Effects of desamino-(1-3)-insulin-like growth factor I on bone cell function in rat calvarial cultures.


:Insulin-like growth factor (IGF) I, a polypeptide synthesized by skeletal cells, and its amino terminus truncated derivative desamino-(1-3)-IGF I (des-IGF I) were compared for their effects on bone formation in vitro. Des-IGF I and IGF I were studied for their effects on DNA and collagen synthesis in cultures of intact fetal rat calvariae and of osteoblast-enriched (Ob) cells from fetal rat parietal bone, and for their ability to bind to IGF receptors in Ob cells and to IGF binding proteins (IGF-BPs) from calvariae. Des-IGF I and IGF I increased [3H] thymidine incorporation into DNA, [3H]proline incorporation into collagen and noncollagen protein, and the mitotic index in intact calvariae. Both factors had similar actions in calvariae. Des-IGF I stimulated all parameters studied at 1 nM, and IGF I was effective on the labeling of DNA at 1 nM, but concentrations of 10 nM were required to observe changes in collagen and noncollagen protein synthesis and in the mitotic index. The effect of des-IGF I on collagen synthesis was independent from that on DNA synthesis, as it is known for IGF I, and both forms of IGF I were equally potent for their inhibitory effects on collagen degradation in calvarial cultures. In Ob cells, neither des-IGF I nor IGF I altered the incorporation of [3H]thymidine into DNA, but both factors at 10-100 nM increased [3H]proline incorporation into collagen to a similar extent. Receptor studies revealed a similar binding capacity for des-IGF I and IGF I to the IGF I receptor(s) in Ob cells, although at 0.2 nM des-IGF I was slightly more effective than IGF I. In contrast, des-IGF I was 100-fold less effective than IGF I for its ability to bind to partially purified IGF-BPs from cultured calvariae. In conclusion, des-IGF I enhances calvarial DNA and collagen synthesis and osteoblastic collagen synthesis to a somewhat greater extent than IGF I, in spite of a much lesser affinity for IGF-BPs.






Canalis E,McCarthy TL,Centrella M




Has Abstract


1991-07-01 00:00:00












  • Fetal programming: prenatal testosterone excess leads to fetal growth retardation and postnatal catch-up growth in sheep.

    abstract::Alterations in the maternal endocrine, nutritional, and metabolic environment disrupt the developmental trajectory of the fetus, leading to adult diseases. Female offspring of rats, subhuman primates, and sheep treated prenatally with testosterone (T) develop reproductive/metabolic defects during adult life similar to...


    pub_type: 杂志文章


    authors: Manikkam M,Crespi EJ,Doop DD,Herkimer C,Lee JS,Yu S,Brown MB,Foster DL,Padmanabhan V

    更新日期:2004-02-01 00:00:00

  • Overexpression of glutamic acid decarboxylase-67 (GAD-67) in gonadotropin-releasing hormone neurons disrupts migratory fate and female reproductive function in mice.

    abstract::gamma-Aminobutyric acid (GABA) inhibits the embryonic migration of GnRH neurons and regulates hypothalamic GnRH release. A subset of GnRH neurons expresses GABA along their migratory route in the nasal compartment before entering the brain, suggesting that GABA produced by GnRH neurons may help regulate the migratory ...


    pub_type: 杂志文章


    authors: Heger S,Seney M,Bless E,Schwarting GA,Bilger M,Mungenast A,Ojeda SR,Tobet SA

    更新日期:2003-06-01 00:00:00

  • The 17alpha and 17beta isomers of estradiol both induce rapid spine synapse formation in the CA1 hippocampal subfield of ovariectomized female rats.

    abstract::Previous studies have demonstrated that estradiol-17beta and estradiol-17alpha both induce short-latency effects on spatial memory in rats, estradiol-17alpha being at least as potent as its 17beta isomer. To determine whether the mechanisms underlying these behavioral responses might include effects on hippocampal syn...


