Abstract:
:Proper development of the seminiferous tubules (or testis cords in embryos) is critical for male fertility. Sertoli cells, somatic components of the seminiferous tubules, serve as nurse cells to the male germline, and thus their numbers decide the quantity of sperm output in adulthood. We previously identified activin A, the protein product of the activin βA (Inhba) gene, as a key regulator of murine Sertoli cell proliferation and testis cord expansion during embryogenesis. Although our genetic studies implicated fetal Leydig cells as the primary producers of testicular activin A, gonocytes are another potential source. To investigate the relative contribution of gonocyte-derived activin A to testis morphogenesis, we compared testis development in the Inhba global knockout mouse, which lacks activin A production in all cells (including the gonocytes), and a steroidogenic factor 1 (Sf1)-specific conditional knockout model in which activin A expression in testicular somatic cells is disrupted but gonocyte expression of activin A remains intact. Surprisingly, testis development was comparable in these two models of activin A insufficiency, with similar reductions in Sertoli cell proliferation and minor differences in testis histology. Thus, our findings suggest activin A from male gonocytes is insufficient to promote Sertoli cell proliferation and testis cord expansion in the absence of somatic cell-derived activin A. Evaluation of adult male mice with fetal disruption of activin A revealed reduced testis size, lowered sperm production, altered testicular histology, and elevated plasma FSH levels, defects reminiscent of human cases of androgen-sufficient idiopathic oligozoospermia.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Archambeault DR,Tomaszewski J,Childs AJ,Anderson RA,Yao HHdoi
10.1210/en.2011-1288subject
Has Abstractpub_date
2011-11-01 00:00:00pages
4358-67issue
11eissn
0013-7227issn
1945-7170pii
en.2011-1288journal_volume
152pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Kisspeptins, products of the KiSS-1 gene with ability to bind G protein-coupled receptor 54 (GPR54), have been recently identified as major gatekeepers of reproductive function with ability to potently activate the GnRH/LH axis. Yet, despite the diversity of functional states of the female gonadotropic axis, pharmacol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1463
更新日期:2006-06-01 00:00:00
abstract::Kisspeptin neurons play an essential role in the regulation of fertility through direct regulation of the GnRH neurons. However, the relative contributions of the two functionally distinct kisspeptin neuron subpopulations to this critical regulation are not fully understood. Here we analyzed the specific projection pa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1131
更新日期:2015-07-01 00:00:00
abstract::Posttranslational processing of proadrenomedullin generates two biologically active peptides, adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP). Sequence comparison of homologous proadrenomedullin genes in vertebrate evolution shows a high degree of stability in the reading frame for AM, whereas P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0105
更新日期:2006-07-01 00:00:00
abstract::Physiological roles for PTH-related peptide (PTHrP) appear varied, but remain to be clarified. The peptide is present in large amounts in milk, and PTHrP mRNA has been shown to be present in high amounts in lactating mammary gland. Because PTHrP can cause smooth muscle relaxation, we hypothesized that the peptide migh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.8396010
更新日期:1993-09-01 00:00:00
abstract::Traditionally, it has been thought that the bioavailable fraction of circulating serum hormones, i.e. that which is available for cellular uptake and is physiologically active, is limited to the free (nonprotein bound) hormone. However, recent evidence, based on acute organ uptake of labeled hormone, suggests that the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1745
更新日期:1984-05-01 00:00:00
abstract::Five studies are reported which were designed to define the effects of an estrogen and a progestogen, alone and in combination, on tissue concentrations (heart, kidney, and liver) of two trace metals (zinc, copper) and four bulk cations (sodium, potassium, calcium, magnesium). Male rats received Premarin (0.08 mg) and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-5-1263
更新日期:1975-11-01 00:00:00
abstract::PRL gene expression in the anterior pituitary has been the focus of intensive investigation for many years, but very little information is available on the actual dynamics by which this process occurs in individual mammotrope cells. Here, we used single cell bioluminescent imaging microscopy and a recently refined rep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8682
