Analysis of gamma-aminobutyric acidB receptor function in the in vitro and in vivo regulation of alpha-melanotropin-stimulating hormone secretion from melanotrope cells of Xenopus laevis.

Abstract:

:The activity of many endocrine cells is regulated by gamma-aminobutyric acid (GABA). The effects of GABA are mediated by GABAA and/or GABAB receptors. While GABAB receptors in the central nervous system have now been extensively characterized, little is known of the function and pharmacology of GABAB receptors on endocrine cells. In the amphibian Xenopus laevis, GABA inhibits the release of alpha MSH from the endocrine melanotrope cells through both GABAA and GABAB receptors. We have investigated the following aspects of the GABAB receptor of the melanotrope cells of X. laevis: 1) the pharmacology of this receptor, using antagonists previously established to demonstrate GABAB receptors in the mammalian central nervous system; 2) the relative contribution to the regulation of hormone secretion by the GABAA and GABAB receptors on melanotrope cells in vitro; and 3) the role of the GABAB receptor with respect to the physiological function of the melanotrope cell in vivo, i.e. regulation of pigment dispersion in skin melanophores in relation to background color. Our results demonstrate that phaclofen, 2-hydroxysaclofen, and 4-aminobutylphosphonic acid dose-dependently blocked the inhibition of alpha MSH release by GABAB receptor activation, but not by GABAA receptor activation. The GABAB receptor antagonist delta-aminovaleric acid appeared to be a selective agonist on the GABAB receptor of melanotrope cells. The inhibitory secretory response to a low dose of GABA (10(-5) M) was not affected by bicuculline, but was significantly reduced by phaclofen, indicating that at a low GABA concentration, the GABAB receptor mechanism would dominate in inhibiting the melanotrope cells. Different thresholds of activation may form the basis for differential action of GABA through both GABA receptor types. The tonic inhibition of alpha MSH release in animals adapted to a white background was not affected by 4-aminobutylphosphonic acid, indicating that the GABAB receptor is not (solely) involved in the in vivo inhibition of alpha MSH release in animals on a white background.

journal_name

Endocrinology

journal_title

Endocrinology

authors

De Koning HP,Jenks BG,Roubos EW

doi

10.1210/endo.132.2.8381070

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

674-81

issue

2

eissn

0013-7227

issn

1945-7170

journal_volume

132

pub_type

杂志文章
  • Testosterone-responsive mouse kidney glycosphingolipids: developmental and inbred strain effects.

    abstract::Mouse kidney and urinary glycosphingolipids from developing C57BL/6J and adults of several other inbred strains were analyzed by high performance liquid chromatographic techniques. Glycosphingolipids from male and female C57BL/6J kidneys were similar until the fifth week of age. Galactosylceramide containing nonhydrox...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-113-1-251

    authors: McCluer RH,Deutsch CK,Gross SK

    更新日期:1983-07-01 00:00:00

  • Suckling, but not formula feeding, induces a transient hyperthyroxinemia in rat pups.

    abstract::To assess the effect of breast feeding on circulating thyroid hormone levels in the suckling neonate, we examined serum concentrations of T4, T3, and rT3 by RIA in 12- and 15-day-old rat pups fasted for 3 or 6 h before, at, and hourly for 3 or 4 h after suckling. In both age groups, serum T4 levels decreased significa...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-123-1-127

    authors: Oberkotter LV

    更新日期:1988-07-01 00:00:00

  • Milk protein synthesis by mammary glands of vitamin D-deficient mice.

    abstract::Mammary glands from second generation vitamin D-deficient mice and rats were examined for their ability to make the major milk proteins, casein and alpha-lactalbumin, both in vivo and in vitro. The glands from the rachitic animals were morphologically indistinguishable from those of age-matched controls. When placed i...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-121-3-865

    authors: Bhattacharjee M,Wientroub S,Vonderhaar BK

    更新日期:1987-09-01 00:00:00

  • Glucocorticoids and sex-dependent development of brain glucocorticoid and mineralocorticoid receptors.

    abstract::We have previously shown that repeated antenatal synthetic glucocorticoid exposure has sex-specific effects on hypothalamic-pituitary-adrenal development in the fetal and adult guinea pig. However, little is known about the mechanisms that underlie these sex-specific outcomes. In the current study we demonstrated that...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-0145

    authors: Owen D,Matthews SG

    更新日期:2003-07-01 00:00:00

  • Sex difference in the response of melanin-concentrating hormone neurons in the lateral hypothalamic area to glucose, as revealed by the expression of phosphorylated cyclic adenosine 3',5'-monophosphate response element-binding protein.

