Abstract:
:Endometriosis-associated infertility has a multifactorial etiology. We tested the hypothesis that the endometrial response to the early embryonic signal, human chorionic gonadotropin (hCG), alters over time in a nonhuman primate model of endometriosis. Animals with experimental or spontaneous endometriosis were treated with hCG (30 IU/d), from d 6 after ovulation for 5 d, via an oviductal cannula. Microarray analysis of endometrial transcripts from baboons treated with hCG at 3 and 6 months of disease (n=6) identified 22 and 165 genes, respectively, whose levels differed more than 2-fold compared with disease-free (DF) animals treated with hCG (P<0.01). Quantitative RT-PCR confirmed abnormal responses of known hCG-regulated genes. APOA1, SFRP4, and PAPPA, which are normally down-regulated by hCG were up-regulated by hCG in animals with endometriosis. In contrast, the ability of hCG to induce SERPINA3 was lost. Immunohistochemistry demonstrated dysregulation of C3 and superoxide dismutase 2 proteins. We demonstrate that this abnormal response to hCG persists for up to 15 months after disease induction and that the nature of the abnormal response changes as the disease progresses. Immunohistochemistry showed that this aberrant gene expression was not a consequence of altered LH/choriogonadotropin receptor distribution in the endometrium of animals with endometriosis. We have shown that endometriosis induces complex changes in the response of eutopic endometrium to hCG, which may prevent the acquisition of the full endometrial molecular repertoire necessary for decidualization and tolerance of the fetal allograft. This may in part explain endometriosis-associated implantation failure.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Sherwin JR,Hastings JM,Jackson KS,Mavrogianis PA,Sharkey AM,Fazleabas ATdoi
10.1210/en.2010-0275subject
Has Abstractpub_date
2010-10-01 00:00:00pages
4982-93issue
10eissn
0013-7227issn
1945-7170pii
en.2010-0275journal_volume
151pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Agouti-related protein (AGRP) is a homologue of the agouti gene product and, when overexpressed, promotes obesity. Like neuropeptide Y (NPY) messenger RNA (mRNA), AGRP mRNA is produced in the hypothalamic arcuate nucleus and is elevated in leptin-deficient ob/ob and leptin-resistant db/db mice. These data suggest that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6491
更新日期:1999-02-01 00:00:00
abstract::A variety of transcription factors including Wilms tumor gene (Wt-1), steroidogenic factor 1 (Sf-1), dosage-sensitive sex reversal, adrenal hypoplasia congenita on the X-chromosome, Gene 1 (Dax-1), and pre-B-cell transcription factor 1 (Pbx1) have been defined as necessary for regular adrenocortical development. Howev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0681
更新日期:2007-02-01 00:00:00
abstract::Patients with Graves' disease and exophthalmos demonstrate delayed hypersensitivity to antigens present in extracts of certain normal human tissue; namely,thyroid gland and retroorbital tissue. The delayed hypersensitivity can be assayed in vitro by quantitating the amount of a lymphokine, migration inhibition factor ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-351
更新日期:1977-02-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta) is a multifunctional growth factor in bone that is secreted as a latent complex and must be activated in order to influence cellular activity. We have investigated the influence of dexamethasone (Dex; a potent glucocorticoid) on the secretion and activation of latent TGF beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404670
更新日期:1993-11-01 00:00:00
abstract::In vitro addition of rat insulin (200, 400 or 800 muU/ml) to collagenase-isolated pancreatic islets of adult rats diminished glucose (3 mg/ml)-induced insulin release which was correlated with a decrease of the ratio of total NADPH/NADP and inhibition of glucose oxidation via the pentose phosphate shunt (PPS). NADH an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-6-1469
更新日期:1976-12-01 00:00:00
abstract::The GnRH decapeptide controls reproductive function through its release from neuroendocrine terminals in the median eminence, a site where there is a convergence of numerous nerve terminals and glial cells. Previous work showed dynamic changes in the GnRH-glial-capillary network in the median eminence under different ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0679
更新日期:2009-12-01 00:00:00
abstract::Reproductive function is dependent on the interaction between GnRH and its cognate receptor found on gonadotrope cells of the anterior pituitary gland. GnRH activation of the GnRH receptor (GnRHR) is a potent stimulus for increased expression of multiple genes including the gene encoding the GnRHR itself. Thus, homolo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220784