    pub_type: 杂志文章


    authors: MacLusky NJ,Luine VN,Hajszan T,Leranth C

    更新日期:2005-01-01 00:00:00

  • Possible mechanism of action of metoclopramide-induced aldosterone secretion: in vivo and in vitro studies in the sheep.

    abstract::To define further the mechanism by which metoclopramide, a dopamine antagonist, stimulates aldosterone secretion, seven lambs were injected iv with metoclopramide during a constant infusion of 5% dextrose in water and again during a constant infusion of trimethapan, a ganglionic blocker. In addition, suspensions of ad...


    pub_type: 杂志文章


    authors: Wilson TA,Kaiser DL,Peach MJ,Wright EM,Carey RM

    更新日期:1983-09-01 00:00:00

  • Genomic structure and characterization of the 5'-flanking region of the human ghrelin gene.

    abstract::Ghrelin, an endogenous ligand for the GH secretagogue receptor, induces GH secretion, food intake, and positive energy balance. Although ghrelin exhibits a variety of hormonal actions, the mechanisms regulating ghrelin expression and secretion remain unclear. To understand regulation of human ghrelin gene expression, ...


    pub_type: 杂志文章


    authors: Kanamoto N,Akamizu T,Tagami T,Hataya Y,Moriyama K,Takaya K,Hosoda H,Kojima M,Kangawa K,Nakao K

    更新日期:2004-09-01 00:00:00

  • Dexamethasone does not inhibit antidiuretic hormone-stimulated prostaglandin production in vivo.

    abstract::Glucocorticoids are potent inhibitors of prostaglandin (PG) release in vitro, but they fail to influence basal PG production in vivo. To test one possible explanation for this apparent discrepancy in the present study we investigated the effect of dexamethasone (DEX) on basal and antidiuretic hormone-stimulated renal ...


    pub_type: 杂志文章


    authors: Náray-Fejes-Tóth A,Fejes-Tóth G

    更新日期:1986-12-01 00:00:00

  • Induction of estrogen and progesterone receptors and decidualization in the hamster uterus by cholera toxin.

    abstract::Cholera toxin (CT) injected ip on day 1 (day of ovulation) of the 4-day hamster estrous cycle, when circulatory progesterone is high and estrogen low, induced a massive uterine decidual reaction, a progesterone-dependent growth normally triggered by the implanting blastocyst. However, CT injected ip on day 3, when cir...


    pub_type: 杂志文章


    authors: Alleva JJ,Kenimer JG,Jordan AW,Lamanna C

    更新日期:1983-06-01 00:00:00

  • Bradykinin stimulates aldosterone release from cultured bovine adrenocortical cells through bradykinin B2 receptors.

    abstract::The adrenal cortex contains a kallikrein-like enzyme that may lead to bradykinin (BK) formation. This study was designed to determine whether BK acts on adrenocortical cells to stimulate steroid secretion. BK, Lys-BK, a specific BK 2 (B2) receptor agonist, and desArg9-BK, a specific BK 1 (B1) receptor agonist, all sti...


    pub_type: 杂志文章


    authors: Rosolowsky LJ,Campbell WB

    更新日期:1992-04-01 00:00:00

  • Potential involvement of the carboxy-terminus of the Glut 1 transporter in glucose transport.

    abstract::The role of the carboxy-terminal domain of the Glut 1 glucose transporter was investigated using an antipeptide antibody to the C-terminal part of the molecule. The study was performed in fibroblasts transfected with the cDNA coding for the human insulin receptor. These cells acutely respond to insulin for glucose tra...


    pub_type: 杂志文章


    authors: Tanti JF,Gautier N,Cormont M,Baron V,Van Obberghen E,Le Marchand-Brustel Y

    更新日期:1992-11-01 00:00:00

  • Developmental study of androgen responsiveness in the submandibular gland of the mouse.

    abstract::The development of androgen responsiveness in the submandibular gland of normal and androgen-resistant (Tfm) mice of different ages was studied after varying hormonal treatments. Total esteroproteolytic (tamase) activity of submandibular gland homogenates was used as a marker for androgen action. Newborn mice of all f...


