Abstract:
:PRL gene expression in the anterior pituitary has been the focus of intensive investigation for many years, but very little information is available on the actual dynamics by which this process occurs in individual mammotrope cells. Here, we used single cell bioluminescent imaging microscopy and a recently refined reporter gene strategy to measure PRL promoter-driven gene expression (PRL-GE) in individual living primary mammotropes. Using this approach we report a new phenomenon involving repetitive on/off gene expression bursts that occurred in a distinctly noncircadian oscillatory pattern. Furthermore, we demonstrate a functional basis for these gene expression oscillations, inasmuch as PRL-GE pulses were sensitive to calcium-dependent modulation, which we show arose exclusively as changes in the shape of individual pulse episodes. Our results provide the first clear evidence that PRL-GE, in its homologous cell environment, displays oscillatory bursts of activity. Moreover, they strongly support the idea that these discrete on/off bursts of activity serve as an important determinant of the timing and level of PRL-GE under both basal and stimulated conditions.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Shorte SL,Leclerc GM,Vazquez-Martinez R,Leaumont DC,Faught WJ,Frawley LS,Boockfor FRdoi
10.1210/endo.143.3.8682subject
Has Abstractpub_date
2002-03-01 00:00:00pages
1126-33issue
3eissn
0013-7227issn
1945-7170journal_volume
143pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Multiple forms of PRL differing in their physicochemical and biological characteristics have been described. We have analyzed the molecular forms of human (h) PRL released in culture by pure hPRL-secreting tumors with a particular attention to glycosylated hPRL. The prolactinoma cells from six different tumors release...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2667
更新日期:1988-06-01 00:00:00
abstract::Nuclear receptor function on DNA is regulated by the balanced recruitment of coregulatory complexes. Recruited proteins that increase gene expression are called coactivators, and those that decrease gene expression are called corepressors. Little is known about the role of corepressors, such as nuclear receptor corepr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0594
更新日期:2010-03-01 00:00:00
abstract::Immunohistochemical (immunoperoxidase) studies were performed on 478 sections from 97 rat pituitary glands with rabbit antisera to unconjugated human synthetic calcitonin beta-endorphin, and/or ACTH-(17--39). Calcitonin-positive cells were present in a majority of the anterior lobes studied, whereas they were present ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1966
更新日期:1980-06-01 00:00:00
abstract::Activins and transforming growth factor-beta (TGF beta) are crucial autocrine, paracrine, and endocrine modulators of anterior pituitary function. Activins regulate most pituitary cells and lactotropes are targets of TGF beta. Smad2 and Smad3 are two cellular mediators of activin/TGF beta signaling, whereas Smad7 is a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8028
更新日期:2001-03-01 00:00:00
abstract::Melanocortin receptor 4 (MCR4) and CB(1) cannabinoid receptors independently modulate food intake. Although an interaction between the cannabinoid and melanocortin systems has been found in recovery from hemorrhagic shock, the interaction between these systems in modulating food intake has not yet been examined. The p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0059
更新日期:2004-07-01 00:00:00
abstract::We have recently identified gonadotropes as target cells for ATP action via ATP receptors of the P2U subtype. The present studies have used gonadotrope-derived alpha T3-1 cells to examine the possible signaling mechanisms subserving ATP action in gonadotropes. Addition of ATP produced a biphasic intracellular Ca2+ (Ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536620
更新日期:1996-01-01 00:00:00
abstract::Progesterone released by cumulus cells surrounding the egg induces a Ca2+ influx into human sperm cells via the cationic channel of sperm (CatSper) Ca2+ channel and controls multiple Ca2+-dependent responses essential for fertilization. We hypothesized that chemical UV filters may mimic the physiological action of pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1473
更新日期:2016-11-01 00:00:00
abstract::Glucocorticoid feedback inhibition at the level of the brain is extremely complex, involving feedback at both hypothalamic and suprahypothalamic levels. The hippocampus has been implicated as a suprahypothalamic mediator of such feedback, based on numerous lesion, stimulation, and steroid implantation studies. These r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-6-2881
