Abstract:
:Previous studies have demonstrated that estradiol-17beta and estradiol-17alpha both induce short-latency effects on spatial memory in rats, estradiol-17alpha being at least as potent as its 17beta isomer. To determine whether the mechanisms underlying these behavioral responses might include effects on hippocampal synaptic plasticity, CA1 pyramidal spine synapse density (PSSD) was measured in ovariectomized rats within the first few hours after s.c. estrogen injection. PSSD increased markedly (by 24%) 4.5 h after the administration of 45 microg/kg estradiol-17beta. The PSSD response was significantly greater (44% above control) 30 min after estradiol-17beta injection and was markedly dose dependent; a 3-fold lower estradiol-17beta dose (15 microg/kg) did not significantly affect CA1 PSSD at either 30 min or 4.5 h. Estradiol-17alpha was a more potent inducer of PSSD than estradiol-17beta. Dose-response analysis determined an ED50 for the effect of estradiol-17alpha on PSSD of 8.92 +/- 1.99 microg/kg, with a maximal response at 15 microg/kg. These results demonstrate that high doses of estradiol induce rapid changes in CA1 PSSD. CA1 spine synapse formation appears to be more sensitive to estradiol-17alpha than to estradiol-17beta, paralleling previous data on the effects of these two steroids on spatial memory. Rapid remodeling of hippocampal synaptic connections may thus contribute to the enhancement of spatial mnemonic processing observed within the first few hours after estrogen treatment. The potency of estradiol-17alpha suggests that hormone replacement therapy using this steroid might be useful clinically in ameliorating the impact of low endogenous estrogen production on the development and progression of neurodegenerative disorders involving the hippocampus.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
MacLusky NJ,Luine VN,Hajszan T,Leranth Cdoi
10.1210/en.2004-0730subject
Has Abstractpub_date
2005-01-01 00:00:00pages
287-93issue
1eissn
0013-7227issn
1945-7170pii
en.2004-0730journal_volume
146pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Both serotonin (5-HT) and opiates exert a stimulatory effect on PRL secretion. Some evidence suggests that the action of opiates may be elicited through serotonergic neurons. We tested this hypothesis in the present study by evaluating the effect of perturbation of the serotonergic system on PRL secretion induced by f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-4-1863
更新日期:1989-10-01 00:00:00
abstract::PTH-related protein (PTHrP) has been shown to be a major factor responsible for hypercalcemia of malignancy. PTHrP acts via the PTH/PTHrP receptor, and therefore, PTH antagonists might be expected to reverse the hypercalcemia in malignancy. In the present studies, the PTH antagonists [Tyr34]bovine (b) PTH-(7-34)NH2, [...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156920
更新日期:1994-05-01 00:00:00
abstract::Methylamine and monensin are two agents known to interfere with the recycling of membrane receptors. In the present investigation, we studied their effects on the binding, intracellular distribution, and action of insulin in isolated pancreatic acini prepared from diabetic mice. These drugs had several similar effects...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-205
更新日期:1984-07-01 00:00:00
abstract::Mitochondrial reactive oxygen species have been implicated in both diabetic complications and the progression of the underlying diabetic state. However, it is not clear whether mitochondria of diabetic origin are intrinsically altered to generate excess reactive oxygen species independent of the surrounding diabetic m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0404
更新日期:2009-01-01 00:00:00
abstract::The rapid and temporary suppression of reproductive behavior is often assumed to be an important feature of the adaptive acute stress response. However, how this suppression operates at the mechanistic level is poorly understood. The enzyme aromatase converts testosterone to estradiol in the brain to activate reproduc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1341
更新日期:2011-11-01 00:00:00
abstract::We previously described significant differences in maximal testosterone production by Leydig cells from the following strains of inbred mice: C57BL/10J, C57BL/6J, DBA/2J, and C3H/HeJ. To evaluate whether these differences in maximal testosterone production related to the activities of the steroidogenic enzymes of the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1500
更新日期:1984-10-01 00:00:00
abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588270
更新日期:1995-12-01 00:00:00
