Abstract:
:The influence of GH and several other pituitary hormones on the secretion and intracellular content of immunoreactive somatostatin (IRS) in cultured hypothalamic cells was examined. Hypothalamic cells prepared from 17-day-old rat embryos and maintained as reaggregate monolayers for 12 days in vitro were used. Short term release of IRS from these cultures was found to be stable and relatively constant. IRS release was enhanced by 60 mM K+ in a calcium-dependent fashion and by dopamine at concentrations as low as 1 nM. GH (1 microM) increased medium IRS content above baseline 12, 24, and 48 h after the incubation was begun, but had no effect after only 4 h. Hypothalamic cell content of IRS was increased by a 24-h incubation in 0.3 and 1.0 microM GH, but not by 0.1 microM GH. TSH (1 microM) induced an increase in IRS release comparable to 1 microM GH. However, intracellular IRS content was decreased after exposure to TSH. Cosyntropin, an ACTH analog, inhibited both IRS release and cell content, whereas PRL had no effect on either medium or cellular IRS content. Our results demonstrate that developing somatostatinergic neurons in the hypothalamus have the capacity to respond to pituitary hormones, indicating that short-loop feedback pathways may exist in perinatal hypothalamic cells.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Robbins RJ,Leidy JW Jr,Landon RMdoi
10.1210/endo-117-2-538subject
Has Abstractpub_date
1985-08-01 00:00:00pages
538-43issue
2eissn
0013-7227issn
1945-7170journal_volume
117pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The mechanism by which xylazine raises plasma glucose but lowers insulin concentrations was examined in Holstein cows. Intravenous injection of xylazine (15-150 micrograms/kg) induced a dose-dependent hyperglycemia and hypoinsulinemia for 3-4 h. An alpha 2-adrenergic blocking agent, yohimbine, and an alpha 1- and alph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-3-825
更新日期:1981-09-01 00:00:00
abstract::The vertebrate adenohypophysis forms as a placode at the anterior margin of the neural plate, requiring both hedgehog (Hh) and fibroblast growth factor (Fgf) mediated cell-cell signaling for induction and survival of endocrine cell types. Using small molecule inhibitors to modulate signaling levels during zebrafish de...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0315
更新日期:2008-09-01 00:00:00
abstract::The existence of cytoplasmic estrogen receptors (ERs) has been reported in the guinea pig brain using immunocytochemical techniques. While cytoplasmic ERs have been reported recently in other species, such as opossum, musk shrew, and ferrets, an exclusively cell nuclear pattern of ER immunoreactivity has been reported...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.3.1380440
更新日期:1992-09-01 00:00:00
abstract::Type 1 diabetes is caused by autoimmune destruction of β-cells. Although immunotherapy can restore self-tolerance thereby halting continued immune-mediated β-cell loss, residual β-cell mass and function is often insufficient for normoglycemia. Using a growth factor to boost β-cell mass can potentially overcome this ba...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1549
更新日期:2016-01-01 00:00:00
abstract::Humanin is a peptide that is cytoprotective against stresses in many cell types. We investigated whether a potent humanin analogue S14G-humanin (HNG) would protect against chemotherapy-induced damage to normal cells without interfering with the chemotherapy-induced suppression of cancer cells. Young adult male mice we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1542
更新日期:2015-12-01 00:00:00
abstract::The action of excitatory amino acid agonists on endogenous somatostatin release was examined in primary cultures of rat diencephalic neurons. Increasing concentrations of glutamate stimulated somatostatin release in a dose-dependent manner. Since this effect was decreased by Mg2+, all experiments were performed in Mg2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-5-2360
更新日期:1988-11-01 00:00:00
abstract::Immunoreactive beta-endorphin (ir-beta EP) and immunoreactive N-acetyl-endorphin (ir-NacEP) have been demonstrated in the rat thyroid by specific RIA and characterized by reverse phase HPLC. In addition, pituitary and thyroid ir-beta EP and ir-NacEP levels have been determined after manipulation of the pituitary-thyro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-642
更新日期:1986-08-01 00:00:00