更新日期:1989-12-01 00:00:00
abstract::It is known that the circadian rhythm in hepatic phosphoenolpyruvate carboxykinase expression (a limiting catalytic step of gluconeogenesis) and hepatic glucose production is maintained by both daily oscillation in autonomic inputs to the liver and night feeding behavior. However, increased glycemia and reduced melato...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1088
更新日期:2011-04-01 00:00:00
abstract::Lanosterol 14alpha-demethylase (CYP51) responds to cholesterol feedback regulation through sterol regulatory element binding proteins (SREBPs). The proximal promoter of CYP51 contains a conserved region with clustered regulatory elements: GC box, cAMP-response elements (CRE-like), and sterol regulatory element (SRE). ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0781
更新日期:2005-12-01 00:00:00
abstract::The mechanism of the inhibitory effect of long term treatment with estradiol on dopamine synthesis in tuberoinfundibular dopaminergic (TIDA) neurons was studied by using hypothalamic slices from ovariectomized rats. Treatment with 2 mg estradiol valerate (EV) at a 3-week interval increased the weight of the anterior p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-692
更新日期:1987-08-01 00:00:00
abstract::Enhanced epinephrine secretion from adrenal chromaffin cells (ACCs) is an important homeostatic response to severe systemic inflammation during sepsis. Evidence suggests that increased activation of ACCs by preganglionic sympathetic neurons and direct alterations in ACC function contribute to this response. However, t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1623
更新日期:2014-01-01 00:00:00
abstract::In the prostate, testosterone action depends on conversion to bioactive metabolites dihydrotestosterone and 17beta-estradiol (E2) via the 5alpha-reductase and aromatase enzymes, respectively. Exogenous estrogen inhibits prostate growth by indirect effects caused by suppression of pituitary gonadotropins and testicular...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220493
更新日期:2002-12-01 00:00:00
abstract::The direct actions of kit-ligand/stem cell factor (KL) in developing ovarian follicles were investigated. Previous studies have shown that granulosa cells express KL that can support oocyte development. The current study demonstrates that KL can also act directly on theca cells to promote cellular growth and different...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5368
更新日期:1997-09-01 00:00:00
abstract::The conceptus and endometrium secrete large amounts of prostaglandin E₂ (PGE₂) into the porcine uterine lumen during the periimplantation period. We hypothesized that PGE₂ acts on conceptus/trophoblast cells through auto- and paracrine mechanisms. Real-time RT-PCR analysis revealed that PGE₂ receptor (PTGER)2 mRNA was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2271
更新日期:2013-10-01 00:00:00
abstract::A study was conducted to determine the role of the thyroid gland in three neuroendocrine responses to photoperiod; secretion of melatonin, PRL, and LH. Ewes were thyroidectomized (THX) in midsummer or left thyroid intact, and both groups were moved indoors to artificial short days (8 h of light, 16 h of darkness) for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013340
更新日期:1994-07-01 00:00:00
abstract::Regulation of the number of pituitary vasopressin (VP) receptors plays an important role in controlling pituitary responsiveness during alterations of the hypothalamic pituitary adrenal axis. The mechanisms regulating these VP receptors were studied by analysis of the effects of adrenalectomy and glucocorticoid admini...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5580
更新日期:1997-12-01 00:00:00
abstract::Induction of cyclin proteins is required for progression of cells through the G(1)-S and G(2)-M cell cycle checkpoints and is a primary mechanism by which mitogens regulate cell cycle progression. IGF-I and the epidermal growth factor (EGF)-related ligands are mitogens for mammary epithelial cells in vitro and are ess...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8774
更新日期:2002-05-01 00:00:00
abstract::Small amounts of TRH are present in the thyroid, and TRH has been shown in several studies to have a moderate direct inhibitory effect on TSH-stimulated thyroid hormone secretion. In a recent publication, however, data were presented to demonstrate that very low doses of TRH given in short pulses induce immediate incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-2-385
