Abstract:
:The human breast cancer cell line MCF-7 responds to estrogens with increased progesterone receptor (PR) levels. In this study, we use dense amino acid density shift analyses to address directly the question of whether estrogen increases PR levels in MCF-7 cells by altering rates of receptor synthesis and/or degradation. Using different concentrations of estradiol (E2), which achieve PR levels that are half-maximal (3 X 10(-11) M F2) or maximal (6 X 10(-11) M E2), we have done sucrose gradient density shift analyses using dense (15N, 13C, 2H) amino acid incorporation to study rates of PR synthesis and degradation. These studies reveal a nonlinear loss of preexisting normal density receptor with time. From kinetic modeling analyses, equivalent rates of degradation are estimated for PR whether maximal or half-maximal levels are maintained, indicating that the major effect of E2 on PR content is to increase the rate of PR synthesis while leaving the degradation rate unaltered. The E2-stimulated increase in PR protein is also associated with increased levels of PR mRNA, as demonstrated by the use of a human PR cDNA probe. These density shift data provide evidence that the increased PR levels after estrogen exposure in MCF-7 cells are the result of an increased rate of receptor synthesis, rather than modulation of the rate of receptor degradation.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Nardulli AM,Greene GL,O'Malley BW,Katzenellenbogen BSdoi
10.1210/endo-122-3-935subject
Has Abstractpub_date
1988-03-01 00:00:00pages
935-44issue
3eissn
0013-7227issn
1945-7170journal_volume
122pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We reported previously that chromogranin A (Chga) knockout (KO) mice are hypertensive and hyperadrenergic. Here we sought to determine the basis of such alterations by probing physiological, biochemical, and pharmacological responses to perturbations of the autonomic nervous system. In the conscious state, KO mice had...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0429
更新日期:2009-11-01 00:00:00
abstract::Poor growth before birth is associated with impaired insulin sensitivity later in life, increasing the risk of type 2 diabetes. The tissue sites at which insulin resistance first develops after intrauterine growth restriction (IUGR), and its molecular basis, are unclear. We have therefore characterized the effects of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1955
更新日期:2012-05-01 00:00:00
abstract::The temporal aspects of gonadotropin release were studied in infantile (14-day-old) female rats. Blood samples were collected using vena cava cannulae at 10-, 15-, 20-, or 30-min intervals and assayed for either LH alone or for both LH and FSH. Within individual animals, blood LH levels exhibited a degree of variabili...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-5-1064
更新日期:1979-11-01 00:00:00
abstract::In view of recent reports on ovarian production and action of transforming growth factors (TGFs) and inhibin-related proteins (inhibin, activin, and follistatin), we have examined the effects of these hormones on the function of preovulatory follicles in vitro. Individual preovulatory follicles were obtained from PMSG...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-4-1857
更新日期:1989-10-01 00:00:00
abstract::We constructed seven chimeric molecules in which sequential segments in the cDNA for thyroid peroxidase (TPO) were replaced with the homologous regions of myeloperoxidase (MPO) cDNA. The sizes of the translated cDNA segments A through G ranged from 23-175 amino acid residues in length. The TPO-MPO cDNA chimeras, inser...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243270
更新日期:1993-12-01 00:00:00
abstract::The adrenal gland is highly vascularized with tightly regulated blood flow that is closely correlated with steroidogenesis. Mechanisms involved in the regulation of adrenal blood flow and vascular tone are largely unknown. The present study characterizes the contractile responses of isolated small cortical arteries fr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1448
更新日期:2004-04-01 00:00:00
abstract::An isolated perfused rabbit ovary preparation was used to determine the effects of cyanoketone, a potent inhibitor of 3 beta-hydroxysteroid dehydrogenase, on ovulation, ovum maturation and fertilizability, and steroid production. In the first experiment, cyanoketone (10(-4) M) was added to the perfusate of one ovary. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2555
更新日期:1987-06-01 00:00:00