    pub_type: 杂志文章


    authors: Dunn JF,Wilson JD

    更新日期:1975-06-01 00:00:00

  • Insulin-like growth factor-binding protein-3 production by MCF-7 breast cancer cells: stimulation by retinoic acid and cyclic adenosine monophosphate and differential effects of estradiol.

    abstract::The insulin-like growth factors (IGFs) stimulate the proliferation of human breast cancer cells, including the estrogen-dependent cell line MCF-7. These cells secrete regulatory IGF-binding proteins (IGFBPs) which may enhance or attenuate IGF-stimulated cell proliferation. In this study, we have used RIA to quantify t...


    pub_type: 杂志文章


    authors: Martin JL,Coverley JA,Pattison ST,Baxter RC

    更新日期:1995-03-01 00:00:00

  • Almost exclusive androgenic action of dehydroepiandrosterone in the rat mammary gland.

    abstract::To determine the relative role of the androgenic and/or estrogenic components of the action of dehydroepiandrosterone (DHEA) on the histomorphology and structure of the rat mammary gland, ovariectomized (OVX) female animals received DHEA administered alone or in combination with the pure antiandrogen flutamide or the ...


    pub_type: 杂志文章


    authors: Sourla A,Martel C,Labrie C,Labrie F

    更新日期:1998-02-01 00:00:00

  • Stimulatory effect of insulin-like growth factor-1 on renal Pi transport and plasma 1,25-dihydroxyvitamin D3.

    abstract::Administration of GH increases both the tubular reabsorption of inorganic phosphate (Pi) and the plasma level of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. These two effects could be induced by a common mediator, possibly the GH-generated insulin-like growth factor 1 (IGF-1). In the present work, the influence of recomb...


    pub_type: 杂志文章


    authors: Caverzasio J,Montessuit C,Bonjour JP

    更新日期:1990-07-01 00:00:00

  • Unique properties of the follicle-stimulating hormone- and cholera toxin-sensitive adenylyl cyclase of immature granulosa cells.

    abstract::Previous studies have shown that the adenylyl cyclase of intact granulosa cells from immature porcine follicles has a uniquely prolonged responsiveness to FSH and an atypically poor responsiveness to cholera toxin relative to other cells. The present studies were designed to determine if these characteristics were due...


    pub_type: 杂志文章


    authors: Hunzicker-Dunn M,LaBarbera AR

    更新日期:1986-01-01 00:00:00

  • Platelet-derived growth factor stimulates bone resorption by monocyte monolayers.

    abstract::Resorption of devitalized bone particles by monocytes grown in culture was stimulated by platelet-derived growth factor (PDGF) in a dose-dependent manner. Bone resorption in response to PDGF was time-dependent with a significant increase over control cultures evident by 72 hours. These data are the first to demonstrat...


    pub_type: 杂志文章


    authors: Key LL Jr,Carnes DL Jr,Weichselbaum R,Anast CS

    更新日期:1983-02-01 00:00:00

  • Differential gene expression of cytochrome P450 11beta-hydroxylase in rat adrenal cortex after in vivo activation.

    abstract::In situ hybridization histochemistry was used to monitor the expression of 3beta-hydroxysteroid dehydrogenase, delta4-isomerase (3betaHSD) and cytochrome P450 11beta-hydroxylase (P45011beta) messenger RNA (mRNA) in adult rat adrenals after stimulation in vivo. In Exp 1, adrenals were collected from rats injected with ...


    pub_type: 杂志文章


    authors: Engeland WC,Levay-Young BK,Rogers LM,Fitzgerald D

    更新日期:1997-06-01 00:00:00

  • Thyrotropin-induced acceleration of calcium efflux from mouse thyroid: evidence for inhibition by excess iodide.

    abstract::We investigated the effect of TSH on calcium transport in mouse thyroid, as well as the influence of iodide thereupon. Thyroid lobes were incubated in Krebs-Ringer bicarbonate buffer containing [45Ca2+] and the time-dependent uptake of [45Ca2+] by the lobes was observed. In the presence of 0.5 mU/ml TSH, the uptake of...


