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Electrical responses of rat islets maintained in culture with varying levels of glucose.

Abstract：

:The electrical responses of rat islet cells maintained in culture with varying levels of glucose were determined. After culture with 2.8 or 4.2 mM glucose, the beta-cells did not respond electrically to 27.8 mM glucose. However, after culture in 5.6 mM glucose, the cells responded to 27.8 mM glucose in short term experiments with depolarization and an increase in the incidence of spike activity. Islet cells maintained in 8.4 or 14.0 mM glucose had increasingly greater (negative) membrane potentials. The incidence of glucose-induced spike activity was the greatest for cells which had been cultured in 14.0 mM glucose. Raising glucose in the culture medium from 2.8 to 14.0 mM for the last 2 days of culture restored the ability of the islets to respond electrically to glucose in short term experiments. These observations show that the electrical phenomena of the beta cell membrane are affected by alterations in the level of glucose in the culture medium and serve as reliable indicators of functional glucose sensitivity of the islet cells.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Pace CS,Conant S,Lacy PE

doi

10.1210/endo-102-5-1350

subject

Has Abstract

pub_date

1978-05-01 00:00:00

pages

1350-5

issue

5

eissn

0013-7227

issn

1945-7170

journal_volume

102

pub_type

杂志文章

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