Abstract:
:Agouti-related protein (AgRP) expressed in the arcuate nucleus is a potent orexigenic neuropeptide, which increases food intake and reduces energy expenditure resulting in increases in body weight (BW). Glucocorticoids, key hormones that regulate energy balance, have been shown in rodents to regulate the expression of AgRP. In this study, we generated AgRP-specific glucocorticoid receptor (GR)-deficient (knockout [KO]) mice. Female and male KO mice on a high-fat diet (HFD) showed decreases in BW at the age of 6 weeks compared with wild-type mice, and the differences remained significant until 16 weeks old. The degree of resistance to diet-induced obesity was more robust in female than in male mice. On a chow diet, the female KO mice showed slightly but significantly attenuated weight gain compared with wild-type mice after 11 weeks, whereas there were no significant differences in BW in males between genotypes. Visceral fat pad mass was significantly decreased in female KO mice on HFD, whereas there were no significant differences in lean body mass between genotypes. Although food intake was similar between genotypes, oxygen consumption was significantly increased in female KO mice on HFD. In addition, the uncoupling protein-1 expression in the brown adipose tissues was increased in KO mice. These data demonstrate that the absence of GR signaling in AgRP neurons resulted in increases in energy expenditure accompanied by decreases in adiposity in mice fed HFD, indicating that GR signaling in AgRP neurons suppresses energy expenditure under HFD conditions.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Shibata M,Banno R,Sugiyama M,Tominaga T,Onoue T,Tsunekawa T,Azuma Y,Hagiwara D,Lu W,Ito Y,Goto M,Suga H,Sugimura Y,Oiso Y,Arima Hdoi
10.1210/en.2015-1430subject
Has Abstractpub_date
2016-04-01 00:00:00pages
1457-66issue
4eissn
0013-7227issn
1945-7170journal_volume
157pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Fetal pancreatic islets (21.5 days old) were cultured in RPMI 1640 containing either 2.8 or 11.1 mM glucose for 7 days. After the 7-day culture period, islets cultured in 2.8 mM glucose demonstrated a minimal first phase of insulin secretion in response to acute glucose stimulation, whereas islets cultured in 11.1 mM ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-582
更新日期:1984-02-01 00:00:00
abstract::Phorbol ester receptors in GH4C1 rat pituitary cells have been studied using [3H]phorbol 12,13-dibutyrate (PDBu). Binding to intact cells was of higher affinity at 37 C than at 4 C (Kd, approximately 8 or approximately 34 nM, respectively). The dissociation rate (measured at 4 C) of PDBu from cells equilibrated at 37 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-6-2293
更新日期:1985-12-01 00:00:00
abstract::Although the increased expression of Igf-I in liver in response to GH is well characterized, the intracellular signaling pathways that mediate this effect have not been identified. Intracellular signaling molecules belonging to the Janus kinase-signal transducer and activator of transcription 5b (JAK2-STAT5b) pathway ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8400
更新日期:2001-09-01 00:00:00
abstract::The present study was undertaken to determine whether T3 could modify anterior pituitary Ca++ metabolism under basal conditions and in the presence of TRH. Paired hemipituitaries from hypothyroid rats were preincubated with T3 (10(-7) M) for 2 h, allowed to accumulate 45Ca++ for the third hour, and repeatedly washed i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1690
更新日期:1981-05-01 00:00:00
abstract::Uterine estrogen receptor (ER) and ER mRNA were measured in cycling and ovariectomized (OVX) estrogen-treated mice to probe the physiological regulation of the intracellular distribution and biosynthesis of ER. On proestrus, when plasma estradiol (E2) levels are highest, the cell nuclear ER concentration was 2.4-fold ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547720
更新日期:1992-04-01 00:00:00
abstract::Type I iodothyronine 5' deiodinase (5'DI) contains selenocysteine, encoded by a UGA codon, and this amino acid is essential for maximum catalytic efficiency in this enzyme. We recently showed that translation of UGA as selenocysteine in this protein requires a specific sequence of about 250 nucleotides in the 3' untra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1396330
更新日期:1992-10-01 00:00:00
abstract::Immunohistochemical localization of two estrogen receptor (ER) subtypes, ER beta and ER alpha, was performed in neonatal, early postnatal, immature, and adult rats to determine whether ER alpha and ER beta are differentially expressed in the ovary. ER beta and ER alpha were visualized using a polyclonal anti-ER beta a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6533
