Abstract:
:Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. However, mechanisms by which VDR regulates muscle function and morphology remain unclear. A crucial question is whether VDR is expressed in skeletal muscle and directly alters muscle physiology. Using PCR, Western blotting, and immunohistochemistry (VDR-D6 antibody), we detected VDR in murine quadriceps muscle. Detection by Western blotting was dependent on the use of hyperosmolar lysis buffer. Levels of VDR in muscle were low compared with duodenum and dropped progressively with age. Two in vitro models, C2C12 and primary myotubes, displayed dose- and time-dependent increases in expression of both VDR and its target gene CYP24A1 after 1,25(OH)2D (1,25 dihydroxyvitamin D) treatment. Primary myotubes also expressed functional CYP27B1 as demonstrated by luciferase reporter studies, supporting an autoregulatory vitamin D-endocrine system in muscle. Myofibers isolated from mice retained tritiated 25-hydroxyvitamin D3, and this increased after 3 hours of pretreatment with 1,25(OH)2D (0.1 nM). No such response was seen in myofibers from VDR knockout mice. In summary, VDR is expressed in skeletal muscle, and vitamin D regulates gene expression and modulates ligand-dependent uptake of 25-hydroxyvitamin D3 in primary myofibers.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Girgis CM,Mokbel N,Cha KM,Houweling PJ,Abboud M,Fraser DR,Mason RS,Clifton-Bligh RJ,Gunton JEdoi
10.1210/en.2014-1016subject
Has Abstractpub_date
2014-09-01 00:00:00pages
3227-37issue
9eissn
0013-7227issn
1945-7170journal_volume
155pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::A study of the synthesis and migration of thyroglobulin has been carried out by the incubation of calf thyroid slices in the presence of [14C]leucine followed by the separation of intracellular compartments from the colloid by differential and density gradient centrifugation. The effect of inhibitors on these processe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-3-1146
更新日期:1986-09-01 00:00:00
abstract::Alzheimer's disease (AD) is a progressive neurodegenerative disorder whose etiology is presently unknown. Probably the most consistent and widespread deficit seen in this syndrome is that of the basal forebrain cholinergic system. We have previously demonstrated that estradiol (E2) modulates the function of these neur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720680
更新日期:1995-05-01 00:00:00
abstract::Synthetic selective thyroid hormone (TH) receptor (TR) modulators (STRM) exhibit beneficial effects on dyslipidemias in animals and humans and reduce obesity, fatty liver, and insulin resistance in preclinical animal models. STRM differ from native TH in preferential binding to the TRβ subtype vs. TRα, increased uptak...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1325
更新日期:2012-01-01 00:00:00
abstract::The scaffolding protein, protein targeting to glycogen (PTG), orchestrates the signaling of several metabolic enzymes involved in glycogen synthesis. However, little is known concerning the regulation of PTG itself. In this study, we have cloned and characterized the mouse promoter of PTG. We identified multiple FoxA2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1513
更新日期:2006-07-01 00:00:00
abstract::Morphine sulfate was found to have a direct inhibitory effect on both basal and GnRH-stimulated LH release by cultured rat pituitary cells. The inhibitory effect of morphine on LH release was prevented by the opiate antagonist naltrexone, and treatment of cells with naltrexone or beta-endorphin antiserum significantly...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-5-2097
更新日期:1986-05-01 00:00:00
abstract::Type 3 deiodinase (D3), the physiologic inactivator of thyroid hormones, is induced during tissue injury and regeneration. This has led to the hypotheses that D3 impacts injury tolerance by reducing local T3 signaling and contributes to the fall in serum triiodothyronine (T3) observed in up to 75% of sick patients (te...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2028
更新日期:2014-10-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta) is a multifunctional growth factor in bone that is secreted as a latent complex and must be activated in order to influence cellular activity. We have investigated the influence of dexamethasone (Dex; a potent glucocorticoid) on the secretion and activation of latent TGF beta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404670
更新日期:1993-11-01 00:00:00
