Tissue-engineered skin containing mesenchymal stem cells improves burn wounds.

Abstract:

:There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combined with bone MSCs, we established a burn wound model in the porcine skin. With a controlling temperature and time of the burning device to obtain different degrees of burn wounds, a deep dermal partial thickness burn was introduced to the porcine skin using a heated-brass contact injury at 100 degrees C for 20 s. Collagen-GAG scaffolds were utilized as the matrix; MSCs separated from pigs were seeded on them to form the skin equivalent. When grafted to the burn wounds, the TE skin containing MSCs showed better healing and keratinization, less wound contraction, and more vascularization. Grafts proliferated well and contributed to the neo-tissues. These data suggest that TE skin containing MSCs in a burn defect can accelerate wound healing and receive satisfactory effects.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Liu P,Deng Z,Han S,Liu T,Wen N,Lu W,Geng X,Huang S,Jin Y

doi

10.1111/j.1525-1594.2008.00654.x

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

925-31

issue

12

eissn

0160-564X

issn

1525-1594

pii

AOR654

journal_volume

32

pub_type

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