Abstract:
:Specific binding of human chorionic gonadotropin (hCG), and the hCG-sensitive adenyl cyclase of granulosa cells from small (1-2 mm), medium (3-5 mm), and large (6-12 mm) porcine ovarian follicles have been studied. The number of hCG-binding sites per cell (n) increases during follicle maturation without a change in the binding affinity. The values for n were 300-400 for small, 1,000-1,600 for medium, and 8,200-10,000 for large-follicle cells. The dissociation constant is 2.4 X 10(-10)M for all cells. hCG-sensitive adenyl cyclase was demonstrated in porcine granulosa cells. The adenyl cyclase system of granulosa cells becomes increasingly responsive to hCG stimulation during follicle development. Maximal adenyl cyclase activation by hCG (1 mug/ml) was 240, 750, and 7,000 molecules of cyclic AMP formed/sec/cell, respectively, for small, medium, and large follicle cell. The concentration of hCG giving half-maximal stimulation (1.0 X 10(-9)M) was similar for both large and medium follicle cells. It is concluded that: 1) an increase in hCG receptor sites per cell occurs during maturation of the porcine ovarian follicle without change of binding affinity, and 2) the increase in the number of hCG receptors correlates well with hCG-sensitive adenyl cyclase activity during follicle development.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lee CYdoi
10.1210/endo-99-1-42subject
Has Abstractpub_date
1976-07-01 00:00:00pages
42-8issue
1eissn
0013-7227issn
1945-7170journal_volume
99pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The GnRH receptor (GnRH-R) belongs to the rhodopsin/beta-adrenergic family of G protein-coupled receptors. The intracellular domains of these receptors, particularly the regions closest to the plasma membrane in intracellular loops 2 (2i) and 3 (3i) as well as some regions located in the membrane-proximal end of the C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6022
更新日期:1998-05-01 00:00:00
abstract::Using a primary culture of neonatal rat heart, we investigated the properties of iodothyronine 5'-deiodinating activity in the heart. 125I- release from [125I] reverse T3 incubated with cell homogenates depended on the concentrations of protein and dithiothreitol, pH, incubation time, and temperature of the incubation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3105
更新日期:1991-06-01 00:00:00
abstract::The quantitative light microscope radioautographic approach was utilized to locate specific binding sites for blood-borne 125I-labeled ACTH1-24 in the rat brain. Specific binding occurred exclusively in the external zone of the median eminence, adjacent to the primary portal plexus, and to a lesser extent in the ventr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-6-2385
更新日期:1981-06-01 00:00:00
abstract::Treatment of isolated rat adipocytes with epinephrine or isoproterenol caused a time- and concentration-dependent increase in phospholipid methyltransferase (PLMT) activity that was blocked by propranolol and unaffected by phentolamine. Forskolin mimicked the stimulatory effect on PLMT, and insulin inhibited this effe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2421
更新日期:1987-06-01 00:00:00
abstract::The adrenal gland is highly vascularized with tightly regulated blood flow that is closely correlated with steroidogenesis. Mechanisms involved in the regulation of adrenal blood flow and vascular tone are largely unknown. The present study characterizes the contractile responses of isolated small cortical arteries fr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1448
更新日期:2004-04-01 00:00:00
abstract::Most of the constitutive hepatic P450 isoforms expressed in the rat exhibit dramatic gender differences. Whereas only male hepatocytes contain CYP2A2, 2C11, and 3A2, only female hepatocytes express CYP2C12 and 3- to 4-fold greater levels of CYP2C7. This sexually dimorphic expression of hepatic P450 isoforms is regulat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.3.6545
更新日期:1999-03-01 00:00:00
abstract::A RIA for mouse placental lactogen-I (mPL-I) was developed using recombinant mPL-I as the standard, radioligand, and antigen for antiserum production. Displacement curves for dilutions of serum and placental extracts from pregnant mice were parallel to the recombinant mPL-I standard curve. Serum from male and nonpregn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2253
更新日期:1989-11-01 00:00:00
abstract::An estrogen-binding macromolecule was identified in cytosolic fractions prepared from fetal rat lung tissue. This component sedimented at 4.5S on sucrose gradients and was heat labile and proteinaceous in nature. This estrogen-binding protein bound 17 beta-estradiol (Ka = 10(8) M-1) and estrone but had little or no af...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-210