abstract::Altered leptin action has been implicated in the pathophysiology of heart failure in obesity, a hallmark of which is extracellular matrix remodeling. Here, we characterize the direct influence of leptin on matrix metalloproteinase (MMP) activity in primary adult rat cardiac fibroblasts and focus on elucidating the mol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1166
更新日期:2011-05-01 00:00:00
abstract::Various neuropeptides have recently been isolated from the thymus, suggesting an interaction between the neuroendocrine and immune systems. We report here that the human thymus synthesizes a further peptide hormone, namely atrial natriuretic peptide (ANP). This is documented by the following findings. ANP and its proh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2277
更新日期:1990-05-01 00:00:00
abstract::The effects of endothelin-1 (ET-1), a potent vasoconstrictor and mitogen to various cell types, on proliferation and differentiated functions of the murine Leydig tumor cell line MA-10 were investigated. Kinetic binding experiments at room temperature showed that [125I] ET-1 bound to MA-10 cells and reached equilibriu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425480
更新日期:1993-02-01 00:00:00
abstract::Primary kidney cell cultures from normal rhesus monkeys were used to study the regulation of 24,25-dihydroxyvitamin D3 production. Kidney cells were grown to confluency in the modified National Cancer Institute Medium NCTC 135 with 2% fetal calf serum and then maintained in a serum-free medium (2% bovine serum albumin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-4-1184
更新日期:1977-10-01 00:00:00
abstract::Recent evidence suggests that the aromatization of testosterone to estrogen is important for the organizing effects of neonatal testosterone on neuroendocrine responses to acute challenges. However, the extent to which neonatal inhibition of aromatase alters the stress-induced activation of neural pathways has not bee...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1749
更新日期:2012-04-01 00:00:00
abstract::GnRH neurons form the final common pathway regulating the secretion of gonadotropins from the anterior pituitary. Since the patterns of gonadotropin release display profound sexual dimorphism among mammals including the rodent, we undertook an ultrastructural analysis to determine whether these neurosecretory cells we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-695
更新日期:1990-02-01 00:00:00
abstract::To analyze the molecular origin of an ER variant, the truncated ER product-1, transiently expressed at the proestrus in lactotrope cells, we generated a 2.5-kb sequence of a genomic region upstream and downstream the specific sequence truncated ER product-1. Genomic Southern blot analysis showed that truncated ER prod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8392
更新日期:2001-09-01 00:00:00
abstract::Recent studies suggest that GH may modulate emotion, behavior, or stress response by its direct actions on the brain, and possible expression of the GH gene in the brain has been predicted. In this study we have investigated whether and where the GH gene is expressed in the brain and how it is regulated. Ribonuclease ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6009
更新日期:1998-05-01 00:00:00
abstract::Galanin-like peptide (GALP), a 29-amino-acid neuropeptide, is located in the hypothalamic arcuate nucleus (ARC), binds to galanin receptor subtype 2, and induces food intake upon intracerebroventricular (icv) injection in rats. However, neural mechanisms underlying its orexigenic action remain unclear. We aimed to ide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0907
更新日期:2006-04-01 00:00:00
abstract::Sodium butyrate (NaB), a dietary micronutrient, is a potent growth inhibitor that initiates cell differentiation in many cell types, including prostate cancer cells. The molecular mechanisms by which these effects occur remain largely unknown. In this study, we investigated the effects of NaB on the expression of IGF ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8766
更新日期:2002-05-01 00:00:00
abstract::Dexamethasone, a synthetic glucocorticoid, inhibits the initial rate of transport of the nonmetabolizable amino acid, alpha-aminoisobutyric acid, in rat hepatoma tissue culture (HTC) cells. To determine whether this inhibition is mediated by the same proximal steps as is the steroidal induction of tyrosine aminotransf...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-476
更新日期:1981-08-01 00:00:00
abstract::Proteolytic degradation of extracellular matrix components has been suggested to play an essential role in the occurrence of ovulation. Recent studies in our laboratory have indicated that the plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are no...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1736
