Abstract:
:Rat calvariae are known to secrete a bone-derived growth factor (BDGF) that stimulates bone DNA and collagen synthesis. BDGF was purified from calvarial culture medium with dialysis, gel filtration and HPLC. Amino acid composition of BDGF was compatible with murine beta 2 microglobulin (beta 2 m), and amino terminal sequence analysis revealed identity with mature murine beta 2 m. This was confirmed by Western blot analysis using a polyclonal antibody to beta 2 m. Like BDGF, human beta 2 m stimulated bone DNA, collagen and noncollagen protein synthesis. Thus, BDGF, an autologous regulator of bone formation, is homologous to beta 2 m.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Canalis E,McCarthy T,Centrella Mdoi
10.1210/endo-121-3-1198subject
Has Abstractpub_date
1987-09-01 00:00:00pages
1198-200issue
3eissn
0013-7227issn
1945-7170journal_volume
121pub_type
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