    pub_type: 杂志文章


    authors: Hashizume K,Ichikawa K,Komiya I,Onaya T

    更新日期:1984-05-01 00:00:00

  • Proestrous hormone surges dissociated from ovulation in the estrogen-treated hamster.

    abstract::Hamsters injected sc with 50 mug estradiol cyclopentylpropionate (ECP) at 0900 h on day 1 (metestrus) of the cycle show the normal "postovulatory" vaginal discharge 4 days later but do not resume ovulation until 14--18 days later. After ECP injection ovarian histology reveals that the follicles normally destined to ov...


    pub_type: 杂志文章


    authors: Greenwald GS

    更新日期:1975-10-01 00:00:00

  • Two promoters mediate transcription from the human LHX3 gene: involvement of nuclear factor I and specificity protein 1.

    abstract::The LHX3 transcription factor is required for pituitary and nervous system development in mammals. Mutations in the human gene are associated with hormone-deficiency diseases. The gene generates two mRNAs, hLHX3a and hLHX3b, which encode three proteins with different properties. Here, the cis elements and trans-acting...


    pub_type: 杂志文章


    authors: Yaden BC,Garcia M 3rd,Smith TP,Rhodes SJ

    更新日期:2006-01-01 00:00:00

  • Glucagon-like peptide-1 regulates the beta cell transcription factor, PDX-1, in insulinoma cells.

    abstract::Glucagon-like peptide-1 (GLP-1) enhances insulin biosynthesis and secretion as well as transcription of the insulin, GLUT2 and glucokinase genes. The latter are also regulated by the PDX-1 homeoprotein. We investigated the possibility that GLP-1 may be having its long-term pleiotropic effects through a hitherto unknow...


    pub_type: 杂志文章


    authors: Wang X,Cahill CM,Piñeyro MA,Zhou J,Doyle ME,Egan JM

    更新日期:1999-10-01 00:00:00

  • The antigenic epitopes of human grown hormone as mapped by monoclonal antibodies.

    abstract::The antigenic epitopes of human GH (hGH) have been investigated using monoclonal antibodies (Mabs) to hGH. A panel of 12 Mabs was incorporated into two-site binding assays in order to investigate the antigenic surface of hGH. Nine reaction patterns were observed. The Mabs were further characterized in terms of their c...


    pub_type: 杂志文章


    authors: Strasburger CJ,Kostyo J,Vogel T,Barnard GJ,Kohen F

    更新日期:1989-03-01 00:00:00

  • Hormonal regulation of rabbit uteroglobin gene transcription.

    abstract::Synthesis of uteroglobin in rabbit uterine epithelial cells during early pregnancy is induced by progesterone and apparently suppressed by estrogen. To assess whether the steroid hormones exert regulatory effects on the expression of the uteroglobin gene, we performed transcriptional rate assays in endometrial nuclei ...


    pub_type: 杂志文章


    authors: Shen XZ,Tsai MJ,Bullock DW,Woo SL

    更新日期:1983-03-01 00:00:00

  • How much of the gonadotropin-releasing hormone (GnRH) surge is required for generation of the luteinizing hormone surge in the ewe? Duration of the endogenous GnRH signal.

    abstract::The preovulatory LH surge in the sheep is accompanied by a massive and sustained surge of GnRH. The objective of this study was to examine the duration of the endogenous GnRH signal required to induce and maintain a LH surge of full amplitude and duration. For this purpose, we assessed the effect of a competitive GnRH...


    pub_type: 杂志文章


    authors: Evans NP,Dahl GE,Caraty A,Padmanabhan V,Thrun LA,Karsch FJ

    更新日期:1996-11-01 00:00:00

  • Minireview: SLCO and ABC transporters: a role for steroid transport in prostate cancer progression.

    abstract::Androgens play a critical role in the development and progression of prostate cancer (PCa), and androgen deprivation therapy via surgical or medical castration is front-line therapy for patients with advanced PCa. However, intratumoral testosterone levels are elevated in metastases from patients with castration-resist...