更新日期:2002-03-01 00:00:00
abstract::The role of adrenergic stimulation in the regulation of mitogen-activated protein kinase (MAPK) in rat pinealocytes was investigated by measuring phosphorylated MAPK using Western blot analysis and a MAPK enzymatic assay. Stimulation with the endogenous neurotransmitter, norepinephrine (NE; a mixed alpha- and beta-adr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7856
更新日期:2000-12-01 00:00:00
abstract::Treatment of inflammation is often accomplished through the use of glucocorticoids. However, their use is limited by side effects. We have examined the activity of a novel glucocorticoid receptor ligand that binds the receptor efficiently and strongly represses inflammatory gene expression. This compound has potent an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1353
更新日期:2008-05-01 00:00:00
abstract::A detailed understanding of the mechanism of action of estrogen on bones is still lacking. The present study was designed to examine possible modulation by 17 beta-estradiol (E2) on the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and on vitamin D receptors (VDR) in the ROS 17/2.8 osteoblast-like cell line. Cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315250
更新日期:1992-05-01 00:00:00
abstract::We have recently demonstrated that 17beta-estradiol (E2) opposes cytokine-dependent increase of inducible nitric oxide synthase (iNOS) activity in rat smooth muscle cells and proposed that this effect might be associated to an antiinflammatory activity of this hormone. In the present study, we examine the E2 effects o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.4.7404
更新日期:2000-04-01 00:00:00
abstract::Intermittent PTH administration increases bone turnover, resulting in net anabolic effects on bone. These effects are primarily mediated by intracellular cAMP signaling. However, the molecular mechanisms that regulate PTH activity in bone remain incompletely understood. beta-Arrestin2, a G protein-coupled receptor reg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1282
更新日期:2005-04-01 00:00:00
abstract::To evaluate the relative contribution of the GH/IGF axis to the development of peak bone mineral density (BMD), we measured skeletal changes in IGF-I knockout (KO), IGF-II KO, and GH-deficient lit/lit mice and their corresponding control mice at d 23 (prepubertal), 31 (pubertal), and 56 (postpubertal) in the entire fe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220948
更新日期:2003-03-01 00:00:00
abstract::N-methyl-D,L,-aspartate (NMA), an analog of the excitatory neurotransmitter aspartate, has been previously shown to acutely stimulate LH release in the rodent and primate. In this study, we examine the effect of NMA on LH secretion in the long term ovariectomized adult rhesus monkey. After a 3-h control period, three ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-724
更新日期:1990-08-01 00:00:00
abstract::We previously reported the existence of a descending multisynaptic, pituitary-independent, neural pathway between the hypothalamus and the testes in the male rat. Stimulation of this pathway by the intracerebroventricular (icv) injection of IL-1beta or corticotropin-releasing factor blunts the testosterone (T) respons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1441
更新日期:2004-04-01 00:00:00
abstract::Bisphenol-A (BPA) is a component of polycarbonate and other plastics to which humans are regularly exposed at low levels. BPA is characterized as an endocrine disruptor because of observations of its estrogenic activity in various experimental models. We have previously shown evidence of disrupted hypothalamic feeding...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1718
更新日期:2017-04-01 00:00:00
abstract::Orally delivered salt stimulates renal salt excretion more effectively than does iv delivered salt. Although the mechanisms that underlie this "postprandial natriuresis" are poorly understood, the peptide uroguanylin (UGn) is thought to be a key mediator. However, the lack of selective assays for UGn gene products has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1725
更新日期:2008-09-01 00:00:00
abstract::Reproductive function is dependent on the interaction between GnRH and its cognate receptor found on gonadotrope cells of the anterior pituitary gland. GnRH activation of the GnRH receptor (GnRHR) is a potent stimulus for increased expression of multiple genes including the gene encoding the GnRHR itself. Thus, homolo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220784
更新日期:2003-03-01 00:00:00