更新日期:1999-02-01 00:00:00
abstract::High density lipoprotein (HDL) participates in reverse cholesterol transport and in the delivery of cholesterol to steroid-producing tissues. Scavenger receptor class B type I (SR-BI) was recently shown to bind HDL and mediate internalization of its cholesterol content. We have cloned the rat homolog of this receptor,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.1.5666
更新日期:1998-01-01 00:00:00
abstract::Mature 31- and 34-kDa inhibin A and B negatively regulate the release of FSH from the anterior pituitary; however, a direct comparison of these hormones in vivo has not been undertaken. The bioactivities of highly purified preparations of recombinant human 31-kDa inhibin A and B were determined in rat pituitary cells ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1783
更新日期:2009-10-01 00:00:00
abstract::The effects of alloxan-induced diabetes on uterine decidualization and the associated changes in uterine blood flow (UBF) and corpus luteum activity were evaluated in rats between days 4-9 of pseudopregnancy (day 0 = ovulation). Rats were made diabetic (D) with a 40 mg/kg injection (iv) of alloxan on day 1 of pseudopr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-665
更新日期:1988-02-01 00:00:00
abstract::Recent evidence has linked mitochondrial dysfunction and DNA damage, increased oxidative stress in skeletal muscle, and insulin resistance (IR). The purpose of this study was to determine the role of the DNA repair enzyme, human 8-oxoguanine DNA glycosylase/apurinic/apyrimidinic lyase (hOGG1), on palmitate-induced mit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1076
更新日期:2013-08-01 00:00:00
abstract::Binding of atrial and brain natriuretic peptides to guanylyl cyclase-A/natriuretic peptide receptor-A produces second messenger cGMP, which plays an important role in maintaining renal and cardiovascular homeostasis. Mice carrying a targeted disruption of the Npr1 gene coding for guanylyl cyclase-A/natriuretic peptide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0655
更新日期:2010-12-01 00:00:00
abstract::We have investigated the effects of veratridine, a Na+ channel activator, and ouabain, an inhibitor of Na+-K+-ATPase, on short term (1-h) PRL release from primary cultures of rat anterior pituitary cells and from the rat anterior pituitary cell line GH4C1 in culture. Both compounds should increase intracellular Na+. V...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-4-1228
更新日期:1983-10-01 00:00:00
abstract::In rat uterus and prostate, 7 alpha, 17 alpha-dimethyl-19-nortestosterone (DMNT) binds to the androgen receptor specifically and with high affinity. However, this steroid does not bind to glucocorticoid receptors, since it does not displace binding of [3H]triamcinolone acetonide in calf thymus cytosol. In calf uterine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1327
更新日期:1986-04-01 00:00:00
abstract::A continuous flow incubation (perifusion) system was used to examine the effect of testosterone (T) and three of its 5alpha-reduced metabolites, dihydrotestosterone (DHT), 5alpha-androstane-3alpha, 17beta-diol (3alpha-Adiol) and its 3beta-epimer (3beta-Adiol) on LH and FSH release, induced by hypothalamic extract (HE)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-253
更新日期:1975-02-01 00:00:00
abstract::This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor messenger RNA, play specific roles during development of the ovarian follicle. In particular, we were interested in determining the effect of the ED-I (also te...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.6.6708
更新日期:1999-06-01 00:00:00
abstract::The two peptides pancreastatin and diazepam binding inhibitor (DBI) were recently demonstrated in pancreatic islets and were shown to inhibit insulin secretion in short term experiments. In the present study we investigated long term effects of pancreastatin and DBI on the DNA synthesis, polyamine content, and insulin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3277
更新日期:1991-06-01 00:00:00
abstract::The role of anti-Müllerian hormone (AMH) during gonad development has been studied extensively in many species of mammal, bird, reptile, and fish but remains unresolved in amphibians. In male mammalian embryos, Sox9 activates AMH expression, which initiates regression of the Müllerian ducts. However, Sox9 (Sry-related...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2053
更新日期:2015-05-01 00:00:00
abstract::The present study was conducted to examine the effect of LH on phospholipid metabolism in corpora luteal tissue. Collagenase-dispersed cells obtained from bovine corpora lutea of early pregnancy were incubated with 32PO4 in the presence or absence of LH and examined for their ability to incorporate this label into pho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-469
