Abstract:
:Although GH plays a key role in postnatal growth and differentiation, its role in fetal differentiation is not clear at the present. The aim of the present study was to investigate whether GH plays a role in fetal sexual differentiation, and we used in vitro organ culture assay of sexual differentiation to determine this. The results showed that anti-rGH antibody blocked Wolffian duct differentiation specifically in the presence of fetal testes. Exogenous GH supplemented in the above experiment reversed the blocking effect of anti-GH. Among the other related products, insulin-like growth factor I was highly effective in reversing the anti-GH effect, insulin-like growth factor II was partially effective, but PRL was unable to reverse the anti-GH effect. GH itself was found to produce some masculinizing effect, as demonstrated by its ability to stabilize the Wolffian duct in female fetuses. The role of GH was further demonstrated by the observation that GH-immunoreactive material of the size of authentic GH was detected in the 18-day fetal reproductive tract, and the concentration of this material increased in response to progression of sexual differentiation. Determination of androgen-binding activity using Scatchard analysis on the cells isolated from the 18-day fetal reproductive tract indicated that androgen-binding activity increased after GH treatment of the cells. Thus, it may be concluded that GH influences male sexual differentiation and alters the androgen-binding activity of the fetal reproductive tract.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Nguyen AP,Chandorkar A,Gupta Cdoi
10.1210/endo.137.9.8756530subject
Has Abstractpub_date
1996-09-01 00:00:00pages
3659-66issue
9eissn
0013-7227issn
1945-7170journal_volume
137pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::PTH is an important peptide hormone regulator of calcium homeostasis and osteoblast function. However, its mechanism of action in osteoblasts is poorly understood. Our previous study demonstrated that PTH activates mouse osteocalcin (Ocn) gene 2 promoter through the osteoblast-specific element 1 site, a recently ident...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1573
更新日期:2008-04-01 00:00:00
abstract::The physicochemical and immunological properties of androgen receptors from kidney and brain of testicular-feminized (Tfm) mutant mice and wild-type mice were compared. Analysis by gel filtration and sucrose density gradients revealed that the mol wt of the mutant receptor was 66K (38A; 3.8S) which was significantly s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-771
更新日期:1989-02-01 00:00:00
abstract::Task3 K(+) channels are highly expressed in the adrenal cortex and contribute to the angiotensin II and K(+) sensitivity of aldosterone-producing glomerulosa cells. Adult Task3(-/-) mice display a partially autonomous aldosterone secretion, subclinical hyperaldosteronism, and salt-sensitive hypertension. Here, we inve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1101
更新日期:2013-08-01 00:00:00
abstract::MCF-7 cells migrate through vitronectin-coated filters in response to insulin-like growth factor I (IGF-I); migration is inhibited by the matrix metalloproteinase (MMP) inhibitor BB-94, but not by the serine proteinase inhibitor aprotinin. MMP-9 was identified in the conditioned medium of MCF-7 cells; in addition, flu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6623
更新日期:1999-04-01 00:00:00
abstract::Primary cultures of enzymatically dispersed rat anterior pituitary cells were used to examine the effect of pure 31 kilodalton bovine inhibin on GnRH-induced up-regulation of GnRH binding sites. After 2 days in culture, the cells were exposed to stimuli with or without test substances for 10 h, followed by evaluation ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-363
更新日期:1989-01-01 00:00:00
abstract::Primary functional bovine adrenal cortical cell cultures have been developed to study the factors controlling adrenal cell growth. Cells were prepared by the collagenase technique and maintained in F-12 medium containing fetal calf serum and horse serum. Cells contained abundant lipid as demonstrated by staining with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-4-1080
更新日期:1977-04-01 00:00:00
abstract::Human thyroperoxidase (hTPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis and plays a major role in thyroid autoimmunity. Whereas hormonosynthesis occurs at the apical membrane of thyroid cells, TPO localizes mainly in the perinuclear membrane and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.10.6265