abstract::To investigate trafficking in neuroendocrine cells, green fluorescent protein (GFP) tags were fused to various portions of the preproneuropeptide Y (NPY) precursor. Two neuroendocrine cell lines, AtT-20 corticotrope tumor cells and PC-12 pheochromocytoma cells, along with primary anterior pituitary cells, were examine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.7929
更新日期:2001-02-01 00:00:00
abstract::The influences of mating on serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels were evaluated in 4-day cyclic proestrous rats of the Wistar strain. Blood samples were collected via guillotine from mated and unmated controls at 1900-2000 h or 2100--2200 h. Serum LH levels were determined by tw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-394
更新日期:1977-02-01 00:00:00
abstract::To elucidate the roles of the hypothalamic peptides, GH-releasing hormone (GRH) and somatostatin (SRIH), potentially responsible for altered GH dynamics in diabetes, we studied the time courses of their changes in level associated with altered GH secretion in streptozotocin (STZ)-induced diabetic mice. Diabetic mice w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664670
更新日期:1995-10-01 00:00:00
abstract::Functional disruption of either MC3R or MC4R results in obesity, implicating both in the control of energy homeostasis. The ligands for these receptors are derived from the prohormone proopiomelancortin (POMC), which is posttranslationally processed to produce a set of melanocortin peptides with a range of activities ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0866
更新日期:2006-12-01 00:00:00
abstract::It was shown previously that epidermal growth factor (EGF) plays a role in the testosterone-dependent fetal Wolffian duct differentiation. In this communication, the role of EGF was further investigated by determining whether EGF receptor plays a role in mediating the effect of EGF during Wolffian duct (male duct) dif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404620
更新日期:1993-10-01 00:00:00
abstract::The present studies examine the initial events in the action of PTH, namely receptor binding and adenylate cyclase activation in the postobstructed canine kidney. Both kidneys were removed from five mongrel dogs 2 h after relief of unilateral ureteral obstruction (UUO) of 42 h duration. Basolateral membranes (BLM) wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1060
更新日期:1985-03-01 00:00:00
abstract::TRH is a potent stimulator of pituitary TSH release, but its function in the physiological regulation of thyroid activity is still controversial. The purpose of the present study was to investigate TRH and catecholamine secretion into hypophysial portal blood of hypothyroid and hyperthyroid rats, and in rats bearing p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-523
更新日期:1988-07-01 00:00:00
abstract::Chronically elevated fatty acids contribute to insulin resistance through poorly defined mechanisms. Endoplasmic reticulum (ER) stress and the subsequent unfolded protein response (UPR) have been implicated in lipid-induced insulin resistance. However, the UPR is also a fundamental mechanism required for cell adaptati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1881
更新日期:2012-05-01 00:00:00
abstract::In mammals, lactation is associated with a period of infertility characterized by the loss of pulsatile secretion of GnRH and cessation of ovulatory cycles. Despite the importance of lactational infertility in determining overall fecundity of a species, the mechanisms by which the suckling stimulus suppresses GnRH sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1621
更新日期:2014-01-01 00:00:00
abstract::The central characteristic of uterine fibroids is excessive deposition of extracellular matrix (ECM), which contributes to fibroid growth and bulk-type symptoms. Despite this, very little is known about patterns of ECM protein expression in fibroids and whether these are influenced by the most common genetic anomalies...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03018
更新日期:2018-02-01 00:00:00
abstract::There is considerable evidence that CRH can suppress food intake. As hypothalamic CRH, a main site of CRH expression, is also negatively regulated by glucocorticoids, it is unclear whether anorexia and weight loss in adrenal insufficiency are attributable to elevated CRH or to decreased glucocorticoid levels. To disti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6416
更新日期:1999-01-01 00:00:00
abstract::During early pseudopregnancy, DNA synthesis and mitosis in the uterine endometrial stroma precede the development of uterine sensitivity to deciduogenic stimuli. Progesterone redirects the effects of estradiol on endometrial DNA synthesis from the luminal epithelium to the stroma. To determine the time and hormonal co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1302