更新日期:1988-02-01 00:00:00
abstract::The aldo-keto reductase 1B7 (AKR1B7) encodes an aldose-reductase that has been reported as a detoxification enzyme until now. We have demonstrated that AKR1B7 is differently expressed in various mouse white adipose tissues depending on their location. Its expression is associated with a higher ratio of preadipocytes v...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1707
更新日期:2007-05-01 00:00:00
abstract::Experiments were conducted to obtain information on the possible transfer of TSH and thyroid hormones from mother rats to their infants via milk. The mothers were thyroidectomized by surgical or chemical (tapazole) means to eliminate maternal thyroid hormones from milk while increasing its content of TSH. Their pups w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-800
更新日期:1983-03-01 00:00:00
abstract::Chorionic gonadotropin (CG) is an early embryo-derived signal that is known to support the corpus luteum. An in vivo baboon model was used to study the direct actions of human CG (hCG) on the endometrium, during the periimplantation period. Endometrial gene expression was analyzed using microarrays. The endometrial bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0832
更新日期:2007-02-01 00:00:00
abstract::GH is considered to play a role in the pathogenesis of diabetic retinopathy, causing neovascularization in the retina. The present study was conducted to assess the possibility that GH may play a role in ocular development by determining whether GH is expressed in the eye of the chicken during development. In the 17-d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8552
更新日期:2001-12-01 00:00:00
abstract::Oxytocin (OT) has a dual action in the uterus: a uterotonic action on myometrial cells and a prostaglandin (PG)-releasing action on endometrial/decidual cells. It had not been determined whether the OT-binding sites or receptors on the myometrial and the endometrial/decidual membranes are of the same type or may repre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.8382600
更新日期:1993-03-01 00:00:00
abstract::Pituitary growth hormone (GH) content and plasma GH response to pentobarbital (PB) of severely hypothyroid rats are markedly decreased compared with euthyroid rats. Both pituitary and plasma GH may be increased by single ip injections of physiologic doses of thyroxine (T4) (1.75 mug/100g BW) or triiodothyronine (T3) (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-1-77
更新日期:1976-01-01 00:00:00
abstract::Hypothalamic insulin signaling is essential to the maintenance of glucose and energy homeostasis. During pathological states, such as obesity and type 2 diabetes mellitus, insulin signaling is impaired. One key mechanism involved in the development of insulin resistance is lipotoxicity, through increased circulating s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1122
更新日期:2010-02-01 00:00:00
abstract::The effect of the administration of sheep anti-LHRH gamma-globulin (anti-LHRHG) on the maintenance of pregnancy was investigated in rats. Nidation was confirmed by laparotomy on day 7 or 8 of pregnancy. In one experiment, rats were then injected iv with 1 ml anti-LHRHG or normal sheep gamma-globulin (NSG) daily from d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-4-1024
更新日期:1976-04-01 00:00:00
abstract::A neutral protease with angiotensin I-forming activity which could readily be separated from acid proteases and plasma and renal renin was obtained from extracts of dog brain. This enzyme has an apparent mol wt of 40,000 by Sephadex chromatography. On chromatofocusing, it displays isoelectric points of 7.92, 7.73, and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-6-2210
更新日期:1984-06-01 00:00:00
abstract::Five studies are reported which were designed to define the effects of an estrogen and a progestogen, alone and in combination, on tissue concentrations (heart, kidney, and liver) of two trace metals (zinc, copper) and four bulk cations (sodium, potassium, calcium, magnesium). Male rats received Premarin (0.08 mg) and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-5-1263
更新日期:1975-11-01 00:00:00
abstract::It is unclear whether the maturation of corticotrophs from the fetal to the adult type in the fetal sheep pituitary in late gestation is associated with changes in the sensitivity of the fetal pituitary to corticotrophic secretagogues and in the form of ACTH-containing peptides (IR-ACTH) secreted into the circulation....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1942
更新日期:1991-10-01 00:00:00