更新日期:1998-10-01 00:00:00
abstract::The mechanism by which the proto-oncogene, c-fos, is up-regulated in response to PGE2 in the mouse osteoblastic (MC3T3-E1) cell line was investigated using RT-PCR. c-fos messenger RNA up-regulation by dmPGE2 is rapid, starting 10 min post stimulation, and transient. The specific protein kinase A (PKA) inhibitor, H89, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7271
更新日期:2000-01-01 00:00:00
abstract::Effect of cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) on [125I]T3 binding to nuclei was investigated in vitro. CTBP and nuclei were prepared from rat kidney. CTBP was inactivated by incubating with charcoal and activated by incubating with NADPH or with NADP and dithiothreitol. Two complexes of CT...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2851
更新日期:1989-06-01 00:00:00
abstract::Extracts of rat pancreas contain significant amounts of an [3H]estradiol-binding protein. The amount of steroid-binding activity that could be measured varied considerably depending on the tonicity of the homogenizing medium. High speed supernatants of homogenates initially prepared in isotonic buffer contained about ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2221
更新日期:1985-06-01 00:00:00
abstract::To explore the role of transforming growth factor-beta (TGF beta) isoforms and other growth-related genes during prostate morphogenesis in the mouse, we examined mRNA levels in fetal day 17 urogenital sinus, mesenchyme (UGM), and epithelium (UGE) as well as in the ventral, dorsal, and anterior lobes of the adult prost...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119140
更新日期:1994-03-01 00:00:00
abstract::This study examined the requirement for 17 beta-estradiol (E) and relaxin (R) during the preparturient period (approximately the last 30 h of gestation) for normal parturition and pup survival in the rat. On the evening of day 21 of pregnancy, primiparous Sprague-Dawley rats were bilaterally ovariectomized or sham ope...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1958
更新日期:1988-05-01 00:00:00
abstract::Treatment of 5-day cyclic rats with 10 or 100 microgram estradiol benzoate on the day of estrus induced a luteal phase in all animals studied. On the other hand, an injection with 1 microgram estradiol benzoate given on the same day failed to induce pseudopregnancy. When 10 mg progesterone were injected on the day of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-1-195
更新日期:1979-07-01 00:00:00
abstract::Sertoli cells regulate differentiation and development of the testis and are essential for maintaining adult testis function. To model the effects of dysregulating Sertoli cell number during development or aging, we have used acute diphtheria toxin-mediated cell ablation to reduce Sertoli cell population size. Results...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00196
更新日期:2017-09-01 00:00:00
abstract::We characterized four antipeptide polyclonal antibodies (abs) able to specifically recognize each thyroid hormone receptor (TR) isoform. The abs immunoprecipitated both the in vitro synthesized receptor and the receptor expressed in E. coli and their specificity was confirmed by competition studies and immunohistochem...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1425440
更新日期:1992-11-01 00:00:00
abstract::A parotid hormone (PH-A beta) has been isolated and purified from porcine parotid glands. The biological effect of the hormone is the stimulation of a dentinal fluid transport (DFT) in molar teeth of rats. The method for purifying the PH-A beta includes: extraction at pH 7 and 5, ultrafiltration through Amicon membran...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1994
更新日期:1980-06-01 00:00:00
abstract::The present study examined the effects of FSH and insulin (IN) on 17 beta-estradiol (E2) secretion and granulosa cell proliferation. For these studies, immature rat ovaries were maintained in perifusion culture and continuously exposed to FSH (0-800 ng RP-1 eq/ml) and /or IN (0.2 U/ml). At specific times, the ovaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-191
更新日期:1991-01-01 00:00:00
abstract::Osteoblasts are known to produce osteoclast-stimulating activity (OSA). The aim of the current study was to relate the expression of OSA to the osteoblastic phenotype and examine its regulation by calciotropic hormones. The study was performed with the normal osteoblastic cell clone CRP 10/30 and the preosteoblastic c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.3.1505460
更新日期:1992-09-01 00:00:00