abstract::As we previously showed, we have synthesized a new family of 17β-estradiol-platinum(II) hybrids. Earlier studies revealed the VP-128 hybrid to show high efficiency compared with cisplatin toward hormone-dependent breast cancer cells. In the present research, we have studied the antitumor activity of VP-128 in vitro an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1083
更新日期:2013-07-01 00:00:00
abstract::In rat uterus and prostate, 7 alpha, 17 alpha-dimethyl-19-nortestosterone (DMNT) binds to the androgen receptor specifically and with high affinity. However, this steroid does not bind to glucocorticoid receptors, since it does not displace binding of [3H]triamcinolone acetonide in calf thymus cytosol. In calf uterine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1327
更新日期:1986-04-01 00:00:00
abstract::Gonadal differentiation is dependent upon a cascade of molecular and morphological events. Steroidogenic factor 1 (SF-1) and DAX-1 have been implicated in this process. A SF-1 binding site has been reported to be present on the DAX-1 gene. We therefore used immunocytochemistry to determine whether these two transcript...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.8.8754790
更新日期:1996-08-01 00:00:00
abstract::The role of angiotensin II (AII) in human preadipocyte physiology has been investigated in primary cultures from human adipose tissue. Receptor binding studies indicated that human preadipocytes express a high affinity AII binding site of the AT1 subtype, as binding of 125I-labeled [Sar1,Ile8]AII was rapid, saturable,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.1.6430
更新日期:1999-01-01 00:00:00
abstract::The nature and role of the peptidergic innervation of the ovary were examined by determining the location and function of vasoactive intestinal peptide (VIP)-containing nerve fibers in the immature rat ovary. Immunohistofluorescence analysis of prepubertal ovaries using a specific VIP antibody revealed sparse delicate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1682
更新日期:1986-04-01 00:00:00
abstract::Reproductive function is dependent on the interaction between GnRH and its cognate receptor found on gonadotrope cells of the anterior pituitary gland. GnRH activation of the GnRH receptor (GnRHR) is a potent stimulus for increased expression of multiple genes including the gene encoding the GnRHR itself. Thus, homolo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220784
更新日期:2003-03-01 00:00:00
abstract::Levels of plasma prolactin were estimated in ovariectomized, estrogen-treated rats following the systemic administration of several neural blocking and stimulating drugs. Phenoxybenzamine, an alpha-adrenergic blocker, at high doses, increased plasma prolactin. Phenotlamine, another alpha-adrenergic blocker, and propra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-313
更新日期:1975-02-01 00:00:00
abstract::Aberrant expression of ectopic G protein-coupled receptors (GPCRs) in adrenal cortex tissue has been observed in several cases of ACTH-independent macronodular adrenal hyperplasias and adenomas associated with Cushing's syndrome. Although there is clear clinical evidence for the implication of these ectopic receptors ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0921
更新日期:2006-02-01 00:00:00
abstract::The steroid hormone 1 alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] can elicit biological responses via a nongenomic pathway that involves rapid opening of the plasma membrane Ca2+ channels. There is also evidence that 1,25-(OH)2D3 influences insulin secretion in the pancreatic beta-cell, which is primarily mediated by ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.7.7789310
更新日期:1995-07-01 00:00:00
abstract::Peroxisome proliferator activated receptor (PPARγ) is a nuclear receptor transcription factor that regulates adipogenesis and energy homeostasis. Recent studies suggest PPARγ may mediate some of its metabolic effects through actions in the brain. We used a Cre-recombinase-dependent (using NestinCre) conditionally acti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1524
更新日期:2016-11-01 00:00:00
abstract::We have investigated the characteristics and properties of cholesterol esterase (CEase) in the rat adrenal to clarify the mechanism of synthesis of free cholesterol from esterified cholesterol. CEase activity was estimated using substrate vesicles which were sonicated mixtures of cholesteryl oleate and phosphatidylcho...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-932
更新日期:1981-03-01 00:00:00
abstract::Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis because a loss-of-function mutation in the SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1492
