Follicle-stimulating hormone and insulin regulation of 17 beta-estradiol secretion and granulosa cell proliferation within immature rat ovaries maintained in perifusion culture.


:The present study examined the effects of FSH and insulin (IN) on 17 beta-estradiol (E2) secretion and granulosa cell proliferation. For these studies, immature rat ovaries were maintained in perifusion culture and continuously exposed to FSH (0-800 ng RP-1 eq/ml) and /or IN (0.2 U/ml). At specific times, the ovaries were removed from perifusion, and the perifusate was assayed for E2 by RIA. The ovaries were then incubated with [3H]thymidine ([3H]T) in order to estimate granulosa cel mitotic activity. FSH increased E2 secretion in a dose-dependent manner (P less than 0.05), but did not enhance [3H]T incorporation (P greater than 0.05) after 48 h of perifusion. Conversely, IN increased [3H]T incorporation (P less than 0.05) without stimulating E2 secretion (P greater than 0.05) after 48 h of perifusion. FSH alone or in combination with IN stimulated [3H]T incorporation at 24 h of perifusion compared to both zero hour control (P less than 0.05) and IN treatments (P less than 0.05). The inability of FSH to stimulate [3H]T incorporation after 48 h did not appear to be due to increased E2, since IN stimulated [3H]T incorporation in the presence of 100 ng E2/ml. Further, continuous exposure to FSH was required to maintain E2 secretion, demonstrating that after 24 h, FSH loses its capacity to stimulate granulosa cell [3H]T incorporation, but not E2 secretion. Finally, when ovaries are pretreated with FSH for 48 h and then exposed to FSH and/or IN, [3H]T incorporation was stimulated, and E2 secretion inhibited. These data suggest that 1) initially, FSH acts to stimulate mitosis then promotes granulosa cell E2 secretion; and 2) once granulosa cells begin to secrete E2 they are still capable of mitosis, but their mitotic activity is no longer directed by FSH.






Peluso JJ,Delidow BC,Lynch J,White BA




Has Abstract


1991-01-01 00:00:00












  • Prenatal Dexamethasone Exposure Induced Ovarian Developmental Toxicity and Transgenerational Effect in Rat Offspring.

    abstract::Prenatal dexamethasone exposure (PDE) induces multiorgan developmental toxicities in offspring. Here we verified the transgenerational inheritance effect of ovarian developmental toxicity by PDE and explored its intrauterine programming mechanism. Pregnant rats subcutaneously received 0.2 mg/kg/d dexamethasone from ge...


    pub_type: 杂志文章


    authors: Lv F,Wan Y,Chen Y,Pei L,Luo D,Fan G,Luo M,Xu D,Wang H

    更新日期:2018-03-01 00:00:00

  • Arsenite modulates cardiac substrate preference by translocation of GLUT4, but not CD36, independent of mitogen-activated protein kinase signaling.

    abstract::The protein thiol-modifying agent arsenite, a potent activator of stress signaling, was used to examine the involvement of MAPKs in the regulation of cardiac substrate uptake. Arsenite strongly induced p38 MAPK phosphorylation in isolated rat cardiac myocytes but also moderately enhanced phosphorylation of p42/44 ERK ...


    pub_type: 杂志文章


    authors: Luiken JJ,Momken I,Habets DD,El Hasnaoui M,Coumans WA,Koonen DP,Glatz JF,Bonen A

    更新日期:2006-11-01 00:00:00

  • Diametric effects of bacterial endotoxin lipopolysaccharide on adrenal and Leydig cell steroidogenic acute regulatory protein.

    abstract::Immune activation results in the activation of adrenal steroidogenesis and inhibition of gonadal steroidogenesis. Previous studies indicated that these effects were caused primarily by activation and suppression of the secretion of ACTH and LH, respectively. However, other evidence indicated a direct effect of the imm...


    pub_type: 杂志文章


    authors: Hales KH,Diemer T,Ginde S,Shankar BK,Roberts M,Bosmann HB,Hales DB

    更新日期:2000-11-01 00:00:00

  • Localization of immunoreactive androgen in testicular tissue.

    abstract::Immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. The bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horse...


    pub_type: 杂志文章


    authors: Bubenik GA,Brown GM,Grota LJ

    更新日期:1975-01-01 00:00:00

  • Striated muscle fibers differentiate in primary cultures of adult anterior pituitary cells.

    abstract::Anterior pituitary cells from adult male rats were cultured on a natural extracellular matrix (ECM). From the 5th day, spindle-shaped cells, fusing to form elongated fibers, were observed among the epithelial cells. These fibers later increased in size and number and were identified as striated muscle fibers, based on...


