Abstract:
:PTH stimulates synthesis and secretion of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in renal proximal tubule cells through activation of the protein kinase-A (PKA) or the protein kinase-C (PKC) signaling pathway. The relative contribution of the two transducing systems was explored using PTH fragments with selective activation of either PKA or PKC. Rat renal proximal tubules were isolated by Percoll centrifugation, and PKA and PKC activities were measured after treatment with synthetic fragments and analogs of PTH. Rat PTH-(1-34), [Nle8,Nle15,Tyr34]bovine PTH-(3-34), and human PTH-(13-34) increased PKC activity in a dose-dependent manner. All fragments tested stimulated PKC at physiological concentrations (10(-11)-10(-10) M). Rat PTH-(1-34) (10(-7) M) increased PKA activity 4.5-fold, but other fragments failed to stimulate PKA between 10(-12)-10(-6) M. Human PTH-(28-34) stimulation of PKC was variable from experiment to experiment. All four PTH fragments tested increased 1,25-(OH)2D3 secretion by perifused renal proximal tubules at the lowest concentrations that stimulated PKC activity. The adenylate cyclase inhibitor 2',5'-dideoxyadenosine (10(-4) M) reduced PTH-(1-34)-stimulated PKA activity by 60%, but failed to block the rise in 1,25-(OH)2D3 secretion. The results of these studies demonstrate that PTH fragments that contain the PKC translocating domain stimulate 1,25-(OH)2D3 secretion, whereas elimination of the PKA activation domain does not alter the potency of the analogs' 1,25-(OH)2D3-stimulating activity. These results support the concept that PKC translocation may be required for PTH stimulation of 1,25-(OH)2D3 secretion.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Janulis M,Wong MS,Favus MJdoi
10.1210/endo.133.2.8344210subject
Has Abstractpub_date
1993-08-01 00:00:00pages
713-9issue
2eissn
0013-7227issn
1945-7170journal_volume
133pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Selective inhibitors of sodium glucose cotransporter-2 (SGLT2) are widely used for the treatment of type 2 diabetes and act primarily to lower blood glucose by preventing glucose reabsorption in the kidney. However, it is controversial whether these agents also act on the pancreatic islet, specifically the α cell, to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa080
更新日期:2020-08-01 00:00:00
abstract::Previous studies from this laboratory have demonstrated the presence of two populations of rat Leydig cells (I and II), which differ in their capacity for hCG- or cAMP-stimulated testosterone production. In the present study, we examined the metabolism of 25-hydroxycholesterol in primary cultures of both populations o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-1184
更新日期:1985-09-01 00:00:00
abstract::Exposure of rats to microsomal enzyme inducers perturbs thyroid hormone (TH) homeostasis through a variety of mechanisms. Glucuronidation is an important metabolic pathway for TH and is catalyzed by uridine diphosphate-dibenzo-glucuronosyltransferase (UGT) family proteins. Administration of 2,3,7,8-tetrachlorodibenzo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0443
更新日期:2007-12-01 00:00:00
abstract::Previous studies established a positive relationship between oocyte competence and follistatin mRNA abundance. Herein, we used the bovine model to test the hypothesis that follistatin plays a functional role in regulation of early embryogenesis. Treatment of early embryos with follistatin during in vitro culture (befo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1574
更新日期:2009-05-01 00:00:00
abstract::The development of white adipose tissue involves both the hypertrophy of existing adipocytes and the proliferation and differentiation of preadipocytes. Adipogenic differentiation is inhibited by TGFβ signaling through Smad2/3, and IGF binding protein-3 (IGFBP-3) is also known to activate Smad2/3 signaling in some cel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1444
更新日期:2012-10-01 00:00:00
abstract::Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells. Death factors such as Fas ligand induce apoptosis by triggering a series of intracellular protein-protein interactions mediated by defined motifs found in the signaling molecules. One of these motifs is the death effector d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6335
更新日期:1998-12-01 00:00:00
abstract::The effect of [D-Trp6]LHRH, a potent agonist of LHRH, on pituitary-testicular responses was investigated in male rhesus monkeys. Acute administration of 5, 25, or 500 micrograms [D-Trp6]LHRH produced dose-related increases in serum testosterone and bioassayable LH levels. The administration of 500 micrograms [D-Trp6]L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1308