更新日期:1981-07-01 00:00:00
abstract::We analyzed the mechanism by which a Graves' disease-associated C/T polymorphism in the Kozak sequence of CD40 affects CD40 expression. CD40 expression levels on B cells in individuals with CT and TT genotypes were decreased by 13.3 and 39.4%, respectively, compared with the levels in CC genotypes (P = 0.012). Similar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1617
更新日期:2005-06-01 00:00:00
abstract::Mesenchymal stroma/stem cells (MSCs) exist in adult tissues, such as adipose tissue and bone marrow, and differentiate into cells of multiple lineages. In previous studies, we found that MSCs differentiate into steroidogenic cells by forced expression of steroidogenic factor 1 (SF-1)/adrenal 4 binding protein (Ad4BP),...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa007
更新日期:2020-03-01 00:00:00
abstract::Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells. Death factors such as Fas ligand induce apoptosis by triggering a series of intracellular protein-protein interactions mediated by defined motifs found in the signaling molecules. One of these motifs is the death effector d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6335
更新日期:1998-12-01 00:00:00
abstract::Brown adipose tissue (BAT) thermogenesis is activated by the sympathetic nervous system. BAT responses to norepinephrine are blunted in hypothyroidism and are rapidly restored by thyroid hormone. We examined in rats the effects of thyroid hormone on BAT beta 1- and beta 2-adrenergic receptors (AR) expression and capac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628360
更新日期:1995-08-01 00:00:00
abstract::The distribution of peptide YY (PYY)-like immunoreactivity (IR) in rat tissues was determined by specific RIA after extraction with boiled 1 N acetic acid. The high concentration of PYY-IR was observed in the gastrointestinal tract, with concentrations gradually increasing from the duodenum to the end of colon. The co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2163
更新日期:1986-06-01 00:00:00
abstract::The administration of testosterone via Silastic capsules has been shown previously to maintain advanced spermatid number quantitatively in intact rats in which LH but not FSH was suppressed, but not in hypophysectomized rats, indicating that pituitary factors in addition to LH are required for the quantitative mainten...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597140
更新日期:1992-06-01 00:00:00
abstract::Serum retinol-binding protein 4 (RBP4) levels are increased in insulin-resistant humans and correlate with severity of insulin resistance in metabolic syndrome. Quantitative Western blotting (qWestern) has been the most accurate method for serum RBP4 measurements, but qWestern is technically complex and labor intensiv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1750
更新日期:2012-03-01 00:00:00
abstract::In female mammals, reproduction is sensitive to the availability of metabolic fuels, and food deprivation has been shown to suppress pulsatile LH secretion, attenuate the preovulatory LH surge, and prevent ovulation. It has been suggested that food deprivation impairs fertility by reducing the secretion of GnRH by GnR...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1425420
更新日期:1992-11-01 00:00:00
abstract::To examine the role of androgen receptor (AR) in Sertoli cells (SC), we used a SC-specific AR knockout (S-AR-/y) mouse to further evaluate the chronological changes of seminiferous tubules and the molecular mechanisms of SC androgen/AR signals on spermatogenesis. Testes morphology began changing as early as postnatal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0138
更新日期:2006-12-01 00:00:00
abstract::The SMRT coregulator functions as a dual coactivator and corepressor for estrogen receptor-α (ERα) in a gene-specific manner, and in several studies its elevated expression correlates with poor outcome for breast cancer patients. A specific role of SMRT in breast cancer progression has not been elucidated, but SMRT kn...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1002
更新日期:2014-09-01 00:00:00
abstract::Members of the winged helix transcription factor family are known to regulate epithelial cell differentiation by regulating cell-specific gene expression. rWIN is a newly discovered member of the winged helix family shown to be present in the adult rat testis. In the testis the human homolog of rWIN, HFH-11, was local...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.8.7599
