Steroidogenesis of cultured purified pig Leydig cells: effects of lipoproteins and human chorionic gonadotropin.

abstract：:The classical model for the mechanism of action of steroids holds that unbound receptors for steroids reside exclusively in the cytoplasmic compartment and that they undergo translocation to the nucleus when bound to steroids in a process which is temperature sensitive. We have in the past proposed that unbound recept...

journal_title：Endocrinology

authors： Sheridan PJ,Buchanan JM,Anselmo VC,Martin PM

更新日期：1981-04-01 00:00:00

abstract：:The effects of continuous high GH levels on GH signal transduction through the GH receptor (GHR)/Janus kinase 2 (JAK2)/signal transducer and activator of transcription 5 (STAT5) pathway as well as the desensitization of this pathway by suppressors of cytokine signaling (SOCS) were studied in transgenic mice overexpres...

journal_title：Endocrinology

authors： González L,Miquet JG,Sotelo AI,Bartke A,Turyn D

更新日期：2002-02-01 00:00:00

abstract：:A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and...

journal_title：Endocrinology

authors： Husain A,Smeby RR,Wilk D,Dzau VJ,Bumpus FM

更新日期：1984-06-01 00:00:00

abstract：:Adrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal...

journal_title：Endocrinology

authors： Wong LY,Cheung BM,Li YY,Tang F

更新日期：2005-03-01 00:00:00

abstract：:The regulation of GRF secretion was studied using a fetal rat hypothalamic cell culture system. The cells were subjected to short term release experiments on days 10-18 after plating, and GRF secretion was assessed by RIA. The identity of GRF immunoreactivity in the incubation medium was confirmed by reverse phase liq...

journal_title：Endocrinology

authors： Baes M,Vale WW

更新日期：1989-01-01 00:00:00

abstract：:Pancreastatin, a novel 49-amino acid peptide isolated from porcine pancreas, shows over 70% sequence homology to the central part of bovine and human chromogranin-A. Using an N-terminal and C-terminal synthetic peptide, we developed two sensitive and specific RIAs for the detection of pancreastatin-like immunoreactivi...

journal_title：Endocrinology

authors： Schmidt WE,Siegel EG,Lamberts R,Gallwitz B,Creutzfeldt W

更新日期：1988-09-01 00:00:00

abstract：:The heart has epicardial adipose tissue that produces adipokines and mesenchymal stem cells. Systemic adipose tissue is involved in the pathophysiology of obesity-related heart diseases. However, the method for analyzing the direct interaction between adipose tissue and cardiomyocytes has not been established. Here we...

journal_title：Endocrinology

authors： Anan M,Uchihashi K,Aoki S,Matsunobu A,Ootani A,Node K,Toda S

更新日期：2011-04-01 00:00:00

abstract：:The effects of insulin treatment and dietary glucose on the responsiveness of adipose tissue to insulin and GH after hypophysectomy were studied. Male rats, 130-150 g, were hypophysectomized. Glucose metabolism was measured by determining the production of CO2 from [14C]glucose and the incorporation of glucose into li...

journal_title：Endocrinology

authors： Gause I,Isaksson O,Lindahl A,Edén S

更新日期：1985-03-01 00:00:00

abstract：:In the present study we have examined the effects of FSH, forskolin, and (Bu)2cAMP on messenger RNA (mRNA) levels for all known subunits of cAMP-dependent protein kinase in rat Sertoli cells, using newly developed complementary DNA (cDNA) probes. mRNAs for the three regulatory subunits [RI alpha, RII51, (RII beta), an...

journal_title：Endocrinology

authors： Oyen O,Sandberg M,Eskild W,Levy FO,Knutsen G,Beebe S,Hansson V,Jahnsen T

更新日期：1988-06-01 00:00:00

abstract：:The estrogenic and antiestrogenic actions of the geometric isomers of tamoxifen and 4-hydroxytamoxifen were determined in a PRL synthesis assay using primary cultures of dispersed immature rat pituitary gland cells. 4-Hydroxytamoxifen was 100 times more potent as an antiestrogen than tamoxifen. The cis isomer of tamox...

journal_title：Endocrinology

authors： Jordan VC,Koch R,Langan S,McCague R

更新日期：1988-04-01 00:00:00

abstract：:In the developing follicle, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are the primary stimulators of steroidogenesis in granulosa and theca cells. The steroidogenic actions of both these gonadotropins are largely if not exclusively mediated through cAMP. Previous studies have shown that EGF and T...

