mechanisms that enable adaptation to hormonal variation. Emerging evidence suggests that sex steroid bioavailability in the endometrium is d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1139
更新日期:2014-11-01 00:00:00
abstract::The adipokine, leptin (LEP), is a hormonal gateway, signaling energy stores to appetite-regulatory neurons, permitting reproduction when stores are sufficient. Dual-labeling for LEP receptors (LEPRs) and gonadotropins or GH revealed a 2-fold increase in LEPR during proestrus, some of which was seen in LH gonadotropes....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1132
更新日期:2014-10-01 00:00:00
abstract::Previous studies have demonstrated that estradiol-17beta and estradiol-17alpha both induce short-latency effects on spatial memory in rats, estradiol-17alpha being at least as potent as its 17beta isomer. To determine whether the mechanisms underlying these behavioral responses might include effects on hippocampal syn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0730
更新日期:2005-01-01 00:00:00
abstract::Pineal denervation by superior cervical ganglionectomy (Gx) decreased high affinity binding of estradiol (E2) to the pineal cytosol of female rats and of testosterone to the cytosol of male rats by 40 and 26% and by 75 and 80%, 5 and 14 days after sugery; hormone binding remained unchanged up to 24 h after surgery. Bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-4-849
更新日期:1976-04-01 00:00:00
abstract::Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis because a loss-of-function mutation in the SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1492
更新日期:2004-10-01 00:00:00
abstract::Extracts of rat pancreas contain significant amounts of an [3H]estradiol-binding protein. The amount of steroid-binding activity that could be measured varied considerably depending on the tonicity of the homogenizing medium. High speed supernatants of homogenates initially prepared in isotonic buffer contained about ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2221
更新日期:1985-06-01 00:00:00
abstract::Studies on hormone-receptor interactions generally assume that the formation of a hormone-receptor complex is a reversible process. This assumption has been examined directly in three experiments using liver membrane receptor preparations from pregnant rats and ovine PRL (oPRL). In Exp 1, Receptors were preincubated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-1-402
更新日期:1980-01-01 00:00:00
abstract::Stress-responsive neurons of the hypothalamic paraventricular nucleus (PVN) show functional plasticity and adapt to repeated restraint (RR) stress. To investigate whether neuronal adaptation to the homotypic stress also affects their response to a heterotypic stressor, we used in situ hybridization with intronic and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.8.6943
更新日期:1999-08-01 00:00:00
abstract::Isolated granulosa cells and theca from proestrous hamsters alone and in recombination, were cultured in order to study steroidogenic capacity of this tissue. Cells from medium size antral follicles (100-300 mum diam.) and large preovulatory follicles (500 plus mum diam.) were used. Steroids were measured by radioimmu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-3-694
更新日期:1975-03-01 00:00:00
abstract::To determine the relative role of the androgenic and/or estrogenic components of the action of dehydroepiandrosterone (DHEA) on the histomorphology and structure of the rat mammary gland, ovariectomized (OVX) female animals received DHEA administered alone or in combination with the pure antiandrogen flutamide or the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5762
更新日期:1998-02-01 00:00:00
abstract::Adrenocortical carcinoma (ACC) is an aggressive cancer with a 5-year survival rate <35%. Mortality remains high due to lack of targeted therapies. Using bioinformatic analyses, we identified maternal embryonic leucine zipper kinase (MELK) as 4.1-fold overexpressed in ACC compared with normal adrenal samples. High MELK...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00310
更新日期:2018-07-01 00:00:00
abstract::The effects of L-leucine, D-leucine, and L-isoleucine upon the secretion of glucagon and insulin were investigated using the isolated, perfused rat pancreas. All experiments were conducted in the presence of 5.6 mM D-glucose. Ten-minute perfusions of 2, 5, and 10 mM L-leucine induced the release of glucagon and insuli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1208
更新日期:1978-10-01 00:00:00
abstract::To better understand the role of peptide growth factors in sex steroid hormone-mediated growth of the female reproductive tract, the effect of estrogen on the expression of transforming growth factor-alpha (TGF alpha) in mouse uterus was investigated. Our results show that estrogen induces the expression of TGF alpha ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1396310
更新日期:1992-10-01 00:00:00
abstract::Estrogen deficiency causes bone loss by increasing the number of bone-resorbing osteoclasts. Selective p38α MAPK inhibitors prevent bone-wasting effects of estrogen withdrawal but implicated mechanisms remain to be identified. Here, we show that inactivation of the p38α-encoding gene in osteoblast lineage cells with t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1669
更新日期:2015-12-01 00:00:00
abstract::Lefty/Ebaf polypeptides, novel members of the TGF-beta superfamily, are involved in endometrial differentiation and embryo implantation. Recently, we showed that, during undisturbed estrous cycle, lefty is present in mouse uterine horn primarily in a precursor form. Here, we show that decidual differentiation of endom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0684
更新日期:2005-12-01 00:00:00