    pub_type: 杂志文章,评审


    authors: Cho E,Montgomery RB,Mostaghel EA

    更新日期:2014-11-01 00:00:00

  • Induction of type II 5'-deiodinase activity in cultured rat astroglial cells by 12-O-tetradecanoylphorbol-13-acetate: dependence on glucocorticoids.

    abstract::The effect of an activator of protein kinase C, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the 5'-deiodinase (5'D) activity was studied in rat astroglial cells cultured in chemically defined medium. TPA promoted a large increase in the type II 5'D activity, which was maximal 5-10 h after addition of TPA and then d...


    pub_type: 杂志文章


    authors: Courtin F,Chantoux F,Gavaret JM,Toru-Delbauffe D,Jacquemin C,Pierre M

    更新日期:1989-09-01 00:00:00

  • Induction of Ad4BP/SF-1, steroidogenic acute regulatory protein, and cytochrome P450scc enzyme system expression in newly established human granulosa cell lines.

    abstract::We have established immortalized human granulosa cells by triple transfection of primary cells obtained from in vitro fertilization patients with SV40 DNA, Ha-ras oncogene, and a temperature sensitive (ts) mutant of the tumor suppressor gene p53 (p53val135). Forty-one clones were isolated, and their steroidogenic resp...


    pub_type: 杂志文章


    authors: Hosokawa K,Dantes A,Schere-Levy C,Barash A,Yoshida Y,Kotsuji F,Vlodavsky I,Amsterdam A

    更新日期:1998-11-01 00:00:00

  • Patterns of cellular peptide synthesis by cultured bovine granulosa cells.

    abstract::The electrophoretic distribution of the polypeptides synthesized by bovine granulosa cell cultures after metabolic labeling with [35S]methionine has been analyzed by double gel electrophoresis. The fluctuations of 35 polypeptides have been followed as a function of the size of the follicles from which cultures origina...


    pub_type: 杂志文章


    authors: Savion N,Gospodarowicz D

    更新日期:1980-12-01 00:00:00

  • Stimulation by bombesin of immunoreactive somatostatin release into rat hypophysial portal blood.

    abstract::Bombesin was injected into the cerebral ventricle of male rats anesthetized with urethane to study its effect on plasma levels of immunoreactive somatostatin (IRS) in hypophysial portal and jugular blood. An intraventricular injection of bombesin (0.2 and 2 micrograms/rat) caused a significant and dose-related increas...


    pub_type: 杂志文章


    authors: Abe H,Chihara K,Minamitani N,Iwasaki J,Chiba T,Matsukura S,Fujita T

    更新日期:1981-07-01 00:00:00

  • A Nonradioactive Uptake Assay for Rapid Analysis of Thyroid Hormone Transporter Function.

    abstract::Thyroid hormones (TH) are actively taken up into target cells via TH-transmembrane transporters (THTT). Their activity and expression patterns define a layer of endocrine regulation that is poorly understood. Therefore, THTT are potential targets for interfering agents (endocrine disruptors) as well as for pharmacolog...


    pub_type: 杂志文章


    authors: Jayarama-Naidu R,Johannes J,Meyer F,Wirth EK,Schomburg L,Köhrle J,Renko K

    更新日期:2015-07-01 00:00:00

  • Corticotropin-releasing factor-overexpressing mice exhibit reduced neuronal activation in the arcuate nucleus and food intake in response to fasting.

    abstract::Corticotropin-releasing factor (CRF) overexpressing (OE) mice are a genetic model that exhibits features of chronic stress. We investigated whether the adaptive feeding response to a hypocaloric challenge induced by food deprivation is impaired under conditions of chronic CRF overproduction. Food intake response to a ...


    pub_type: 杂志文章


    authors: Stengel A,Goebel M,Million M,Stenzel-Poore MP,Kobelt P,Mönnikes H,Taché Y,Wang L

    更新日期:2009-01-01 00:00:00