更新日期:2003-03-01 00:00:00
abstract::Acute effects of bisphenol (BPA), an environmental chemical, on estradiol (17α or β-E2)-dependent recognition memory and dendritic spines in the medial prefrontal cortex and hippocampus were investigated in adult female rats. Ovariectomized rats received BPA 30 min before or immediately after a sample trial (viewing o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1121
更新日期:2012-07-01 00:00:00
abstract::The active form of vitamin D, 1,25-dihydroxyvitamin D3, [1,25(OH)2D3] has potent actions on innate and adaptive immunity. Although endocrine synthesis of 1,25(OH)2D3 takes place in the kidney, the enzyme that catalyzes this, 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27b1 in humans, Cyp27b1 in mice), is expressed at m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0060
更新日期:2008-10-01 00:00:00
abstract::The physiology and regulation of steroid synthesis in the brain have emerged as important for understanding brain function. Neurosteroids, those steroids synthesized de novo in nervous tissue, have been associated with numerous central nervous system functions, including myelination, mental retardation, and epilepsy. ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2008-0011
更新日期:2008-06-01 00:00:00
abstract::We have demonstrated TSH binding sites in membranes prepared from guinea pig testis. The binding of TSH to testicular homogenate was localized to the interstitial tissue and showed similar characteristics to the binding of TSH to thyroid membranes with high affinity (Ka = 2.5 X 10(10) M-1) but limited capacity (approx...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-1-6
更新日期:1978-07-01 00:00:00
abstract::We compared the somatomedin (SM) activity of perfusates of livers from normal and hypophysectomized (hypox) rats with and without the addition of growth hormone (GH) to the perfusions. Livers were perfused for a total of 3 hours, with a change of Waymouth's medium MB 752/l every 30 min, in a continously recirculating ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-3-606
更新日期:1976-03-01 00:00:00
abstract::As part of an ongoing investigation of the role of metabolic activation of estrogens in the genesis of cancers such as estrogen-induced renal tumors in hamsters, we have 1) determined steroid-17 beta-oxidoreductase activity of microsomes and cytosol prepared from hamster kidney and liver; 2) compared the rates of 2-, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.5.7956900
更新日期:1994-11-01 00:00:00
abstract::The population of LHRH neurons was examined in young and middle-aged female rats at two different time points associated with the LH surge on proestrus. More immunopositive LHRH neurons were detected in the brains of young but not middle-aged females killed on the evening of proestrus (1830-2030 h) compared with those...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.8275960
更新日期:1994-01-01 00:00:00
abstract::Circulating levels of calcitonin precursors (CTpr), including procalcitonin (ProCT), increase up to several thousand-fold in human sepsis, and immunoneutralization improves survival in two animal models of this disease. Herein, we analyzed inflammation-mediated calcitonin I gene (CALC I) expression in human adipocyte ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0854
更新日期:2003-12-01 00:00:00
abstract::The effects of epidermal growth factor (EGF) on glycogen metabolism and the binding of [125I]iodo-EGF to receptors in fetal rat hepatocytes have been examined. The actions of EGF have been compared with those of insulin-like growth factor I (IGF-I) and insulin. EGF (0.1-45 nM) stimulated dose-dependent increases in [1...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-522
更新日期:1986-08-01 00:00:00
abstract::In male hamsters, light deprivation was reported to reduce both testis weight and prolactin (PRL) levels. Therefore, we decided to examine the effects of PRL on testicular function in hamsters exposed to short day-length. Adult hamsters were exposed to 5 h of light per day to induce gonadal atrophy, and, starting two ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:
更新日期:1975-12-01 00:00:00
abstract::Prolonged exposure to elevated glucocorticoid levels is known to produce insulin resistance (IR), a hallmark of diabetes mellitus. Although not fully elucidated, the underlying molecular mechanisms by which glucocorticoids induce IR may provide potential targets for pharmacological interventions. Here we characterized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1214
更新日期:2008-02-01 00:00:00
