Abstract:
:Activation of beta2-adrenergic receptors inhibits osteoblastic bone formation and enhances osteoclastic bone resorption. Whether beta-blockers inhibit ovariectomy-induced bone loss and decrease fracture risk remains controversial. To further explore the role of beta-adrenergic signaling in skeletal acquisition and response to estrogen deficiency, we evaluated mice lacking the three known beta-adrenergic receptors (beta-less). Body weight, percent fat, and bone mineral density were significantly higher in male beta-less than wild-type (WT) mice, more so with increasing age. Consistent with their greater fat mass, serum leptin was significantly higher in beta-less than WT mice. Mid-femoral cross-sectional area and cortical thickness were significantly higher in adult beta-less than WT mice, as were femoral biomechanical properties (+28 to +49%, P < 0.01). Young male beta-less had higher vertebral (1.3-fold) and distal femoral (3.5-fold) trabecular bone volume than WT (P < 0.001 for both) and lower osteoclast surface. With aging, these differences lessened, with histological evidence of increased osteoclast surface and decreased bone formation rate at the distal femur in beta-less vs. WT mice. Serum tartrate-resistance alkaline phosphatase-5B was elevated in beta-less compared with WT mice from 8-16 wk of age (P < 0.01). Ovariectomy inhibited bone mass gain and decreased trabecular bone volume/total volume similarly in beta-less and WT mice. Altogether, these data indicate that absence of beta-adrenergic signaling results in obesity and increased cortical bone mass in males but does not prevent deleterious effects of estrogen deficiency on trabecular bone microarchitecture. Our findings also suggest direct positive effects of weight and/or leptin on bone turnover and cortical bone structure, independent of adrenergic signaling.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Bouxsein ML,Devlin MJ,Glatt V,Dhillon H,Pierroz DD,Ferrari SLdoi
10.1210/en.2008-0843subject
Has Abstractpub_date
2009-01-01 00:00:00pages
144-52issue
1eissn
0013-7227issn
1945-7170pii
en.2008-0843journal_volume
150pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::A thyroid transcription factor-1 (TTF-1)-binding element in the rat TSH receptor (TSHR) promoter, between -189 and -175 basepairs (bp), is important for both thyroid-specific expression and thyroid-specific TSH/cAMP autoregulation of the TSHR. The identification of an up-stream TTF-1-binding site and its relationship ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828540
更新日期:1995-01-01 00:00:00
abstract::Bovine granulosa cells seeded in the presence of serum on extracellular matrix-coated dishes proliferate actively when exposed to serum-free medium supplemented with insulin (2 microgram/ml), fibroblast growth factor (FGF, 100 ng/ml), and high density lipoprotein (HDL, 30 microgram protein/ml). The final density of th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-409
更新日期:1981-08-01 00:00:00
abstract::Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. H...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1016
更新日期:2014-09-01 00:00:00
abstract::Rats were made unilaterally cryptorchid by cutting the gubernaculum testis at birth. At 100 days age, the rats were injected with 600 IU/kg hCG. A biphasic testosterone response was seen in the scrotal (Scr) testis in response to hCG, with maxima at 1 h and 3 days after injection. The acute peak of testosterone was of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-550
更新日期:1984-08-01 00:00:00
abstract::Organ weight was compared in adult mice with deletion of one (IRS-1-/+) or both (IRS-1-/-) copies of the insulin receptor substrate-1 (IRS-1) gene and IRS-1+/+ littermates. IRS-1-/+ mice showed modest reductions in weight of most organs in proportion to a decrease in body weight. IRS-1-/- mice showed major reductions ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7219
更新日期:1999-12-01 00:00:00
abstract::The binding of [125I]iodo-low density lipoprotein ([125I]iodo-LDL) to isolated membranes was determined for the outer (glomerulosa/fasciculata) and inner (reticularis) zones of the guinea pig adrenal cortex. Binding of [125I]iodo-LDL to membranes from both zones was found to be highly specific and dependent on the pre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-2-653
更新日期:1986-02-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::Progesterone (P4) is essential for female fertility. The objective of this study was to evaluate the functional requirement of the nonclassical P4 receptor (PGR), PGR membrane component 1, in regulating female fertility. To achieve this goal, the Pgrmc1 gene was floxed by insertion of loxP sites on each side of exon 2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1081