abstract::A study was conducted to determine the role of the thyroid gland in three neuroendocrine responses to photoperiod; secretion of melatonin, PRL, and LH. Ewes were thyroidectomized (THX) in midsummer or left thyroid intact, and both groups were moved indoors to artificial short days (8 h of light, 16 h of darkness) for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013340
更新日期:1994-07-01 00:00:00
abstract::To begin to assess the function of the cell cycle regulator cyclin D3 during gametogenesis, the present study examined its expression, interacting partners, and associated kinase activity in the murine testis and ovary. In the early stages of postnatal testicular development, cyclin D3 protein was detected in spermato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.6.6756
更新日期:1999-06-01 00:00:00
abstract::The ALP1 [aci-reductone dioxygenase (ARD)-like protein 1] gene was identified in a comprehensive cDNA subtraction aimed at identifying genes regulated by androgens in the rat ventral prostate. ALP1 is homologous to the ARD/ARD' that were discovered in Klebsiella pneumoniae as enzymes that have the same polypeptide seq...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0947
更新日期:2004-04-01 00:00:00
abstract::Some TSH receptors (TSHR) on the cell surface cleave into A and B subunits. Cleavage at upstream Site 1 is followed by the proteolytic excision of an intervening C peptide region terminating at a downstream Site 2. Although present evidence suggests that Site 1 lies between amino acid residues 303 and 317, the mechani...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.10.7699
更新日期:2000-10-01 00:00:00
abstract::The development of acquired resistance to antihormonal agents in breast cancer is a major therapeutic problem. We have developed a tamoxifen-resistant (TAM-R) MCF-7 breast cancer cell line to investigate the mechanisms behind this condition. Both epidermal growth factor receptor (EGFR) and c-erbB2 mRNA and protein exp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220620
更新日期:2003-03-01 00:00:00
abstract::In Teleost fish, development, growth, and reproduction are influenced by the daily and seasonal variations of photoperiod and temperature. Early in vivo studies indicated the pineal gland mediates the effects of these external factors, most probably through the rhythmic production of melatonin. The present investigati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0707
更新日期:2003-10-01 00:00:00
abstract::Adrenomedullin (ADM) is a potent vasorelaxant peptide that plays important roles in cardiovascular homeostasis and inflammatory response. ADM derived from macrophages is one of the major sources of ADM that is produced in the inflammatory process. To assess the functions of ADM in inflammation, we studied the temporal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1080
更新日期:2005-03-01 00:00:00
abstract::Prokineticin-1 (PK1) is a recently described protein with a wide range of functions, including tissue-specific angiogenesis, modulation of inflammatory responses, and regulation of hemopoiesis. The aim of this study was to investigate the localization and expression of PK1 and PK receptor-1 (PKR1), their signaling pat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1695
更新日期:2008-07-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I) improves glucose metabolism and growth in patients with leprechaunism. We investigated signal transduction through IGF-I receptor in comparison with epidermal growth factor (EGF) receptor in early passages of cultured skin fibroblasts from a normal subject and a patient with leprec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4910
更新日期:1997-02-01 00:00:00
abstract::Mouse β-cell-specific reporter lines have played a key role in diabetes research. Although the rat provides several advantages, its use has lagged behind the mouse due to the relative paucity of genetic models. In this report we describe the generation and characterization of transgenic rats expressing a Renilla lucif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1550
更新日期:2016-03-01 00:00:00
abstract::Cytosols of whole testicular homogenates from the Syrian golden hamster contained specific binding sites for [3H]triamcinolone acetonide that exhibited limited capacity and high affinity binding characteristic of glucocorticoid receptors in other target tissues. The receptor complex sedimented as an 8.6S binder in low...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-5-1331
更新日期:1981-11-01 00:00:00
abstract::The effect of delta 9-tetrahydrocannabinol (THC) on suckling-induced oxytocin release was investigated by recording intramammary pressure changes in suckled rats treated iv with THC (0.5 mg/kg BW) or vehicle. Latency to the first posttreatment milk ejection and posttreatment milk ejection intervals and pressure wave a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-469
更新日期:1988-07-01 00:00:00