abstract::The human estrogen receptor alpha (ERalpha) and the recently identified ERbeta share a high degree of amino acid homology; however, there are significant differences in regions of these receptors that would be expected to influence transcriptional activity. Consequently, we compared the mechanism(s) by which these rec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.12.7179
更新日期:1999-12-01 00:00:00
abstract::A thyroid transcription factor-1 (TTF-1)-binding element in the rat TSH receptor (TSHR) promoter, between -189 and -175 basepairs (bp), is important for both thyroid-specific expression and thyroid-specific TSH/cAMP autoregulation of the TSHR. The identification of an up-stream TTF-1-binding site and its relationship ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828540
更新日期:1995-01-01 00:00:00
abstract::To determine if oxytocin (OT) is present in cynomolgus monkey corpus luteum, OT was measured by a specific and sensitive RIA in 13 corpora lutea, ovarian venous plasma on the ipsilateral side and peripheral venous plasma at different stages of the luteal phase. Serial dilution of acetic acid extract of the corpus lute...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-570
更新日期:1984-08-01 00:00:00
abstract::The aim of this study was to determine whether bisphenol A (BPA) has adverse effects on cardiovascular functions in CD-1 mice and define sex-specific modes of BPA action in the heart. Dams and analyzed progeny were maintained on a defined diet containing BPA (0.03, 0.3, 3, 30, or 300 ppm) that resulted in BPA exposure...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1847
更新日期:2015-03-01 00:00:00
abstract::Various neuropeptides have recently been isolated from the thymus, suggesting an interaction between the neuroendocrine and immune systems. We report here that the human thymus synthesizes a further peptide hormone, namely atrial natriuretic peptide (ANP). This is documented by the following findings. ANP and its proh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2277
更新日期:1990-05-01 00:00:00
abstract::PRL receptor (PRL-R) expression has been analyzed in human hematopoietic tissues using flow cytofluorometric analysis with a series of biotinylated monoclonal antibodies (mAbs) directed against the extracellular domain of the rat liver PRL-R. In the thymus, more than 75% of cells were labeled by the anti-PRL-R mAb. Re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.5.8156910
更新日期:1994-05-01 00:00:00
abstract::Regularly menstruating female rhesus monkeys were implanted sc with Silastic tubing packed with estrone. The implants were replaced every 6-8 months to maintain the serum estrone and estradiol concentrations constantly elevated 1.5-2.5-fold, i.e. in the midfollicular phase range, for 4 yr. Increasing the estrone and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2373
更新日期:1989-05-01 00:00:00
abstract::T(4) activation into T(3) is catalyzed by type 2 deiodinase (D2) in the brain. The rapid induction of D2 in astrocytes by transient brain ischemia has prompted us to explore the effects of hypoxia on D2 in cultures of astrocytes. Hypoxia (2.5% O(2)) of cultured astrocytes increased D2 activity, alone or in association...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0625
更新日期:2007-10-01 00:00:00
abstract::Periodontitis, an inflammatory disease of periodontal tissues, is characterized by excessive alveolar bone resorption. An increase in the receptor activator of nuclear factor-κB ligand (RANKL) to osteoprotegerin (OPG) ratio is thought to reflect the severity of periodontitis. Here, we examined alveolar bone loss in OP...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1928
更新日期:2013-02-01 00:00:00
abstract::Ovarian hormones, including 17β-estradiol, are implicated in numerous physiological processes, including sleep. Beginning at puberty, girls report more sleep complaints than boys, which is maintained throughout the reproductive life stage. Sleep problems are exacerbated during the menopausal transition, evidenced by g...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa128
更新日期:2020-09-01 00:00:00
abstract::Steroid hormone synthesis is a vital function of the adrenal cortex, serves a critical role in gonadal function, and maintains pregnancy if normally executed in the placenta. The substrate for the synthesis of all steroid hormones is cholesterol, and its conversion to the first steroid, pregnenolone, by the cholestero...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0541
更新日期:2009-02-01 00:00:00
abstract::Sodium vanadate, an agent known to have multiple cellular actions, was studied for its effects on aspects of bone formation in cultures of 21-day-old fetal rat calvariae. Vanadate (0.1-10 microM) stimulated the incorporation of [3H] thymidine into acid-insoluble residues (DNA); the effect appeared after 3 h and was su...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-855
更新日期:1985-03-01 00:00:00