abstract::Maternal nutrient restriction at specific stages of gestation has differential effects on fetal development such that the offspring are programmed to be at increased risk of adult disease. We investigated the effect of gestational age and maternal nutrition on the maternal plasma concentration of leptin and cortisol t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0320
更新日期:2003-08-01 00:00:00
abstract::In temperate zones, animals restrict breeding to specific seasons to maximize the survival of their offspring. Birds have evolved highly sophisticated mechanisms of seasonal regulation, and their testicular mass can change 100-fold within a few weeks. Recent studies on Japanese quail revealed that seasonal gonadal dev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1741
更新日期:2015-02-01 00:00:00
abstract::The estrogen-induced and -dependent Syrian hamster renal tumor is the most intensively studied model in estrogen carcinogenesis. Yet, it remains confounding that the kidney of this species behaves as an estrogen target tissue. As both reproductive and urinary systems arise from the same germinal ridge, we propose that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8355
更新日期:2001-09-01 00:00:00
abstract::Is it well known that LHRH agonists can inhibit testicular functions by gonadal desensitization secondary to endogenous LH release and that a direct action at the gonadal level has also been demonstrated. Since an excess of anti-LH serum can be used, as an alternative to hypophysectomy, to neutralize the influence of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-2-524
更新日期:1982-02-01 00:00:00
abstract::Type 1 diabetes is caused by autoimmune destruction of β-cells. Although immunotherapy can restore self-tolerance thereby halting continued immune-mediated β-cell loss, residual β-cell mass and function is often insufficient for normoglycemia. Using a growth factor to boost β-cell mass can potentially overcome this ba...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1549
更新日期:2016-01-01 00:00:00
abstract::The follicular phase rise in circulating estradiol causes a suppression of GnRH pulse amplitude and an increase in pulse frequency before stimulation of the preovulatory GnRH surge in the ewe. Studies in which samples were collected at 10-min intervals were found unsuitable to determine whether GnRH secretion is exclu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7895670
更新日期:1995-04-01 00:00:00
abstract::Adenylate cyclase of rat renal cortex was inhibited by angiotensin II (AII). Inhibition required Na+ (100-200 mM) and GTP (10(-8)-10(-4) M) and was opposed by the receptor antagonist [1-sarcosine, 8-isoleucine]AII. The EC50 value (+/- SE)for inhibition by AII was 3.7 +/- 1.2 nM, and the maximum inhibition (+/- SE) was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-5-1687
更新日期:1982-11-01 00:00:00
abstract::In mammals, adrenal medulla chromaffin cells constitute a fundamental component of the sympathetic nervous system outflow, producing most of the circulating adrenaline. We recently found that the rhesus monkey adrenal gland expresses several genes in a 24-h rhythmic pattern, including TH (the rate-limiting enzyme in c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0610
更新日期:2007-12-01 00:00:00
abstract::The scaffolding protein, protein targeting to glycogen (PTG), orchestrates the signaling of several metabolic enzymes involved in glycogen synthesis. However, little is known concerning the regulation of PTG itself. In this study, we have cloned and characterized the mouse promoter of PTG. We identified multiple FoxA2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1513
更新日期:2006-07-01 00:00:00
abstract::Androgens play a critical role in the development and progression of prostate cancer (PCa), and androgen deprivation therapy via surgical or medical castration is front-line therapy for patients with advanced PCa. However, intratumoral testosterone levels are elevated in metastases from patients with castration-resist...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2014-1337
更新日期:2014-11-01 00:00:00
abstract::To determine the relationship between the concentrations of serum T3 and hepatic nuclear T3 receptor in a reversible nonthyroidal disease, we studied these parameters in rats with streptozotocin-induced diabetes mellitus, control rats, and rats that were weight-matched to the diabetic rats by food restriction. Results...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-4-1259
更新日期:1981-10-01 00:00:00
abstract::The activity of many endocrine cells is regulated by gamma-aminobutyric acid (GABA). The effects of GABA are mediated by GABAA and/or GABAB receptors. While GABAB receptors in the central nervous system have now been extensively characterized, little is known of the function and pharmacology of GABAB receptors on endo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8381070
更新日期:1993-02-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine produced by peripheral immune cells and also by endocrine cells in the anterior pituitary gland. MIF exerts its proinflammatory actions in the host-defense system by blocking the inhibitory effects of glucocorticoids on the release of other pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0946
更新日期:2005-01-01 00:00:00
abstract::Steroid hormone synthesis is a vital function of the adrenal cortex, serves a critical role in gonadal function, and maintains pregnancy if normally executed in the placenta. The substrate for the synthesis of all steroid hormones is cholesterol, and its conversion to the first steroid, pregnenolone, by the cholestero...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0541
更新日期:2009-02-01 00:00:00
abstract::Chronically elevated circulating glucocorticoid levels are although to enhance vulnerability to psychopathology. Here we hypothesized that such sustained glucocorticoid levels, disturbing corticosterone pulsatility, attenuate glucocorticoid receptor signaling and target gene responsiveness to an acute challenge in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1119
更新日期:2010-03-01 00:00:00