    abstract::Because there are sex differences in feeding behavior in rats, we looked for a possible sex difference in the response to glucose of melanin-concentrating hormone (MCH) neurons in the lateral hypothalamic area using phosphorylated cAMP response element-binding protein (pCREB) as a marker of neural activity. Intact mal...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2005-0078

    authors: Mogi K,Funabashi T,Mitsushima D,Hagiwara H,Kimura F

    更新日期:2005-08-01 00:00:00

  • Parathyroid hormone messenger ribonucleic acid in the rat hypothalamus.

    abstract::Polyadenylated RNA, extracted from rat hypothalami, cross-hybridized with a RNA probe complementary in sequence to rat PTH (rPTH) messenger RNA (mRNA). Amplification of complementary DNA (cDNA) by the polymerase chain reaction also demonstrated the presence of rPTH mRNA in the rat hypothalamus and parathyroid gland. r...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-127-5-2517

    authors: Fraser RA,Kronenberg HM,Pang PK,Harvey S

    更新日期:1990-11-01 00:00:00

  • Attenuation by a 5 alpha-reductase inhibitor of the activational effect of testosterone propionate on penile erections in castrated male rats.

    abstract::Daily administration of an inhibitor of 5 alpha-reductase, 17 beta-testosterone carboxylic acid, significantly attenuated the stimulatory effect of testosterone propionate on penile erections displayed by castrated male rats which were restrained in a supine position. This treatment, however, failed to affect mating b...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-109-4-1047

    authors: Bradshaw WG,Baum MJ,Awh CC

    更新日期:1981-10-01 00:00:00

  • Acute effects of estradiol on circulating luteinizing hormone and prolactin concentrations and on serotonin turnover in individual brain nuclei.

    abstract::Two experiments tested whether serotonergic neurons participate in the acute feedback effects of estradiol on LH and PRL secretion. In the first study, ovariectomized female rats received oil vehicle or estradiol benzoate and were killed 3 h later. The turnover of serotonin [5-hydroxytryptamine (5-HT)] in microdissect...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-113-6-1935

    authors: Johnson MD,Crowley WR

    更新日期:1983-12-01 00:00:00

  • Structure, specificity, and regulation of the insulin-like growth factor-binding proteins in adult rat serum.

    abstract::Insulin-like growth factor-I (IGF-I), the principal IGF in adult rat serum, occurs complexed to specific binding proteins. After fractionation of serum on Sephadex G-200 at neutral pH, 62% of the immunoreactive IGF-I is recovered in the 150K region, 38% in the 40K region, and none is present as free 7.5K IGF-I. Adult ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-125-3-1540

    authors: Yang YW,Wang JF,Orlowski CC,Nissley SP,Rechler MM

    更新日期:1989-09-01 00:00:00

  • Effect of estrogen-progestogen administration on tissue cation concentrations in the rat.

    abstract::Five studies are reported which were designed to define the effects of an estrogen and a progestogen, alone and in combination, on tissue concentrations (heart, kidney, and liver) of two trace metals (zinc, copper) and four bulk cations (sodium, potassium, calcium, magnesium). Male rats received Premarin (0.08 mg) and...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-97-5-1263

    authors: Yunice AA,Lindeman RD

    更新日期:1975-11-01 00:00:00

  • Constitutive expression of osteoclast-stimulating activity by normal clonal osteoblast-like cells: effects of parathyroid hormone and 1,25-dihydroxyvitamin D3.

    abstract::Osteoblasts are known to produce osteoclast-stimulating activity (OSA). The aim of the current study was to relate the expression of OSA to the osteoblastic phenotype and examine its regulation by calciotropic hormones. The study was performed with the normal osteoblastic cell clone CRP 10/30 and the preosteoblastic c...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.131.3.1505460

    authors: Collin P,Guenther HL,Fleisch H

    更新日期:1992-09-01 00:00:00

  • Sex Differences in the Hormonal and Metabolic Response to Dietary Protein Dilution.

    abstract::Consumption of a low-protein, high-carbohydrate diet induces a striking increase in circulating fibroblast growth factor-21 (FGF21), which is associated with improved cardiometabolic health and increased longevity. Increased lifespan during this dietary protein "dilution" has been explained by resource-mediated trade-...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2017-00331

    authors: Larson KR,Russo KA,Fang Y,Mohajerani N,Goodson ML,Ryan KK

    更新日期:2017-10-01 00:00:00

  • The kisspeptin/neurokinin B/dynorphin (KNDy) cell population of the arcuate nucleus: sex differences and effects of prenatal testosterone in sheep.

    abstract::Recent work in sheep has identified a neuronal subpopulation in the arcuate nucleus that coexpresses kisspeptin, neurokinin B, and dynorphin (referred to here as KNDy cells) and that mediate the negative feedback influence of progesterone on GnRH secretion. We hypothesized that sex differences in progesterone negative...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2009-0541

    authors: Cheng G,Coolen LM,Padmanabhan V,Goodman RL,Lehman MN

    更新日期:2010-01-01 00:00:00

  • Leptin signaling targets the thyrotropin-releasing hormone gene promoter in vivo.

