Abstract:
:The rate of internalization and degradation of radioiodinated hCG and ovine LH (oLH) as well as homologous and heterologous recombinants of their subunits was studied in suspensions of ovine luteal cells. Hormone bound to the plasma membrane was quantified by treating the cells with acidic buffer (pH 3.0) and quantification of the radioactivity that was removed from receptor. The radioactivity remaining in the cell pellet was considered to be intracellular. The extent of degradation of the radioactive hormones was determined by subjecting the radioactivity released into the medium during incubation to precipitation with 20% trichloroacetic acid. The non-precipitable radioactivity was considered to have been degraded. Radioactivity was lost from the cell membrane with a t1/2 of 16.8 +/- 2.5 h for radioiodinated hCG, 22.8 +/- 3.8 h for the alpha-subunit of hCG recombined with radioiodinated beta-subunit of hCG, 8.9 +/- 4.5 h for the alpha-subunit of oLH recombined with the radioiodinated beta-subunit of hCG, 0.5 +/- 0.1 h for the alpha-subunit of hCG recombined with the radioiodinated beta-subunit of oLH, 0.5 +/- 0.1 h for radioiodinated oLH, and 0.7 +/- 0.2 h for the alpha-subunit of oLH recombined with the radioiodinated beta-subunit of oLH. In general, the levels of radioactivity that were intracellular and the rate of degradation of the hormone were inversely related to the quantities of hormone that remained on the plasma membrane. The preparations that contained the beta-subunit of hCG were all internalized at a slower rate (t1/2 = 9-22 h) and consequently were degraded more slowly than any of the preparations that contained the beta-subunit of oLH (t1/2 = 0.5-0.7 h). When the radioiodine in oLH was present in the beta-subunit, intracellular levels of radioactivity were higher and degradation occurred more rapidly than when the radioiodine was in the alpha-subunit. Although the differences were less dramatic, hCG with radioiodine in the beta-subunit also reached higher levels intracellularly, but was degraded to a lesser extent at 16 and 24 h than was hCG with radioiodine in the alpha-subunit. These data demonstrated a dramatic difference (approximately 30-fold) in the rate of loss of oLH vs. that of hCG from the membrane of ovine luteal cells. Further, it appears that the beta-subunit of each of the two hormones is the major factor in determining the rate of internalization of the intact hormone. The differences is how the two hormones are processed at the receptor level may help explain the known differences in their steroidogenic potencies.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Mock EJ,Papkoff H,Niswender GDdoi
10.1210/endo-113-1-265subject
Has Abstractpub_date
1983-07-01 00:00:00pages
265-9issue
1eissn
0013-7227issn
1945-7170journal_volume
113pub_type
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