abstract::The PRL-stimulating effect of metoclopramide (2-methoxy-5-chloroprocainamide) was examined in normal and adrenalectomized male rats pretreated with DL-p-chlorophenylalanine methyl ester (PCPA), an inhibitor of serotonin biosynthesis. PCPA significantly reduced the hypothalamic content of serotonin, increased basal PRL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1622
更新日期:1981-05-01 00:00:00
abstract::Steroid hormones can differentially modify gonadotropin release stimulated by GnRH. Decreased GnRH-mediated gonadotropin release has been observed in vitro after pretreatment with androgens or glucocorticoids. In this study, we tested this phenomenon further with the use of a new cytochemical stain for a potent biotin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-1-396
更新日期:1985-07-01 00:00:00
abstract::19-Nor progesterone (19-nor P) has previously been shown to have a approximately 3-fold higher affinity for mineralocorticoid receptors than progesterone (P), its C-19 methylated parent steroid; in contrast, 19-nor aldosterone has less than 1% the mineralocorticoid receptor affinity of aldosterone. In the present stud...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-313
更新日期:1981-07-01 00:00:00
abstract::The two phases of the ovulatory menstrual cycle of the primate are characterized by divergent activities of the GnRH pulse generator. During the luteal phase, LH pulse frequency is significantly reduced below that observed during the follicular phase. In this report we investigate whether the decrease in pulse frequen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-2044
更新日期:1987-05-01 00:00:00
abstract::We tested further our postulate that enhanced de novo synthesis of phosphatidic acid is responsible for ACTH- and cAMP-induced increases in adrenal phospholipids in the phosphatidate polyphosphoinositide pathway. During incubation of adrenal sections or cells in vitro, ACTH and cAMP increased the concentrations of and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-1895
更新日期:1981-12-01 00:00:00
abstract::The role of GH during the ovine estrous cycle is unknown. In this study, pituitary GH mRNA and peripheral serum GH levels were measured in ewes representing the major phases of the estrous cycle. There was a significant rise (P less than 0.05) in both GH mRNA and serum GH concentrations during the follicular phase of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1474
更新日期:1989-09-01 00:00:00
abstract::Immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. The bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-1-63
更新日期:1975-01-01 00:00:00
abstract::Recent research progress indicates a close link between ghrelin, a natural ligand of GH secretagogues receptor (GHS-R), and both the metabolic balance and body composition. To clarify the involvement of ghrelin and GHS-R in the process of adipogenesis, we measured the expression of GHS-R and peroxisome proliferator-ac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220783
更新日期:2003-03-01 00:00:00
abstract::To date, the role of pancreatic hormones in pancreatic islet growth and differentiation is poorly understood. To address this issue, we examined mice with a disruption in the gene encoding prohormone convertase 2 (PC2). These mice are unable to process proglucagon, prosomatostatin, and other neuroendocrine precursors ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0088
更新日期:2003-09-01 00:00:00
abstract::The relationship between progesterone and prostaglandin (PG) secretion in the pseudopregnant rabbit corpus luteum was investigated using isolated in vitro perfused ovaries. Progesterone and PG secretion were measured on days 1, 11, and 18 of hCG-induced pseudopregnancy. The mean progesterone secretion increased signif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1198
更新日期:1989-03-01 00:00:00
abstract::To ascertain if reductions in fetal plasma cortisol cause increases in fetal plasma ACTH, we treated pregnant ewes or their fetuses with aminoglutethimide (10 mg/kg BW) and metyrapone (20 mg/kg BW) and measured the hormonal responses with RIAs. When given to fetuses (n = 9) at 0.90 +/- 0.01 gestation (term-145 days), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1307
更新日期:1988-09-01 00:00:00
abstract::The localization of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the adrenal gland of the frog, Rana ridibunda, was examined by the indirect immunofluorescence technique. Using an antiserum directed against rat alpha-CGRP, the presence of a network of positive fibers was observed in the adrenal pare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013380
更新日期:1994-07-01 00:00:00