更新日期:2016-09-01 00:00:00
abstract::Atrial natriuretic factor (ANF) is stored in atrial myocytes as a 15-17K prohormone, but circulates in plasma as a 3K, carboxy (C)-terminal fragment of the prohormone. The tissue location at which the cleavage of pro-ANF to its hormonal form occurs is unknown. In the present study, an immunological approach was taken ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-4-2054
更新日期:1988-10-01 00:00:00
abstract::We tested the hypothesis that depressed duodenal calcium absorption in the streptozotocin diabetic rat is the consequence of diabetes rather than nephrotoxicity of the diabetogenic agent causing abnormal renal vitamin D metabolism. We treated streptozotocin diabetic rats with insulin and compared their duodenal calciu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-1-67
更新日期:1977-01-01 00:00:00
abstract::The steady state concentration of dopamine (DA), norepinephrine (NE), and serotonin (5HT) was determined and turnover estimated in several brain regions of young (3-4 months) and old (21 months) male Wistar rats. An estimate of DA and NE turnover was obtained by determining their depletion rates after treatment with a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-6-1672
更新日期:1977-06-01 00:00:00
abstract::Tuberoinfundibular peptide is a recently discovered agonist for the PTH receptor-2; the latter has a wide distribution including the external zone of the median eminence of the hypothalamus, suggesting a role in neuroendocrine function. We have investigated the effects of tuberoinfundibular peptide on the hypothalamo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8308
更新日期:2001-08-01 00:00:00
abstract::We have reported that calcitonin (CT) treatment induced downregulation of the CT receptor (CTR) in mouse osteoclast-like cells (OCLs). Here, we studied the features of homologous down-regulation of the CTR in mature mouse OCLs. Treatment with salmon CT (sCT) and human CT (hCT) reduced [125I]sCT specific binding. The d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536630
更新日期:1996-01-01 00:00:00
abstract::Circulating levels of insulin-like growth factor-I (IGF-I) increase during puberty, concurrent with an increase in the levels of GH and the gonadal steroids. The relationship between the changes observed in IGF-I and testosterone (T) levels are not understood. This study was designed to determine whether T has a direc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315260
更新日期:1992-05-01 00:00:00
abstract::The effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the 5'-deiodinase (5'D) activity was studied in rat astroglial cells cultured in chemically defined medium. TPA promoted a large increase in the type II 5'D activity, which was maximal 5-10 h after addition of TPA and then d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1277
更新日期:1989-09-01 00:00:00
abstract::The p44/p42 MAPKs (ERK1/2) cascade regulates beta-cell nuclear events, which modulates cell differentiation and gene transcription, whereas its implication in processes occurring in the cytoplasm, such as activation of the exocytotic machinery, is still unclear. Using the MIN6 beta-cell line and isolated rat islets of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0841
更新日期:2005-02-01 00:00:00
abstract::A side population (SP) has been identified in a number of tissues, where it typically represents a small population enriched in stem/progenitor cells. In this study we show that the adult mouse anterior pituitary (AP) also contains a characteristic SP displaying verapamil-sensitive Hoechst dye efflux capacity. A major...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0185
更新日期:2005-09-01 00:00:00
abstract::To determine the presence of a PRL-binding protein (PRL-BP) in rat serum, female rats were ovariectomized and administered sc estradiol benzoate capsules. Serum was incubated with [125I]rat PRL (rPRL) for 1 h at 37 C with or without different doses of rPRL, ovine PRL (oPRL), or rGH. Separation of the [125I]rPRL-PRL-BP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8504760
更新日期:1993-06-01 00:00:00
abstract::Glucagon-like peptide 1 (7-36)amide (GLP-1) is an insulinotropic intestinal peptide hormone with a potential role as antidiabetogenic therapeutic agent. It mediates a potentiation of glucose-induced insulin secretion, by activation of adenylate cyclase and subsequent elevation of cytosolic free calcium, [Ca2+]cyt. We ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6947