更新日期:1981-08-01 00:00:00
abstract::Chronic exposure to stressors impairs the function of multiple organ systems and has been implicated in increased disease risk. In the rodent, the chronic variable stress (CVS) paradigm has successfully modeled several stress-related illnesses. Despite striking disparities between men and women in the prevalence and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00217
更新日期:2018-07-01 00:00:00
abstract::The human estrogen receptor alpha (ERalpha) and the recently identified ERbeta share a high degree of amino acid homology; however, there are significant differences in regions of these receptors that would be expected to influence transcriptional activity. Consequently, we compared the mechanism(s) by which these rec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7179
更新日期:1999-12-01 00:00:00
abstract::11 beta-hydroxysteroid dehydrogenase 2 (11 beta-HSD2) protects mineralocorticoid receptors from high circulating levels of glucocorticoids and cDNAs for 11 beta-HSD2 from human and sheep have been recently cloned. We have isolated the gene for mouse 11 beta-HSD2, which spans 7 kilobases and is composed of 5 exons. In ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.10.7664690
更新日期:1995-10-01 00:00:00
abstract::GnRH neurons form the final common pathway regulating the secretion of gonadotropins from the anterior pituitary. Since the patterns of gonadotropin release display profound sexual dimorphism among mammals including the rodent, we undertook an ultrastructural analysis to determine whether these neurosecretory cells we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-695
更新日期:1990-02-01 00:00:00
abstract::This work examines the cellular pathophysiology associated with the weakened bone matrix found in a murine model of osteogenesis imperfecta murine (oim). Histomorphometric analysis of oim/oim bone showed significantly diminished bone mass, and the osteoblast and osteoclast histomorphometric parameters were increased i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8807
更新日期:2002-05-01 00:00:00
abstract::Evidence is presented that in vitamin D deficiency there are aberrations of estrogen metabolism in the laying hen. Five White Leghorn hens, 35 weeks of age, all laying soft shelled eggs as a result of complete withdrawal of supplemental vitamin D3 (D3) from the diet, were found to have a 3-fold higher molar ratio of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2170
更新日期:1984-12-01 00:00:00
abstract::The current study was designed to investigate the ability of prostaglandin F2 alpha (PGF2 alpha) to activate a second messenger system (phosphatidylinositol pathway) in corpora lutea (CL) of rhesus monkeys. Activation of this pathway was assessed by monitoring the hydrolysis of phosphatidylinositol to inositol phospha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.2.1639020
更新日期:1992-08-01 00:00:00
abstract::The actin-binding protein filamin A (FLNa) affects the intracellular trafficking of various classes of receptors and has a potential role in oncogenesis. However, it is unclear whether FLNa regulates the signaling capacity and/or down-regulation of the activated epidermal growth factor receptor (EGFR). Here it is show...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1344
更新日期:2009-06-01 00:00:00
abstract::The feedback sites of estrogen within the hypothalamus and lower brain stem involved in the suppression of pulsatile LH secretion during 48-h fasting were examined in ovariectomized rats with local estradiol (E2) implants. The animals were ovariectomized and immediately implanted stereotaxically with stainless steel c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070380
更新日期:1994-09-01 00:00:00
abstract::We tested further our postulate that enhanced de novo synthesis of phosphatidic acid is responsible for ACTH- and cAMP-induced increases in adrenal phospholipids in the phosphatidate polyphosphoinositide pathway. During incubation of adrenal sections or cells in vitro, ACTH and cAMP increased the concentrations of and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-1895
更新日期:1981-12-01 00:00:00
abstract::In vitro antiglucocorticoids (cortexolone and progesterone) were evaluated as in vivo antagonists of dexamethasone-induced increases in liver tyrosine amino transferase (TAT; EC 2.6.1.5), tryptophan oxygenase (TPO; EC 1.13.1.12), and glycogen deposition. Cortexolone antagonized the TPO and glycogen responses to dexame...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-1-117
更新日期:1980-07-01 00:00:00