更新日期:2000-08-01 00:00:00
abstract::Sodium vanadate, an agent known to have multiple cellular actions, was studied for its effects on aspects of bone formation in cultures of 21-day-old fetal rat calvariae. Vanadate (0.1-10 microM) stimulated the incorporation of [3H] thymidine into acid-insoluble residues (DNA); the effect appeared after 3 h and was su...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-855
更新日期:1985-03-01 00:00:00
abstract::Analysis of GH gene structure in the mouse permits evolutionary comparisons with GH gene structure in the rat and provides information about the mechanism responsible for heritable deficiencies of anterior pituitary hormones. The little mutation in mice is analogous to autosomal recessive, isolated, partial deficiency...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-5-1672
更新日期:1982-05-01 00:00:00
abstract::Pulmonary surfactant is a developmentally and hormonally regulated lipoprotein synthesized exclusively in alveolar type II cells. Surfactant protein-A (SP-A) gene transcription in human fetal lung in culture is stimulated by glucocorticoids and cAMP; cAMP also enhances the rate of type II cell differentiation. The CCA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5637
更新日期:1997-12-01 00:00:00
abstract::Serum levels of progesterone in hysterectomized gilts were determined to evaluate the effects of exogenous 17 beta-estradiol (17 beta-E2) on the secretory activity of aging corpora lutea. Gilts were hysterectomized on day 6 of the estrous cycle and given im injections of 17 beta-E2 to mimic blood levels of endogenous ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-5-1876
更新日期:1984-11-01 00:00:00
abstract::Kisspeptin neurons play an essential role in the regulation of fertility through direct regulation of the GnRH neurons. However, the relative contributions of the two functionally distinct kisspeptin neuron subpopulations to this critical regulation are not fully understood. Here we analyzed the specific projection pa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1131
更新日期:2015-07-01 00:00:00
abstract::We studied the estrogen-dependent expression of epidermal growth factor (EGF), transforming growth factor (TGF) alpha, and EGF receptor gene transcripts in human fallopian tubes in vivo and in vitro. Competitive polymerase chain reaction (PCR) was performed on the fallopian tube RNA samples from the postmenopausal wom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720660
更新日期:1995-05-01 00:00:00
abstract::Ca2+ influx through voltage-gated Ca2+ channels (VGCCs) plays a key role in GH secretion. In this review, we summarize the current state of knowledge regarding the physiology and molecular machinery of VGCCs in pituitary somatotrophs. We next discuss the possible involvement of Ca2+ channelopathies in pituitary diseas...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2018-00743
更新日期:2018-12-01 00:00:00
abstract::Testicular peritubular cells are myofibroblastic cells, which represent the major cellular components of the wall of the seminiferous tubules. In men their phenotypic characteristics, including possible secretory activity and regulation, are not well known, in neither normal nor pathologically altered testes. Especial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1064
更新日期:2008-04-01 00:00:00
abstract::The endoplasmic reticulum (ER) mediates the first steps of protein assembly within the secretory pathway and is the site where protein folding and quality control are initiated. The storage and release of Ca2+ are critical physiological functions of the ER. Disrupted ER homeostasis activates the unfolded protein respo...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqz028
更新日期:2020-02-01 00:00:00
abstract::Studies on hormone-receptor interactions generally assume that the formation of a hormone-receptor complex is a reversible process. This assumption has been examined directly in three experiments using liver membrane receptor preparations from pregnant rats and ovine PRL (oPRL). In Exp 1, Receptors were preincubated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-1-402
更新日期:1980-01-01 00:00:00
abstract::Hypoxia, a common stressor in prematurity, leads to sexually dimorphic, short- and long-term effects on the adult hypothalamic-pituitary-adrenal (HPA) and hypothalamic-pituitary-gonadal (HPG) axes. We hypothesized that these effects are due to stress-induced increases in testosterone during early postnatal life. We ev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa085
更新日期:2020-07-01 00:00:00