    abstract::The regulation of TRH gene expression in the paraventricular nucleus of the hypothalamus (PVH) by leptin is critical for normal function of the thyroid axis in rodents and humans. The TRH neuron in the PVH expresses both leptin and melanocortin-4 receptors, suggesting that both signaling systems may regulate TRH gene ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2003-1312

    authors: Guo F,Bakal K,Minokoshi Y,Hollenberg AN

    更新日期:2004-05-01 00:00:00

  • Direct regulation of GnRH neuron excitability by arcuate nucleus POMC and NPY neuron neuropeptides in female mice.

    abstract::Hypothalamic neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons act to sense and coordinate the brain's responses to metabolic cues. One neuronal network that is very sensitive to metabolic status is that controlling fertility. In this study, we investigated the impact of neuropeptides released by NPY and POM...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2012-1470

    authors: Roa J,Herbison AE

    更新日期:2012-11-01 00:00:00

  • Development of a primary culture system for rainbow trout corpuscles of stannius and characterization of secreted teleocalcin.

    abstract::A primary culture system has been established for rainbow trout corpuscles of Stannius (CS). A RIA has also been developed to monitor and characterize the teleocalcin secreted by these cultures. The primary cultured CS cells actively synthesized and secreted teleocalcin for up to 39 days when maintained in amphibian a...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-123-2-913

    authors: Gellersen B,Wagner GF,Copp DH,Friesen HG

    更新日期:1988-08-01 00:00:00

  • Epigenetic Therapy: Novel Translational Implications for Arrest of Environmental Dioxin-Induced Disease in Females.

    abstract::Increased toxicant exposure and resultant environmentally induced diseases are a tradeoff of industrial productivity. Dioxin [2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD)], a ubiquitous byproduct, is associated with a spectrum of diseases including endometriosis, a common, chronic disease in women. TCDD activates cytoch...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2017-00860

    authors: Khan Z,Zheng Y,Jones TL,Delaney AA,Correa LF,Shenoy CC,Khazaie K,Daftary GS

    更新日期:2018-01-01 00:00:00

  • Increases in plasma insulin levels in response to electrical stimulation of the dorsal motor nucleus of the vagus nerve.

    abstract::In order to investigate the physiological counterpart of the anatomical finding showing that the dorsal motor nucleus of the vagus nerve (DMX) is a source of efferent vagal fibers innervating the pancreas, unilateral electrical stimulation using monopolar electrodes (50 microA, 30 Hz, 0.2 msec) at a glycemia of 150 mg...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-112-3-904

    authors: Ionescu E,Rohner-Jeanrenaud F,Berthoud HR,Jeanrenaud B

    更新日期:1983-03-01 00:00:00

  • Short pulses of low dose thyrotropin-releasing hormone do not affect thyroid hormone secretion from perfused dog thyroid lobes.

    abstract::Small amounts of TRH are present in the thyroid, and TRH has been shown in several studies to have a moderate direct inhibitory effect on TSH-stimulated thyroid hormone secretion. In a recent publication, however, data were presented to demonstrate that very low doses of TRH given in short pulses induce immediate incr...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-122-2-385

    authors: Iversen E,Laurberg P

    更新日期:1988-02-01 00:00:00

  • Protection from nicotinamide inhibition of interleukin-1 beta-induced RIN cell nitric oxide formation is associated with induction of MnSOD enzyme activity.

    abstract::We have studied the long-term effects of nicotinamide (NIC) on the synthesis of NO by insulin producing cells. NIC delays the formation of nitrite by interleukin (IL)-1 beta-(IL-1, 25 U/ml)-stimulated RINm5F cells, and previous exposure of cells to IL-1 for 15 h prevents this effect. The delay is associated with a lac...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.137.11.8895350

    authors: Andrade J,Conde M,Ramírez R,Monteseirín J,Conde J,Sobrino F,Bedoya FJ

    更新日期:1996-11-01 00:00:00

  • Prothrombin levels are increased in the estrogen-treated immature rat uterus.

    abstract::An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77,000, its N-terminal amino acid sequence is the same as prothrombin's for 15 residues, it contains gamma-carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-rea...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-126-1-167

    authors: Henrikson KP,Jazin EE,Greenwood JA,Dickerman HW

    更新日期:1990-01-01 00:00:00

  • The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens.

    abstract::The human estrogen receptor alpha (ERalpha) and the recently identified ERbeta share a high degree of amino acid homology; however, there are significant differences in regions of these receptors that would be expected to influence transcriptional activity. Consequently, we compared the mechanism(s) by which these rec...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.140.12.7179

    authors: Hall JM,McDonnell DP

    更新日期:1999-12-01 00:00:00

  • Regulation of renal 25-hydroxyvitamin D-1-hydroxylase activity in the mouse after uninephrectomy.