    pub_type: 杂志文章


    authors: Spira O,Atzmon R,Rahamim E,Bar-Shavit R,Gross J,Gordon A,Vlodavsky I

    更新日期:1988-06-01 00:00:00

  • Cerebellar binding of avian pancreatic polypeptide.

    abstract::Studies were carried out to determine the regional central nervous system and species specificity of the previously observed [125I]iodoavian pancreatic polypeptide ( [125I]iodo-APP) specific binding to chick brain membranes. The avian species examined were chicken, pigeon, duck, quail, chukar, and pheasant. In all spe...


    pub_type: 杂志文章


    authors: Adamo ML,Hazelwood RL

    更新日期:1984-03-01 00:00:00

  • Anti-Müllerian Hormone Is a Marker for Chemotherapy-Induced Testicular Toxicity.

    abstract::Due to increased numbers of young cancer patients and improved survival, the impact of anticancer treatments on fertility has become a major health concern. Despite mounting research on ovarian toxicity, there is paucity of data regarding reliable biomarkers of testicular toxicity. Our aim was to evaluate anti-Mülleri...


    pub_type: 杂志文章


    authors: Levi M,Hasky N,Stemmer SM,Shalgi R,Ben-Aharon I

    更新日期:2015-10-01 00:00:00

  • Functional differences between the outer and inner zones of the guinea pig adrenal cortex.

    abstract::The guinea pig adrenal cortex is grossly composed of two regions: an outer, yellow zone and an inner, brown zone. These zones, which represent 33% and 66% of the total adrenocortical volume, respectively, can be separated by blunt dissection. It has been previously reported that specific pregnenolone and pregnenolone ...


    pub_type: 杂志文章


    authors: Strott CA,Goff AK,Lyons CD

    更新日期:1981-12-01 00:00:00

  • The immature rat ovary is innervated by vasoactive intestinal peptide (VIP)-containing fibers and responds to VIP with steroid secretion.

    abstract::The nature and role of the peptidergic innervation of the ovary were examined by determining the location and function of vasoactive intestinal peptide (VIP)-containing nerve fibers in the immature rat ovary. Immunohistofluorescence analysis of prepubertal ovaries using a specific VIP antibody revealed sparse delicate...


    pub_type: 杂志文章


    authors: Ahmed CE,Dees WL,Ojeda SR

    更新日期:1986-04-01 00:00:00

  • Discordant effects of aging on prolactin and luteinizing hormone-beta messenger ribonucleic acid levels in the female rat.

    abstract::To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LH beta mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the correspondi...


    pub_type: 杂志文章


    authors: Stewart DA,Blackman MR,Kowatch MA,Danner DB,Roth GS

    更新日期:1990-02-01 00:00:00

  • Mice lacking beta-adrenergic receptors have increased bone mass but are not protected from deleterious skeletal effects of ovariectomy.

    abstract::Activation of beta2-adrenergic receptors inhibits osteoblastic bone formation and enhances osteoclastic bone resorption. Whether beta-blockers inhibit ovariectomy-induced bone loss and decrease fracture risk remains controversial. To further explore the role of beta-adrenergic signaling in skeletal acquisition and res...


    pub_type: 杂志文章


    authors: Bouxsein ML,Devlin MJ,Glatt V,Dhillon H,Pierroz DD,Ferrari SL

    更新日期:2009-01-01 00:00:00

  • Endocrine modulation of gamma-aminobutyric acidergic innervation in the rat fallopian tube.

    abstract::The present study investigates the effect of different endocrine manipulations on the gamma-aminobutyric acid (GABA)-ergic system in the rat fallopian tube. Either hypophysectomy or ovariectomy induced a significant decrease of glutamic acid decarboxylase (GAD) activity and of GABA levels in in situ tubes. This effect...


    pub_type: 杂志文章


    authors: Celotti F,Apud JA,Melcangi RC,Masotto C,Tappaz M,Racagni G

    更新日期:1986-01-01 00:00:00

  • Direct regulation of GnRH neuron excitability by arcuate nucleus POMC and NPY neuron neuropeptides in female mice.

    abstract::Hypothalamic neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons act to sense and coordinate the brain's responses to metabolic cues. One neuronal network that is very sensitive to metabolic status is that controlling fertility. In this study, we investigated the impact of neuropeptides released by NPY and POM...


    pub_type: 杂志文章


    authors: Roa J,Herbison AE

    更新日期:2012-11-01 00:00:00

  • Polymorphism of prolactin secreted by human prolactinoma cells: immunological, receptor binding, and biological properties of the glycosylated and nonglycosylated forms.

    abstract::Multiple forms of PRL differing in their physicochemical and biological characteristics have been described. We have analyzed the molecular forms of human (h) PRL released in culture by pure hPRL-secreting tumors with a particular attention to glycosylated hPRL. The prolactinoma cells from six different tumors release...