journal_title：Endocrinology

authors： Gangrade BK,Davis JS,May JV

更新日期：1991-11-01 00:00:00

abstract：:A model for the study of testicular function in experimental uremia induced by subtotal nephrectomy in mature male rats is described. Glomerular filtration rate was reduced by 87%, resulting in stable uremia for up to 13 weeks with characteristic biochemical changes and reduction in weight of testes, prostate, seminal...

journal_title：Endocrinology

authors： Handelsman DJ,Spaliviero JA,Turtle JR

更新日期：1985-11-01 00:00:00

abstract：:To determine whether the insulinotropic effects of dopamine were mediated through the adrenergic system, dogs were infused with dopamine before or after various combinations of adrenergic and cholinergic blockade. Plasma glucose levels did not change significantly throughout the individual experiments, although cardio...

journal_title：Endocrinology

authors： Cyrus J,Stagner J,Samols E

更新日期：1982-07-01 00:00:00

abstract：:We recently reported that chronic 17β-estradiol (E2) treatment in mice decreases platelet responsiveness, prolongs the tail-bleeding time and protects against acute thromboembolism via the hematopoietic estrogen receptor alpha (ERα), and independently of ERβ. Here, we have explored the respective roles of membrane vs ...

journal_title：Endocrinology

authors： Valéra MC,Fontaine C,Lenfant F,Cabou C,Guillaume M,Smirnova N,Kim SH,Chambon P,Katzenellenbogen JA,Katzenellenbogen BS,Payrastre B,Arnal JF

更新日期：2015-11-01 00:00:00

abstract：:This study aimed to delineate the hypothalamic and/or pituitary actions of testosterone and its primary metabolites 5 alpha-dihydrotestosterone and estradiol (E) in adult castrated rams (wethers) during the breeding season. In Exp 1, wethers were treated for a week with twice daily injections (im) of peanut oil, 8, 16...

journal_title：Endocrinology

authors： Tilbrook AJ,de Kretser DM,Cummins JT,Clarke IJ

更新日期：1991-12-01 00:00:00

abstract：:We have investigated the effects of veratridine, a Na+ channel activator, and ouabain, an inhibitor of Na+-K+-ATPase, on short term (1-h) PRL release from primary cultures of rat anterior pituitary cells and from the rat anterior pituitary cell line GH4C1 in culture. Both compounds should increase intracellular Na+. V...

journal_title：Endocrinology

authors： Scammell JG,Dannies PS

更新日期：1983-10-01 00:00:00

abstract：:Activation of beta2-adrenergic receptors inhibits osteoblastic bone formation and enhances osteoclastic bone resorption. Whether beta-blockers inhibit ovariectomy-induced bone loss and decrease fracture risk remains controversial. To further explore the role of beta-adrenergic signaling in skeletal acquisition and res...

journal_title：Endocrinology

authors： Bouxsein ML,Devlin MJ,Glatt V,Dhillon H,Pierroz DD,Ferrari SL

更新日期：2009-01-01 00:00:00

abstract：:Peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1), a cold-induced protein expressed in brown adipose tissue (BAT), plays a role in adaptive thermogenesis by up-regulating uncoupling proteins (UCP). Here, we explore its relationship to the thermogenic actions of leptin, which also up-regulates UCPs...

journal_title：Endocrinology

authors： Kakuma T,Wang ZW,Pan W,Unger RH,Zhou YT

更新日期：2000-12-01 00:00:00

abstract：:Physiological roles for PTH-related peptide (PTHrP) appear varied, but remain to be clarified. The peptide is present in large amounts in milk, and PTHrP mRNA has been shown to be present in high amounts in lactating mammary gland. Because PTHrP can cause smooth muscle relaxation, we hypothesized that the peptide migh...

journal_title：Endocrinology

authors： Seitz PK,Cooper KM,Ives KL,Ishizuka J,Townsend CM Jr,Rajaraman S,Cooper CW

更新日期：1993-09-01 00:00:00

abstract：:Immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. The bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horse...

journal_title：Endocrinology

authors： Bubenik GA,Brown GM,Grota LJ

更新日期：1975-01-01 00:00:00

abstract：:Activin A is a member of the transforming growth factor-beta family of growth factors and a potent regulator of cellular activity. A number of binding proteins for activin A have been identified, including alpha 2-macroglobulin (alpha 2M). Alpha 2M has several conformational states that are known to have different gro...