更新日期:2008-10-01 00:00:00
abstract::In previous work we showed that the chondrodysplastic phenotype of mice homozygous for a null mutation of the PTH-related peptide (PTHrP) gene was due in part to reduced proliferation and aberrant differentiation of growth plate chondrocytes. In the present study we have extended those observations by examining chondr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895380
更新日期:1996-11-01 00:00:00
abstract::GRK2 is a member of the G protein-coupled receptor kinase family that phosphorylates the activated form of beta-adrenergic and other G protein-coupled receptors and plays an important role in their desensitization and modulation. Alterations in thyroid hormone levels have been reported to lead to important changes in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8039
更新日期:2001-03-01 00:00:00
abstract::Genetically obese Zucker rats, like obese humans, have normal or elevated circulating insulin-like growth factor-I (IGF-I) levels in the presence of low GH secretion. Hyperinsulinemia, increased energy status, or other nutritional factors associated with obesity could be responsible for these findings directly by incr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4975
更新日期:1997-03-01 00:00:00
abstract::Both somatostatin (SRIF) and urotensin II, a dodecapeptide from the teleost caudal neurosecretory system, inhibit PRL release from the organ-cultured rostral pars distalis of the tilapia, Sarotherodon mossambicus, in a dose-related manner. The inhibitory action of SRIF on PRL release was completely prevented by the pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-3-910
更新日期:1982-03-01 00:00:00
abstract::Actions of protein products resulting from alternative splicing of the Igf1 gene have received increasing attention in recent years. However, the significance and functional relevance of these observations remain poorly defined. To address functions of IGF-I splice variants, we examined the impact of loss of IGF-IEa a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1279
更新日期:2011-05-01 00:00:00
abstract::Similar to insulin, osmotic shock treatment of 3T3-L1 adipocytes causes translocation of GLUT4 protein to the plasma membrane and an increase in glucose transport activity. In our study, we evaluated the effect of chronic insulin treatment on the osmotic shock signaling pathway leading to GLUT4 translocation and gluco...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7801
更新日期:2000-12-01 00:00:00
abstract::Potassium deficiency produced by feeding mice a low potassium diet caused a marked decrease in plasma and testicular testosterone concentrations and a concomitant fall in the weight of seminal vesicles and in renal ornithine decarboxylase activity. All of these parameters were rapidly restored when potassium supply wa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.9.8756540
更新日期:1996-09-01 00:00:00
abstract::The rate of internalization and degradation of radioiodinated hCG and ovine LH (oLH) as well as homologous and heterologous recombinants of their subunits was studied in suspensions of ovine luteal cells. Hormone bound to the plasma membrane was quantified by treating the cells with acidic buffer (pH 3.0) and quantifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-265
更新日期:1983-07-01 00:00:00
abstract::The Src homology 2-containing tyrosine phosphatase, Shp-2, is a crucial enzyme that mediates intracellular signaling and is implicated in cell proliferation and differentiation. Here we investigated the involvement of the Shp-2 tyrosine phosphatase in determining the downstream signaling pathways initiated by the Ret ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0620
更新日期:2003-10-01 00:00:00
abstract::The two phases of the ovulatory menstrual cycle of the primate are characterized by divergent activities of the GnRH pulse generator. During the luteal phase, LH pulse frequency is significantly reduced below that observed during the follicular phase. In this report we investigate whether the decrease in pulse frequen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-2044
更新日期:1987-05-01 00:00:00
abstract::Progestins have been implicated in breast cancer development, yet a role for progesterone (Pg) in ductal morphogenesis (DM) has not been established. To determine whether Pg could cause DM, we compared relative effects of Pg, estradiol (E2) and IGF-I on anatomical and molecular biological parameters of IGF-I-related D...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1360
更新日期:2005-03-01 00:00:00