abstract::The localization of calcitonin gene-related peptide (CGRP)-like immunoreactivity in the adrenal gland of the frog, Rana ridibunda, was examined by the indirect immunofluorescence technique. Using an antiserum directed against rat alpha-CGRP, the presence of a network of positive fibers was observed in the adrenal pare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013380
更新日期:1994-07-01 00:00:00
abstract::Impaired regulation of hepatic glucose production is a characteristic feature of the metabolic syndrome, a cluster of diseases that includes obesity, insulin resistance, type 2 diabetes, and cardiovascular disease. It has been proposed that sustained endoplasmic reticulum stress, which appears to occur in obesity and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1014
更新日期:2006-01-01 00:00:00
abstract::Periodontitis, an inflammatory disease of periodontal tissues, is characterized by excessive alveolar bone resorption. An increase in the receptor activator of nuclear factor-κB ligand (RANKL) to osteoprotegerin (OPG) ratio is thought to reflect the severity of periodontitis. Here, we examined alveolar bone loss in OP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1928
更新日期:2013-02-01 00:00:00
abstract::GnRH neurons were harvested from adult male rats in order to determine if LH or FSH directly altered the modalities of GnRH release and/or cellular GnRH content. Cultures received vehicle, 25 ng/ml LH, 125 ng/ml LH, 25 ng/ml FSH, or 125 ng/ml FSH, and media samples were collected before treatment, during the treatment...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-1-200
更新日期:1987-07-01 00:00:00
abstract::Osteoclast precursors reach sites of osteoclast formation and remodelling via the vasculature and are therefore destined to encounter endothelium before migrating to the bone surface. Here we investigated the hypothesis that endothelium may be involved in the regulation of osteoclast precursor recruitment to sites of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.4.8204
更新日期:2001-04-01 00:00:00
abstract::The maternal skeleton resorbs during lactation to provide calcium to milk and the lost mineral content is restored after weaning. The changes are particularly marked in Ctcgrp null mice, which lose 50% of spine mineral content during lactation but restore it fully. The known calciotropic hormones are not required for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1931
更新日期:2013-04-01 00:00:00
abstract::In different types of mammalian cells, insulin has been shown to promote the release of an inositol phosphate glycan (InsP-glycan) through the hydrolysis of a glycosyl-phosphatidylinositol (glycosyl-PtdIns). This InsP-glycan, which has been demonstrated to be taken up by intact cells, may mediate some of the biologica...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119190
更新日期:1994-03-01 00:00:00
abstract::Morphological plasticity in response to estradiol is a hallmark of astrocytes in the arcuate nucleus. The functional consequences of these morphological changes have remained relatively unexplored. Here we report that in the arcuate nucleus estradiol significantly increased the protein levels of the two enzymes in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1701
更新日期:2009-07-01 00:00:00
abstract::Regulation of galanin gene expression in the anterior pituitary (AP) is positively influenced by estrogen in rodents and undetermined in humans. The objective of this study was to investigate the mechanism behind estrogen induction of galanin by identifying any putative estrogen receptor (ER) binding sequences within ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.11.5507
更新日期:1997-11-01 00:00:00
abstract::Follistatin (FS), which binds to the inhibin/activin beta A- or beta B-subunit is localized with and modulates the biological actions of activin in many systems. However, in contrast to the wide distribution of the activin beta-subunit proteins and messenger RNAs (mRNA) in the brain, demonstration of FS mRNA signal ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612560
更新日期:1996-05-01 00:00:00
abstract::Otsuka Long-Evans Tokushima fatty (OLETF) rats lacking cholecystokinin-A receptors are hyperphagic, obese, and diabetic. Although exercise attenuates OLETF rats' obesity, the mechanisms underlying the effects of exercise are unclear. In this study, we determined the effects of running wheel activity on patterns of bod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1441
更新日期:2005-04-01 00:00:00
abstract::The synthesis of androgens by theca-interstitial cells is stimulated by LH and the insulin/insulin-like growth factor I (IGF-I) system. An essential element of the steroidogenesis is the uptake of plasma cholesterol and transformation to steroid hormones. In the rat, the uptake of cholesterol by the theca-interstitial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7865