journal_title：Endocrinology

authors： Niemuller CA,Randall KJ,Webb DJ,Gonias SL,LaMarre J

更新日期：1995-12-01 00:00:00

abstract：:Using a sensitive method for the simultaneous measurement of TSH subunit mRNAs, we have investigated their hormonal regulation in the pituitary glands of normal and hypothyroid rats. Oligodeoxyribonucleotides (probes) complementary to coding regions of rat alpha- and TSH beta-subunit mRNAs were synthesized. These prob...

journal_title：Endocrinology

authors： Carr FE,Ridgway EC,Chin WW

更新日期：1985-09-01 00:00:00

abstract：:In the present studies we examined the regulation of insulin-like growth factor I (IGF-I) expression in porcine granulosa cells in vitro. Using Northern analysis and ribonuclease protection assays with exon-specific probes, we identified the IGF-I messenger RNA (mRNA) transcripts present in these cells under basal and...

journal_title：Endocrinology

authors： Samaras SE,Canning SF,Barber JA,Simmen FA,Hammond JM

更新日期：1996-11-01 00:00:00

abstract：:A functioning rat thyroid cell line (FRTL-5) responds acutely to the addition of TSH, norepinephrine, and the Ca2+ ionophore A23187 by a depression in iodide (I-) uptake levels. The decrease in I- content measured at the steady state depends on the presence of external Ca2+ and can be accounted for by an effect on sti...

journal_title：Endocrinology

authors： Weiss SJ,Philp NJ,Grollman EF

更新日期：1984-04-01 00:00:00

abstract：:The effect of active and passive immunization with luteinizing hormone-releasing hormone (LHRH) on serum LH and follicle-stimulating hormone (FSH) levels and the ultrastructure of the pituitary gonadotrophs was investigated in castrated male rats. Two weeks after castration, the animals were immunized with Glu1-LHRH c...

journal_title：Endocrinology

authors： Arimura A,Shino M,de la Cruz KG,Rennels EG,Schally AV

更新日期：1976-07-01 00:00:00

abstract：:The study of the biochemical mechanisms regulating the differentiation of the ovarian androgen-producing cells has been difficult because of the lack of a method for isolating the theca-interstitial cells (TIC) from the granulosa and other cell types. We report here a simple, rapid and reproducible method for obtainin...

journal_title：Endocrinology

authors： Magoffin DA,Erickson GF

更新日期：1988-05-01 00:00:00

abstract：:The expression of melatonin receptors (MR) of the Mel1a subtype in basolateral membrane of guinea pig kidney proximal tubule suggests that melatonin plays a role in regulating epithelial functions. To investigate the cellular basis of melatonin action on epithelia, we sought to establish an appropriate in vitro cultur...

journal_title：Endocrinology

authors： Chan CW,Song Y,Ailenberg M,Wheeler M,Pang SF,Brown GM,Silverman M

更新日期：1997-11-01 00:00:00

abstract：:The biosynthesis of estrogens is catalyzed by aromatase P450 (P450arom), the product of the CYP19 gene. The tissue-specific expression of the CYP19 gene is regulated by means of tissue-specific promoters through the use of alternative splicing mechanisms. Thus, transcripts containing various 5'-untranslated termini ar...

journal_title：Endocrinology

authors： Sun T,Zhao Y,Mangelsdorf DJ,Simpson ER

更新日期：1998-04-01 00:00:00

abstract：:Progesterone, which is normally produced in the endoplasmic reticulum, was found to be rapidly degraded in the cytosolic fraction of the guinea pig adrenal cortex in vitro. Assuming this finding reflects what happens in vivo raises a question as to the source of progesterone for interacting with a nuclear progesterone...

journal_title：Endocrinology

authors： Demura T,Driscoll WJ,Strott CA

更新日期：1990-09-01 00:00:00

abstract：:The current study was designed to investigate the ability of prostaglandin F2 alpha (PGF2 alpha) to activate a second messenger system (phosphatidylinositol pathway) in corpora lutea (CL) of rhesus monkeys. Activation of this pathway was assessed by monitoring the hydrolysis of phosphatidylinositol to inositol phospha...

journal_title：Endocrinology

authors： Houmard BS,Guan Z,Stokes BT,Ottobre JS

更新日期：1992-08-01 00:00:00