更新日期:1999-09-01 00:00:00
abstract::Recent studies suggest that hyperinsulinemia and insulin resistance are linked to Alzheimer's disease (AD). In this study, we used Tg2576 transgenic (Tg) mice, a widely used transgenic mouse model for AD, to explore the relationship between increased amyloid beta-peptide (Abeta) and insulin resistance. When fed a high...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1196
更新日期:2010-04-01 00:00:00
abstract::Human IGF binding protein-3 (hIGFBP-3) overexpression in mice causes hyperglycemia, but its effect on β-cell function is unknown. We compared wild-type mice with mice overexpressing hIGFBP-3 [phoshoglycerate kinase (PGK)BP3] and mutant (Gly⁵⁶/Gly⁸⁰/Gly⁸¹)hIGFBP-3 devoid of IGF binding affinity (PGKmBP3). Intraperitone...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1324
更新日期:2011-06-01 00:00:00
abstract::The developmental appearance and regulation of hypothalamic vasopressin (prepropressophysin) mRNA was studied using quantitated in situ hybridization techniques. Vasopressin mRNA levels in the supraoptic nuclei were reliably detected on day 16 of gestation, while mRNA in the suprachiasmatic nuclei (SCN) was detectable...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2483
更新日期:1987-06-01 00:00:00
abstract::It is known that renin is present in fetal membranes, with the highest concentration in the chorion laeve (reflected chorion). The purpose of this study was to identify and localize renin in human chorion laeve. Indirect immunofluorescent analysis, using antiserum against pure human kidney renin, revealed a single lay...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-4-1150
更新日期:1981-10-01 00:00:00
abstract::Leptin, a circulating 16-kDa protein secreted by adipocytes, decreases body weight by reducing food intake and enhancing energy utilization. Leptin receptors that share homology to the glycoprotein gp130 have been recently cloned. In addition, differentially spliced leptin receptor messenger RNAs have been identified....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5343
更新日期:1997-08-01 00:00:00
abstract::GnRH is expressed in first-trimester human placenta and increases cell invasion in extravillous cytotrophoblasts (EVTs). Invasive phenotypes have been reported to be regulated by transcription factor activator protein 1 (AP-1) and mesenchymal cadherin-11. The aim of our study was to investigate the roles of AP-1 compo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1871
更新日期:2015-06-01 00:00:00
abstract::Pituitary growth hormone (GH) and insulinlike growth factor (IGF)-1 are anabolic hormones whose physiological roles are particularly important during development. The activity of the GH/IGF-1 axis is controlled by complex neuroendocrine systems including two hypothalamic neuropeptides, GH-releasing hormone (GHRH) and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03175
更新日期:2018-04-01 00:00:00
abstract::The effects of epidermal growth factor (EGF) on glycogen metabolism and the binding of [125I]iodo-EGF to receptors in fetal rat hepatocytes have been examined. The actions of EGF have been compared with those of insulin-like growth factor I (IGF-I) and insulin. EGF (0.1-45 nM) stimulated dose-dependent increases in [1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-522
更新日期:1986-08-01 00:00:00
abstract::A 50-70% depletion in the anterior pituitary concentration of PRL occurred within 5 min of electrical stimulation of a single abdominal mammary nerve of urethane-anesthetized lactating rats previously nonsuckled for 6-8 h. The stimulus parameters were: 1-msec pulses, 10-20/sec at 5-30 V applied 5 sec on and 10 sec off...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-2-458
更新日期:1980-02-01 00:00:00
abstract::Childhood obesity increases the risk of adult obesity and diabetes, suggesting that early overnutrition permanently programs altered energy and glucose homeostasis. In the present studies, we used a mouse model to investigate whether early overnutrition increases susceptibility to obesity and insulin resistance in res...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1295
更新日期:2010-04-01 00:00:00
abstract::The effect of a potent agonistic LHRH analog D-Trp-6-LHRH on the hyperprolactinemia induced by haloperidol was tested in intact and ovariectomized female rats. The administration of D-Trp-6-LHRH at two dose levels (5 and 50 micrograms/day) for 20 days blocked the increase in serum PRL induced by haloperidol in intact ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2227
更新日期:1985-06-01 00:00:00