    pub_type: 杂志文章


    authors: Pellegrini I,Gunz G,Ronin C,Fenouillet E,Peyrat JP,Delori P,Jaquet P

    更新日期:1988-06-01 00:00:00

  • A high affinity gonadotropin-releasing hormone (GnRH) tracer, radioiodinated at position 6, facilitates analysis of mutant GnRH receptors.

    abstract::Cloning of GnRH receptors from several animal species has made it possible to investigate receptor function using site-directed mutagenesis. However, many mutant GnRH receptors exhibit decreased ligand binding, which makes analysis of their ligand binding characteristics technically difficult. To increase the affinity...


    pub_type: 杂志文章


    authors: Flanagan CA,Fromme BJ,Davidson JS,Millar RP

    更新日期:1998-10-01 00:00:00

  • Solubilization, purification, and partial characterization of thyrotropin receptor from bovine and human thyroid glands.

    abstract::In an attempt to purify the thyroid receptor for TSH and to study interaction with the thyroid-stimulating antibody (TSAb) of graves' disease, we used both bovine and human thyroid glands. With either tissue, the 10,000 X g pellet of homogenized material was solubilized with 0.5% Triton N-101; excess Triton was remove...


    pub_type: 杂志文章


    authors: Koizumi Y,Zakarija M,McKenzie JM

    更新日期:1982-04-01 00:00:00

  • Triiodothyronine receptors in rat lung.

    abstract::Specific triiodothyronine receptor activity has been detected in rat lung nuclei isolated by Triton X-100, which was sensitive to pH and exhibited temperature-dependent association kinetics. This activity represented a single order of high affinity binding sites, with an apparent dissociation constant for 3,3',5-triio...


    pub_type: 杂志文章


    authors: Morishige WK,Guernsey DL

    更新日期:1978-05-01 00:00:00

  • Androgens decrease LHRH binding sites in rat anterior pituitary cells in culture.

    abstract::[125I]-[D-Ser(TBU)6]LHRH-EA binds to a single class of high affinity sites in rat anterior pituitary cells in culture at an apparent dissociation constant of 0.25 nM at 0-4C. The order of potency of a representative group of LHRH agonists and antagonists to displace the labeled ligand is similar to their LH-releasing ...


    pub_type: 杂志文章


    authors: Giguere V,Lefebvre FA,Labrie F

    更新日期:1981-01-01 00:00:00

  • Antiglucocorticoids: in vivo assay and evaluation of cortexolone, progesterone, and 6-beta-bromoprogesterone.

    abstract::In vitro antiglucocorticoids (cortexolone and progesterone) were evaluated as in vivo antagonists of dexamethasone-induced increases in liver tyrosine amino transferase (TAT; EC, tryptophan oxygenase (TPO; EC, and glycogen deposition. Cortexolone antagonized the TPO and glycogen responses to dexame...


    pub_type: 杂志文章


    authors: Naylor PH,Gilani SS,Milholland RJ,Rosen F

    更新日期:1980-07-01 00:00:00

  • 17β-Estradiol and 17α-Ethinyl Estradiol Exhibit Immunologic and Epigenetic Regulatory Effects in NZB/WF1 Female Mice.

    abstract::17α-Ethinyl estradiol (EE), a synthetic analog of natural estrogen 17β-estradiol (E2), is extensively used in hormonal contraceptives and estrogen replacement therapy, and it has also been found in sewage effluents. Given that E2 is a well-known immunomodulator, surprisingly there has been only limited information on ...


    pub_type: 杂志文章


    authors: Dai R,Edwards MR,Heid B,Ahmed SA

    更新日期:2019-01-01 00:00:00

  • Biological characterization of a heterodimer-selective retinoid X receptor modulator: potential benefits for the treatment of type 2 diabetes.

    abstract::Specific retinoid X receptor (RXR) agonists, such as LG100268 (LG268), and the thiazolidinedione (TZD) PPARgamma agonists, such as rosiglitazone, produce insulin sensitization in rodent models of insulin resistance and type 2 diabetes. In sharp contrast to the TZDs that produce significant increases in body weight gai...


    pub_type: 杂志文章


    authors: Leibowitz MD,Ardecky RJ,Boehm MF,Broderick CL,Carfagna MA,Crombie DL,D'Arrigo J,Etgen GJ,Faul MM,Grese TA,Havel H,Hein NI,Heyman RA,Jolley D,Klausing K,Liu S,Mais DE,Mapes CM,Marschke KB,Michellys PY,Montrose-Rafi

    更新日期:2006-02-01 00:00:00

  • In vitro maturation of steroidogenic capacity of ovine fetal and neonatal adrenal cells.

