Abstract:
:Xenoestrogens from synthetic or natural origin represent an increasing risk of disrupted endocrine functions including the physiological activity of the hypothalamo-pituitary-gonad axis. Ethinyl estradiol (EE2) is a synthetic estrogen used in contraceptive pills, whereas zearalenone (ZEA) is a natural mycoestrogen found with increasing prevalence in various cereal crops. Both EE2 and ZEA are agonists of estrogen receptor-α and accelerate puberty. However, the neuroendocrine mechanisms that are responsible for this effect remain unknown. Immature female Wistar rats were treated with EE2 (10 μg/kg), ZEA (10 mg/kg), or vehicle for 10 days starting from postnatal day 18. As a marker of puberty, the vaginal opening was recorded and neuropeptide and related transcription factor mRNA levels were measured by quantitative real time PCR and in situ hybridization histochemistry. Both ZEA and EE2 accelerated the vaginal opening, increased the uterine weight and the number of antral follicles in the ovary, and resulted in the increased central expression of gnrh. These changes occurred in parallel with an earlier increase of kiss1 mRNA in the anteroventral and rostral periventricular hypothalamus and an increased kisspeptin (KP) fiber density and KP-GnRH appositions in the preoptic area. These changes are compatible with a mechanism in which xenoestrogens overstimulate the developmentally unprepared reproductive system, which results in an advanced vaginal opening and an enlargement of the uterus at the periphery. Within the hypothalamus, ZEA and EE2 directly activate anteroventral and periventricular KP neurons to stimulate GnRH mRNA. However, GnRH and gonadotropin release and ovulation are disrupted due to xenoestrogen-mediated inhibitory KP signaling in the arcuate nucleus.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Kriszt R,Winkler Z,Polyák Á,Kuti D,Molnár C,Hrabovszky E,Kalló I,Szőke Z,Ferenczi S,Kovács KJdoi
10.1210/en.2015-1330subject
Has Abstractpub_date
2015-11-01 00:00:00pages
3996-4007issue
11eissn
0013-7227issn
1945-7170journal_volume
156pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::MRL-lpr/lpr mice manifest a systemic lupus-like autoimmune disease. As part of this syndrome, the mice spontaneously develop autoimmune thyroiditis, which is morphologically and biochemically similar to human autoimmune thyroiditis. In this study we investigated whether thyroid tissue obtained from sites of chronic in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628400
更新日期:1995-08-01 00:00:00
abstract::We previously reported a cooperative effect between insulin-like growth factor I (IGF-I) and 1,25-dihydroxy-vitamin D3 [1,25-(OH)2D3] in murine clonal osteoblastic cells, MCT3T3-E1. In the present study, the possible mechanism of interaction between these hormones was investigated. The effect of IGF-I on 1,25-(OH)2D3 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-4-2088
更新日期:1990-04-01 00:00:00
abstract::Oxytocin (OT) is a hypothalamic nonapeptide that is synthesized as part of a larger precursor protein that also contains an approximately 10-kDa protein called neurophysin at its C-terminus. This precursor protein is trafficked through the regulated secretory pathway into secretory granules and then axonally transport...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8700
更新日期:2002-03-01 00:00:00
abstract::We present a map describing the antigenic determinants (epitopes) of the human CG (hCG) molecule. A panel of monoclonal antibodies to hCG, previously characterized by us was incorporated into two-site binding assays to probe hCG by a 21 X 11 matrix of 231 pairs of MCA. Nine different reaction patterns of reciprocal ho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-189
更新日期:1986-01-01 00:00:00
abstract::Benign prostatic hyperplasia (BPH) is an age-related debilitating prostatic disease that is frequently associated with prostatic inflammation and bothersome lower urinary tract symptoms (LUTS). Animal models have shown that formalin- and bacterial-induced prostatic inflammation can induce bladder dysfunction; however,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa212
更新日期:2021-01-01 00:00:00
abstract::Growth hormone (GH) is the key hormone involved in the regulation of growth and metabolism, two functions that are highly modulated during infancy. GH secretion, controlled mainly by GH releasing hormone (GHRH), has a characteristic pattern during postnatal development that results in peaks of blood concentration at b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0298
更新日期:2010-12-01 00:00:00
abstract::Desensitization and internalization of G protein-coupled receptors can be mediated by phosphorylation within the C-terminal tail, facilitating beta-arrestin binding and targeting the receptor for internalization. Type II GnRH receptors (GnRH-Rs) show such regulation, but type I GnRH-Rs lack C-tails and are not rapidly...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0092
