Abstract:
:The formation of junctional complexes between adjacent epithelial principal cells leads to formation of the blood-epididymal barrier; this barrier is complete by 21 days of postnatal age. Cadherins are cell surface proteins that mediate intercellular adhesion and are involved in the formation of adherence, gap, and tight junctions between epithelial cells. In the adult rat epididymis, epithelial cadherin (E-Cad) is localized in principal cells; E-Cad mRNA concentrations are androgen dependent in this tissue. The objectives of this study were to determine the regulation of E-Cad mRNA concentrations and the pattern of immunocytochemical localization of E-Cad during epididymal development. Using Northern blot analysis, we noted that in the caput-corpus epididymidis, there was a 3-fold increase in E-Cad mRNA concentrations between 7-14 days; an additional 3-fold increase between days 35-42, when E-Cad mRNA concentrations reached their peak, was noted. A dramatic decrease in E-Cad mRNA was observed between 42-49 days of age. This effect was transitory as E-Cad mRNA concentrations returned to almost 80% of peak concentrations on day 56 and remained constant thereafter. In the cauda epididymidis, E-Cad mRNA concentrations increased by only 1.6-fold between days 7-21. E-Cad mRNA concentrations then decreased by 70% to their lowest concentrations on day 56. There was a 2-fold increase in E-Cad mRNA concentrations between postnatal ages 56-91 days. These results suggest that the developmental regulation of E-Cad mRNA concentrations is segment specific. A subsequent study on the longitudinal distribution of E-Cad mRNA levels in six epididymal segments at 21, 42, and 56 days of age revealed that the relative proportion of E-Cad mRNA along the epididymis changes as a function of age. An immunocytochemical study with the light microscope, using an anti-E-Cad antibody, demonstrated that the localization and relative concentrations of E-Cad varied as a function of age. On day 15, the immunoperoxidase staining of the entire epididymal epithelium was apical, with the weakest staining in the cauda epididymidis. By day 21, the reaction spread to cover the supranuclear region of the principal cells in all segments, while on day 39, it covered the entire cytoplasm of these cells, suggesting a high rate of synthesis or storage of the protein. At later time intervals, the intensity of staining over the principal cells appeared to increase with age.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Cyr DG,Hermo L,Robaire Bdoi
10.1210/endo.132.3.7679970subject
Has Abstractpub_date
1993-03-01 00:00:00pages
1115-24issue
3eissn
0013-7227issn
1945-7170journal_volume
132pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Fundamental complications of insulin resistance and type 2 diabetes include the development of nonalcoholic fatty liver disease and an atherogenic fasting dyslipidemic profile, primarily due to increases in hepatic very-low-density lipoprotein (VLDL) production. Recently, central glucagon-like peptide-2 receptor (GLP2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00416
更新日期:2018-09-01 00:00:00
abstract::Current in vivo and in vitro data indicate that progesterone (P) is required for epithelial proliferation in mammary glands of adult mice. P receptors (PgR) in adult mammary glands are under estrogenic regulation. By contrast in immature, pubertal 5-week-old mice, PgR are present only in low concentration and cannot b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-464
更新日期:1988-02-01 00:00:00
abstract::The hypoxic growth plate cartilage requires hypoxia-inducible factor (HIF)-mediated pathways to maintain chondrocyte survival and differentiation. HIF proteins are tightly regulated by prolyl hydroxylase domain-containing protein 2 (Phd2)-mediated proteosomal degradation. We conditionally disrupted the Phd2 gene in ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1473
更新日期:2016-01-01 00:00:00
abstract::Vascularly perfused duodenal loops from normal vitamin D-replete chicks were used to obtain insight with regards to the possible mechanism(s) by which 1,25-dihydroxy-vitamin D3 [1,25(OH)2D3] rapidly stimulates intestinal Ca2+ transport (transcaltachia). The phorbol ester, 12-o-tetradecanoyl phorbol-13 acetate (TPA) (1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-1-39
更新日期:1990-07-01 00:00:00
abstract::Male Sprague-Dawley rats were infused continuously for 13 days with vehicle or 75 pmol (31.2 ng)/day 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] by means of Alzet osmotic minipumps implanted s.c. Animals infused with 1,25-(OH)2D3 exhibited mild hypercalcemia (11.2 vs. 10.2 mg/dl in controls), a 136% increase in the serum ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2580
更新日期:1986-12-01 00:00:00