更新日期:1982-04-01 00:00:00
abstract::Early life exposure to immunological challenge has programming effects on the adult hypothalamo-pituitary-adrenocortical axis stress responsivity, and stress is known to suppress GnRH pulse generator activity, especially LH pulses. We investigated the effects of neonatal exposure to endotoxin on stress-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0710
更新日期:2007-12-01 00:00:00
abstract::Although factors that regulate cAMP and steroid production in granulosa cells of hen preovulatory follicles have been well studied, much less is known of the mechanisms that control steroidogenesis in the adjacent thecal layer. These studies were conducted to examine the involvement and interaction of cAMP and protein...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1691
更新日期:1989-09-01 00:00:00
abstract::We have previously reported that the acute injection of interleukin-1 beta (IL-1 beta) into the brain ventricles of intact female rats promptly decreases LHRH release and inhibits gene expression of this peptide in the medial preoptic area (MPOA). The present studies were therefore designed to determine whether contin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243260
更新日期:1993-12-01 00:00:00
abstract::There is considerable evidence that CRH can suppress food intake. As hypothalamic CRH, a main site of CRH expression, is also negatively regulated by glucocorticoids, it is unclear whether anorexia and weight loss in adrenal insufficiency are attributable to elevated CRH or to decreased glucocorticoid levels. To disti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6416
更新日期:1999-01-01 00:00:00
abstract::We examined the effects of bombesin on rat pancreatic digestive enzyme gene expression using cloned complementary DNA probes for amylase, trypsinogen I, chymotrypsinogen B, and lysophospholipase. Rats were injected sc three times daily with 5 nmol/kg body wt bombesin. Pancreata were investigated after 6, 12, 24, 48, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.3.1371450
更新日期:1992-03-01 00:00:00
abstract::The influence of periparturient secretion of PRL on cytological differentiation of mammary epithelial cells was studied in 17 multiparous, monotocous Holstein cows. Mammary tissue was obtained 10 days prepartum and 10 days postpartum from untreated cows and from cows treated with CB154 (2-Br-alpha-ergokryptin) or CB15...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-31
更新日期:1981-07-01 00:00:00
abstract::A single injection of either natural (cortisol, corticosterone) or synthetic [dexamethasone (DEX), triamcinolone acetonide] glucocorticosteroids to estradiol-primed, withdrawn chicks, resulted in a dose-dependent increase in the relative rates of ovalbumin and conalbumin synthesis. The simultaneous injection of equal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2384
更新日期:1985-06-01 00:00:00
abstract::The estrogenic and antiestrogenic actions of the geometric isomers of tamoxifen and 4-hydroxytamoxifen were determined in a PRL synthesis assay using primary cultures of dispersed immature rat pituitary gland cells. 4-Hydroxytamoxifen was 100 times more potent as an antiestrogen than tamoxifen. The cis isomer of tamox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1449
更新日期:1988-04-01 00:00:00
abstract::The expression of cholesterol side-chain cleavage cytochrome P450 (P450scc), 17 alpha-hydroxylase cytochrome P450 (P45017 alpha), and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) was studied in bovine placenta and fetal adrenal glands throughout gestation. The levels of expression of these enzymes were much lower ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1374010
更新日期:1992-05-01 00:00:00
abstract::Inhibin was first characterized in mammals as a gonadal dimeric protein that inhibited pituitary follicle-stimulating hormone (FSH) secretion. As in mammals, the inhibin-specific α subunit (INHA/Inha/inha) has also been characterized in teleosts; however, its functions and physiological importance in fish reproduction...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa184
更新日期:2020-12-01 00:00:00
abstract::Glucose release from the liver is mediated by hypothalamic norepinephrine (NE) neuronal activity, but glucose itself (or a metabolite of it) exerts negative feedback effects on central NE activity. The aim of the present study was to investigate a possible role for insulin in these central glucose homeostatic mechanis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1590
