Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor.


:Primary functional bovine adrenal cortical cell cultures have been developed to study the factors controlling adrenal cell growth. Cells were prepared by the collagenase technique and maintained in F-12 medium containing fetal calf serum and horse serum. Cells contained abundant lipid as demonstrated by staining with Oil Red O and showed strongly positive staining for delta5,3beta-hydroxysteroid dehydrogenase. ACTH inhibited DNA synthesis and stimulated steriodogenesis in these cells. Fibroblast growth factor (FGF) was shown to be a potent stimulator of the growth of normal bovine adrenal cortical cells maintained in tissue culture. The minimal effective dose of FGF was 1 ng/ml with maximal effects being observed at 100 ng/ml. The effect of FGF was dependent on the serum concentration. Inclusion of FGF in F-12 medium containing serum permitted cloning of functional bovine adrenal cortical cells from cultures seeded at low density (4 cells/cm2). ACTH inhibited the mitogenic effects of FGF. In addition to its mitogenic action, FGF is a migratory factor for bovine adrenal cortical cells. Though ACTH inhibited the mitogenic effects of FGF, it did not block the migratory activity. Epidermal growth factor did not affect the growth of either normal bovine adrenal or functional mouse adrenal tumor cells (Y-1) in tissue culture. FGF is the first direct mitogen identified for adrenal cortical cells; ACTH opposes this mitogenic action and functions directly as a differentiate function signal.






Gospodarowicz D,Ill CR,Hornsby PJ,Gill GN




Has Abstract


1977-04-01 00:00:00












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    pub_type: 杂志文章


    authors: Liu X,Brown RS,Herbison AE,Grattan DR

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  • Novel intronic promoter in the rat ER alpha gene responsible for the transient transcription of a variant receptor.

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    pub_type: 杂志文章


    authors: Tiffoche C,Vaillant C,Schausi D,Thieulant ML

    更新日期:2001-09-01 00:00:00

  • 17 beta-estradiol, but not 5 alpha-dihydrotestosterone, augments antibodies to double-stranded deoxyribonucleic acid in nonautoimmune C57BL/6J mice.

    abstract::The influence of sex hormones on the immune response to foreign antigens as well as self-antigens is now recognized. In this study, we investigated the influence of gender and sex hormones on the development of antibodies to double-stranded DNA in nonautoimmune C57BL/6J mice. Immunoglobulin G (IgG) anti-dsDNA antibodi...


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    authors: Verthelyi D,Ahmed SA

    更新日期:1994-12-01 00:00:00

  • Short ghrelin peptides neither displace ghrelin binding in vitro nor stimulate GH release in vivo.

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    pub_type: 杂志文章


    authors: Canalis E

    更新日期:1985-03-01 00:00:00

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    authors: Naulé L,Maione L,Kaiser UB

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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