abstract::The possibility that chronic, multigenerational exposure to environmental estrogens selects for adaptive hormone-response phenotypes is a critical unanswered question. Embryos/larvae of killifish from an estrogenic-polluted environment (New Bedford Harbor, MA [NBH]) compared with those from a reference site overexpres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1052
更新日期:2016-06-01 00:00:00
abstract::In the pituitary, GABA regulates the release of several hormones via different receptors. GABA(C) receptors are heterooligomers that differ from GABA(A) receptors in that they contain p-subunits and are insensitive to bicuculline. However, molecular and functional evidence for the presence of GABA(C) receptors outside...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7476
更新日期:2000-05-01 00:00:00
abstract::The injection of chlorpropamide into Brattleboro homozygous rats (di/di) has previously been shown to result in enhanced activation of renal medullary adenylate cyclase activity and increased renal medullary content of cAMP in response to 1-desamino-8-D-arginine vasopressin (dDAVP). In contrast, in vivo chlorpropamide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2025
更新日期:1982-12-01 00:00:00
abstract::Cushing's disease (CD) is a rare condition in which hypercortisolemia is secondary to excessive ACTH release from a pituitary corticotroph adenoma. CD is associated with significant morbidity and mortality, and a safe therapy that effectively targets the pituitary tumor is still lacking. Retinoic acid (RA) and dopamin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1820
更新日期:2014-09-01 00:00:00
abstract::The follicular phase rise in circulating estradiol causes a suppression of GnRH pulse amplitude and an increase in pulse frequency before stimulation of the preovulatory GnRH surge in the ewe. Studies in which samples were collected at 10-min intervals were found unsuitable to determine whether GnRH secretion is exclu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7895670
更新日期:1995-04-01 00:00:00
abstract::Postnatal development of the ovine uterus between birth and postnatal day (PND) 56 involves budding differentiation of the endometrial glandular epithelium from the luminal epithelium (LE) followed by extensive coiling and branching morphogenesis of the tubular glands. To determine the short- and long-term effects of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0178
更新日期:2004-07-01 00:00:00
abstract::Relaxin was localized in cells of corpora lutea of pregnant rats at the ultrastructural level using a highly specific antirat relaxin serum and the unlabeled peroxidase-antiperoxidase technique. Electron-dense, membrane-bound granules (maximum diameter, 270 nm), which are present in luteal cells during the last third ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-4-1124
更新日期:1984-04-01 00:00:00
abstract::Epidermal growth factor (EGF) and TGFalpha are well known for their activities in the ovary. Both factors initiate signal transduction by binding to the common EGF receptor that has been demonstrated in the ovary across vertebrates from fish to humans. Using zebrafish as the model, we demonstrated in the present study...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8579
更新日期:2002-01-01 00:00:00
abstract::Topiramate (TPM) treatment has been shown to reduce adiposity in humans and rodents. The reduction in adiposity is related to decreased food intake and increased energy expenditure. However, the molecular mechanisms through which TPM induces weight loss are contradictory and remain to be clarified. Whether TPM treatme...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1272
更新日期:2012-09-01 00:00:00
abstract::The binding properties of the gamma-emitting 125I-labeled 11 beta-methoxy analog of 16 alpha-iodoestradiol, 11 beta-methoxy-16 alpha-iodoestradiol (MIE2), were characterized for its use in vivo as a ligand for the measurement and localization of estrogen-binding sites. In binding displacement studies, MIE2 bound to ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2074
更新日期:1989-05-01 00:00:00
abstract::Humans are routinely exposed to bisphenol A (BPA), an estrogenic chemical present in food and beverage containers, dental composites, and many products in the home and workplace. BPA binds both classical nuclear estrogen receptors and facilitates membrane-initiated estrogenic effects. Here we explore the ability of en...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0189
更新日期:2006-08-01 00:00:00
abstract::Four different GnRHs and one GnRH receptor are reported to be expressed in various mammals, whereas 13 GnRHs and numerous GnRH receptors have been identified in various nonmammalian vertebrates. The nucleotide sequencing of the human genome provided the opportunity to determine which of these peptides and receptors mi...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endo.143.3.8705
更新日期:2002-03-01 00:00:00