更新日期:2004-08-01 00:00:00
abstract::Endocrine regulation of uterine biology is critical for embryo receptivity and human reproduction. Uterine endometrium depends on extrinsic sex steroid input and hence likely has mechanisms that enable adaptation to hormonal variation. Emerging evidence suggests that sex steroid bioavailability in the endometrium is d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1139
更新日期:2014-11-01 00:00:00
abstract::The majority of ovarian follicles undergo atresia through a mechanism involving apoptotic cell death. Although GnRH and its agonists have been shown to suppress ovarian growth and differentiation in hypophysectomized rats, studies on the induction of follicle atresia by GnRH are contradictory. In the present study, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275940
更新日期:1994-01-01 00:00:00
abstract::Tear fluid is known to contain many different hormones with relevance for ocular surface homeostasis. We studied the presence and functional role of insulin-like factor 3 (INSL3) and its cognate receptor RXFP2 (relaxin/insulin-like family peptide receptor 2) at the ocular surface and in tears. Expression of human INSL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2201
更新日期:2013-06-01 00:00:00
abstract::It has been suggested that ewes carrying the Booroola gene (Fec(B)) consistently ovulate more follicles because they recruit more primordial follicles and/or have a lower rate of atresia. If the former is correct, the pool of follicles would be depleted sooner in Fec(B) animals. We have studied follicular dynamics and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1722
更新日期:2004-06-01 00:00:00
abstract::Androgens are the primary regulators of epididymal structure and functions. Principal cells are the major cell type of the tissue and are particularly sensitive to androgen removal. To distinguish the direct effects of androgens on this cell type, DNA microarrays were used to identify androgen-regulated genes in the m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0986
更新日期:2007-01-01 00:00:00
abstract::Hypothalamic proopiomelanocortin (POMC)-derived MSH peptides and the melanocortin receptor antagonist, agouti-related protein (AgRP), interact to regulate energy balance. Both POMC and AgRP neurons express estrogen receptors, but little is known about estrogen regulation of the melanocortin system in the primate. We h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0853
更新日期:2010-03-01 00:00:00
abstract::There is increasing evidence that protein kinase C (PKC) isoforms modulate insulin-signaling pathways in both positive and negative ways. Recent reports have indicated that the novel PKCdelta mediates some of insulin's actions in muscle and liver cells. Many studies use the specific inhibitor rottlerin to demonstrate ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220259
更新日期:2002-10-01 00:00:00
abstract::The Leydig cells of the testis are known to possess high affinity receptors for luteinizing hormone and human chorionic gonadotropin (hCG), but no information concerning the synthesis of these receptors is available yet. In order to investigate this question, we have purified crude rat interstitial cell preparations o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-2129
更新日期:1982-12-01 00:00:00
abstract::The induction of long-term responses in the uterus following estrogen treatment is discussed, with special reference to DNA synthesis. Immature female rats injected daily with estradiol-17beta or estriol (0.01 to 1 mug) or a combination of the two steroids for one, two or three consecutive days were sacrificed at inte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1501
更新日期:1976-12-01 00:00:00
abstract::CRF activity of synthetic vasopressins and pitressin was studied in an in vitro system of cultured rat adenohypophyseal cells using direct measurement of ACTH by radioimmunoassay. Pitressin (posterior pituitary extract) induced a dose-related secretion of ACTH whereas synthetic arginine or lysine vasopressin were devo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-4-936
更新日期:1976-04-01 00:00:00
abstract::A cell-free system was used to characterize the binding reaction between the progesterone receptor and nuclear acceptor sites prepared from rat placenta. Two forms of receptor-acceptor complex were examined. One was extracted from nuclei by exposure to 0.6 M KCl; the other type was resistant to salt extraction. Kineti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-1-28
更新日期:1987-07-01 00:00:00