更新日期:2004-10-01 00:00:00
abstract::Prolactin (PRL) is an immunomodulatory hormone which promotes T-cell activation and proliferation. However, the intracellular mechanisms of this action in normal lymphocytes are unknown. Because the PRL receptor (PRLR) activates several signals also activated by the T-cell antigen receptor (TCR)/CD3 complex, we evalua...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5913
更新日期:1998-02-01 00:00:00
abstract::[14C]-3-O-Methyl-D-glucose transport in response to GH stimulation was measured in freshly prepared and cultured hepatocytes from hypophysectomized (hypox) adult female rats. Sugar transport in freshly isolated hepatocytes from hypox animals was stimulated by 10(-9) M human GH (hGH) and 10(-9) M rat GH (rGH) during 0-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-239
更新日期:1981-01-01 00:00:00
abstract::Flow cytometry was used to separate and identify Sertoli and germ cell populations in primary rat testicular cultures derived from animals of different ages on the basis of cell size and DNA and lipid content. Multiparameter fluorescent evaluation of each cell preparation resulted in the assignment of specific stainin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1310280
更新日期:1992-02-01 00:00:00
abstract::α7-Nicotinic acetylcholine receptor (α7nAChR) agonists confer protection against a wide variety of cytotoxic insults and suppress oxidative stress and apoptosis in various cell systems, including hepatocytes. We recently demonstrated that nicotine, when combined with a high-fat diet (HFD), triggers oxidative stress, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00594
更新日期:2018-02-01 00:00:00
abstract::The hepatic extractions of gastric inhibitory polypeptide (GIP) and insulin were determined using in vitro and in vivo methods to assess the role of the liver in GIP metabolism and the possible effect of GIP on the hepatic extraction of insulin. During in vitro studies using the isolated perfused rat liver, infusion o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1011
更新日期:1984-09-01 00:00:00
abstract::The effect of delta 9-tetrahydrocannabinol (THC) on suckling-induced oxytocin release was investigated by recording intramammary pressure changes in suckled rats treated iv with THC (0.5 mg/kg BW) or vehicle. Latency to the first posttreatment milk ejection and posttreatment milk ejection intervals and pressure wave a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-469
更新日期:1988-07-01 00:00:00
abstract::Mutations in Na(+)-glucose transporters (SGLT)-2 and hepatocyte nuclear factor (HNF)-1alpha genes have been related to renal glycosuria and maturity-onset diabetes of the young 3, respectively. However, the expression of these genes have not been investigated in type 1 and type 2 diabetes. Here in kidney of diabetic r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1088
更新日期:2008-02-01 00:00:00
abstract::A diurnal change of tuberoinfundibular dopaminergic (TIDA) neuronal activity exists in female rats, which is prerequisite for the estrogen-induced afternoon PRL surge. Because progesterone (P4) administered in the morning can advance and amplify the PRL surge, it is of interest to learn whether its action involves the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5901
更新日期:1998-04-01 00:00:00
abstract::The Elovl3 gene belongs to the Elovl gene family, which encodes for enzymes involved in the elongation of very long chain fatty acids. The recognized role for the enzyme is to control the elongation of saturated and monounsaturated fatty acids up to 24 carbons in length. Elovl3 was originally identified as a highly ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1402
更新日期:2008-06-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) is a multifunctional cytokine that is thought to play a major role in the regulation of growth and differentiation of thyroid cells. However, little is known of its detailed mechanisms of action in thyrocytes. We have therefore studied the molecular mechanisms of TGF-beta1 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7918
更新日期:2001-01-01 00:00:00
abstract::The activity of the Na+/H+ exchanger was studied by measuring the effects of intracellular pH (pHi) and extracellular Na+ [(Na+)o] on pHi recovery and 22Na uptake in rat adipocytes. The resting pHi was acidified from 7.30 +/- 0.02 to 6.99 +/- 0.01 with nigericin in the absence of (Na+)o. pHi recovery induced by 30 mM ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.7.7789340
更新日期:1995-07-01 00:00:00