abstract::We have investigated the ability of fetal rat skin to heal an excisional wound in vitro. Skin from the backs of E17-E19 rats was wounded using a 1-mm diameter cutting needle and suspended in culture on a 6-pin cradle for 72 h. Neither contraction nor epithelial closure was observed within wounds created in skin from E...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5357
更新日期:1997-09-01 00:00:00
abstract::The fate of cell-bound [125I]iodo-human GH ([125I]iodo-hGH) was studied in monolayer cultures of hepatocytes from pregnant and nonpregnant rats. Both the binding of [125I]iodo-hGH and its degradation were significantly higher in cells from pregnant than from nonpregnant rats. The positive correlation between the numbe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1475
更新日期:1984-05-01 00:00:00
abstract::11beta-hydroxysteroid dehydrogenases (11betaHSDs) catalyze interconversion of 11-hydroxy-glucocorticoids with inactive 11-keto metabolites. In blood vessel walls, loss of 11betaHSD1 is thought to reduce local glucocorticoid concentrations, reducing the progression of atheroma and enhancing angiogenesis. Conversely, on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0996
更新日期:2007-01-01 00:00:00
abstract::Sertoli cells regulate differentiation and development of the testis and are essential for maintaining adult testis function. To model the effects of dysregulating Sertoli cell number during development or aging, we have used acute diphtheria toxin-mediated cell ablation to reduce Sertoli cell population size. Results...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00196
更新日期:2017-09-01 00:00:00
abstract::Protein kinase-C (PKC) has been suggested as a possible mediator of the antigonadotropic action of prostaglandin F2 alpha (PGF2 alpha) in luteal cells. To examine this possibility, we evaluated the effects of phorbol ester [12-O-tetradecanoylphorbol-13-acetate (TPA)] in relation to those of PGF2 alpha on cAMP accumula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1388
更新日期:1990-03-01 00:00:00
abstract::Products of the Stannius corpuscles (SC) of rainbow trout were tested in an established PTH bioassay involving bone resorption in embryonic mouse calvaria. Aqueous extracts from Stannius corpuscles (SC-homogenate) showed a bone-resorbing activity comparable to PTH in 24-h cultures of calvaria, indicated by a dose-depe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-2249
更新日期:1986-11-01 00:00:00
abstract::Morphological plasticity in response to estradiol is a hallmark of astrocytes in the arcuate nucleus. The functional consequences of these morphological changes have remained relatively unexplored. Here we report that in the arcuate nucleus estradiol significantly increased the protein levels of the two enzymes in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1701
更新日期:2009-07-01 00:00:00
abstract::Female Wistar-Furth rats were implanted sc with GH3 rat pituitary tumor cells. Tumors were palpable by 4 weeks, and animals were killed periodically from 5-9 weeks. Tumor-bearing rats (n = 10) were heavier than their respective controls, reaching a weight of 372 +/- 3 by 9 weeks vs. 195 +/- 5 g in controls (mean +/- S...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-915
更新日期:1986-03-01 00:00:00
abstract::Administration of a single injection of estradiol (E2) causes a maximum increase in DNA synthesis in the uterine luminal epithelium approximately 24 h later. We previously reported that animals receiving a second injection of E2 15-18 h after the first show an apparent decrease in DNA synthesis measured in this cell t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-3-969
更新日期:1984-03-01 00:00:00
abstract::Nowadays, we are bombarded with information on a large number of endocrine-disrupting chemicals. We hear and read about endocrine-disrupting chemicals on blogs, the web, news stories, television specials, advertisements, and of course scientific articles. Reports claim these ubiquitous compounds are responsible for in...
journal_title:Endocrinology
pub_type: 历史文章,杂志文章
doi:10.1210/en.2016-1445
更新日期:2016-08-01 00:00:00
abstract::Insulin receptor substrate-1 (IRS-1) has an important role as an early intermediary between the insulin and IGF receptors and downstream molecules that participate in insulin and IGF-I signal transduction. Here we employed an antisense oligonucleotide (IRS-1AS) to inhibit whole-body expression of IRS-1 in vivo and eva...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0778
更新日期:2005-03-01 00:00:00
abstract::The thyroid hormone (TH) analog eprotirome (KB2115) was developed to lower cholesterol through selective activation of the TH receptor (TR) β1 in the liver. Interestingly, eprotirome shows low uptake in nonhepatic tissues, explaining its lipid-lowering action without adverse extrahepatic thyromimetic effects. Clinical...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00433
更新日期:2017-10-01 00:00:00
abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyro...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2004-0959
更新日期:2004-12-01 00:00:00