更新日期:2001-01-01 00:00:00
abstract::Xenoestrogens from synthetic or natural origin represent an increasing risk of disrupted endocrine functions including the physiological activity of the hypothalamo-pituitary-gonad axis. Ethinyl estradiol (EE2) is a synthetic estrogen used in contraceptive pills, whereas zearalenone (ZEA) is a natural mycoestrogen fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1330
更新日期:2015-11-01 00:00:00
abstract::Chronically elevated circulating glucocorticoid levels are although to enhance vulnerability to psychopathology. Here we hypothesized that such sustained glucocorticoid levels, disturbing corticosterone pulsatility, attenuate glucocorticoid receptor signaling and target gene responsiveness to an acute challenge in the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1119
更新日期:2010-03-01 00:00:00
abstract::A healthy nutritional state is required for all aspects of reproduction and is signaled by the adipokine leptin. Leptin acts in a relatively narrow concentration range: too much or too little will compromise fertility. The leptin signal timing is important to prepubertal development in both sexes. In the brain, leptin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa204
更新日期:2021-02-01 00:00:00
abstract::We have compared the physicochemical properties of rat uterine nuclear estrogen receptors (ER) labeled with (E2) or the high affinity antiestrogen 4-hydroxytamoxifen [OH Tam; 1-[4-(2-dimethylaminoethoxy)phenyl]-1-(4-hydroxyphenyl) 2-phenyl-but-1-(Z)ene]. Nuclear ER labeled in vivo with either ligand had sedimentation ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-904
更新日期:1986-08-01 00:00:00
abstract::The power of animal models is derived from the ability to control experimental variables so that observed effects may be unequivocally attributed to the factor that was changed. One variable that is difficult to control in animal experiments is the number and composition of offspring in a litter. To account for this v...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2016-1145
更新日期:2016-07-01 00:00:00
abstract::After 5 days of incubation with 10 nM tritiated dexamethasone, the amount of glucocorticoid displaceably-bound by the ACTH-secreting, glucocorticoid-responsive AtT-20 mouse pituitary tumor cell decreases by 70-80%. This decrease takes place in both the cytosolic and nuclear fractions. Although one might predict that t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-2-699
更新日期:1982-08-01 00:00:00
abstract::In the present study we examined the mechanisms involved in the activation of the hypothalamo-pituitary-adrenal (HPA) axis after administration of the glucose analog 2-deoxyglucose (2-DG), which inhibits intracellular glucose use. Adult male rats were injected with either 2-DG (400 mg/kg body wt ip) or vehicle and wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.4.8137760
更新日期:1994-04-01 00:00:00
abstract::To investigate the regulation of expression of the TSH receptor (TSHR) in extrathyroidal tissues, the level of TSHR messenger RNA (mRNA) and TSH-dependent signal transduction were determined in isolated rat adipocytes and cultured preadipocytes. The epididymal, sc, and perirenal, but not the interscapular brown adipos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754740
更新日期:1996-08-01 00:00:00
abstract::In adult rats hysterectomized on day 8 of pseudopregnancy, the mean serum progesterone (P) level fell from 88 ng/ml on day 8 to 44 ng/ml on day 15 (n = 61). In response to a single sc injection of 0.5 ml of a specific antiserum to LH (LHAS) on day 10, the P level fell to less than 10 by day 15 in 29 of 33 rats; howeve...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1750
更新日期:1986-10-01 00:00:00
abstract::The specific uptake by rat liver of human (hGH) and bovine (bGH) GHs labeled with 125I was studied by an in vivo procedure. A significant reduction of the uptake was observed when labeled hormones were injected together with different amounts of the corresponding native GH. This reduction was dose dependent, and the c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1190
更新日期:1978-10-01 00:00:00
abstract::The expression of the proopiomelanocortin (POMC) gene by normal lung and thyroid was examined by measurement of the content of ACTH, beta-lipotropin (beta LPH), and beta-endorphin (beta EP) in porcine lung and thyroid tissue. Acid extracts of normal porcine lung and thyroid tissue each contained appreciable amounts of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2097
更新日期:1982-12-01 00:00:00