Abstract:
:The UDP-GalNAc:NeuAc alpha 2,3Gal beta-R (GalNAc to Gal) beta-1,4-N-acetylgalactosaminyltransferase (beta 1,4GalNAc-transferase) is the enzyme responsible for the addition of the immunodominant sugar of the Sda isto-blood group determinant. In humans the enzyme is mainly expressed in the large intestine. We screened nine human colorectal carcinoma cell lines (SW-948, SW-948 FL, SW-480, SW-48, SW-1417, COLO-205, LOVO, HT-29 and CaCo-2) in order to ascertain the occurrence of beta 1,4GalNAc-transferase. Only in CaCo-2 cells the glycosyltransferase was detected and the activity increased with the degree of enterocytic differentiation. Nevertheless, in highly differentiated CaCo-2 cells the activity was thirty times lower than that found in cells detached from normal colon mucosa. These results support the notion that the expression of beta 1,4GalNAc-transferase is a marker of the colonic cell maturation.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Malagolini N,Dall'Olio F,Serafini-Cessi Fdoi
10.1016/s0006-291x(05)81119-2subject
Has Abstractpub_date
1991-10-31 00:00:00pages
681-6issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(05)81119-2journal_volume
180pub_type
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journal_title:Biochemical and biophysical research communications
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