更新日期:1984-10-01 00:00:00
abstract::cAMP-dependent protein kinase activity was present in a soluble TSH receptor fraction. The Km of this enzyme was 2.2 X 10(-6) M for casein substrate in the absence or presence of 10(-5) M cAMP. A [3H]cAMP-binding protein was also found in this fraction. The Ka for [3H]cAMP-binding was 0.11 X 10(6) M-1, with a total bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-6-1739
更新日期:1979-06-01 00:00:00
abstract::Transfer of corticosterone from serum to milk was studied in lactating rats after ether and pentobarbital anesthesia. Two minutes after the onset of anesthesia stress, total serum and milk corticosterone concentrations were not significantly different. By 30 min, corticosterone concentrations in serum increased to a p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1364
更新日期:1984-10-01 00:00:00
abstract::We recently identified a 32 K mol wt insulin-like growth factor (IGF)-binding protein (BP) which is markedly increased in the serum of streptozotocin-diabetic rats and recognized by antiserum against the human amniotic fluid IGFBP (hIGFBP-1). In the present study we sought to confirm that this protein represents the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-789
更新日期:1990-08-01 00:00:00
abstract::Dispersed Leydig cells were prepared from pig testes and purified in a discontinuous Percoll gradient. About 95% of these cells stained for 3 beta-hydroxysteroid dehydrogenase. The cells were cultured in a chemically defined medium. Testosterone production was low (2 +/- 0.4 ng/10(6) cells/day) under basal conditions,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-6-1952
更新日期:1983-06-01 00:00:00
abstract::T3 stimulates in vitro GH secretion, whereas somatomedins have been shown to suppress GH. We, therefore, tested the effects of a recombinant human insulin-like growth factor analog (IGF-I; Thr 59) on T3-induced GH secretion. Pituitary monolayer cultures were derived from 4- to 7-week surgically thyroidectomized male r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1483
更新日期:1986-04-01 00:00:00
abstract::Human decidual cells synthesize and release decidual PRL (dPRL) immunologically and biochemically identical to human pituitary PRL. However, stimulators and inhibitors of PRL secretion such as TRH, bromocriptine or dopamine have no effect on dPRL release. The evidence for the involvement of Ca2+ in dPRL release is bas...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597150
更新日期:1992-06-01 00:00:00
abstract::A surge of GnRH release signals the LH surge that triggers ovulation. The GnRH surge is dependent on a switch in estradiol feedback from negative to positive and, in rodents, a daily neural signal, likely from the suprachiasmatic nuclei. Vasoactive intestinal polypeptide (VIP) may be involved in suprachiasmatic nuclei...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1098
更新日期:2008-06-01 00:00:00
abstract::The influence of LY117018 and tamoxifen on established uterotropic influence of estradiol was examined in immature ovariectomized rats. When LY117018 was introduced on the fourth day of estradiol treatment, it regressed the uterotropic effect of estradiol. However, tamoxifen did not attenuate estradiol activity under ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-3-987
更新日期:1981-09-01 00:00:00
abstract::Endocrine-disrupting chemicals (EDCs) that interfere with the steroid axis can affect amelogenesis, leading to enamel hypomineralization similar to that of molar incisor hypomineralization, a recently described enamel disease. We investigated the sex steroid receptors that may mediate the effects of EDCs during rat am...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1342
更新日期:2016-11-01 00:00:00
abstract::The importance of locally produced insulin-like growth factor-I (IGF-I) in connective tissues has recently been recognized. It has been postulated that the action of anabolic hormones on bone may be mediated through local IGF-I release. However, whether IGF-I can also be modulated by other locally acting cytokines has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-4-1635
更新日期:1990-10-01 00:00:00
abstract::The effects of electrical and chemical stimulations of the hypothalamus on the plasma levels of glucagon, insulin, and glucose were studied in undisturbed and free-moving rabbits. Electrical stimulation of the ventromedial hypothalamic nucleus (VMH) caused a marked increase in the circulating level of glucagon coincid...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-605
更新日期:1981-02-01 00:00:00