    abstract::The in vitro production of total corticosteroids, corticosterone (B), and cortisol (F) by cultured adrenocortical cells from fetuses (115-120 days old) and newborn lambs exposed to ACTH-(1-24) was investigated in relation to the time of culture. Corticosteroids were measured by using an antibody against F, whereas B a...


    pub_type: 杂志文章


    authors: Durand P,Cathiard AM,Saez JM

    更新日期:1984-04-01 00:00:00

  • Neurons that migrate from the olfactory epithelium in the chick express luteinizing hormone-releasing hormone.

    abstract::In several mammalian species, luteinizing hormone-releasing hormone (LHRH) neurons have been shown to migrate from nasal regions to the brain during early development. Using immunocytochemistry, we have identified LHRH containing neurons in developing chick embryos. In embryonic day 4 (E4) and E5 animals, a small grou...


    pub_type: 杂志文章


    authors: Norgren RB Jr,Lehman MN

    更新日期:1991-03-01 00:00:00

  • Differential regulation of insulin-like growth factor-binding protein-3 protease activity in MCF-7 breast cancer cells by estrogen and transforming growth factor-beta1.

    abstract::We have examined the regulation of an insulin-like growth factor-binding protein-3 (IGFBP-3) protease secreted by MCF-7 human breast cancer cells using a ligand-binding assay that relies on the decrease in affinity for des(1-3)IGF-I that occurs when IGFBP-3 becomes proteolyzed. IGFBP-3 protease activity was not altere...


    pub_type: 杂志文章


    authors: Salahifar H,Baxter RC,Martin JL

    更新日期:2000-09-01 00:00:00

  • Differential effects of atrial natriuretic factor on angiotensin II- and adrenocorticotropin-stimulated aldosterone secretion.

    abstract::The possible role of atrial natriuretic factor (ANF) in the regulation of adrenal sensitivity to angiotensin II (AII) was investigated in vivo and in vitro by analyzing the characteristics of the inhibitory effect of ANF on aldosterone production stimulated by AII and other stimuli. In isolated adrenal glomerulosa cel...


    pub_type: 杂志文章


    authors: Aguilera G

    更新日期:1987-01-01 00:00:00

  • Conversion of L-thyroxine to triiodo-L-thyronine and biological activity of L-thyroxine, as measured by changes in growth hormone.

    abstract::Pituitary growth hormone (GH) content and plasma GH response to pentobarbital (PB) of severely hypothyroid rats are markedly decreased compared with euthyroid rats. Both pituitary and plasma GH may be increased by single ip injections of physiologic doses of thyroxine (T4) (1.75 mug/100g BW) or triiodothyronine (T3) (...


    pub_type: 杂志文章


    authors: Hervás F,Morreale de Escobar G,Escobar del Rey F

    更新日期:1976-01-01 00:00:00

  • Delayed mammary gland involution in mice with mutation of the insulin-like growth factor binding protein 5 gene.

    abstract::IGFs (IGF-I and IGF-II) are essential for development, and their bioactivities are tightly regulated by six related IGF-binding proteins (IGFBPs). IGFBP-5 is the most highly conserved binding protein and is expressed in several key developmental lineages as well as in multiple adult tissues including the mammary gland...


    pub_type: 杂志文章


    authors: Ning Y,Hoang B,Schuller AG,Cominski TP,Hsu MS,Wood TL,Pintar JE

    更新日期:2007-05-01 00:00:00

  • Effect of K+ deprivation and ouabain upon glucagon release.

    abstract::The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (...


    pub_type: 杂志文章


    authors: Leclercq-Meyer V,Marchand J,Malaisse WJ

    更新日期:1983-07-01 00:00:00

  • Longitudinal Metabolic Impacts of Perinatal Exposure to Phthalates and Phthalate Mixtures in Mice.

    abstract::Developmental exposures to phthalates are suspected to contribute to risk of metabolic syndrome. However, findings from human studies are inconsistent, and long-term metabolic impacts of early-life phthalate and phthalate mixture exposures are not fully understood. Furthermore, most animal studies investigating metabo...


    pub_type: 杂志文章


    authors: Neier K,Cheatham D,Bedrosian LD,Gregg BE,Song PXK,Dolinoy DC

    更新日期:2019-07-01 00:00:00

  • Sex differences in the metabolic effects of testosterone in sheep.

    abstract::Adiposity is regulated in a sexually divergent manner. This is partly due to sex steroids, but the differential effects of androgens in males and females are unclear. We investigated effects of testosterone on energy balance in castrated male (n = 6) and female sheep (n = 4), which received 3 × 200 mg testosterone imp...


    pub_type: 杂志文章


    authors: Clarke SD,Clarke IJ,Rao A,Cowley MA,Henry BA

    更新日期:2012-01-01 00:00:00