    abstract::Although renal hypertrophy occurs rapidly after uninephrectomy, restoring the majority of renal excretory function, it remains unknown whether similar compensatory mechanisms maintain 1,25-dihydroxyvitamin D production (and calcium homeostasis). To address this issue we compared plasma calcitriol levels and renal 25-h...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo-124-5-2118

    authors: Almond JR,Bales CW,Lobaugh B,Klotman PE,Drezner MK

    更新日期:1989-05-01 00:00:00

  • Estrogen induction of the granzyme B inhibitor, proteinase inhibitor 9, protects cells against apoptosis mediated by cytotoxic T lymphocytes and natural killer cells.

    abstract::Exposure to estrogens is associated with an increased risk of developing breast, cervical, and liver cancer. Estrogens strongly induce the human granzyme B inhibitor, proteinase inhibitor 9 (PI-9). Because cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells use the granzyme pathway to induce apoptosis of targ...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2005-0996

    authors: Jiang X,Orr BA,Kranz DM,Shapiro DJ

    更新日期:2006-03-01 00:00:00

  • Estrogen and raloxifene stimulate transforming growth factor-beta 3 gene expression in rat bone: a potential mechanism for estrogen- or raloxifene-mediated bone maintenance.

    abstract::Estrogen or raloxifene (LY156758) prevent estrogen deficiency-induced bone loss in animals and humans. We demonstrated in the rat that a 22% reduction in bone mineral density generated by ovariectomy was associated with a 2-fold reduction of transforming growth factor-beta 3 (TGF beta 3) messenger RNA expression in th...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.137.5.8612550

    authors: Yang NN,Bryant HU,Hardikar S,Sato M,Galvin RJ,Glasebrook AL,Termine JD

    更新日期:1996-05-01 00:00:00

  • Characterization and determination of the relative abundance of two types of insulin-like growth factor binding protein-5 proteases that are secreted by human fibroblasts.

    abstract::We previously reported that human fibroblasts secrete a protease into their conditioned medium that cleaves insulin-like growth factor binding protein-5 (IGFBP-5) into non-IGF-I binding fragments. Because the protease activity in the fibroblast medium has characteristics of both serine and metalloproteases, the activi...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.137.12.8940380

    authors: Nam TJ,Busby WH Jr,Clemmons DR

    更新日期:1996-12-01 00:00:00

  • Transcriptional cofactors exhibit differential preference toward peroxisome proliferator-activated receptors alpha and delta in uterine cells.

    abstract::We previously showed that peroxisome proliferator-activated receptor delta (PPARdelta) is crucial for embryo implantation as a receptor for cyclooxygenase-2-derived prostacyclin in mice. PPARs belong to the nuclear receptor superfamily. They form heterodimer with a retinoid X receptor, recruit transcriptional cofactor...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2004-0011

    authors: Lim HJ,Moon I,Han K

    更新日期:2004-06-01 00:00:00

  • Interleukin-18 is a novel mitogen of osteogenic and chondrogenic cells.

    abstract::IL-18 was identified due to its ability to induce interferon-gamma (IFNgamma) production by T cells. It is a pleiotropic factor that shares structural features with IL-1 and functional activities with IL-12. IL-18 has a role in T cell development, where it has been demonstrated to act cooperatively with IL-12 to regul...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2002-220936

    authors: Cornish J,Gillespie MT,Callon KE,Horwood NJ,Moseley JM,Reid IR

    更新日期:2003-04-01 00:00:00

  • Estrogen feedback needed at the paraventricular nucleus or A2 to suppress pulsatile luteinizing hormone release in fasting female rats.

    abstract::The feedback sites of estrogen within the hypothalamus and lower brain stem involved in the suppression of pulsatile LH secretion during 48-h fasting were examined in ovariectomized rats with local estradiol (E2) implants. The animals were ovariectomized and immediately implanted stereotaxically with stainless steel c...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/endo.135.3.8070380

    authors: Nagatani S,Tsukamura H,Maeda K

    更新日期:1994-09-01 00:00:00

  • Berberine acutely inhibits insulin secretion from beta-cells through 3',5'-cyclic adenosine 5'-monophosphate signaling pathway.

    abstract::Berberine, a hypoglycemic agent, has recently been shown to activate AMP-activated protein kinase (AMPK) contributing to its beneficial metabolic effects in peripheral tissues. However, whether berberine exerts a regulatory effect on beta-cells via AMPK or other signaling pathways and counteracts glucolipotoxicity rem...

    journal_title:Endocrinology

    pub_type: 杂志文章

    doi:10.1210/en.2007-1752

    authors: Zhou L,Wang X,Shao L,Yang Y,Shang W,Yuan G,Jiang B,Li F,Tang J,Jing H,Chen M

    更新日期:2008-09-01 00:00:00