更新日期:1985-10-01 00:00:00
abstract::Glucocorticoids and nonsteroidal antiinflammatory drugs (NSAIDs) are widely used for the treatment of inflammatory and immune diseases. Nitric oxide (NO) has a diversity of physiological functions, but its excess production has been implicated in the inflammatory process. The present study was designed to elucidate th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6718
更新日期:1999-05-01 00:00:00
abstract::Using messenger RNA (mRNA) differential display, we isolated several putative differentially expressed complementary DNAs (cDNAs) from the periimplantation (days 11-12) endometrium of unilaterally pregnant pigs. Nucleotide sequence analysis revealed that one cDNA clone was 87% homologous to human spermidine/ spermine ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.12.8940370
更新日期:1996-12-01 00:00:00
abstract::The ALP1 [aci-reductone dioxygenase (ARD)-like protein 1] gene was identified in a comprehensive cDNA subtraction aimed at identifying genes regulated by androgens in the rat ventral prostate. ALP1 is homologous to the ARD/ARD' that were discovered in Klebsiella pneumoniae as enzymes that have the same polypeptide seq...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0947
更新日期:2004-04-01 00:00:00
abstract::Light and electron microscopic triple immunostaining was performed on coronal vibratome sections prepared from the hypothalamus of ovariectomized (OVX) and OVX plus estrogen-treated African green monkeys (Cercopithecus aethiops). Immunoreactivity for progesterone receptors (PRs) and neuropeptide-Y (NPY) was visualized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8100520
更新日期:1993-07-01 00:00:00
abstract::Remodeling of maternal spiral arteries during pregnancy requires a subpopulation of extravillous cytotrophoblasts (EVTs) to differentiate into endovascular EVTs. Activin A, which is abundantly expressed at the maternal-fetal interface, has been shown to promote trophoblast invasion, but its role in endovascular differ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1228
更新日期:2015-11-01 00:00:00
abstract::Transglutaminase (TGase) activity, determined by incorporation of [1,4-14C]putrescine into casein, was demonstrated in a light membrane fraction derived from bovine calf testicular homogenates. In common with other TGases thus far identified, testicular TGase is calcium dependent. A concentration-dependent relationshi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-2-459
更新日期:1987-08-01 00:00:00
abstract::The tissue-specific differential processing of proglucagon (Pg) yields glucagon in pancreatic A cells and glucagon-like peptide-1 (GLP-1), GLP-2, and glicentin in intestinal L cells. It has been suggested that the difference in Pg cleavage in A and L cells is due to the presence of distinct prohormone convertases (PC)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.10.7068
更新日期:1999-10-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::Cross talks between the vascular and immune system play a critical role in vascular diseases, in particular in atherosclerosis. The osteoclast-associated receptor (OSCAR) is a regulator of osteoclast differentiation and dendritic cell maturation. Whether OSCAR plays a role in vascular biology and has an impact on athe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1282
更新日期:2011-12-01 00:00:00
abstract::Thyroid hormone entry into the thymocyte, a thyroid hormone target, was investigated by incubating the cells with tracer amounts of [125I]L-T3. At 37 C T3 uptake was linear with time up to 2 min, and then approached a plateau. The specific T3 uptake, obtained by subtracting the uptake in the presence of excess unlabel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2443
更新日期:1989-05-01 00:00:00
abstract::Gonadal differentiation is dependent upon a cascade of molecular and morphological events. Steroidogenic factor 1 (SF-1) and DAX-1 have been implicated in this process. A SF-1 binding site has been reported to be present on the DAX-1 gene. We therefore used immunocytochemistry to determine whether these two transcript...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754790
更新日期:1996-08-01 00:00:00
abstract::The Ca2+ response of individual cells to TRH was investigated in excitable pituitary GH3 and in nonexcitable Hela cells transfected with the TRH receptor complementary DNA (HelaR cells). GH3 cells typically responded to 1 microM TRH with an immediate transient [Ca2+]i spike (mean peak [Ca2+]i = 1.5 microM) followed by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070350
更新日期:1994-09-01 00:00:00