abstract::Prolactin has been associated with many effects and has been implicated in the pathogenesis of pregnancy-related hypertensive disorders, although little is known about its vascular effects. The present study was designed to determine the primary effect of prolactin on regional vascular beds and the mechanisms involved...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1577
更新日期:2007-08-01 00:00:00
abstract::Levels of bovine placental lactogen (bPL) have been measured in the serum of dairy and beef cattle and in the milk and amniotic fluid of pregnant animals with a highly specific radioimmunoassay. In both dairy and beef cows, serum bPL levels remain low (less than 50 ng/ml) during the first two trimesters and then rise ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-5-1273
更新日期:1976-11-01 00:00:00
abstract::The Kiss1 gene and its product kisspeptin are important regulators of reproduction. In rodents, Kiss1 is expressed in the hypothalamic arcuate (ARC) and anteroventral periventricular (AVPV)/rostral periventricular (PeN) nuclei. In the AVPV/PeN, females have more Kiss1 and tyrosine hydroxylase (TH) neurons than males. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0783
更新日期:2010-12-01 00:00:00
abstract::The ontogeny of expression of the CRF gene in the paraventricular hypothalamic nucleus and POMC gene in the pituitary was studied in rats using in situ hybridization histochemistry and Northern blotting techniques, respectively. CRF mRNA was first detected on day 17 of gestation (E17) in the paraventricular nucleus of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-60
更新日期:1989-01-01 00:00:00
abstract::In the developing follicle, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are the primary stimulators of steroidogenesis in granulosa and theca cells. The steroidogenic actions of both these gonadotropins are largely if not exclusively mediated through cAMP. Previous studies have shown that EGF and T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-5-2790
更新日期:1991-11-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed at a high level in the fetal pituitary and decreases profoundly between embryonic day 19 and postnatal day 1 (PN1), with a further decrease from PN1 to PN4. In this series of experiments, we investigated the hypothesis that dopamine 2 receptor (Dr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2147
更新日期:2014-07-01 00:00:00
abstract::In the pituitary, GABA regulates the release of several hormones via different receptors. GABA(C) receptors are heterooligomers that differ from GABA(A) receptors in that they contain p-subunits and are insensitive to bicuculline. However, molecular and functional evidence for the presence of GABA(C) receptors outside...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.5.7476
更新日期:2000-05-01 00:00:00
abstract::Administration of dehydroepiandrosterone (DHA) to immature female rats on day 27 for 3 days resulted in an increase in uterine weight within 6 h of injection and a surge of FSH, LH and prolactin occurred on day 30 resulting in premature ovulation. Increase in ovarian weight and vaginal patency also occurred on day 30....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-458
更新日期:1975-08-01 00:00:00
abstract::In young adult female rats, the patterns of inhibin subunit mRNA expression during the estrous cycle are regulated by cyclic changes in gonadotropin secretion and follicular development. Since there are distinct alterations in profiles of both hormone secretion and folliculogenesis during the reproductive lifespan of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.1.8419130
更新日期:1993-01-01 00:00:00
abstract::Transfer of corticosterone from serum to milk was studied in lactating rats after ether and pentobarbital anesthesia. Two minutes after the onset of anesthesia stress, total serum and milk corticosterone concentrations were not significantly different. By 30 min, corticosterone concentrations in serum increased to a p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1364
更新日期:1984-10-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) comprise one of the largest subgroups in the TGF-β ligand superfamily. We have identified a functional BMP system equipped with the ligand (BMP4), receptors (BMP type II receptor, BMP type IA receptor, also called ALK3) and the signaling proteins, namely the mothers against decapenta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1942
更新日期:2015-07-01 00:00:00
abstract::We recently reported that long-term treatment with pioglitazone restored cardiac Akt phosphorylation in response to hyperthermia (HT) and subsequent cardiac heat-shock protein 72 (HSP72) expression, in heredity insulin resistance rats via improvement of insulin sensitivity. Because adrenomedullin (AM) promotes Akt pho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1052
更新日期:2009-03-01 00:00:00